nach oben

Erschienen in:

17.02.2020 | Original Article

Attenuation of the inflammatory response and polarization of macrophages by photobiomodulation

verfasst von: Kun Li, Zhuowen Liang, Jiawei Zhang, Xiaoshuang Zuo, Jiakai Sun, Qiao Zheng, Jiwei Song, Tan Ding, Xueyu Hu, Zhe Wang

Erschienen in: Lasers in Medical Science | Ausgabe 7/2020

Einloggen, um Zugang zu erhalten

Abstract

In spinal cord injury (SCI), inflammation is a major mediator of damage and loss of function and is regulated primarily by the bone marrow-derived macrophages (BMDMs). Photobiomodulation (PBM) or low-level light stimulation is known to have anti-inflammatory effects and has previously been used in the treatment of SCI, although its precise cellular mechanisms remain unclear. In the present study, the effect of PBM at 810 nm on classically activated BMDMs was evaluated to investigate the mechanisms underlying its anti-inflammatory effects. BMDMs were cultured and irradiated (810 nm, 2 mW/cm²) following stimulation with lipopolysaccharide and interferon-γ. CCK-8 assay, 2′,7′-dichlorofluorescein diacetate assay, and ELISA and western blot analysis were performed to measure cell viability, reactive oxygen species production, and inflammatory marker production, respectively. PBM irradiation of classically activated macrophages significantly increased the cell viability and inhibited reactive oxygen species generation. PBM suppressed the expression of a marker of classically activated macrophages, inducible nitric oxide synthase; decreased the mRNA expression and secretion of pro-inflammatory cytokines, tumor necrosis factor alpha, and interleukin-1 beta; and increased the secretion of monocyte chemotactic protein 1. Exposure to PBM likewise significantly reduced the expression and phosphorylation of NF-κB p65 in classically activated BMDMs. Taken together, these results suggest that PBM can successfully modulate inflammation and polarization in classically activated BMDMs. The present study provides a theoretical basis to support wider clinical application of PBM in the treatment of SCI.

Oyinbo CA (2011) Secondary injury mechanisms in traumatic spinal cord injury: a nugget of this multiply cascade. Acta Neurobiol Exp 71(2):281–299

Faden AI, Wu J, Stoica BA et al (2016) Progressive inflammation-mediated neurodegeneration after traumatic brain or spinal cord injury. Br J Pharmacol 173(4):681–691PubMed

Horn KP, Busch SA, Hawthorne AL et al (2008) Another barrier to regeneration in the CNS: activated macrophages induce extensive retraction of dystrophic axons through direct physical interactions. J Neurosci 28(38):9330–9341PubMedPubMedCentral

Busch SA, Horn KP, Silver DJ et al (2009) Overcoming macrophage-mediated axonal dieback following CNS injury. J Neurosci 29(32):9967–9976PubMedPubMedCentral

Busch SA, Hamilton J, Horn KP et al (2011) Multipotent adult progenitor cells prevent macrophage-mediated axonal dieback and promote regrowth after spinal cord injury. J Neurosci 31(3):944–953PubMedPubMedCentral

Nordendiana M, Fawtimothy D, Mckimdaniel B et al (2018) Bone marrow-derived monocytes drive the inflammatory microenvironment in local and remote regions after thoracic spinal cord injury. J Neurotrauma 2019,36(6):1–37

Greenhalgh AD, David S (2014) Differences in the phagocytic response of microglia and peripheral macrophages after spinal cord injury and its effects on cell death. J Neurosci 34(18):6316–6322PubMedPubMedCentral

Evans TA, Barkauskas DS, Myers JT et al (2014) High-resolution intravital imaging reveals that blood-derived macrophages but not resident microglia facilitate secondary axonal dieback in traumatic spinal cord injury. Exp Neurol 254(4):109–120PubMedPubMedCentral

Mosser DM, Edwards JP (2008) Exploring the full spectrum of macrophage activation. Nat Rev Immunol 8(12):958–969PubMedPubMedCentral

10.

Kigerl KA, Gensel JC, Ankeny DP et al (2009) Identification of two distinct macrophage subsets with divergent effects causing either neurotoxicity or regeneration in the injured mouse spinal cord. J Neurosci 29(43):13435–13444PubMedPubMedCentral

11.

Ren Y, Young W (2013) Managing inflammation after spinal cord injury through manipulation of macrophage function. Neural Plast 2013:945034PubMedPubMedCentral

12.

Samuel D, Antje K (2011) Repertoire of microglial and macrophage responses after spinal cord injury. Nat Rev Neurosci 12(7):388–399

13.

Guerrero AR, Uchida K, Nakajima H et al (2012) Blockade of interleukin-6 signaling inhibits the classic pathway and promotes an alternative pathway of macrophage activation after spinal cord injury in mice. J Neuroinflammation 9(1):40PubMedPubMedCentral

14.

Li F, Cheng B, Cheng J et al (2015) CCR5 blockade promotes M2 macrophage activation and improves locomotor recovery after spinal cord injury in mice. Inflammation 38(1):126–133PubMed

15.

Ji XC, Dang YY, Gao HY et al (2015) Local injection of Lenti–BDNF at the lesion site promotes M2 macrophage polarization and inhibits inflammatory response after spinal cord injury in mice. Cell Mol Neurobiol 35(6):881–890PubMed

16.

Zhang Q, Bian G, Chen P et al (2014) Aldose reductase regulates microglia/macrophages polarization through the cAMP response element-binding protein after spinal cord injury in mice. Mol Neurobiol 53(1):662–676PubMed

17.

T D, SK S, YY H et al (2012) The nuts and bolts of low-level laser (light) therapy.%A Chung H. Ann Biomed Eng 40(2):516–533

18.

Manstein D, Laubach H, Watanabe K et al (2008) Selective cryolysis: a novel method of non-invasive fat removal. Lasers Surg Med 40(9):595–604PubMed

19.

Jori G, Fabris C, Soncin M et al (2010) Photodynamic therapy in the treatment of microbial infections: basic principles and perspective applications. Lasers Surg Med 38(5):468–481

20.

Jimenez JJ, Wikramanayake TC, Bergfeld W et al (2014) Efficacy and safety of a low-level laser device in the treatment of male and female pattern hair loss: a multicenter, randomized, sham device-controlled, double-blind study. Am J Clin Dermatol 15(2):115–127PubMedPubMedCentral

21.

Lívia A, Moretti AIS, Abrah OTB et al (2012) Low-level laser therapy (808 nm) reduces inflammatory response and oxidative stress in rat tibialis anterior muscle after cryolesion. Lasers Surg Med 44(9):726–735

22.

Byrnes KR, Waynant RW, Ilev IK et al (2005) Light promotes regeneration and functional recovery and alters the immune response after spinal cord injury. Lasers Surg Med 36(3):171–185PubMed

23.

Wu X, Dmitriev AE, Cardoso MJ et al (2009) 810 nm wavelength light: an effective therapy for transected or contused rat spinal cord. Lasers Surg Med 41(1):36–41PubMed

24.

Hu D, Zhu S, Potas JR (2016) Red LED photobiomodulation reduces pain hypersensitivity and improves sensorimotor function following mild T10 hemicontusion spinal cord injury. J Neuroinflammation 13(1):200PubMedPubMedCentral

25.

26.

Song JW, Li K, Liang ZW et al (2017) Low-level laser facilitates alternatively activated macrophage/microglia polarization and promotes functional recovery after crush spinal cord injury in rats. Sci Rep 7(1):620PubMedPubMedCentral

27.

Gavish L, Perez LS, Reissman P et al (2008) Irradiation with 780 nm diode laser attenuates inflammatory cytokines while upregulating nitric oxide in LPS-stimulated macrophages: implications for the prevention of aneurysm progression. Lasers Surg Med 40(5):371–378PubMed

28.

Souza NHC, Marcondes PT, Albertini R et al (2014) Low-level laser therapy suppresses the oxidative stress-induced glucocorticoids resistance in U937 cells: relevance to cytokine secretion and histone deacetylase in alveolar macrophages. J Photochem Photobiol B Biol 130(1):327–336

29.

Ki Bum A, Seok-Seong K, Ok-Jin P et al (2014) Irradiation by gallium-aluminum-arsenate diode laser enhances the induction of nitric oxide by Porphyromonas gingivalis in RAW 264.7 cells. J Periodontol 85(9):1259–1265

30.

Fernandes KPS, Souza NHC, Mesquita-Ferrari RA et al (2015) Photobiomodulation with 660-nm and 780-nm laser on activated J774 macrophage-like cells: effect on M1 inflammatory markers. J Photochem Photobiol B Biol 153:344–351

31.

Leden REV, Cooney SJ, Ferrara TM et al (2013) 808?nm wavelength light induces a dose-dependent alteration in microglial polarization and resultant microglial induced neurite growth. Lasers Surg Med 45(4):253–263

32.

Meerpohl HG, Lohmann-Matthes ML, Fischer H (2010) Studies on the activation of mouse bone marrow-derived macrophages by the macrophage cytotoxicity factor (MCF). Eur J Immunol 6(3):213–217

33.

34.

Hamblin MR, Huang YY, Heiskanen V (2019) Non-mammalian hosts and photobiomodulation: do all life-forms respond to light? 95(1):126–Photochem Photobiol, 139

35.

Leung MCP, Lo SCL, Siu FKW et al (2010) Treatment of experimentally induced transient cerebral ischemia with low energy laser inhibits nitric oxide synthase activity and up-regulates the expression of transforming growth factor-beta 1. Lasers Surg Med 31(4):283–288

36.

Silva IHM, Andrade SCD, Fonsêca DDD et al (2016) Increase in the nitric oxide release without changes in cell viability of macrophages after laser therapy with 660 and 808 nm lasers. Lasers Med Sci 31(9):1855–1862PubMed

37.

Elena SF, Concha N, Angeles DS et al (2014) CCL2 shapes macrophage polarization by GM-CSF and M-CSF: identification of CCL2/CCR2-dependent gene expression profile. J Immunol 192(8):3858–3867

38.

Jung KM, Hae Young S, Yuexian C et al (2015) CCL2 mediates neuron-macrophage interactions to drive proregenerative macrophage activation following preconditioning injury. J Neurosci 35(48):15934–15947

39.

Alexandratou E, Yova D, Handris P et al (2002) Human fibroblast alterations induced by low power laser irradiation at the single cell level using confocal microscopy. Photochem Photobiol Sci 1(8):547–552PubMed

40.

Hume DA (2015) The many alternative faces of macrophage activation. Front Immunol 6:1–10

41.

Yu XJ, Zhang DM, Jia LL et al (2015) Inhibition of NF-κB activity in the hypothalamic paraventricular nucleus attenuates hypertension and cardiac hypertrophy by modulating cytokines and attenuating oxidative stress. Toxicol Appl Pharmacol 284(3):315–322PubMed

42.

Jurk D, Wilson CL, Passos JF et al (2014) Chronic inflammation induces telomere dysfunction and accelerates ageing in mice. Nat Commun 5(1):4172

43.

Chung IS, Kim JA, Kim JA et al (2013) Reactive oxygen species by isoflurane mediates inhibition of nuclear factor κB activation in lipopolysaccharide-induced acute inflammation of the lung. Anesth Analg 116(2):327–335PubMed

Titel: Attenuation of the inflammatory response and polarization of macrophages by photobiomodulation
verfasst von: Kun Li
Zhuowen Liang
Jiawei Zhang
Xiaoshuang Zuo
Jiakai Sun
Qiao Zheng
Jiwei Song
Tan Ding
Xueyu Hu
Zhe Wang
Publikationsdatum: 17.02.2020
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 7/2020
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-019-02941-y

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Attenuation of the inflammatory response and polarization of macrophages by photobiomodulation

Abstract

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 7/2020

Double-session micropulse transscleral laser (CYCLO G6) for the treatment of glaucoma

Monitoring enamel caries on resin-treated occlusal surfaces using quantitative light-induced fluorescence: an in vitro study

Long-term analyses of spastic muscle behavior in chronic poststroke patients after near-infrared low-level laser therapy (808 nm): a double-blinded placebo-controlled clinical trial

Exploration of day-surgery photoselective vaporization of the prostate (PVP) in Chinese population

Evolution of surface morphology of Er:YAG laser-machined human bone

Evaluation of the clinical and biochemical efficacy of erbium, chromium:ytrium-scandium-gallium-garnet (ER,CR:YSGG) laser treatment in periodontitis