Background
Over the years, medicinal plants have been used to manage an array of diseases/ailments. Nearly 80% of the populations of the world still rely on local medicines and traditional treatments mainly from plant extracts [
1,
2]. It is noteworthy that herbal medicine is becoming very popular in the developing countries [
3]. Today, the Nigerian traditional medicines are administered to treat a myriad of health problems including mental disorders, insomnia, broken bones and infertility as well as other reproductive health challenges [
4].
It has been acclaimed that aphrodisiacs with a healthy lifestyle can achieve a better sexual life [
5]. Sexual feelings are an inevitable part of life. The sex is the most cherished, indispensable and an integral part of every individual and can be a cradle of pleasure and satisfaction. There has been erroneous information, unawareness, fear and pessimistic outlook as far as sex is concerned. Myths and misconceptions are widespread and are passed on from one generation to another. These sexual myths can results in sexual dysfunctions, misery, silent suffering, distressed interpersonal relationships and even divorce. Sexual ignorance is a social disease and can be solved via compulsory all-inclusive sex education. This can boost awareness and improve the society [
6].
However, aphrodisiac have been implicated in treating/managing these arrays of sexual disorders [
6‐
9]. Most aphrodisiacs can be said to amplify some facets of sensual experience such as light, touch, smell, taste and hearing. This improved sensual consciousness leads to sexual stimulation and inclination [
10]. Many locally accepted constituents have been known to increase libido in some continents of the world. Notable examples include Yohimbine, the Mandrake plant, ground Rhinoceros horn in the Chinese culture and the lethal “Spanish fly”. An aphrodisiac is any substance or agent (food, drug, scent or device) that stimulates the erotic instinct, induces veneral desire and surges pleasure and performance [
8,
11,
12]. They are agents that can change impaired sexual functions [
7]. Aphrodisiac substances can nevertheless be screened for activity by employing two methods: the observatory or physical method that encompasses the mating behavioural study (mount frequency and latency, intromission frequency and latency, ejaculatory latency and frequency, test for libido etc.) and biochemical method (hormonal determination, organ weight, histopathology, sperm count, motility and morphology etc). In either of the method, in vivo and in vitro animal models are used to ascertain aphrodisiac activity in laboratory animals such as rats, mice and guinea pigs [
13]. Several reports have also attested to the use of animals particularly rats in assessing aphrodisiac activity of medicinal plants as found in [
6,
7,
14‐
20].
Although, through history, a myriad of feature has qualified diverse substances as having aphrodisiac property, but are done generally via two probable approaches- cultural and scientific. Culturally, via the famous doctrine of signature, many plants and animals parts have been tented as having aphrodisiac effect. Popular among these, is the accepted belief of hunters of those eras when they consume definite parts of their prey to get the characteristics of those organs. In some part of the world (England and Ukraine), it is believed that plants with any Phallic-like characteristics such as asparagus, parsnips, celery and carrots were expected to have aphrodisiac property or effect. From our observation and as have been noted elsewhere, e.g. Rosen and Ashton [
21], the root of AB is phallic-like in nature and culturally can be said to have an aphrodisiac effect. Scientifically, based on mechanism of action, aphrodisiacs can be classified into three categories- (aphrodisiacs that offer a high level of nutritional value, those with specific physiological effect and those that are psychologically active in nature. Following the phytochemical and nutritional constituents described elsewhere ([
22], unpublished observation (Erhabor and Idu)), the extract of AB is said to have glycosides, flavonoid, phenol, tannin, alkaloids, steroids, reducing sugar, nitrogen free extract, crude fibre, crude protein, crude oil, ash content and moisture. Other nutritional components included sodium, potassium, magnesium, iron and zinc. Some of these phytoconstituents have been implicated for aphrodisiac activity in some plants (e.g. potassium, calcium, zinc and magnesium are known to be involved in processes that promote penile erection as reported by Jeon [
23], Ghofrani [
24], Adrogue and Madias [
25], Coleman [
26]. Alkaloids according to Zamblé et al. [
27] dilate blood vessel which physiologically results to increase in blood flow to the penile organ and engorging of the penis for sexual performance. Phenols found in clove (
Syzygium aromaticum) as observed by Tajuddin et al. [
28] was said to be responsible for the aphrodisiac property of the plant. Also increase in testicular and/or serum cholesterol levels have been shown to be linked to the aphrodisiac activity of a medicinal plant. This is sequel to cholesterol being the precursor for the production of several physiologically important steroids that include bile acids, steroid hormones and vitamin D [
7,
29,
30]. Following, an increase in cholesterol, it may lead to increased testosterone concentration via steroidogenesis which should normally reflect in a corresponding increase in libido [
7].
Again, Local herbs have resulted in new breakthroughs in the treatment of sexual dysfunction and have become recognized worldwide as an immediate therapy [
31]. Using plants as aphrodisiac in treating sexual disorders is gaining ground every day. Many of the effective herbal aphrodisiacs are accessible and have slight or no side effects [
32]. Although, there are conventional treatment options but they have limited efficacy, hostile side effects and contraindications in some disease states [
11]. The side effects of sildenafil citrate (Viagra)-a popular aphrodisiac drug include rashes, hypotension, facial flushing and urinary tract infection. Other side effects include back pain, nasal congestion, blurred vision, stomach upset, suicidal tendencies, mental disorders and dilation of the blood vessels [
33,
34]. Other management therapies include surgery, psychiatric therapy, vacuum devices, penile implants and very expensive drugs which may not be affordable [
35].
Aloe barbadensis (AB) is a short stemmed succulent herb in the Asphodelaceae family that have been acclaimed by locals in Nigeria to have libido enhancing property [
36]. It has been refer to as being used in herbal preparations since the beginning of the first century AD. The extracts are said to have a rejuvenating, healing and soothing property as marketed in the cosmetic and alternative medicine industries [
37,
38]. The leaf juice is used to treat intestinal ulcer and gynaecological problems and to treat catarrh [
38]. It can be passed over flame and used to clear skin irritation such as ringworm and eczema. The root of
A. barbadensis is used to cure constipation and impotency [
39]. The fresh leaf juice is taken orally to treat stomach ulcer and externally to heal wounds [
40]. It is used as a purgative, appetite-stimulant, emmenogogue and for managing colds, piles, asthma, cough and jaundice in ayurvedic formulation [
41]. Previous reports show that the extract of AB have been proven via in vivo and in vitro studies to have effects on reproductive functions. Oyewopo et al. [
42] observed that the extract had profound effect on the testicular weight and semen parameters of Sprague-Dawley rats. Iwu [
43] also reported that 60 mg/kg b.w. of AB powder potentiated the rate of fertility and litter size of rabbits. However, from unpublished data (Erhabor, Obarisiagbon and Gabriel) a significant dose dependence increase (100, 200, 400 and 800 mg/kg) in sperm motility and count without any deleterious effect on the morphology was noticed. In another study by Ahmadi et al. [
44] the extract of AB showed ability to influence reproductive functions in animals.
It is against this background, that this research was targeted at probing the potential activity of the ethanol extract of A. barbadensis root on the sexual behavior of male rats in order to determine the possible mechanism of action of the already implicated bioactives. It was also to corroborate the ethnomedical use of the plant as an aphrodisiac and against other male reproductive dysfunctions.
Methods
Collection and authentication of plant material
The roots of Aloe barbadensis were collected from Okene town in Okene local government area of Kogi State, Nigeria. It was initially identified by the authors (MI and JOE) both from the Department of Plant Biology and Biotechnology, University of Benin, Benin City, and further authenticated by Mr. Ibhanesebhor, G. of the Herbarium Unit of the Obafemi Awolowo University, Ile-Ife, Nigeria, with voucher number IFE17004 were the plant was deposited. The plant was also deposited at the herbarium of the Department of Plant Biology and Biotechnology, University of Benin, Benin City with voucher number UPBHx0160.
The fresh roots of Aloe barbadensis were detached from the whole uprooted plant, rinsed in water and spread on laboratory tables where they were dried under room temperature. The plant material were then transferred to an oven set at 40 °C for 5–10 min before being reduced to fine powder with the aid of a mechanical grinder. 2000 g of the powdered plant material was extracted with 5000 ml of ethanol using a soxhlet extractor. The extract was concentrated to dryness using a water bath (HH-S Water Bath; Searchtech Instruments) set at an average temperature of 50 °C. The percentage yield of the ethanolic extract was determined using the formular (% Yield = weight of extract/weight of powder sample × 100/1).
Drugs, assay kits and other reagents
Estradiol benzoate and progesterone were purchased from Sigma-Aldrich from China and USA. Sildenafil citrate was obtained from a community pharmacy outlet in Benin City, Edo State. The testosterone assay kit was procured from Monobind Inc., USA while every other chemicals used were of analytical grade.
Animal groupings and extract administration
Seventy five (75) male and thirty 30 non oestrus female Wistar rats of 140–270 g and 145–260 g body weights was used for this study. The animals were acquired from the animal house of the Department of Anatomy, Faculty of Basic Medical Sciences, University of Benin, Nigeria. The animals were kept in sanitary wooden cages placed in well aerated animal house of the Department of Animal and Environmental Biology, Faculty of Life Sciences, for acclimatization with peak conditions (temperature, 25 °C; photoperiod, 12 h of natural light and 12 h of dark). The animals were permitted free access to water and fed with normal commercial pellets. The cages were cleaned daily throughout the period of the work. The animals were monitored once daily for their general health and weighed weekly. No adverse events was noticed before and during the experiment. The 75 male rats were wholly randomized into five groups of 15 and given appropriate treatment orally.
Group A was given the diluent (2 ml of distilled water) while group B, C and D was given 100, 200 and 400 mg/kg body weight, respectively, of
Aloe barbadensis root extract. Group E was given the standard drug-Sildenafil citrate (5 mg/kg). The oral administration was carried out using orogastric tube. All animals used in this study were handled following the international guiding principles for biomedical research involving animals as outlined by the Council for International Organization of Medical Sciences and the International Council for Laboratory Animal Science [
45].
Mating Behavioural study
The mating behavioural tests was done following the modification of the procedures of [
46‐
48]. From the groups (in a completely randomized manner), five male rats each were selected and observed for sexual behaviour after their diurnal doses on day 1, 7 and 14. The test was done between 19.00 and 03.00 h under a faint light in the Laboratory. The female animals were artificially brought into oestrus (heat) by the successive administration of estradiol benzoate (10 μg/100 g body weight) and progesterone (0.5 mg/100 g body weight) through subcutaneous injections, 48 h and 4 h respectively prior to pairing. This was done because the female rats only allow mating during the oestrus phase. The receptive female rats were introduced to the male rats, 30 min after administration of the extract at the respective doses to the male rats in a locally manufactured wooden cage with glass doors. The female rats were paired with the male rats in all the various doses including controls in the ratio 1:1 (1 female to 1 male). The observation for mating behaviour commenced after 10 min of placing the paired animals in the cage and was recorded with the aid of a video camera on a tripod stand. The test was discontinued if the male fail to manifest sexual interest. Any female animal that do not show receptivity was replaced by another artificially ‘heated’ female.
The occurrence of events and phases of mating after the video recording were analyzed and the frequencies and phases determined. The parameters of male sexual behavior as defined by [
49] that were monitored after 35 min observation period includes: “Mount (MF) and Intromission frequency (IF) - the number of mounts and intromissions from the time of introduction of the female until ejaculation), Mount (ML) and Intromission latency (IL) - the time interval between the introduction of the female and the first mount or intromission by the male and Ejaculation latency (EL) - the time interval between the first intromission and ejaculation”.
Test for libido
The level of sexual desire of the male rats was assessed by the protocol outlined in [
12]. The libido test was carried out using the mounting and intromission frequencies as well their latencies of the mating behavioural test during the 1st, 7th and 14th day.
Serum preparation
The modified technique as defined by Yakubu et al. [
7] was used in the serum preparation. Blood was collected 1¼ hours after giving the extract, the standard drug (Viagra) and distilled water on day 1, 7 and 14. Under chloroform anesthesia, with the aid of a sterile forcep and scissors the stomach was cut open to expose the internal organs. The blood was collected via cardiac puncture using a 5 ml syringe and needle per animal into the appropriately labeled clean lithium heparin (to collect plasma) and non-coagulant(plain) (to collect serum) sample bottles. The sample bottles were kept at a temperature between 23 and 25 °C for ten minutes to clot. The bottles were centrifuged at 3000 rpm for ten minutes using a laboratory centrifuge. The sera and plasma collected were later aspirated with pasteur pipettes into dry plain bottles and utilized within 12 h of preparation for the testosterone and cholesterol assays.
Determination of serum testosterone
The serum testosterone concentrations was determined quantitatively using the Microplate Enzyme Immunoassay kit for total testosterone concentration in human serum as described in the manufacturer’s test procedure (Accu-Blind ELISA Microwells, Product code: 3725–300, Monobind Inc., USA).
Determination of plasma cholesterol
The method of Röschlau et al. [
50] was used to determine the levels of total cholesterol, using enzymatic kits from Randox Laboratories Limited (LOT NUMBER: 2264CH), United Kingdom.
Acute toxicity study
Twenty-five (25) male rats were utilized in this research and extract given as stated in the mating behavioural study earlier. The animals were completely randomized into five groups of five rats each. In all the groups the animals were monitored for 2 h for any behavioural changes such as hyperactivity, sedation, salivation, diarrhea, accelerated breathing, tail posture and convulsions after administering the extract (at the respective doses-100 mg/kg, 200 mg/kg and 400 mg/kg), distilled water and standard drug (viagra) to the corresponding groups. The mortality or lethality was counted after 24 h and the Lethal Dose (LD50) was determined. All animals were further observed for up to 14 days for any delayed mortality.
Data analysis
Data were presented as mean ± SEM of five replicates. One Way ANOVA was done to compare means of different groups as well as a Duncan multiple range test to analyse differences among different means and the interaction between the variables using SPSS 15.0 computer software package. Differences at P < 0.05 or P < 0.01 were considered statistically significant.