Background
Methods
Results
G1 (healthy, n = 50) | G2 (obese, n = 35) | G3 (obese with T2DM, n = 35) | p-Value | ||||
---|---|---|---|---|---|---|---|
G1Vs G2 Vs G3 | G2 Vs G1 | G3 Vs G1 | G3 Vs G2 | ||||
Age (years) | 9.31 ± 1.47 | 9 ± 1.77 | 9.59 ± 1.40 | 0.273 | 0.38 | 0.36 | 0.12 |
Gender | |||||||
• Male | 31 (62%) | 27 (77%) | 22 (63%) | 0.294 | 0.16 | 0.78 | 0.29 |
• Female | 19 (38%) | 8 (23%) | 13 (37%) | ||||
BMI percentile | 81.0 ± 1.2 | 96.8 ± 1.6 | 96.7 ± 1.4 | < 0.001 | < 0.001 | < 0.001 | 0.935 |
Glucose (mg/dL) | 96.46 ± 8.78 | 101 ± 15.40 | 116.65 ± 18.76 | 0.006 | 0.08 | 0.004 | 0.04 |
Insulin (μg/mL) | 10.16 ± 1.48 | 10.50 ± 0.69 | 11.20 ± 0.93 | 0.037 | 0.20 | 0.002 | 0.014 |
C-Peptide (ng/mL) | 2.98 ± 0.43 | 3.13 ± 0.21 | 3.21 ± 0.28 | 0.007 | 0.02 | 0.008 | 0.09 |
HbA1c (%) | 4.17 ± 0.21 | 4.85 ± 0.99 | 7.32 ± 2.62 | < 0.001 | < 0.001 | < 0.001 | < 0.001 |
Cholesterol (mg/dL) | 188.79 ± 21.26 | 190.14 ± 26.75 | 196.45 ± 26.49 | 0.34 | 0.79 | 0.14 | 0.32 |
HDLc (mg/dL) | 39.30 ± 1.82 | 36.91 ± 8.12 | 35.61 ± 10.40 | 0.06 | 0.09 | 0.04 | 0.55 |
LDLc (mg/dL) | 127.43 ± 20.12 | 131.39 ± 25.95 | 135.72 ± 22.23 | 0.252 | 0.43 | 0.08 | 0.45 |
TAG (mg/dL) | 110.32 ± 22.43 | 111.17 ± 18.56 | 125.61 ± 20.65 | 0.001 | 0.80 | 0.002 | 0.001 |
miR-486R | 1.52 ± 0.38 | 2.11 ± 0.25 | 2.71 ± 0.29 | 0.002 | 0.003 | < 0.001 | 0.001 |
miR-146bR | 2.42 ± 0.58 | 3.01 ± 0.18 | 3.54 ± 0.24 | < 0.001 | < 0.001 | < 0.001 | < 0.001 |
miR-15bR | 3.82 ± 0.56 | 4.32 ± 0.27 | 5.10 ± 0.36 | < 0.001 | < 0.001 | < 0.001 | < 0.001 |
Betatrophin (ng/mL) | 47.17 ± 6.85 | 54.54 ± 17.67 | 71.68 ± 29.63 | < 0.001 | < 0.001 | < 0.001 | < 0.001 |
SD: standard deviation, T2DM type 2 diabetes mellitus, BMI body mass index, HDLc high density lipoprotein cholesterol, LDLc low density lipoprotein cholesterol, HbA1c glycated hemoglobin, TAC triacylglycerol, microR-486R microRNA 486 relative expression, miR-146bR microRNA 146b relative expression, miR-15bR microRNA 15b relative expression.
Correlations (Table 2, Figs. 1 & 2)
BMI percentile | Cholesterol (mg/dL) | Glucose (mg/dL) | HDLc (mg/dL) | LDLc (mg/L) | HbA1c (%) | TAG (mg/dL) | Insulin (μg/mL) | C-Peptide (ng/mL) | |||
---|---|---|---|---|---|---|---|---|---|---|---|
G1 (healthy, n = 50) | miR-486R | r | 0.282* | 0.205 | 0.323* | 0.078 | 0.167 | 0.271 | 0.190 | −0.293* | − 0.176 |
p | 0.048 | 0.154 | 0.022 | 0.590 | 0.247 | 0.057 | 0.186 | 0.039 | 0.223 | ||
miR-146bR | r | −0.056 | −0.014 | 0.049 | 0.126 | −0.043 | −0.171 | 0.075 | −0.135 | − 0.156 | |
p | 0.701 | 0.921 | 0.736 | 0.383 | 0.765 | 0.235 | 0.604 | 0.351 | 0.279 | ||
miR-15bR | r | 0.254 | 0.088 | 0.130 | 0.104 | 0.067 | 0.377** | 0.073 | −0.450** | −0.235 | |
p | 0.075 | 0.546 | 0.368 | 0.472 | 0.645 | 0.007 | 0.616 | 0.001 | 0.100 | ||
Betatrophin (ng/mL) | r | 0.335* | −0.087 | 0.339* | − 0.196 | − 0.013 | 0.316** | − 0.275 | 0.064 | 0.053 | |
p | 0.017 | 0.548 | 0.016 | 0.172 | 0.930 | 0.049 | 0.063 | 0.661 | 0.713 | ||
G2 (obese, n = 35) | miR-486R | r | 0.335* | 0.073 | 0.691** | −0.510** | 0.238 | 0.785** | −0.022 | − 0.554** | − 0.424* |
p | 0.049 | 0.675 | 0.000 | 0.002 | 0.168 | 0.000 | 0.902 | 0.001 | 0.011 | ||
miR-146bR | r | 0.673* | −0.288 | 0.397* | −0.362* | − 0.154 | 0.493** | − 0.205 | − 0.314** | − 0.332* | |
p | 0.000 | 0.094 | 0.018 | 0.033 | 0.378 | 0.003 | 0.237 | 0.046 | 0.041 | ||
miR-15bR | r | 0.454* | −0.291 | 0.483** | −0.359* | − 0.152 | 0.551** | − 0.249 | − 0.359* | − 0.344* | |
p | 0.018 | 0.090 | 0.003 | 0.034 | 0.383 | 0.001 | 0.150 | 0.034 | 0.043 | ||
Betatrophin (ng/mL) | r | 0.376* | −0.293 | 0.392* | −0.242 | − 0.206 | 0.363* | − 0.146 | − 0.237 | −0.124 | |
p | 0.026 | 0.087 | 0.020 | 0.161 | 0.236 | 0.041 | 0.403 | 0.171 | 0.579 | ||
G3 (obese with T2DM, n = 35) | miR-486R | r | 0.446** | 0.086 | 0.564** | 0.125 | 0.032 | 0.561** | 0.785** | −0.371* | − 0.765** |
p | 0.007 | 0.621 | 0.000 | 0.473 | 0.855 | 0.000 | 0.000 | 0.021 | 0.000 | ||
miR-146bR | r | 0.588** | −0.077 | 0.495** | 0.067 | −0.127 | 0.395* | 0.421* | −0.427** | − 0.502** | |
p | 0.000 | 0.661 | 0.003 | 0.703 | 0.466 | 0.019 | 0.012 | 0.012 | 0.002 | ||
miR-15bR | r | 0.388* | −0.019 | 0.824** | 0.218 | −0.102 | 0.669** | 0.283 | −0.583** | −0.355* | |
p | 0.021 | 0.912 | 0.000 | 0.209 | 0.558 | 0.000 | 0.099 | 0.000 | 0.036 | ||
Betatrophin (ng/mL) | r | 0.461** | 0.077 | 0.601** | 0.116 | 0.043 | 0.428* | 0.458** | −0.410* | − 0.425* | |
p | 0.005 | 0.660 | 0.000 | 0.506 | 0.807 | 0.032 | 0.006 | 0.013 | 0.011 |