An increased proportion of Gram-negative bacteria have recently been reported among etiologic agents of infection. In Poland, Acinetobacter baumannii is a big problem for hospitals, especially intensive care units. Touch surfaces made from materials with antimicrobial properties, especially copper alloys, are recommended as a supplementary method of increasing biological safety in the hospital environment.
The objective of this study is to determine the susceptibility to selected copper alloys of three clinical Acinetobacter baumannii strains, one Acinetobacter lwoffi and an A. pittii strain isolated from the hospital environment.
The modification of the Japanese Standard, which the ISO 22196:2011 norm was used for testing antimicrobial properties of CuZn37, CuSn6 and CuNi18Zn20 and Cu-ETP and stainless steel as positive and negative control, respectively.
The highest cidal efficiency, expressed as both time and the degree of reduction of the initial suspension density, against all of the tested Acinetobacter strains was found for ETP copper. But, the results of our study also confirmed effective activity (bacteriocidal or bacteriostatic) of copper alloys selected for the study, contrary to the stainless steel. The reduction in bacterial suspension density is significantly different depending on the strain and copper alloy composition.
The results of our study confirmed the effective antibacterial activity of copper and its selected alloys against clinical Acinetobacter baumannii and Acinetobacter lwoffii strains, and Acinetobacter pittii strain isolated from the hospital environment.
ECDC. European Center for Disease Prevention and Control. Surveillance and disease data for antimicrobial resistance. Available: http://ecdc.europa.eu/en/healthtopics/antimicrobial-resistance-and-consumption/antimicrobial_resistance/EARS-Net/Pages/EARS-Net.aspx
Chmielarczyk A, Pilarczyk-Żurek M, Kamińska W, Pobiega M, Romaniszyn D, Ziółkowski G, et al. Molecular epidemiology and drug resistance of Acinetobacter baumannii isolated from hospitals in southern Poland: ICU as a risk factor for XDR strains. Microb Drug Resist. 2016;22(4):328–35. https://doi.org/10.1089/mdr.2015.0224. CrossRefPubMed
United States Environmental Protection Agency. Test Method for Efficacy of Copper Alloys Surfaces as a Sanitizer. Available online: http://www.antimicrobialcopper.org/sites/default/files/upload/media-library/files/pdfs/us/epa_sanitizer_test_method_copper_alloy_surfaces.pdf [Accessed on 21 Apr 2017].
Japanese Industrial Standard JIS Z 2801 “Antimicrobial products – Test for antibacterial activity and efficacy” https://law.resource.org/pub/jp/ibr/jis.z.2801.e.2010.pdf [Accessed 21 Apr 2017].
Różańska A, Romaniszyn D, Chmielarczyk A, Bulanda M. Contamination with bacteria of otuch surfaces inPolish hospital wards. Med Pr. 2017;68(3) https://doi.org/10.13075/mp.5893.00575.
Magiorakos AP, Srinivasan A, Carey RB, Carmeli Y, Falagas MA, Giske CG, et al. Multidrugresistant, extensively drug-resistant and pan-drug-resistance bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clin Microbial Infect. 2012;18:268–81. CrossRef
Souli M, Galani I, Plachouras D, Panagea T, Armaganidis A, Petrikkos G, et al. Antimicrobial activity of copper surfaces against carbapenemase-producing contemporary gram-negative clinical isolates. J Antimicrob Chemother. 2012; https://doi.org/10.1093/jac/dks473.
Różańska A, Chmielarczyk A, Romaniszyn D, Sroka-Oleksiak A, Bulanda M, Walkowicz M, et al. Antimicrobial properties of selected copper alloys on Staphylococcus Aureus and Escherichia Coli in different simulations of environmental conditions: with vs. without organic contamination. Int J Environ Res Public Health. 2017;14(7) https://doi.org/10.3390/ijerph14070813.
Różańska A, Chmielarzyk A, Romaniszyn D, Bulanda M, Walkowicz M, Osuch P, et al. Antibiotic resistance, ability to form biofilm and susceptibility to copper alloys of selected staphylococcal strains isolated from touch surfaces in polish hospital wards. Antimicrob Resist Infect Control. 2017;6:80. https://doi.org/10.1186/s13756-017-0240-x. eCollection 2017 CrossRefPubMedPubMedCentral
Steindl G, Heuberger S, Springer B. Antimicrobial effect of copper on multidrug-resistant bacteria. Vet Med Austria. 2012;99:38–43.
Warnes SL, Caves V, Keevil CW. Mechanism of copper surfaces toxicity in Escherichia coli O157:H7 and Salmonella involves immediate membrane depolarization followed by slower rate of DNA destruction which differs from that observed for gram-positive bacteria. Environ Microb. 2012;14(7):1730–43. CrossRef
Hong R, Kang I, Chidaine B, Cariolet R, Ersboll AK, Houe H, et al. Copper resistance in Enterococcus faecium mediated by the tcrB gene, is selected by supplementation of pig feed with copper sulfate. Appl Environ Microbiol. 2006;72(9):5784–9. CrossRef
Yeh A-C, Huang C-C, Hsiao C-C, Chu T-W, Yang Y-C, Feng Y-W, Lin K-Y. Some aspects on the discoloration and antimicrobial property of a thermally passivated copper surface in a highly humid environment. Mater Trans. 2011;52:265–7. CrossRef
- Antimicrobial effect of copper alloys on Acinetobacter species isolated from infections and hospital environment
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