Erschienen in:
11.10.2018 | ASO Author Reflections
ASO Author Reflections: The Whole Transcriptome Landscape of Circulating Tumor Cells in Nonmetastatic Breast Cancer
verfasst von:
Julie E. Lang, MD, Kirstyn E. Brownson, MD
Erschienen in:
Annals of Surgical Oncology
|
Sonderheft 3/2018
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Excerpt
Circulating tumor cells (CTCs) have been demonstrated to be prognostic in all stages of breast cancer.
1,
2 However, few clinicians have incorporated them into practice, because CTCs did not predict which specific therapy would be effective in treating patients.
3 The Fisher paradigm contends that operable breast cancer is a systemic disease at its inception and that in addition to proper surgical management, optimal systemic therapy is critical to disease survival outcomes.
4 Previous generations of CTC assays were limited by technical factors relating to the rarity of CTCs, cellular marker expression, lack of blood preservation tubes compatible with downstream assays, and the high cost of next-generation sequencing.
5 This study evaluated the hypothesis that it is feasible to isolate and profile CTCs from stage II–III breast cancer patients before receipt of surgery or systemic therapy.
6 We evaluated known and novel biomarkers using RNA sequencing to characterize the whole transcriptome of CTCs, because not all DNA mutations are expressed. …
