Background
ANA automated reading systems
System | Pattern |
---|---|
Aklides | Homogeneous, speckled, nucleolar, centromeric, nuclear dots, cytoplasmic |
EuroPattern | Homogeneous, speckled, nucleolar, centromeric, nuclear dots, cytoplasmic |
Helios | Visual recognition by the operator |
Image Navigator | Visual recognition by the operator |
Nova View | Homogeneous, speckled, nucleolar, centromeric, nuclear dots, cytoplasmic |
Zenit G-Sight | Homogeneous, speckled, nucleolar, centromeric, nuclear dots, mitochondrial |
System | Studies, n | Patients, n | PNA, mean |
---|---|---|---|
Aklides | 3 | 1801 | 0.95 |
EuroPattern | 2 | 467 | 0.97 |
Helios | 1 | 1005 | 0.98 |
Image Navigator | 1 | 3185 | 0.99 |
Nova View | 2 | 842 | 0.95 |
Zenit G-Sight | 3 | 830 | 0.92 |
All systems | 1 | 149 | 0.96 |
Total | 13 | 8279 | 0.97 |
Comparison of the available ANA automated reading systems
Related antigens | Related diagnosis | |
---|---|---|
Nuclear patterns | ||
Homogeneous | DNA, histones, chromatin/nucleosomes | SLE, drug-induced SLE, JIA |
Peripheral/rim or nuclear envelope | Lamins, LAP1/2 gp210, nucleoporin p62; Tpr nuclear envelope and nuclear pore complex antigens | SLE, RA, PBC, myositis, autoimmune liver disease, PAPS |
Coarse speckled | U1-snRNP, U2-6 snRNP (Sm), nuclear matrix | MCTD, SLE, Raynaud, SSc, SS, UCTD |
Fine speckled | SSA/Ro, SSB/La, common to many antigens | SLE, SS, SSc, myositis, MCTD |
Dense fine speckled | DFS70/LEDGF-P75 | Healthy subjects and other inflammatory conditions |
PCNA | Auxiliary protein proliferating cell nuclear antigen: elongation factor of DNA polymerase δ | SLE, lymphoproliferative diseases, SS |
Diffuse speckled with “cloudy” mitoses | Topoisomerase-I | SSc |
Centromere | Kinetochore: CENP-A, CENP-B, CENP-C, CENP-F | SSc (limited) |
Nucleolar homogeneous | PM/Scl, RNA polymerase, To/Th , B23 phosphoprotein/numatrin | SSc, myositis, overlap myositis/SSc |
Nucleolar speckled | RNA polymerase (I to III) | SSc |
Nucleolar clumpy | U3-RNP (fibrillarin) | SSc |
Multiple/few nuclear dots | Sp100/140, PML bodies, NDP53, p80-coilin, PML bodies | PBC, CAH, SS |
Centrosome/centriole (formerly spindle apparatus) | Enolase, ninein, pericentrin | SSc, Raynaud’s phenomenon, inflammatory disease |
MSA | NuMA/centrophilin Hseg5 | RA, inflammatory conditions; pneumonia (mycoplasma) |
Cytoplasmic patterns | ||
Diffuse homogeneous (nucleoli positive) | Ribosomal proteins | SLE |
Fine speckled | Jo-1, SRP, PDH (mitochondria) | Myositides, DM, PBC, interstitial lung disease |
Discrete speckled | Endosome (early endosome antigen 1), GW/P bodies, multivesicular bodies/lysosomes | Neurological conditions, SS, SLE, RA, PBC |
Golgi complex | Golgi proteins | SLE, SS, RA, overlap syndromes, cerebellar ataxia |
Cytoplasmic fibers | Actin, cytokeratin, tropomyosin, vimentin | CAH, DM, infections and other inflammatory diseases |