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European Journal of Nutrition

Erschienen in:

01.12.2010 | Original Contribution

Astaxanthin addition improves human neutrophils function: in vitro study

verfasst von: Rita C. Macedo, Anaysa P. Bolin, Douglas P. Marin, Rosemari Otton

Erschienen in: European Journal of Nutrition | Ausgabe 8/2010

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Abstract

Purpose

The aim of the present study was to evaluate the in vitro effect of carotenoid astaxanthin (ASTA) on the phagocytic and microbicidal capacities, cytokine release, and reactive oxygen species production in human neutrophils.

Methods

The following parameters were evaluated: cytotoxic effect of ASTA on human neutrophils viability, phagocytic and microbicidal capacities of neutrophils by using Candida albicans assay, intracellular calcium mobilization (Fura 2-AM fluorescent probe), superoxide anion (lucigenin and DHE probes), hydrogen peroxide (H₂O₂, phenol red), and nitric oxide (NO·) (Griess reagent) production, activities of antioxidant enzymes (total/Mn-SOD, CAT, GPx, and GR), oxidative damages in biomolecules (TBARS assay and carbonyl groups), and cytokine (IL-6 and TNF-alpha) release.

Results

Astaxanthin significantly improves neutrophil phagocytic and microbicidal capacity, and increases the intracellular calcium concentration and NO· production. Both functional parameters were accompanied by a decrease in superoxide anion and hydrogen peroxide and IL-6 and TNF-α production. Oxidative damages in lipids and proteins were significantly decreased after ASTA-treatment.

Conclusions

Taken together our results are supportive to a beneficial effect of astaxanthin-treatment on human neutrophils function as demonstrated by increased phagocytic and fungicide capacity as well as by the reduced superoxide anion and hydrogen peroxide production, however, without affecting neutrophils capacity to kill C. albicans. This process appears to be mediated by calcium released from intracellular storages as well as nitric oxide production.

Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126CrossRef

Barrow CJ (2007) Marine nutraceuticals and functional foods. CRC Press, Boca Raton

Baynes JW, Thorpe SR (1999) Role of oxidative stress in diabetic complications: a new perspective on an old paradigm. Diabetes 48:1–9CrossRef

Bei L, Hu T, Qian ZM, Shen X (1998) Extracellular Ca2+ regulates the respiratory burst of human neutrophils. Biochim Biophys Acta 1404:475–483CrossRef

Biteau B, Labarre J, Toledano MB (2003) ATP-dependent reduction of cysteine-sulphinic acid by S. cerevisiae sulphiredoxin. Nature 425:980–984CrossRef

Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254CrossRef

Chew BP, Park JS, Wong MW, Wong TS (1999) A comparison of the anticancer activities of dietary beta-carotene, canthaxanthin and astaxanthin in mice in vivo. Anticancer Res 19:1849–1853

Choi SK, Park YS, Choi DK, Chang HI (2008) Effects of astaxanthin on the production of NO and the expression of COX-2 and iNOS in LPS-stimulated BV2 microglial cells. J Microbiol Biotechnol 18:1990–1996

Clancy RM, Leszczynska-Piziak J, Abramson SB (1992) Nitric oxide, an endothelial cell relaxation factor, inhibits neutrophil superoxide anion production via a direct action on the NADPH oxidase. J Clin Invest 90:1116–1121CrossRef

10.

Davi G, Falco A, Patrono C (2005) Lipid peroxidation in diabetes mellitus. Antioxid Redox Signal 7:256–268CrossRef

11.

Ding AH, Nathan CF, Stuehr DJ (1988) Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages. Comparison of activating cytokines and evidence for independent production. J Immunol 141:2407–2412

12.

Ewing JF, Janero DR (1995) Microplate superoxide dismutase assay employing a nonenzymatic superoxide generator. Anal Biochem 232:243–248CrossRef

13.

Fassett RG, Coombes JS (2009) Astaxanthin, oxidative stress, inflammation and cardiovascular disease. Future Cardiol 5:333–342CrossRef

14.

Fraga CG, Leibovitz BE, Tappel AL (1988) Lipid peroxidation measured as thiobarbituric acid-reactive substances in tissue slices: characterization and comparison with homogenates and microsomes. Free Radic Biol Med 4:155–161CrossRef

15.

Galligan C, Yoshimura T (2003) Phenotypic and functional changes of cytokine-activated neutrophils. In: Cassatella MA (ed) The neutrophil. Karger, Basel, pp 24–44CrossRef

16.

Ghosh S, Karin M (2002) Missing pieces in the NF-kappaB puzzle. Cell 109(Suppl):S81–S96CrossRef

17.

Grando FC, Felicio CA, Twardowschy A, Paula FM, Batista VG, Fernandes LC, Curi R, Nishiyama A (2009) Modulation of peritoneal macrophage activity by the saturation state of the fatty acid moiety of phosphatidylcholine. Braz J Med Biol Res 42:599–605CrossRef

18.

Grynkiewicz G, Poenie M, Tsien RY (1985) A new generation of Ca2+ indicators with greatly improved fluorescence properties. J Biol Chem 260:3440–3450

19.

Himpens B, Missiaen L, Casteels R (1995) Ca2+ homeostasis in vascular smooth muscle. J Vasc Res 32:207–219CrossRef

20.

Hussein G, Sankawa U, Goto H, Matsumoto K, Watanabe H (2006) Astaxanthin, a carotenoid with potential in human health and nutrition. J Nat Prod 69:443–449CrossRef

21.

Ikeuchi M, Koyama T, Takahashi J, Yazawa K (2007) Effects of astaxanthin in obese mice fed a high-fat diet. Biosci Biotechnol Biochem 71:893–899CrossRef

22.

Jyonouchi H, Hill RJ, Tomita Y, Good RA (1991) Studies of immunomodulating actions of carotenoids. I. Effects of beta-carotene and astaxanthin on murine lymphocyte functions and cell surface marker expression in in vitro culture system. Nutr Cancer 16:93–105CrossRef

23.

Jyonouchi H, Sun S, Gross M (1995) Effect of carotenoids on in vitro immunoglobulin production by human peripheral blood mononuclear cells: astaxanthin, a carotenoid without vitamin A activity, enhances in vitro immunoglobulin production in response to a T-dependent stimulant and antigen. Nutr Cancer 23:171–183CrossRef

24.

Jyonouchi H, Sun S, Tomita Y, Gross MD (1995) Astaxanthin, a carotenoid without vitamin A activity, augments antibody responses in cultures including T-helper cell clones and suboptimal doses of antigen. J Nutr 125:2483–2492

25.

Jyonouchi H, Zhang L, Gross M, Tomita Y (1994) Immunomodulating actions of carotenoids: enhancement of in vivo and in vitro antibody production to T-dependent antigens. Nutr Cancer 21:47–58CrossRef

26.

Kobayashi M (2000) In vivo antioxidant role of astaxanthin under oxidative stress in the green alga Haematococcus pluvialis. Appl Microbiol Biotechnol 54:550–555CrossRef

27.

Levy J (1999) Abnormal cell calcium homeostasis in type 2 diabetes mellitus: a new look on old disease. Endocrine 10:1–6CrossRef

28.

Liu X, Shibata T, Hisaka S, Osawa T (2009) Astaxanthin inhibits reactive oxygen species-mediated cellular toxicity in dopaminergic SH-SY5Y cells via mitochondria-targeted protective mechanism. Brain Res 1254:18–27CrossRef

29.

Lordan S, O’Neill C, O’Brien NM (2008) Effects of apigenin, lycopene and astaxanthin on 7 beta-hydroxycholesterol-induced apoptosis and Akt phosphorylation in U937 cells. Br J Nutr 100:287–296CrossRef

30.

Mannervik B (1985) Glutathione peroxidase. Methods Enzymol 113:490–495CrossRef

31.

Medina-Silva R, Barros MP, Galhardo RS, Netto LE, Colepicolo P, Menck CF (2006) Heat stress promotes mitochondrial instability and oxidative responses in yeast deficient in thiazole biosynthesis. Res Microbiol 157:275–281CrossRef

32.

Mitra S, Abraham E (2006) Participation of superoxide in neutrophil activation and cytokine production. Biochim Biophys Acta 1762:732–741

33.

Murphy ME, Kehrer JP (1989) Oxidation state of tissue thiol groups and content of protein carbonyl groups in chickens with inherited muscular dystrophy. Biochem J 260:359–364

34.

Naito Y, Uchiyama K, Aoi W, Hasegawa G, Nakamura N, Yoshida N, Maoka T, Takahashi J, Yoshikawa T (2004) Prevention of diabetic nephropathy by treatment with astaxanthin in diabetic db/db mice. Biofactors 20:49–59CrossRef

35.

Newsholme P, Haber EP, Hirabara SM, Rebelato EL, Procopio J, Morgan D, Oliveira-Emilio HC, Carpinelli AR, Curi R (2007) Diabetes associated cell stress and dysfunction: role of mitochondrial and non-mitochondrial ROS production and activity. J Physiol 583:9–24CrossRef

36.

Oliveira-Souza M, De Mello-Aires M (2000) Interaction of angiotensin II and atrial natriuretic peptide on pH(i) regulation in MDCK cells. Am J Physiol Renal Physiol 279:F944–F953

37.

Pashkow FJ, Watumull DG, Campbell CL (2008) Astaxanthin: a novel potential treatment for oxidative stress and inflammation in cardiovascular disease. Am J Cardiol 101:58D–68DCrossRef

38.

Peng HB, Rajavashisth TB, Libby P, Liao JK (1995) Nitric oxide inhibits macrophage-colony stimulating factor gene transcription in vascular endothelial cells. J Biol Chem 270:17050–17055CrossRef

39.

Pick E, Mizel D (1981) Rapid microassays for the measurement of superoxide and hydrogen peroxide production by macrophages in culture using an automatic enzyme immunoassay reader. J Immunol Methods 46:211–226CrossRef

40.

Putney JW Jr, Bird GS (1993) The signal for capacitative calcium entry. Cell 75:199–201CrossRef

41.

Rubbo H, Radi R, Trujillo M, Telleri R, Kalyanaraman B, Barnes S, Kirk M, Freeman BA (1994) Nitric oxide regulation of superoxide and peroxynitrite-dependent lipid peroxidation. Formation of novel nitrogen-containing oxidized lipid derivatives. J Biol Chem 269:26066–26075

42.

Sampaio SC, Sousa-e-Silva MC, Borelli P, Curi R, Cury Y (2001) Crotalus durissus terrificus snake venom regulates macrophage metabolism and function. J Leukoc Biol 70:551–558

43.

Spiller GA, Dewell A (2003) Safety of an astaxanthin-rich Haematococcus pluvialis algal extract: a randomized clinical trial. J Med Food 6:51–56CrossRef

44.

Stegenga ME, Van Der Crabben SN, Dessing MC, Pater JM, van den Pangaart PS, de Vos AF, Tanck MW, Roos D, Sauerwein HP, van der Poll T (2008) Effect of acute hyperglycaemia and/or hyperinsulinaemia on proinflammatory gene expression, cytokine production and neutrophil function in humans. Diabet Med 25:157–164CrossRef

45.

Stone JR, Yang S (2006) Hydrogen peroxide: a signaling messenger. Antioxid Redox Signal 8:243–270CrossRef

46.

Thomas AP, Bird GS, Hajnoczky G, Robb-Gaspers LD, Putney JW Jr (1996) Spatial and temporal aspects of cellular calcium signaling. FASEB J 10:1505–1517

47.

Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J (2007) Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 39:44–84CrossRef

48.

Yanagisawa S, Koarai A, Sugiura H, Ichikawa T, Kanda M, Tanaka R, Akamatsu K, Hirano T, Matsunaga K, Minakata Y, Ichinose M (2009) Oxidative stress augments toll-like receptor 8 mediated neutrophilic responses in healthy subjects. Respir Res 10:50CrossRef

Titel: Astaxanthin addition improves human neutrophils function: in vitro study
verfasst von: Rita C. Macedo
Anaysa P. Bolin
Douglas P. Marin
Rosemari Otton
Publikationsdatum: 01.12.2010
Verlag: Springer-Verlag
Erschienen in: European Journal of Nutrition / Ausgabe 8/2010
Print ISSN: 1436-6207
Elektronische ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-010-0103-1

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Astaxanthin addition improves human neutrophils function: in vitro study

Abstract

Purpose

Methods

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Conclusions

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Conclusions

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