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Erschienen in: Pituitary 1/2008

01.03.2008

Aromatase cytochrome P450 enzyme expression in human pituitary

verfasst von: Pinar Kadioglu, Gokhan Oral, Muge Sayitoglu, Nevin Erensoy, Berna Senel, Nurperi Gazioglu, Aydin Sav, Gursel Cetin, Ugur Ozbek

Erschienen in: Pituitary | Ausgabe 1/2008

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Abstract

Aromatase (P450AROM) converts testosterone to estrogen. This conversion could be important in normal physiology and estradiol-induced tumorigenesis in human pituitary. The objective of this study was to examine the expression of P450AROM in normal human pituitary and determine the gender difference. We examined aromatase expression in 19 normal human pituitary glands [13 males, 6 females, median age: 30 years (interquartile ranges, IQR: 23–63)] obtained from autopsy. We demonstrated aromatase gene expression levels by quantitative RT-PCR and aromatase protein with immunohistochemical staining in normal male and female human pituitary. Although median relative expression level of aromatase mRNA of male individuals [median ΔCt = 42.6 (IQR: 7.6–93.9)] was higher than the female individuals [median ΔCt = 3.9 (IQR:0–44.8)], we could not determine a significant gender difference in aromatase mRNA levels (p = 0.2). The difference between the aromatase protein density by immunohistochemistry was not significant between genders (p = 0.78). The aromatase levels were also not correlated with the age of the study subjects (p = 0.42 r = −0.21). The results indicate that aromatase enzyme is present in human pituitary. The amount and the density of the enzyme show a large variance among different individuals. Although higher mRNA expression was observed in male pituitary compared to female pituitary, there was no statistically significant difference for gender or age.
Literatur
1.
Zurück zum Zitat Harada N (1988) Cloning of a complete cDNA encoding human aromatase: Immunochemical identification and sequence analysis. Biochem Biophys Res Commun 156:725–732PubMedCrossRef Harada N (1988) Cloning of a complete cDNA encoding human aromatase: Immunochemical identification and sequence analysis. Biochem Biophys Res Commun 156:725–732PubMedCrossRef
2.
Zurück zum Zitat Bagatell CJ, Dahl KD, Bremner WJ (1994) The direct pituitary effect of testosterone to inhibit gonadotropin secretion in men is partially mediated by aromatization to estradiol. J Androl 15:15–21PubMed Bagatell CJ, Dahl KD, Bremner WJ (1994) The direct pituitary effect of testosterone to inhibit gonadotropin secretion in men is partially mediated by aromatization to estradiol. J Androl 15:15–21PubMed
3.
Zurück zum Zitat Means GD, Mahendroo MS, Corbin CJ et al (1989) Structural analysis of the gene encoding human aromatase cytochrome P-450, the enzyme responsible for estrogen biosynthesis. J Biol Chem 264:19385–19391PubMed Means GD, Mahendroo MS, Corbin CJ et al (1989) Structural analysis of the gene encoding human aromatase cytochrome P-450, the enzyme responsible for estrogen biosynthesis. J Biol Chem 264:19385–19391PubMed
4.
Zurück zum Zitat Sheckter CB, Matsumoto AM, Bremner WJ (1989) Testosterone administration inhibits gonadotropin secretion by an effect directly on the human pituitary. J Clin Endocrinol Metab 68:397–401PubMed Sheckter CB, Matsumoto AM, Bremner WJ (1989) Testosterone administration inhibits gonadotropin secretion by an effect directly on the human pituitary. J Clin Endocrinol Metab 68:397–401PubMed
5.
Zurück zum Zitat Abdelgadir SE, Resko JA, Ojeda SR, Lephart ED, McPhaul MJ, Roselli CE (1994) Androgen regulate aromatase cytochrome P450 messenger ribonucleic acid in rat brain. Endocrinology 135:395–401PubMedCrossRef Abdelgadir SE, Resko JA, Ojeda SR, Lephart ED, McPhaul MJ, Roselli CE (1994) Androgen regulate aromatase cytochrome P450 messenger ribonucleic acid in rat brain. Endocrinology 135:395–401PubMedCrossRef
6.
Zurück zum Zitat Brueggemeier RW, Hackett JC, Diaz-Cruz ES (2005) Aromatase inhibitors in the treatment of breast cancer. Endocr Rev 26:331–345PubMedCrossRef Brueggemeier RW, Hackett JC, Diaz-Cruz ES (2005) Aromatase inhibitors in the treatment of breast cancer. Endocr Rev 26:331–345PubMedCrossRef
7.
Zurück zum Zitat Sasano H, Takahashi K, Satoh F, Nagura H, Harada N (1998) Aromatase in the human central nervous system. Clin Endocrinol (Oxf) 48:325–329CrossRef Sasano H, Takahashi K, Satoh F, Nagura H, Harada N (1998) Aromatase in the human central nervous system. Clin Endocrinol (Oxf) 48:325–329CrossRef
8.
Zurück zum Zitat Xu RK, Wu XM, Di AK, Xu JN, Pang CS, Pang SF (2000) Pituitary prolactin-secreting tumor formation: recent developments. Biol Signals Recept 9:1–20PubMedCrossRef Xu RK, Wu XM, Di AK, Xu JN, Pang CS, Pang SF (2000) Pituitary prolactin-secreting tumor formation: recent developments. Biol Signals Recept 9:1–20PubMedCrossRef
9.
Zurück zum Zitat Spady TJ, Harvell DME, Snyder MC, Pennington KL, McComb RD, Shull JD (1998) Estrogen-induced tumorigenesis in the Copenhagen rat: disparate susceptibilies to development of prolactin-producing pituitary tumors and mammary carcinomas. Cancer Lett 124:95–103PubMedCrossRef Spady TJ, Harvell DME, Snyder MC, Pennington KL, McComb RD, Shull JD (1998) Estrogen-induced tumorigenesis in the Copenhagen rat: disparate susceptibilies to development of prolactin-producing pituitary tumors and mammary carcinomas. Cancer Lett 124:95–103PubMedCrossRef
10.
Zurück zum Zitat Gottesfeld Z, Liehr JG (1987) Chronic exposure to random restraint stress retards the development of estrogen-induced pituitary prolactinoma in rats. Neurosci Lett 80:44–48PubMedCrossRef Gottesfeld Z, Liehr JG (1987) Chronic exposure to random restraint stress retards the development of estrogen-induced pituitary prolactinoma in rats. Neurosci Lett 80:44–48PubMedCrossRef
11.
Zurück zum Zitat Prager D, Braunstein GD (1995) Pituitary disorders during pregnancy. Endocrinol Metab Clin North Am 24:1–14PubMed Prager D, Braunstein GD (1995) Pituitary disorders during pregnancy. Endocrinol Metab Clin North Am 24:1–14PubMed
12.
Zurück zum Zitat Gooren LJ, Assies J, Asscheman H, de Slegte R, van Kessel H (1988) Estrogen-induced prolactinoma in a man. J Clin Endocrinol Metab 66:444–446PubMed Gooren LJ, Assies J, Asscheman H, de Slegte R, van Kessel H (1988) Estrogen-induced prolactinoma in a man. J Clin Endocrinol Metab 66:444–446PubMed
13.
Zurück zum Zitat Prior JC, Cox TA, Fairholm D, Kosthashuk E, Nugent R (1987) Testosterone-related exacerbation of a prolactin-producing macroadenoma: possible role for estrogen. J Clin Endocrinol Metab 64:391–394PubMed Prior JC, Cox TA, Fairholm D, Kosthashuk E, Nugent R (1987) Testosterone-related exacerbation of a prolactin-producing macroadenoma: possible role for estrogen. J Clin Endocrinol Metab 64:391–394PubMed
14.
Zurück zum Zitat Sodi R, Fikri R, Diver M, Ranganath L, Vora J (2005) Testosterone replacement-induced hyperprolactinaemia: case report and review of the literature. Ann Clin Biochem 42(Pt 2):153–159PubMedCrossRef Sodi R, Fikri R, Diver M, Ranganath L, Vora J (2005) Testosterone replacement-induced hyperprolactinaemia: case report and review of the literature. Ann Clin Biochem 42(Pt 2):153–159PubMedCrossRef
15.
Zurück zum Zitat Gillam MP, Middler S, Freed DJ, Molitch M (2002) The novel use of very high doses of cabergoline and a combination of testosterone and an aromatase inhibitor in the treatment of a giant prolactinoma. J Clin Endocrinol Metab 87:4447–4451PubMedCrossRef Gillam MP, Middler S, Freed DJ, Molitch M (2002) The novel use of very high doses of cabergoline and a combination of testosterone and an aromatase inhibitor in the treatment of a giant prolactinoma. J Clin Endocrinol Metab 87:4447–4451PubMedCrossRef
16.
Zurück zum Zitat Naftolin F, Ryan KJ, Davies IJ et al (1975) The formation of estrogen by central neuroendocrine tissues. Recent Prog Horm Res 31:295–319PubMed Naftolin F, Ryan KJ, Davies IJ et al (1975) The formation of estrogen by central neuroendocrine tissues. Recent Prog Horm Res 31:295–319PubMed
17.
Zurück zum Zitat Flores F, Naftolin F, Ryan KJ, White RJ (1973) Estrogen formation by the isolated perfused Rhesus monkey brain. Science 180:1074–1075PubMedCrossRef Flores F, Naftolin F, Ryan KJ, White RJ (1973) Estrogen formation by the isolated perfused Rhesus monkey brain. Science 180:1074–1075PubMedCrossRef
18.
Zurück zum Zitat Lieberburg I, McEwen BS (1977) Brain cell nuclear retention of metabolites, 5α dihydrotestosterone and estrodiol-17β, in adult rats. Endocrinology 100:588–597PubMed Lieberburg I, McEwen BS (1977) Brain cell nuclear retention of metabolites, 5α dihydrotestosterone and estrodiol-17β, in adult rats. Endocrinology 100:588–597PubMed
19.
Zurück zum Zitat Carretero J, Vázquez G, Blanco E et al (1999) Immunohistochemical evidence of the presence of aromatase P450 in the rat hypophysis. Cell Tissue Res 295:419–423PubMedCrossRef Carretero J, Vázquez G, Blanco E et al (1999) Immunohistochemical evidence of the presence of aromatase P450 in the rat hypophysis. Cell Tissue Res 295:419–423PubMedCrossRef
20.
Zurück zum Zitat Carretero J, Vazques G, Rubio M et al (2003) Postnatal differentiation of the immunohistochemical expression of aromatase P450 in the rat pituitary gland. Histol Histopathol 18:419–423PubMed Carretero J, Vazques G, Rubio M et al (2003) Postnatal differentiation of the immunohistochemical expression of aromatase P450 in the rat pituitary gland. Histol Histopathol 18:419–423PubMed
21.
Zurück zum Zitat Jakap RL, Horvath TL, Leranth C, Harada N, Naftolin F (1993) Aromatase immunoreactivity in the rat brain: gonadectomy-sensitive hypothalamic neurons and an unresponsive “limbic ring” of the lateral septum-bed nucleus-amgdala complex. Steroid Biochem Mol Biol 44:481–498CrossRef Jakap RL, Horvath TL, Leranth C, Harada N, Naftolin F (1993) Aromatase immunoreactivity in the rat brain: gonadectomy-sensitive hypothalamic neurons and an unresponsive “limbic ring” of the lateral septum-bed nucleus-amgdala complex. Steroid Biochem Mol Biol 44:481–498CrossRef
22.
Zurück zum Zitat Carretero J, Burks DJ, Vazquez G et al (2002) Expression of aromatase P450 is increased in spontaneous prolactinomas of aged rats. Pituitary 5:5–10PubMedCrossRef Carretero J, Burks DJ, Vazquez G et al (2002) Expression of aromatase P450 is increased in spontaneous prolactinomas of aged rats. Pituitary 5:5–10PubMedCrossRef
23.
Zurück zum Zitat Miyamoto J, Matsumoto T, Shiina H, Inoue K, Takada I, Ito S, Itoh J, Minematsu T, Sato T, Yanase T, Nawata H, Osamura YR, Kato S (2007) The pituitary function of androgen receptor constitutes a glucocorticoid production circuit. Mol Cell Biol 27:4807–4814PubMedCrossRef Miyamoto J, Matsumoto T, Shiina H, Inoue K, Takada I, Ito S, Itoh J, Minematsu T, Sato T, Yanase T, Nawata H, Osamura YR, Kato S (2007) The pituitary function of androgen receptor constitutes a glucocorticoid production circuit. Mol Cell Biol 27:4807–4814PubMedCrossRef
24.
Zurück zum Zitat West B, Dannies PS (1980) Effects of estrodiol on prolactin production and dihydroergocryptine-induced inhibition of prolactin production in primary cultures of rat pituitary cells. Endocrinology 106:1108–1113PubMed West B, Dannies PS (1980) Effects of estrodiol on prolactin production and dihydroergocryptine-induced inhibition of prolactin production in primary cultures of rat pituitary cells. Endocrinology 106:1108–1113PubMed
25.
Zurück zum Zitat Magrini B, Ebiner JR, Burckhardt P, Felber JP (1976) Study in the relationship between plasma prolactin levels and androgen metabolism. J Clin Endocrinol Metab 43:944–947PubMedCrossRef Magrini B, Ebiner JR, Burckhardt P, Felber JP (1976) Study in the relationship between plasma prolactin levels and androgen metabolism. J Clin Endocrinol Metab 43:944–947PubMedCrossRef
Metadaten
Titel
Aromatase cytochrome P450 enzyme expression in human pituitary
verfasst von
Pinar Kadioglu
Gokhan Oral
Muge Sayitoglu
Nevin Erensoy
Berna Senel
Nurperi Gazioglu
Aydin Sav
Gursel Cetin
Ugur Ozbek
Publikationsdatum
01.03.2008
Verlag
Springer US
Erschienen in
Pituitary / Ausgabe 1/2008
Print ISSN: 1386-341X
Elektronische ISSN: 1573-7403
DOI
https://doi.org/10.1007/s11102-007-0065-3

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