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01.12.2018 | Methodology | Ausgabe 1/2018 Open Access

Malaria Journal 1/2018

Application of the automated haematology analyzer XN-30 in an experimental rodent model of malaria

Zeitschrift:
Malaria Journal > Ausgabe 1/2018
Autoren:
Takahiro Tougan, Yuhgi Suzuki, Munehisa Izuka, Kei Aono, Tomonori Okazaki, Yuji Toya, Kinya Uchihashi, Toshihiro Horii
Wichtige Hinweise

Electronic supplementary material

The online version of this article (https://​doi.​org/​10.​1186/​s12936-018-2313-6) contains supplementary material, which is available to authorized users.

Abstract

Background

The erythrocytic stage, where malaria parasites proliferate in human blood, is clinically significant as this causes the symptoms and illness of malaria. Experimental rodent models of malaria at the erythrocytic stage are used for the development of anti-malarial drugs and for biological analysis. An automated haematology analyzer XN-30 was developed for detection of infected red blood cells (iRBCs) in human blood samples and measurement of their parasitaemia in approximately 1 min through flow cytometry analysis. Additionally, the analyzer simultaneously measured other haematological parameters in these samples. It is inferred that the analyzer would also allow easy and rapid measurement of parasitaemia in mice and provide important clues on the mouse haematological state during infection and treatment.

Results

The XN-30 analyzer is a simple and rapid tool to detect iRBCs in mouse blood samples infected with rodent malarial parasites, with three-dimensional analysis permitting the precise measurement of parasitaemia (referred herein as the ‘XN-30 system’). The XN-30 analyzer allowed not only the detection of iRBCs but also the monitoring of RBC, white blood cell, and platelet counts, as well as haematocrit, mean corpuscular volume and mean platelet volume values in the mouse blood sample. For anti-malarial drug development, aside from demonstrating possible efficacy in mouse models, XN-30 analyzer could provide a first glimpse of the safety profile of the drug.

Conclusions

The XN-30 system is a powerful tool that can be utilized for the in vivo screening, development, and evaluation of anti-malarial drugs as well as for pre-clinical pharmacology and/or toxicity tests in rodent models.
Zusatzmaterial
Additional file 1: Figure S1. Analysis of data obtained using the XN-30 analyzer on blood samples from healthy non-infected mice, related to Fig. 1. Figure S2. M scattergrams re-analysed using the Flowing software after parasite infection. Figure S3. Comparison between parasitaemias determined using the XN-30 system and microscopy. Figure S4. Morphological evidence of the high MCV and MPV values in infected blood samples by microscopy. Figure S5. M scattergrams re-analysed using the Flowing software after treatment with artemisinin. Figure S6. Re-analysed scattergrams of mouse blood samples infected with parasites.
Additional file 2: Table S1. Data of false-positive iRBCs and HJB-RBCs in non-infected mice. Table S2. Comparison of parasitaemias obtained using the XN-30 system and microscopy, related to Fig. 3. Table S3. Comparison of parasitaemia, related to Fig. 4a. Table S4. Sequential analysis of RBC count, related to Fig. 4b(i). Table S5. Sequential analysis of WBC count, related to Fig. 4b(ii). Table S6. Sequential analysis of PLT value count, related to Fig. 4b(iii). Table S7. Sequential analysis of HCT value, related to Fig. 4b(iv). Table S8. Sequential analysis of MCV value, related to Fig. 4b(v). Table S9. Sequential analysis of MPV value, related to Fig. 4b(vi). Table S10. Sequential analysis of parasitaemia and the parameters after treatment with artemisinin, related to Fig. 5.
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