nach oben

Journal of Neuro-Oncology

Erschienen in:

12.07.2016 | Laboratory Investigation

Application of brush cytology for FISH-based detection of 1p/19q codeletion in oligodendroglial tumors

verfasst von: Irena Srebotnik-Kirbiš, Clara Limbäck-Stokin

Erschienen in: Journal of Neuro-Oncology | Ausgabe 3/2016

Einloggen, um Zugang zu erhalten

Abstract

Currently, oligodendroglial tumours (OT) are routinely tested for 1p/19q codeletion, a genetic abnormality of prognostic value. This analysis is most commonly performed by fluorescence in situ hybridization (FISH) on formalin-fixed, paraffin embedded (FFPE) tissue sections, which is time-consuming and often difficult to interpret, mainly due to the presence of truncated and overlapping nuclei. To overcome these methodological disadvantages, we investigated the validity of cytospins prepared from brushings of fresh tissue samples for assessing 1p/19q status by FISH. For this purpose, FISH analysis of 1p/19q codeletion was performed on FFPE tissue sections and cytospins prepared from brushing of corresponding fresh tissue in a series of 35 central nervous system tumours (16 OT and 19 non-OT). An aberrant 1p/19q status was found in 11/16 (69 %) OT samples and included codeletion of 1p/19q (7), 1p/19q imbalance (2) isolated 19q deletion (1) and 1p imbalance (1). None of the 19 non-OT samples showed 1p/19q codeletion. Results of FISH were concordant between FFPE sections and cytospins in all cases in which both types of slides gave interpretable results. Interpretation of FISH signals on cytospins was easier and quicker than on FFPE sections. Our study showed that cytospins prepared from brushing of fresh tissue samples allow quick and reliable FISH based analysis of 1p/19q status and can substitute traditional FFPE sections when fresh tissue is available.

Louis DN, Ohgaki H, Wiestler OD, Cavenee WK, Burger PC, Jouvet A, Scheithauer BW, Kleihues P (2007) The 2007 WHO classification of tumours of the central nervous system. Acta Neuropathol 114(2):97–109. doi:10.1007/s00401-007-0243-4 CrossRefPubMedPubMedCentral

Scheie D, Andresen PA, Cvancarova M, Bo AS, Helseth E, Skullerud K, Beiske K (2006) Fluorescence in situ hybridization (FISH) on touch preparations: a reliable method for detecting loss of heterozygosity at 1p and 19q in oligodendroglial tumors. Am J Surg Pathol 30(7):828–837. doi:10.1097/01.pas.0000213250.44822.2e CrossRefPubMed

Woehrer A, Hainfellner JA (2015) Molecular diagnostics: techniques and recommendations for 1p/19q assessment. CNS Oncol 4(5):295–306. doi:10.2217/cns.15.28 CrossRefPubMed

Aldape K, Burger PC, Perry A (2007) Clinicopathologic aspects of 1p/19q loss and the diagnosis of oligodendroglioma. Arch Pathol Lab Med 131(2):242–251. doi:10.1043/1543-2165(2007)131[242:CAOQLA]2.0.CO;2

Rodriguez FJ, Tihan T, Lin D, McDonald W, Nigro J, Feuerstein B, Jackson S, Cohen K, Burger PC (2014) Clinicopathologic features of pediatric oligodendrogliomas: a series of 50 patients. Am J Surg Pathol 38(8):1058–1070. doi:10.1097/PAS.0000000000000221 PubMedPubMedCentral

Woehrer A, Sander P, Haberler C, Kern S, Maier H, Preusser M, Hartmann C, Kros JM, Hainfellner JA, Research Committee of the European Confederation of Neuropathological S (2011) FISH-based detection of 1p 19q codeletion in oligodendroglial tumors: procedures and protocols for neuropathological practice—a publication under the auspices of the Research Committee of the European Confederation of Neuropathological Societies (Euro-CNS). Clin Neuropathol 30(2):47–55CrossRefPubMed

Berghoff AS, Stefanits H, Woehrer A, Heinzl H, Preusser M, Hainfellner JA, Vienna Comprehensive Cancer Center Central Nervous System U (2013) Clinical neuropathology practice guide 3-2013: levels of evidence and clinical utility of prognostic and predictive candidate brain tumor biomarkers. Clin Neuropathol 32(3):148–158CrossRefPubMedPubMedCentral

Horbinski C, Miller CR, Perry A (2011) Gone FISHing: clinical lessons learned in brain tumor molecular diagnostics over the last decade. Brain Pathol 21(1):57–73. doi:10.1111/j.1750-3639.2010.00453.x CrossRefPubMed

Gonzales M, Dale S, Susman M, Mills J (2006) Quantitation of chromosome 1p and 19q deletions in glial tumours by interphase FISH on formalin-fixed paraffin-embedded tissue. J Clin Neurosci 13(1):96–101. doi:10.1016/j.jocn.2005.06.004 CrossRefPubMed

10.

Louis DN, Perry A, Burger P, Ellison DW, Reifenberger G, von Deimling A, Aldape K, Brat D, Collins VP, Eberhart C, Figarella-Branger D, Fuller GN, Giangaspero F, Giannini C, Hawkins C, Kleihues P, Korshunov A, Kros JM, Beatriz Lopes M, Ng HK, Ohgaki H, Paulus W, Pietsch T, Rosenblum M, Rushing E, Soylemezoglu F, Wiestler O, Wesseling P, International Society Of N-H (2014) International Society of Neuropathology–haarlem consensus guidelines for nervous system tumor classification and grading. Brain Pathol 24(5):429–435. doi:10.1111/bpa.12171 CrossRefPubMed

11.

Sahm F, Reuss D, Koelsche C, Capper D, Schittenhelm J, Heim S, Jones DT, Pfister SM, Herold-Mende C, Wick W, Mueller W, Hartmann C, Paulus W, von Deimling A (2014) Farewell to oligoastrocytoma: in situ molecular genetics favor classification as either oligodendroglioma or astrocytoma. Acta Neuropathol 128(4):551–559. doi:10.1007/s00401-014-1326-7 CrossRefPubMed

12.

Kros JM, Gorlia T, Kouwenhoven MC, Zheng PP, Collins VP, Figarella-Branger D, Giangaspero F, Giannini C, Mokhtari K, Mork SJ, Paetau A, Reifenberger G, van den Bent MJ (2007) Panel review of anaplastic oligodendroglioma from European Organization For Research and Treatment of Cancer Trial 26951: assessment of consensus in diagnosis, influence of 1p/19q loss, and correlations with outcome. J Neuropathol Exp Neurol 66(6):545–551. doi:10.1097/01.jnen.0000263869.84188.72 CrossRefPubMed

13.

Giannini C, Burger PC, Berkey BA, Cairncross JG, Jenkins RB, Mehta M, Curran WJ, Aldape K (2008) Anaplastic oligodendroglial tumors: refining the correlation among histopathology, 1p 19q deletion and clinical outcome in Intergroup Radiation Therapy Oncology Group Trial 9402. Brain Pathol 18(3):360–369. doi:10.1111/j.1750-3639.2008.00129.x CrossRefPubMed

14.

Fallon KB, Palmer CA, Roth KA, Nabors LB, Wang W, Carpenter M, Banerjee R, Forsyth P, Rich K, Perry A (2004) Prognostic value of 1p, 19q, 9p, 10q, and EGFR-FISH analyses in recurrent oligodendrogliomas. J Neuropathol Exp Neurol 63(4):314–322CrossRefPubMed

15.

van den Bent MJ, Brandes AA, Taphoorn MJ, Kros JM, Kouwenhoven MC, Delattre JY, Bernsen HJ, Frenay M, Tijssen CC, Grisold W, Sipos L, Enting RH, French PJ, Dinjens WN, Vecht CJ, Allgeier A, Lacombe D, Gorlia T, Hoang-Xuan K (2013) Adjuvant procarbazine, lomustine, and vincristine chemotherapy in newly diagnosed anaplastic oligodendroglioma: long-term follow-up of EORTC brain tumor group study 26951. J Clin Oncol 31(3):344–350. doi:10.1200/JCO.2012.43.2229 CrossRefPubMed

16.

Cairncross G, Wang M, Shaw E, Jenkins R, Brachman D, Buckner J, Fink K, Souhami L, Laperriere N, Curran W, Mehta M (2013) Phase III trial of chemoradiotherapy for anaplastic oligodendroglioma: long-term results of RTOG 9402. J Clin Oncol 31(3):337–343. doi:10.1200/JCO.2012.43.2674 CrossRefPubMed

17.

Fuller CE, Schmidt RE, Roth KA, Burger PC, Scheithauer BW, Banerjee R, Trinkaus K, Lytle R, Perry A (2003) Clinical utility of fluorescence in situ hybridization (FISH) in morphologically ambiguous gliomas with hybrid oligodendroglial/astrocytic features. J Neuropathol Exp Neurol 62(11):1118–1128CrossRefPubMed

18.

Snuderl M, Eichler AF, Ligon KL, Vu QU, Silver M, Betensky RA, Ligon AH, Wen PY, Louis DN, Iafrate AJ (2009) Polysomy for chromosomes 1 and 19 predicts earlier recurrence in anaplastic oligodendrogliomas with concurrent 1p/19q loss. Clin Cancer Res 15(20):6430–6437. doi:10.1158/1078-0432.CCR-09-0867 CrossRefPubMedPubMedCentral

19.

Chamberlain MC, Born D (2015) Prognostic significance of relative 1p/19q codeletion in oligodendroglial tumors. J Neurooncol 125(2):249–251. doi:10.1007/s11060-015-1906-y CrossRefPubMed

20.

Lass U, Hartmann C, Capper D, Herold-Mende C, von Deimling A, Meiboom M, Mueller W (2013) Chromogenic in situ hybridization is a reliable alternative to fluorescence in situ hybridization for diagnostic testing of 1p and 19q loss in paraffin-embedded gliomas. Brain Pathol 23(3):311–318. doi:10.1111/bpa.12003 CrossRefPubMed

21.

Rossi E, Ubiali A, Balzarini P, Cadei M, Alpi F, Grigolatoi PG (2005) High-level detection of gene amplification and chromosome aneuploidy in extracted nuclei from paraffin-embedded tissue of human cancer using FISH: a new approach for retrospective studies. Eur J Histochem 49(1):53–58CrossRefPubMed

22.

Brunelli M, Segala D, Delahunt B, Parolini C, Bersani S, Cheng L, Eble JN, Chilosi M, Gobbo S, Martignoni G (2011) FISH scoring on paraffin sections versus single-cell suspension for chromophobe renal carcinoma and renal oncocytoma. Anticancer Res 31(10):3137–3142PubMed

23.

Qian J, Bostwick DG, Takahashi S, Borell TJ, Brown JA, Lieber MM, Jenkins RB (1996) Comparison of fluorescence in situ hybridization analysis of isolated nuclei and routine histological sections from paraffin-embedded prostatic adenocarcinoma specimens. Am J Pathol 149(4):1193–1199PubMedPubMedCentral

24.

Schurter MJ, LeBrun DP, Harrison KJ (2002) Improved technique for fluorescence in situ hybridisation analysis of isolated nuclei from archival, B5 or formalin fixed, paraffin wax embedded tissue. Mol Pathol 55(2):121–124CrossRefPubMedPubMedCentral

25.

Gelpi E, Ambros IM, Birner P, Luegmayr A, Drlicek M, Fischer I, Kleinert R, Maier H, Huemer M, Gatterbauer B, Anton J, Rossler K, Budka H, Ambros PF, Hainfellner JA (2003) Fluorescent in situ hybridization on isolated tumor cell nuclei: a sensitive method for 1p and 19q deletion analysis in paraffin-embedded oligodendroglial tumor specimens. Mod Pathol 16(7):708–715. doi:10.1097/01.MP.0000076981.90281.BF CrossRefPubMed

26.

Bouvier C, Roll P, Quilichini B, Metellus P, Calisti A, Gilles S, Chinot O, Fina F, Martin PM, Figarella-Branger D (2004) Deletions of chromosomes 1p and 19q are detectable on frozen smears of gliomas by FISH: usefulness for stereotactic biopsies. J Neurooncol 68(2):141–149CrossRefPubMed

27.

Belaud-Rotureau MA, Meunier N, Eimer S, Vital A, Loiseau H, Merlio JP (2006) Automatized assessment of 1p36-19q13 status in gliomas by interphase FISH assay on touch imprints of frozen tumours. Acta Neuropathol 111(3):255–263. doi:10.1007/s00401-005-0001-4 CrossRefPubMed

28.

Navaneethan U, Njei B, Lourdusamy V, Konjeti R, Vargo JJ, Parsi MA (2015) Comparative effectiveness of biliary brush cytology and intraductal biopsy for detection of malignant biliary strictures: a systematic review and meta-analysis. Gastrointest Endosc 81(1):168–176. doi:10.1016/j.gie.2014.09.017 CrossRefPubMed

29.

Stewart CJ, Mills PR, Carter R, O’Donohue J, Fullarton G, Imrie CW, Murray WR (2001) Brush cytology in the assessment of pancreatico-biliary strictures: a review of 406 cases. J Clin Pathol 54(6):449–455CrossRefPubMedPubMedCentral

30.

Michels G, Topalidis T, Buttner R, Engels M, Pfister R (2012) Usefulness of imprint and brushing cytology in diagnosis of lung diseases with flexible bronchoscopy. J Clin Pathol 65(7):649–653. doi:10.1136/jclinpath-2012-200707 CrossRefPubMed

31.

Babshet M, Nandimath K, Pervatikar S, Naikmasur V (2011) Efficacy of oral brush cytology in the evaluation of the oral premalignant and malignant lesions. J Cytol 28(4):165–172. doi:10.4103/0970-9371.86342 CrossRefPubMedPubMedCentral

32.

Layfield LJ, Ehya H, Filie AC, Hruban RH, Jhala N, Joseph L, Vielh P, Pitman MB (2014) Utilization of ancillary studies in the cytologic diagnosis of biliary and pancreatic lesions: the Papanicolaou society of cytopathology guidelines. Cytojournal 11(Suppl 1):4. doi:10.4103/1742-6413.133352 PubMedPubMedCentral

33.

Srebotnik-Kirbiš I, Us Krašovec M, Pogačnik A, Strojan Fležar M (2015) Optimization and validation of immunocytochemical detection of estrogen receptors on cytospins prepared from FNA samples of breast cancer. Cytopathology 26:88–98CrossRefPubMed

34.

Ambros PF, Ambros IM, Siop Europe Neuroblastoma Pathology B, Bone Marrow G (2001) Pathology and biology guidelines for resectable and unresectable neuroblastic tumors and bone marrow examination guidelines. Med Pediatr Oncol 37(6):492–504CrossRefPubMed

35.

Senetta R, Verdun di Cantogno L, Chiusa L, Castellano I, Gugliotta P, Sapino A, Cassoni P (2013) A “weighted” fluorescence in situ hybridization strengthens the favorable prognostic value of 1p/19q codeletion in pure and mixed oligodendroglial tumors. J Neuropathol Exp Neurol 72(5):432–441. doi:10.1097/NEN.0b013e3182901f41 CrossRefPubMedPubMedCentral

36.

Nagasaka T, Gunji M, Hosokai N, Hayashi K, Fujino M, Ikeda H, Ito M, Inao S (2010) Fluorescent in situ hybridization 1p/19q deletion/imbalance analysis of low-grade and atypical meningiomas. Neurol Med Chir (Tokyo) 50(1):27–32 (discussion 32)CrossRef

37.

Ketter R, Rahnenfuhrer J, Henn W, Kim YJ, Feiden W, Steudel WI, Zang KD, Urbschat S (2008) Correspondence of tumor localization with tumor recurrence and cytogenetic progression in meningiomas. Neurosurgery 62(1):61–69 (discussion 69–70). doi:10.1227/01.NEU.0000311062.72626.D6 CrossRefPubMed

38.

Reuss DE, Sahm F, Schrimpf D, Wiestler B, Capper D, Koelsche C, Schweizer L, Korshunov A, Jones DT, Hovestadt V, Mittelbronn M, Schittenhelm J, Herold-Mende C, Unterberg A, Platten M, Weller M, Wick W, Pfister SM, von Deimling A (2015) ATRX and IDH1-R132H immunohistochemistry with subsequent copy number analysis and IDH sequencing as a basis for an “integrated” diagnostic approach for adult astrocytoma, oligodendroglioma and glioblastoma. Acta Neuropathol 129(1):133–146. doi:10.1007/s00401-014-1370-3 CrossRefPubMed

39.

Olar A, Wani KM, Alfaro-Munoz KD, Heathcock LE, van Thuijl HF, Gilbert MR, Armstrong TS, Sulman EP, Cahill DP, Vera-Bolanos E, Yuan Y, Reijneveld JC, Ylstra B, Wesseling P, Aldape KD (2015) IDH mutation status and role of WHO grade and mitotic index in overall survival in grade II-III diffuse gliomas. Acta Neuropathol 129(4):585–596. doi:10.1007/s00401-015-1398-z CrossRefPubMedPubMedCentral

40.

Duffy L, Zhang L, Love DR, George AM (2012) Quality control considerations for fluorescence in situ hybridisation of paraffin-embedded pathology specimens in a diagnostic laboratory environment. In: Akyar I (ed) Latest research into quality control. InTech

Titel: Application of brush cytology for FISH-based detection of 1p/19q codeletion in oligodendroglial tumors
verfasst von: Irena Srebotnik-Kirbiš
Clara Limbäck-Stokin
Publikationsdatum: 12.07.2016
Verlag: Springer US
Erschienen in: Journal of Neuro-Oncology / Ausgabe 3/2016
Print ISSN: 0167-594X
Elektronische ISSN: 1573-7373
DOI: https://doi.org/10.1007/s11060-016-2211-0

Leitlinien kompakt für die Neurologie

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Neu im Fachgebiet Neurologie

16.04.2024 | Maligne primäre ZNS-Tumoren | Nachrichten

Update Neurologie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Springer Medizin

Application of brush cytology for FISH-based detection of 1p/19q codeletion in oligodendroglial tumors

Abstract

Leitlinien kompakt für die Neurologie

Neu im Fachgebiet Neurologie

Manche Gestagene können das Risiko für Meningeome erhöhen

Parkinson: Größere Studie bestätigt Genauigkeit von Hauttest

Simple Kortisontherapie stoppt primär progrediente Aphasie

Impostor-Syndrom häufig bei Hyperhidrose zu finden

Update Neurologie

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 3/2016

Rapid progression of intracranial melanoma metastases controlled with combined BRAF/MEK inhibition after discontinuation of therapy: a clinical challenge

Age alone is not a predictor for survival in glioblastoma

The effects of sequential treatments on hippocampal volumes in malignant glioma patients

Impact of tapering and discontinuation of bevacizumab in patients with progressive glioblastoma

Low expressions of ASS1 and OTC in glioblastoma suggest the potential clinical use of recombinant human arginase (rhArg)

Pembrolizumab: first experience with recurrent primary central nervous system (CNS) tumors

Leitlinien kompakt für die Neurologie

Neu im Fachgebiet Neurologie

Manche Gestagene können das Risiko für Meningeome erhöhen

Parkinson: Größere Studie bestätigt Genauigkeit von Hauttest

Simple Kortisontherapie stoppt primär progrediente Aphasie

Impostor-Syndrom häufig bei Hyperhidrose zu finden

Update Neurologie