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Journal of Assisted Reproduction and Genetics

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01.07.2014 | Genetics

Association of MOV10L1 gene polymorphisms and male infertility in azoospermic men with complete maturation arrest

verfasst von: Homa Sarkardeh, Mehdi Totonchi, Ommolbanin Asadpour, Mohammad Ali Sadighi Gilani, Masoud Zamani Esteki, Navid Almadani, Parnaz Borjian Boroujeni, Hamid Gourabi

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 7/2014

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Abstract

Purpose

The present research was undertaken to study probable genetic variations of MOV10L1 in 30 infertile men that had complete maturation arrest in their spermatocyte levels and 70 fertile men as the control group.

Methods

We performed polymerase chain reaction single strand conformation polymorphism (PCR-SSCP) on extracted DNAs and sequencing was used to confirm the results. Identified polymorphisms in the MOV10L1 were further subjected to a haplotype analysis.

Results

We identified eight single nucleotide polymorphisms (SNPs): one missense (rs2272837) and four nonsense polymorphisms (rs2272836, rs11704548, rs2272838, rs138271) in the exonic sequences and three polymorphisms (rs12170772, rs2272840, rs17248147) in the intronic regions. With the exception of rs2272838, there was a statistically significant association in all polymorphisms between study population (P < 0.05). The result of haplotyping analysis showed ten possible haplotypes, from which five were significantly increased in infertile patients compared with the control group.

Conclusions

Our results suggest that MOV10L1 gene polymorphisms in the studied infertile males with complete maturation arrest are linked to infertility.

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Titel: Association of MOV10L1 gene polymorphisms and male infertility in azoospermic men with complete maturation arrest
verfasst von: Homa Sarkardeh
Mehdi Totonchi
Ommolbanin Asadpour
Mohammad Ali Sadighi Gilani
Masoud Zamani Esteki
Navid Almadani
Parnaz Borjian Boroujeni
Hamid Gourabi
Publikationsdatum: 01.07.2014
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics / Ausgabe 7/2014
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-014-0240-1

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Association of MOV10L1 gene polymorphisms and male infertility in azoospermic men with complete maturation arrest

Abstract

Purpose

Methods

Results

Conclusions

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Springer Medizin

Abstract

Purpose

Methods

Results

Conclusions

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