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Archives of Virology

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11.01.2018 | Original Article

Antiviral effect of lithium chloride on replication of avian leukosis virus subgroup J in cell culture

verfasst von: Kun Qian, Xiaowei Cheng, Danyang Zhang, Hongxia Shao, Yongxiu Yao, Venugopal Nair, Aijian Qin

Erschienen in: Archives of Virology | Ausgabe 4/2018

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Abstract

Lithium chloride (LiCl) has been reported to possess antiviral activity against several viruses. In the current study, we assessed the antiviral activity effect of LiCl on ALV-J infection in CEF cells by using real-time PCR, Western blot analysis, IFA and p27 ELISA analysis. Our results showed that both viral RNA copy number and protein level decreased significantly in a dose and time dependent manner. Time-course analysis revealed that the antiviral effect was more pronounced when CEFs were treated at the post infection stage rather than at early absorption or pre-absorption stages. Further experiments demonstrated that LiCl did not affect virus attachment or entry, but rather affected early virus replication. We also found that inhibition of viral replication after LiCl treatment was associated with reduced mRNA levels of pro-inflammatory cytokines. These results demonstrate that LiCl effectively blocked ALV-J replication in CEF cells and may be used as an antiviral agent against ALV-J.

Ballanger F, Tenaud I, Volteau C, Khammari A, Dreno B (2008) Anti-inflammatory effects of lithium gluconate on keratinocytes: a possible explanation for efficiency in seborrhoeic dermatitis. Arch Dermatol Res 300(5):215–223PubMedCrossRef

Baril M, Es-Saad S, Chatel-Chaix L, Fink K, Pham T, Raymond VA, Audette K, Guenier AS, Duchaine J, Servant M, Bilodeau M, Cohen E, Grandvaux N, Lamarre D (2013) Genome-wide RNAi screen reveals a new role of a WNT/CTNNB1 signaling pathway as negative regulator of virus-induced innate immune responses. PLoS Pathog 9(6):e1003416PubMedPubMedCentralCrossRef

Chen K, Wu Y, Zhu M, Deng Q, Nie X, Li M, Wu M, Huang X (2013) Lithium chloride promotes host resistance against Pseudomonas aeruginosa keratitis. Mol Vis 19:1502–1514PubMedPubMedCentral

Chen Y, Kong D, Cai G, Jiang Z, Jiao Y, Shi Y, Li H, Wang C (2016) Novel antiviral effect of lithium chloride on mammalian orthoreoviruses in vitro. Microb Pathog 93:152–157PubMedCrossRef

Chen Y, Yan H, Zheng H, Shi Y, Sun L, Wang C, Sun J (2015) Antiviral effect of lithium chloride on infection of cells by porcine parvovirus. Adv Virol 160(4):1015–1020

Cui J, Xie J, Gao M, Zhou H, Chen Y, Cui T, Bai X, Wang H, Zhang G (2015) Inhibitory effects of lithium chloride on replication of type II porcine reproductive and respiratory syndrome virus in vitro. Antiviral Ther 20(6):565–572CrossRef

Cui Z, Sun S, Zhang Z, Meng S (2009) Simultaneous endemic infections with subgroup J avian leukosis virus and reticuloendotheliosis virus in commercial and local breeds of chickens. Avian Pathol 38(6):443–448PubMedCrossRef

De Meyer I, Martinet W, Van Hove CE, Schrijvers DM, Hoymans VY, Van Vaeck L, Fransen P, Bult H, De Meyer GR (2011) Inhibition of inositol monophosphatase by lithium chloride induces selective macrophage apoptosis in atherosclerotic plaques. Br J Pharmacol 162(6):1410–1423PubMedPubMedCentralCrossRef

Ding Y, Dou J, Teng Z, Yu J, Wang T, Lu N, Wang H, Zhou C (2014) Antiviral activity of baicalin against influenza A (H1N1/H3N2) virus in cell culture and in mice and its inhibition of neuraminidase. Adv Virol 159(12):3269–3278

10.

Everall IP, Bell C, Mallory M, Langford D, Adame A, Rockestein E, Masliah E (2002) Lithium ameliorates HIV-gp120-mediated neurotoxicity. Mol Cell Neurosci 21(3):493–501PubMedCrossRef

11.

Hao HP, Wen LB, Li JR, Wang Y, Ni B, Wang R, Wang X, Sun MX, Fan HJ, Mao X (2015) LiCl inhibits PRRSV infection by enhancing Wnt/beta-catenin pathway and suppressing inflammatory responses. Antiviral Res 117:99–109PubMedCrossRef

12.

Harrison SM, Tarpey I, Rothwell L, Kaiser P, Hiscox JA (2007) Lithium chloride inhibits the coronavirus infectious bronchitis virus in cell culture. Avian Pathol 36(2):109–114PubMedCrossRef

13.

Hu X, Ye J, Qin A, Zou H, Shao H, Qian K (2015) Both MicroRNA-155 and virus-encoded MiR-155 ortholog regulate TLR3 expression. PLoS One 10(5):e0126012PubMedPubMedCentralCrossRef

14.

Kumar A, Zloza A, Moon RT, Watts J, Tenorio AR, Al-Harthi L (2008) Active beta-catenin signaling is an inhibitory pathway for human immunodeficiency virus replication in peripheral blood mononuclear cells. J Virol 82(6):2813–2820PubMedPubMedCentralCrossRef

15.

Letendre SL, Woods SP, Ellis RJ, Atkinson JH, Masliah E, van den Brande G, Durelle J, Grant I, Everall I (2006) Lithium improves HIV-associated neurocognitive impairment. AIDS (London, England) 20(14):1885–1888CrossRef

16.

Li X, Lin W, Chang S, Zhao P, Zhang X, Liu Y, Chen W, Li B, Shu D, Zhang H, Chen F, Xie Q (2016) Isolation, identification and evolution analysis of a novel subgroup of avian leukosis virus isolated from a local Chinese yellow broiler in South China. Adv Virol 161(10):2717–2725

17.

Li X, Liu Y, Wu T, Jin Y, Cheng J, Wan C, Qian W, Xing F, Shi W (2015) The antiviral effect of baicalin on enterovirus 71 in vitro. Viruses 7(8):4756–4771PubMedPubMedCentralCrossRef

18.

Li YP, Bang DD, Handberg KJ, Jorgensen PH, Zhang MF (2005) Evaluation of the suitability of six host genes as internal control in real-time RT-PCR assays in chicken embryo cell cultures infected with infectious bursal disease virus. Vet Microbiol 110(3–4):155–165PubMedCrossRef

19.

Machado-Vieira R, Manji HK, Zarate CA Jr (2009) The role of lithium in the treatment of bipolar disorder: convergent evidence for neurotrophic effects as a unifying hypothesis. Bipolar Disord 11(Suppl 2):92–109PubMedPubMedCentralCrossRef

20.

Mei M, Ye J, Qin A, Wang L, Hu X, Qian K, Shao H (2015) Identification of novel viral receptors with cell line expressing viral receptor-binding protein. Sci Rep 5:7935PubMedPubMedCentralCrossRef

21.

Nunes PV, Forlenza OV, Gattaz WF (2007) Lithium and risk for Alzheimer’s disease in elderly patients with bipolar disorder. Br J Psychiatry 190:359–360PubMedCrossRef

22.

Oruch R, Elderbi MA, Khattab HA, Pryme IF, Lund A (2014) Lithium: a review of pharmacology, clinical uses, and toxicity. Eur J Pharmacol 740:464–473PubMedCrossRef

23.

Payne LN, Nair V (2012) The long view: 40 years of avian leukosis research. Avian Pathol 41(1):11–19PubMedCrossRef

24.

Pietruczuk K, Jozwik A, Ruckemann-Dziurdzinska K, Bryl E, Witkowski JM (2009) Cytoprotective effect of lithium against spontaneous and induced apoptosis of lymphoid cell line MOLT-4. Folia Histochem Cytobiol 47(4):639–646PubMed

25.

Qian K, Gao AJ, Zhu MY, Shao HX, Jin WJ, Ye JQ, Qin AJ (2014) Genistein inhibits the replication of avian leucosis virus subgroup J in DF-1 cells. Virus Res 192:114–120PubMedCrossRef

26.

Ren X, Meng F, Yin J, Li G, Li X, Wang C, Herrler G (2011) Action mechanisms of lithium chloride on cell infection by transmissible gastroenteritis coronavirus. PLoS One 6(5):e18669PubMedPubMedCentralCrossRef

27.

Rychlik I, Karasova D, Sebkova A, Volf J, Sisak F, Havlickova H, Kummer V, Imre A, Szmolka A, Nagy B (2009) Virulence potential of five major pathogenicity islands (SPI-1 to SPI-5) of Salmonella enterica serovar Enteritidis for chickens. BMC Microbiol 9:268PubMedPubMedCentralCrossRef

28.

Singh N, Halliday AC, Thomas JM, Kuznetsova OV, Baldwin R, Woon EC, Aley PK, Antoniadou I, Sharp T, Vasudevan SR, Churchill GC (2013) A safe lithium mimetic for bipolar disorder. Nat Commun 4:1332PubMedPubMedCentralCrossRef

29.

Skinner GR, Hartley C, Buchan A, Harper L, Gallimore P (1980) The effect of lithium chloride on the replication of herpes simplex virus. Med Microbiol Immunol 168(2):139–148PubMedCrossRef

30.

Wang F, Wang X, Chen H, Liu J, Cheng Z (2011) The critical time of avian leukosis virus subgroup J-mediated immunosuppression during early stage infection in specific pathogen-free chickens. J Vet Sci 12(3):235–241PubMedPubMedCentralCrossRef

31.

Wang X, Zhao P, Cui ZZ (2012) Identification of a new subgroup of avian leukosis virus isolated from Chinese indigenous chicken breeds. Chin J Virol 28(6):609–614

32.

Wu X, Qian K, Qin A, Shen H, Wang P, Jin W, Eltahir YM (2010) Recombinant avian leukosis viruses of subgroup J isolated from field infected commercial layer chickens with hemangioma and myeloid leukosis possess an insertion in the E element. Vet Res Commun 34(7):619–632PubMedPubMedCentralCrossRef

33.

Zhao FR, Xie YL, Liu ZZ, Shao JJ, Li SF, Zhang YG, Chang HY (2017) Lithium Chloride inhibits early stages of foot-and-mouth disease virus (FMDV) replication in vitro. J Med Virol

34.

Zhou P, Fu X, Yan Z, Fang B, Huang S, Fu C, Hong M, Li S (2015) Antiviral effect of lithium chloride on infection of cells by canine parvovirus. Adv Virol 160(11):2799–2805

35.

Ziaie Z, Kefalides NA (1989) Lithium chloride restores host protein synthesis in herpes simplex virus-infected endothelial cells. Biochem Biophys Res Commun 160(3):1073–1078PubMedCrossRef

Titel: Antiviral effect of lithium chloride on replication of avian leukosis virus subgroup J in cell culture
verfasst von: Kun Qian
Xiaowei Cheng
Danyang Zhang
Hongxia Shao
Yongxiu Yao
Venugopal Nair
Aijian Qin
Publikationsdatum: 11.01.2018
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 4/2018
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-017-3692-7

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Antiviral effect of lithium chloride on replication of avian leukosis virus subgroup J in cell culture

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