Electronic supplementary material
Materials and methods
Administration of flagellin to conscious mice
RNA Isolation, RT-PCR and quantitative real-time PCR
Protein extraction and immunoblot analyses
Electromobility shift assay
Quantification of cytokine production
Presentation of data and statistical analysis
Results and discussion
TLR5 is expressed in mouse lung, liver, small intestine and kidney
Flagellin diffusely activates NFκB and MAP kinase signaling in tissues
Flagellin promotes widespread generation of inflammatory cytokines
Flagellin differentially triggers apoptotic signaling in mouse organs
TLR5, the specific mammalian receptor for bacterial flagellin, is expressed in the lung, kidney, liver and small intestine of mice, both at the mRNA and protein level.
Recombinant Salmonella flagellin elicits widespread innate immune responses in mouse organs, characterized by the activation of NF-κB and MAPKs.
The innate immune response to bacterial flagellin promotes systemic inflammation and triggers apoptotic signalling in the intestine.
Flagellin/TLR5 signaling may be instrumental in the pathogenesis of Gram-negative sepsis.