Background
Materials and methods
Sample collection
Ethics statement
Viruses and antigens
Preparation of collected serum samples
Hemagglutination inhibition (HI) assay
Micro-neutralization (MN) assay
Statistical analysis
Results
Analysis of pig serum samples
Locations (number of samples)
|
Antibody titers (%)
| |||
---|---|---|---|---|
≥40
|
≥80
| |||
HI
|
MN
|
HI
|
MN
| |
Yantai (420) | 19 (4.52) | 15 (3.57) | 9 (2.14) | 6 (1.43) |
Weifang (510) | 27 (5.29) | 22 (4.31) | 12 (2.35) | 8 (1.57) |
Binzhou (326) | 14 (4.29) | 11 (3.37) | 7 (2.15) | 5 (1.53) |
Taian (320) | 16 (5.00) | 12 (3.75) | 7 (2.19) | 5 (1.56) |
Jining (200) | 9 (4.50) | 7 (3.50) | 4 (2.00) | 3 (1.50) |
Linyi (400) | 21 (5.25) | 17 (4.25) | 9 (2.25) | 5 (1.25) |
Total (2176) | 106 (4.87) | 84 (3.86) | 48 (2.21) | 32 (1.47) |
Analysis of human serum samples
Locations
|
No.
|
No. demographic description
| |||
---|---|---|---|---|---|
Median of age (range)
|
No. of female (%)
|
Occupation
|
Average occupational year
| ||
Yantai | 55 | 49 (46–60) | 18 (32.7) | Swine farm employee | 5 |
Weifang | 70 | 50 (45–58) | 20 (28.6) | Swine farm employee | 4 |
Binzhou | 42 | 46 (42–55) | 10 (23.8) | Swine farm employee | 8 |
Taian | 40 | 52 (49–61) | 12 (30.0) | Swine farm employee | 3 |
Jining | 38 | 46 (42–57) | 10 (26.3) | Swine farm employee | 5 |
Linyi | 42 | 45 (42–58) | 8 (19.0) | Swine farm employee | 5 |
Taian | 100 | 21 (19–23) | 50 (50.0) | University students | 3 |
Humans
|
Antibody titers
| |||
---|---|---|---|---|
≥40
|
≥80
| |||
HI
|
MN
|
HI
|
MN
| |
Swine farm employees (n = 287) | 6 | 4 | 4 | 2 |
Positive rate (%) | 2.09 | 1.39 | 1.39 | 0.69 |
Locations
|
Age (years)
|
Gender
|
Results
| |
---|---|---|---|---|
HI
|
MN
| |||
Weifang | 56 | Female | 160 | 80 |
Weifang | 52 | Female | 40 | -- |
Taian | 46 | Male | 40 | -- |
Linyi | 49 | Female | 160 | 160 |
Jining | 45 | Male | 80 | 40 |
Binzhou | 50 | Female | 80 | 40 |