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Erschienen in: Clinical Oral Investigations 3/2010

01.06.2010 | Original Article

Assessment of different dyes used in leakage studies

verfasst von: Johannes Mente, Stephan Ferk, Jens Dreyhaupt, Andreas Deckert, Miloš Legner, Hans Joerg Staehle

Erschienen in: Clinical Oral Investigations | Ausgabe 3/2010

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Abstract

The goal of this in vitro study was to identify the most suitable dye for endodontic dye leakage studies, which could be a further step towards standardisation. The root canals of 70 extracted, single-rooted human adult teeth were enlarged to apical size 50 using hand instruments. The teeth were divided into seven groups (n = 10 each), and all root canals were completely filled by injection with one of the following dyes: methylene blue 0.5% and 5%, blue ink, black ink, eosin 5%, basic fuchsine 0.5% and drawing ink. Transverse root sections from the coronal, middle and apical part of the roots were examined, and the percentage of the dentine penetrated by dye was evaluated by software-supported light microscopy. In addition, the range of particle size of drawing ink particles was evaluated. There were conspicuous differences in the relative dye penetration into the root dentine and the penetration behaviour in the different root sections (two-way ANOVA, both p < 0.0001). One dye (drawing ink) penetrated less into the root dentine compared with all the others (p <0.0001). The particle size of this agent (0.1–2 µm) corresponds best with the size range of a representative selection of 21 species of pathogenic endodontic bacteria. Compared to the other dyes tested, drawing ink appears to be superior for use in endodontic dye leakage studies. The penetration behaviour into the root dentine of all the other dyes tested might be one factor that limits the applicability of these dyes in dye leakage studies.
Literatur
1.
Zurück zum Zitat Dugas NN, Lawrence HP, Teplitsky PE, Pharoah MJ, Friedman S (2003) Periapical health and treatment quality assessment of root-filled teeth in two Canadian populations. Int Endod J 36:181–192CrossRefPubMed Dugas NN, Lawrence HP, Teplitsky PE, Pharoah MJ, Friedman S (2003) Periapical health and treatment quality assessment of root-filled teeth in two Canadian populations. Int Endod J 36:181–192CrossRefPubMed
2.
Zurück zum Zitat Kirkevang LL, Orstavik D, Horsted-Bindslev P, Wenzel A (2000) Periapical status and quality of root fillings and coronal restorations in a Danish population. Int Endod J 33:509–515CrossRefPubMed Kirkevang LL, Orstavik D, Horsted-Bindslev P, Wenzel A (2000) Periapical status and quality of root fillings and coronal restorations in a Danish population. Int Endod J 33:509–515CrossRefPubMed
3.
Zurück zum Zitat Sidaravicius B, Aleksejuniene J, Eriksen HM (1999) Endodontic treatment and prevalence of apical periodontitis in an adult population of Vilnius, Lithuania. Endod. dent. traumatol 15:210–215CrossRefPubMed Sidaravicius B, Aleksejuniene J, Eriksen HM (1999) Endodontic treatment and prevalence of apical periodontitis in an adult population of Vilnius, Lithuania. Endod. dent. traumatol 15:210–215CrossRefPubMed
4.
Zurück zum Zitat Tronstad L, Asbjornsen K, Doving L, Pedersen I, Eriksen HM (2000) Influence of coronal restorations on the periapical health of endodontically treated teeth. Endod. dent. traumatol 16:218–221CrossRefPubMed Tronstad L, Asbjornsen K, Doving L, Pedersen I, Eriksen HM (2000) Influence of coronal restorations on the periapical health of endodontically treated teeth. Endod. dent. traumatol 16:218–221CrossRefPubMed
5.
Zurück zum Zitat Ingle JI, Glick DN (1965) Modern endodontic therapy. Endodontics, 1st edn. Lea & Febinger, Philadelphia, pp 54–76 Ingle JI, Glick DN (1965) Modern endodontic therapy. Endodontics, 1st edn. Lea & Febinger, Philadelphia, pp 54–76
6.
Zurück zum Zitat Petersson K, Petersson A, Olsson B, Hakansson J, Wennberg A (1986) Technical quality of root fillings in an adult Swedish population. Endod. dent. traumatol 2:99–102CrossRefPubMed Petersson K, Petersson A, Olsson B, Hakansson J, Wennberg A (1986) Technical quality of root fillings in an adult Swedish population. Endod. dent. traumatol 2:99–102CrossRefPubMed
7.
Zurück zum Zitat Bohsali K, Pertot WJ, Hosseini B, Camps J (1998) Sealing ability of super EBA and Dyract as root-end fillings: a study in vitro. Int Endod J 31:338–342CrossRefPubMed Bohsali K, Pertot WJ, Hosseini B, Camps J (1998) Sealing ability of super EBA and Dyract as root-end fillings: a study in vitro. Int Endod J 31:338–342CrossRefPubMed
8.
Zurück zum Zitat Pommel L, Camps J (2001) In vitro apical leakage of system B compared with other filling techniques. J. endod. 27:449–451CrossRefPubMed Pommel L, Camps J (2001) In vitro apical leakage of system B compared with other filling techniques. J. endod. 27:449–451CrossRefPubMed
9.
Zurück zum Zitat Raskin A, D'Hoore W, Gonthier S, Degrange M, Dejou J (2001) Reliability of in vitro microleakage tests: a literature review. J. Adhes. Dent. 3:295–308PubMed Raskin A, D'Hoore W, Gonthier S, Degrange M, Dejou J (2001) Reliability of in vitro microleakage tests: a literature review. J. Adhes. Dent. 3:295–308PubMed
10.
Zurück zum Zitat Tamse A, Katz A, Kablan F (1998) Comparison of apical leakage shown by four different dyes with two evaluating methods. Int Endod J 31:333–337CrossRefPubMed Tamse A, Katz A, Kablan F (1998) Comparison of apical leakage shown by four different dyes with two evaluating methods. Int Endod J 31:333–337CrossRefPubMed
11.
Zurück zum Zitat Wu MK, Wesselink PR (1993) Endodontic leakage studies reconsidered. Part I. Methodology, application and relevance. Int Endod J 26:37–43CrossRefPubMed Wu MK, Wesselink PR (1993) Endodontic leakage studies reconsidered. Part I. Methodology, application and relevance. Int Endod J 26:37–43CrossRefPubMed
12.
Zurück zum Zitat Ahlberg KM, Assavanop P, Tay WM (1995) A comparison of the apical dye penetration patterns shown by methylene blue and india ink in root-filled teeth. Int Endod J 28:30–34CrossRefPubMed Ahlberg KM, Assavanop P, Tay WM (1995) A comparison of the apical dye penetration patterns shown by methylene blue and india ink in root-filled teeth. Int Endod J 28:30–34CrossRefPubMed
13.
Zurück zum Zitat Barthel CR, Moshonov J, Shuping G, Orstavik D (1999) Bacterial leakage versus dye leakage in obturated root canals. Int Endod J 32:370–375CrossRefPubMed Barthel CR, Moshonov J, Shuping G, Orstavik D (1999) Bacterial leakage versus dye leakage in obturated root canals. Int Endod J 32:370–375CrossRefPubMed
14.
Zurück zum Zitat Gilbert SD, Witherspoon DE, Berry CW (2001) Coronal leakage following three obturation techniques. Int Endod J 34:293–299CrossRefPubMed Gilbert SD, Witherspoon DE, Berry CW (2001) Coronal leakage following three obturation techniques. Int Endod J 34:293–299CrossRefPubMed
15.
Zurück zum Zitat Pommel L, Jacquot B, Camps J (2001) Lack of correlation among three methods for evaluation of apical leakage. J. Endod 27:347–350CrossRefPubMed Pommel L, Jacquot B, Camps J (2001) Lack of correlation among three methods for evaluation of apical leakage. J. Endod 27:347–350CrossRefPubMed
16.
Zurück zum Zitat Staab H (1970) Einführung in die theoretische organische Chemie. Weinheim/Weinstraße, Verlag Chemie Staab H (1970) Einführung in die theoretische organische Chemie. Weinheim/Weinstraße, Verlag Chemie
17.
Zurück zum Zitat Susini G, Pommel L, About I, Camps J (2006) Lack of correlation between ex vivo apical dye penetration and presence of apical radiolucencies. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 102:e19–23CrossRefPubMed Susini G, Pommel L, About I, Camps J (2006) Lack of correlation between ex vivo apical dye penetration and presence of apical radiolucencies. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 102:e19–23CrossRefPubMed
18.
Zurück zum Zitat Editorial JOE (2007) Wanted: a base of evidence. J. Endod 33:1401–1402CrossRef Editorial JOE (2007) Wanted: a base of evidence. J. Endod 33:1401–1402CrossRef
19.
Zurück zum Zitat Oliver CM, Abbott PV (2001) Correlation between clinical success and apical dye penetration. Int Endod J 34:637–644CrossRefPubMed Oliver CM, Abbott PV (2001) Correlation between clinical success and apical dye penetration. Int Endod J 34:637–644CrossRefPubMed
20.
Zurück zum Zitat Goldman M, Simmonds S, Rush R (1989) The usefulness of dye-penetration studies reexamined. Oral Surg. Oral med. Oral pathol 67:327–332CrossRefPubMed Goldman M, Simmonds S, Rush R (1989) The usefulness of dye-penetration studies reexamined. Oral Surg. Oral med. Oral pathol 67:327–332CrossRefPubMed
21.
Zurück zum Zitat Johnson WT, Zakariasen KL (1983) Spectrophotometric analysis of microleakage in the fine curved canals found in the mesial roots of mandibular molars. Oral Surg. Oral med. Oral pathol 56:305–309CrossRefPubMed Johnson WT, Zakariasen KL (1983) Spectrophotometric analysis of microleakage in the fine curved canals found in the mesial roots of mandibular molars. Oral Surg. Oral med. Oral pathol 56:305–309CrossRefPubMed
22.
Zurück zum Zitat Kennedy WA, Walker WA 3 rd, Gough RW (1986) Smear layer removal effects on apical leakage. J. Endod. 12:21–27CrossRefPubMed Kennedy WA, Walker WA 3 rd, Gough RW (1986) Smear layer removal effects on apical leakage. J. Endod. 12:21–27CrossRefPubMed
23.
Zurück zum Zitat Pitt Ford TR (1983) Relation between seal of root fillings and tissue response. Oral Surg. Oral Med. Oral Pathol 55:291–294CrossRefPubMed Pitt Ford TR (1983) Relation between seal of root fillings and tissue response. Oral Surg. Oral Med. Oral Pathol 55:291–294CrossRefPubMed
24.
Zurück zum Zitat Gesi A, Hakeberg M, Warfvinge J, Bergenholtz G (2006) Incidence of periapical lesions and clinical symptoms after pulpectomy—a clinical and radiographic evaluation of 1- versus 2-session treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 101:379–388CrossRefPubMed Gesi A, Hakeberg M, Warfvinge J, Bergenholtz G (2006) Incidence of periapical lesions and clinical symptoms after pulpectomy—a clinical and radiographic evaluation of 1- versus 2-session treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 101:379–388CrossRefPubMed
25.
Zurück zum Zitat Marquis VL, Dao T, Farzaneh M, Abitbol S, Friedman S (2006) Treatment outcome in endodontics: the Toronto Study. Phase III: initial treatment. J. Endod. 32:299–306CrossRefPubMed Marquis VL, Dao T, Farzaneh M, Abitbol S, Friedman S (2006) Treatment outcome in endodontics: the Toronto Study. Phase III: initial treatment. J. Endod. 32:299–306CrossRefPubMed
26.
Zurück zum Zitat Orstavik D (1996) Time-course and risk analyses of the development and healing of chronic apical periodontitis in man. Int Endod J 29:150–155CrossRefPubMed Orstavik D (1996) Time-course and risk analyses of the development and healing of chronic apical periodontitis in man. Int Endod J 29:150–155CrossRefPubMed
27.
Zurück zum Zitat Sjogren U, Hagglund B, Sundqvist G, Wing K (1990) Factors affecting the long-term results of endodontic treatment. J. Endod. 16:498–504CrossRefPubMed Sjogren U, Hagglund B, Sundqvist G, Wing K (1990) Factors affecting the long-term results of endodontic treatment. J. Endod. 16:498–504CrossRefPubMed
28.
Zurück zum Zitat Tanomaru Filho M, Figueiredo FA, Tanomaru JM (2005) Effect of different dye solutions on the evaluation of the sealing ability of Mineral Trioxide Aggregate. Pesqui Odontol Bras 19:119–122 Tanomaru Filho M, Figueiredo FA, Tanomaru JM (2005) Effect of different dye solutions on the evaluation of the sealing ability of Mineral Trioxide Aggregate. Pesqui Odontol Bras 19:119–122
29.
Zurück zum Zitat Vogt BF, Xavier CB, Demarco FF, Padilha MS (2006) Dentin penetrability evaluation of three different dyes in root-end cavities filled with mineral trioxide aggregate (MTA). Braz Oral Res 20:132–136CrossRefPubMed Vogt BF, Xavier CB, Demarco FF, Padilha MS (2006) Dentin penetrability evaluation of three different dyes in root-end cavities filled with mineral trioxide aggregate (MTA). Braz Oral Res 20:132–136CrossRefPubMed
30.
Zurück zum Zitat Kersten HW, Moorer WR (1989) Particles and molecules in endodontic leakage. Int Endod J 22:118–124CrossRefPubMed Kersten HW, Moorer WR (1989) Particles and molecules in endodontic leakage. Int Endod J 22:118–124CrossRefPubMed
31.
Zurück zum Zitat Michailesco P, Boudeville P (2003) Calibrated latex microspheres percolation: a possible route to model endodontic bacterial leakage. J. Endod. 29:456–462CrossRefPubMed Michailesco P, Boudeville P (2003) Calibrated latex microspheres percolation: a possible route to model endodontic bacterial leakage. J. Endod. 29:456–462CrossRefPubMed
32.
Zurück zum Zitat Baumgartner JC (2004) Microbiologic aspects of endodontic infections. J. Calif. Dent. Assoc. 32:459–468PubMed Baumgartner JC (2004) Microbiologic aspects of endodontic infections. J. Calif. Dent. Assoc. 32:459–468PubMed
33.
Zurück zum Zitat Siqueira JF Jr (2002) Endodontic infections: concepts, paradigms, and perspectives. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 94:281–293CrossRefPubMed Siqueira JF Jr (2002) Endodontic infections: concepts, paradigms, and perspectives. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 94:281–293CrossRefPubMed
34.
35.
Zurück zum Zitat Scott AC, Vire DE, Swanson R (1992) An evaluation of the Thermafil endodontic obturation technique. J. Endod. 18:340–343CrossRefPubMed Scott AC, Vire DE, Swanson R (1992) An evaluation of the Thermafil endodontic obturation technique. J. Endod. 18:340–343CrossRefPubMed
36.
Zurück zum Zitat Youngson CC, Jones JC, Manogue M, Smith IS (1998) In vitro dentinal penetration by tracers used in microleakage studies. Int Endod J 31:90–99CrossRefPubMed Youngson CC, Jones JC, Manogue M, Smith IS (1998) In vitro dentinal penetration by tracers used in microleakage studies. Int Endod J 31:90–99CrossRefPubMed
37.
Zurück zum Zitat Hülsmann M, Heckendorff M, Lennon A (2003) Chelating agents in root canal treatment: mode of action and indications for their use. Int Endod J 36:810–830CrossRefPubMed Hülsmann M, Heckendorff M, Lennon A (2003) Chelating agents in root canal treatment: mode of action and indications for their use. Int Endod J 36:810–830CrossRefPubMed
38.
Zurück zum Zitat Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams S, Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST (1994) Bergey's manual of determinative bacteriology, 9th edn. Williams & Wilkins, Baltimore Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams S, Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST (1994) Bergey's manual of determinative bacteriology, 9th edn. Williams & Wilkins, Baltimore
39.
40.
Zurück zum Zitat Al-Ghamdi A, Wennberg A (1994) Testing of sealing ability of endodontic filling materials. Endod Dent Traumatol 10:249–255CrossRef Al-Ghamdi A, Wennberg A (1994) Testing of sealing ability of endodontic filling materials. Endod Dent Traumatol 10:249–255CrossRef
41.
Zurück zum Zitat Chong BS, Pitt Ford TR, Watson TF, Wilson RF (1995) Sealing ability of potential retrograde root filling materials. Endod Dent Traumatol 11:264–269CrossRefPubMed Chong BS, Pitt Ford TR, Watson TF, Wilson RF (1995) Sealing ability of potential retrograde root filling materials. Endod Dent Traumatol 11:264–269CrossRefPubMed
42.
Zurück zum Zitat Verissimo DM, Vale MS (2006) Methodologies for assessment of apical and coronal leakage of endodontic filling materials: a critical review. J. Oral Sci. 48:93–98CrossRefPubMed Verissimo DM, Vale MS (2006) Methodologies for assessment of apical and coronal leakage of endodontic filling materials: a critical review. J. Oral Sci. 48:93–98CrossRefPubMed
43.
Zurück zum Zitat Oliver CM, Abbott PV (1991) Entrapped air and its effects on dye penetration of voids. Endod Dent Traumatol 7:135–138CrossRefPubMed Oliver CM, Abbott PV (1991) Entrapped air and its effects on dye penetration of voids. Endod Dent Traumatol 7:135–138CrossRefPubMed
44.
Zurück zum Zitat Spangberg LS, Acierno TG, Yongbum Cha B (1989) Influence of entrapped air on the accuracy of leakage studies using dye penetration methods. J. Endod 15:548–551CrossRefPubMed Spangberg LS, Acierno TG, Yongbum Cha B (1989) Influence of entrapped air on the accuracy of leakage studies using dye penetration methods. J. Endod 15:548–551CrossRefPubMed
45.
Zurück zum Zitat Wimonchit S, Timpawat S, Vongsavan N (2002) A comparison of techniques for assessment of coronal dye leakage. J. Endod 28:1–4CrossRefPubMed Wimonchit S, Timpawat S, Vongsavan N (2002) A comparison of techniques for assessment of coronal dye leakage. J. Endod 28:1–4CrossRefPubMed
46.
Zurück zum Zitat Garberoglio R, Brännström M (1976) Scanning electron microscopic investigation of human dentinal tubules. Arch Oral Biol 21:355–362CrossRefPubMed Garberoglio R, Brännström M (1976) Scanning electron microscopic investigation of human dentinal tubules. Arch Oral Biol 21:355–362CrossRefPubMed
47.
Zurück zum Zitat Mjör IA, Sveen OB, Heyeraas KJ (2001) Pulp-dentin biology in restorative dentistry. Part 1: Normal structure and physiology. Quintessence Int 32:427–446PubMed Mjör IA, Sveen OB, Heyeraas KJ (2001) Pulp-dentin biology in restorative dentistry. Part 1: Normal structure and physiology. Quintessence Int 32:427–446PubMed
48.
Zurück zum Zitat Tronstad L (2008) The Endodontium. In: Tronstad L (ed) Clinical endodontics: a textbook, 3rd edn. Thieme, Stuttgart, pp 1–30 Tronstad L (2008) The Endodontium. In: Tronstad L (ed) Clinical endodontics: a textbook, 3rd edn. Thieme, Stuttgart, pp 1–30
49.
Zurück zum Zitat Delivanis PD, Chapman KA (1982) Comparison and reliability of techniques for measuring leakage and marginal penetration. Oral Surg. Oral Med. Oral Pathol. 53:410–416CrossRefPubMed Delivanis PD, Chapman KA (1982) Comparison and reliability of techniques for measuring leakage and marginal penetration. Oral Surg. Oral Med. Oral Pathol. 53:410–416CrossRefPubMed
Metadaten
Titel
Assessment of different dyes used in leakage studies
verfasst von
Johannes Mente
Stephan Ferk
Jens Dreyhaupt
Andreas Deckert
Miloš Legner
Hans Joerg Staehle
Publikationsdatum
01.06.2010
Verlag
Springer-Verlag
Erschienen in
Clinical Oral Investigations / Ausgabe 3/2010
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI
https://doi.org/10.1007/s00784-009-0299-8

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