Introduction
Materials and methods
Cell culture
Reagents
Quantitative PCR
Immunoblotting and immunofluorescence
Cell function assays
Metastatic seeding assay
Histopathological analysis
Statistics
Results
Forced expression of ARTN altered the morphology of ER-MC cells in vitro
BT549 | MDA-MB-231 | ||||
---|---|---|---|---|---|
Gene Function | Gene | Fold change | p-value | Fold change | p-value |
Epithelial
|
CTNNB1
| 0.48 | 1.60E-03 | 0.47 | 1.25E-03 |
CTNNA1
| 1.3 | 8.71E-03 | 0.68 | 5.80E-04 | |
CTNNG
| 0.52 | 8.23E-03 | 0.46 | 1.58E-02 | |
OCLN
| 0.35 | 9.71E-04 | 0.94 | 2.07E-02 | |
CDH1
| 0.12 | 4.93E-02 | 0.21 | 4.94E-03 | |
Mesenchymal
|
SNAI2
| 1.68 | 6.26E-03 | 1.1 | 1.67E-02 |
VIM
| 2.44 | 9.39E-04 | 1.23 | 6.79E-03 | |
TWIST1
| 5.49 | 3.98E-03 | 4.56 | 3.99E-03 | |
SPARC
| 1.82 | 2.03E-02 | 2.35 | 6.39E-03 | |
Metastatic
|
NME1
| 2.7 | 1.57E-02 | 1.09 | 1.53E-03 |
PLAU
| 1.02 | 4.40E-04 | 0.85 | 1.57E-03 | |
MMP9
| 2.11 | 3.64E-02 | 0.49 | 1.44E-03 | |
MET
| 0.85 | 1.70E-02 | 0.76 | 3.10E-03 | |
MMP2
| 1.04 | 2.07E-02 | 4.17 | 1.54E-03 | |
MTA2
| 3.02 | 8.75E-03 | 1.58 | 1.49E-02 | |
MMP1
| 1.29 | 4.36E-03 | 0.58 | 2.18E-02 | |
NFKB1A
| 2.23 | 7.19E-03 | 1.02 | 3.49E-03 | |
NFKB1
| 2.77 | 1.73E-02 | 1.7 | 3.96E-03 | |
ERBB2
| 1.24 | 1.71E-03 | 0.96 | 5.05E-03 | |
MYC
| 3.55 | 3.97E-04 | 0.95 | 1.40E-03 |
Depletion of endogenous ARTN significantly alters the morphology of ER-MC cells in vitro
ARTN stimulates the oncogenicity and invasion of ER-MC cells in vitro
ARTN enhances metastatic seeding of ER- MC cells in vivo
Expression and correlation of endogenous ARTN and TWIST1 in different mammary carcinoma cell lines
Co-expression of ARTN and TWIST1 in ER-MC predicts survival outcome
TWIST1 expression | |||
---|---|---|---|
Low | High | ||
ARTN expression | Negative | 14 (40%) | 21 (60%) |
Weak | 9 (31%) | 20 (69%) | |
Moderate | 3 (20%) | 12 (80%) | |
Strong | 2 (13.3%) | 13 (86.7%) |
RFS | OS | |||
---|---|---|---|---|
Odds ratio (95% CI) |
P
| Odds ratio (95% CI) |
P
| |
ARTN-/ARTN+
| 1.157 (0.971-1.461) | 0.22 | 1.196 (0.943-1.518) | 0.14 |
TWIST1-/TWIST1+
| 1.733 (1.076-2.791) |
0.024
| 1.656 (1.026-2.674) |
0.039
|
Low ARTN-Low TWIST1/High ARTN-Low TWIST1
| 3.420 (0.542-92.950) |
0.029
| 3.676 (0.556-99.842) |
0.024
|
Low ARTN-Low TWIST1/High ARTN-High TWIST1
| 9.202 (0.517-163.761) |
0.014
| 9.268 (0.519-165.511) |
0.013
|