The online version of this article (doi:10.1186/1471-2261-14-6) contains supplementary material, which is available to authorized users.
The authors declare that they have no competing interests.
CZ and MM designed the study and wrote the paper. CZ and SI carried out the laboratory experiments and analyzed the data. CZ, NS and MM interpreted the results. TA, MNM and MS were responsible for sample collection, pathological data acquisition, and evaluation. All authors have contributed to, read and approved the final manuscript.
The Ryanodine receptor 3 gene (RYR3) encodes an intracellular calcium channel that mediates the efflux of Ca2+ from intracellular stores. Two single-nucleotide polymorphisms (SNPs) in the RYR3 gene have been shown to associate with stroke (rs877087) and carotid intima-media thickness (rs2229116) in two independent genome-wide association studies (GWAS) in Caucasian. We investigated the effect of these two SNPs as well as the 31.1 kilobases spanning region on atherosclerosis in Japanese population.
Atherosclerotic severity was assessed by carotid artery (n = 1374) and pathological atherosclerosis index (PAI) (n = 1262), which is a macroscopic examination of the luminal surfaces of 8 systemic arteries in consecutive autopsy samples. 4 tag SNPs in the 31.1 Kb region, rs877087, rs2132207, rs658750 and rs2229116, were genotyped and haplotypes were inferred to study the association with atherosclerotic indices.
rs877087 and rs2229116 were associated with PAI (OR = 2.07 [1.04-4.12] (95% CI), p = 0.038; and OR = 1.38 [1.02-1.86], p = 0.035, respectively). rs2229116 was also associated with common carotid atherosclerosis (OR = 1.45 [1.13-1.86], p = 0.003). The risk allele of rs2229116 was opposite from the original report. The haplotype block of this 31.1 Kb region was different between Caucasian and Japanese. Haplotype analysis revealed that only TAGG haplotype was associated with PAI (OR = 0.67 [0.48-0.94], p = 0.020) and atherosclerosis of common carotid artery (OR = 0.75 [0.58-0.98], p = 0.034).
rs877087 and rs2229116 of RYR3 gene are associated with atherosclerosis severity in Japanese. The functional difference caused by rs2229116 needs to be investigated.
Additional file 1: Table S1: Relationship between RYR3 polymorphisms (rs2132207 and rs658750) and atherosclerosis severity >75%. (DOC 46 KB)
Additional file 2: Table S2: Relationship between RYR3 polymorphisms and atherosclerosis severity >75% in age subgroups. (DOC 59 KB)
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- Association of the RYR3 gene polymorphisms with atherosclerosis in elderly Japanese population
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