Background
Methods
Study design, area, and period
Populations
Study variables
Sample size and sampling technique
Data collection
Socio-demographic and clinical data
Specimen collection and transport
Laboratory methods
Culture and identification of bacterial pathogens
Antimicrobial susceptibility test
Quality control
Data analysis and interpretation
Results
Socio-demographic characteristics of participants
Possible risk factors and clinical findings
Variables | Culture results | COR (95% CI) | AOR(95% CI) |
P-value | |||
---|---|---|---|---|---|---|---|
n (%) | -ve n (%) | +ve n (%) | |||||
Previous ocular infection | Yes | 215(68.9) | 81(37.7) | 134(62.3) | 1.69(1.041–2.74) | ||
No | 97(31.1) | 49(50.5) | 48(49.5) | 1 | |||
Previous use of antimicrobials | Yes | 147(47.1) | 49 (33.3) | 98(66.7) | 1.93(1.219–3.05) | 1.84(1.132–2.97) | 0.014 |
No | 165(52.9) | 81(49.1) | 84(50.9) | 1 | 1 | ||
History of surgery | Yes | 46(14.7) | 19(41.3) | 27(58.7) | 1.02(0.539–1.92) | ||
No | 266(93.6) | 111(41.7) | 155(58.3) | 1 | |||
Duration of present illness | <1 week | 107(34.3) | 52(48.6) | 55(51.4) | 1 | 1 | |
2–4 weeks | 33(10.6) | 9(27.3) | 24(72.7) | 2.52(1.072–5.93) | 2.72(1.116–6.62) | 0.028 | |
>4 weeks | 172(55.1) | 69(40.1) | 103(59.9) | 1.41(0.868–2.29) | 1.26(0.754–2.12) | 0.34 | |
Ocular trauma | Yes | 57(18.3) | 30(52.6) | 27(47.4) | 1 | ||
No | 255(81.7) | 100(39.2) | 155(60.8) | 1.72(0.967–3.068) |
Bacteria species | Clinical features with total bacteria isolated | |||||
---|---|---|---|---|---|---|
Conjunctivitis (n = 90) n (%) | Blepharitis (n = 79) n (%) | Dacryocystitis (n = 14) n (%) | Keratitis (n = 6) n (%) | Blepharo-conjunctivitis (n = 2) n (%) | Total (n = 191) n % | |
S. aureus
| 46(51.1) | 40(50.6) | 7(50) | 3(50) | 0 | 96(50.3) |
CoNS | 28(31.1) | 26(32.9) | 7(50) | 2(33.3) | 1(50) | 64(33.5) |
S. pyognes
| 3(3.3) | 4(5.1) | 0 | 0 | 1(50) | 8(4.2) |
Klebsiella spp.
| 5(5.6) | 4(5.1) | 0 | 0 | 0 | 9(4.7) |
E.coli
| 4(4.4) | 1(1.3) | 0 | 1(16.7) | 0 | 6(3.1) |
Serratia spp. | 0 | 2(2.5) | 0 | 0 | 0 | 2(1.0) |
Citrobacter spp. | 1(1.1) | 0 | 0 | 0 | 0 | 1(0.5) |
Proteus spp. | 2(2.2) | 0 | 0 | 0 | 0 | 2(1.0) |
Entrobacter spp. | 1(1.1) | 2(2.5) | 0 | 0 | 0 | 3(1.6) |
Total n = 191 | 90(47.1) | 79(41.4) | 14(7.3) | 6(3.1) | 2(1) | 191(100) |
Prevalence of bacterial pathogens
Antimicrobial susceptibility patterns of gram positive bacterial isolates
Antimicrobials | Gram positive bacteria S or R n (%) | |||||||
---|---|---|---|---|---|---|---|---|
S.aureus,n = 96 |
CoNS,n = 64 |
S. pyogenes,n = 8 | Total, n = 168 | |||||
S (%) | R (%) | S(%) | R(%) | S(%) | R(%) | S(%) | R(%) | |
SXT | 89(92.7) | 7(7.3) | 49(76.6) | 15(23.4) | 8(100) | 0 | 146 (86.9) | 22 (13.1) |
TE | 70(72.9) | 26(27.1) | 43(67.2) | 21(32.8) | 5(62.5) | 3(37.5) | 118(70.2) | 50(29.8) |
P | 3(3.1) | 93(96.9) | 3(4.7) | 61(95.3) | 8(100) | 0 | 14(8.3) | 154(91.7) |
C | 85(88.5) | 11(11.5) | 54(84.4) | 10(15.6) | 7(77.5) | 1(12.5) | 147(87.5) | 21(12.5) |
E | 69(71.9) | 27(28.1) | 37(57.8) | 27(42.2) | 8(100) | 0 | 114(67.9) | 54 (32.1) |
DA | 89(92.7) | 7(7.3) | 61(95.3) | 3(4.7) | 5(62.5) | 3(37.5) | 155(92.3) | 13(7.7) |
CXT | 73(76) | 23(24) | 40(62.5) | 24(37.5) | - | - | 113(70.6) | 47(29.4) |
CRO | 75(78.1) | 20(20.8) | 53(82.8) | 11(17.2) | 8(100) | 0 | 136(81.0) | 32(19.0) |
CIP | 89(92.7) | 7(7.3) | 57(89.1) | 7(10.9) | - | - | 146(91.2) | 14(8.8) |
CFP | 95(99) | 1(1) | 64(100) | 0 | 8(100) | 0 | 167(99.4) | 1(0.6) |
CN | 69(71.9) | 27(28.1) | 47(73.4) | 17(26.6) | - | - | 116(72.5) | 44(27.5) |
AMP | 7(7.3) | 89(92.7) | 7(10.9) | 57(89.1) | 8(100) | 0 | 22(13.1) | 146(86.9) |
AML | 7(7.3) | 89(92.7) | 7(10.9) | 57(89.1) | 8(100) | 0 | 22(13.1) | 146(86.9) |
AMC | 79(82.3) | 17(17.7) | 53(82.8) | 11(17.2) | 8(100) | 0 | 140(83.3) | 28(16.7) |
CAZ | 13(13.5) | 83(86.5) | 11(17.2) | 53(89.1) | 8(100) | 0 | 32(19.0) | 136(81.0) |
OX | 69(71.9) | 27(28.1) | 40(62.5) | 24(37.5) | - | - | 109(68.1) | 51(31.9) |
Antimicrobial susceptibility patterns of gram negative bacterial isolates
Antimicrobials | Gram negative bacteria S, or R, n (%) | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Klebsiella spp. (n = 9) |
E. coli
(n = 6) | Entrobacter spp. (n = 3) | Seratia spp. (n = 2) | Proteus spp. (n = 2) |
Citrobacter spp. (n = 1) | Total (n = 23) | ||||||||
S (%) | R (%) | S (%) | R (%) | S (%) | R (%) | S (%) | R (%) | S (%) | R (%) | S (%) | R (%) | S (%) | R (%) | |
SXT | 6(66.7) | 3(33.3) | 6(100) | 0 | 2(66.7) | 1(33.3) | 1(50) | 1(50) | 2(100) | 0 | 1(100) | 0 | 18(78.3) | 5(21.7) |
TE | 6(66.7) | 3(33.3) | 4(66.7) | 2(33.3) | 1(33.3) | 2(66.7) | 2(100) | 0 | 1(50) | 1(50) | 1(100) | 0 | 15(65.2) | 8(34.8) |
CAZ | 5(55.6 | 4(44.4) | 5(83.3) | 1(16.7) | 3(100) | 0 | 2(100) | 0 | 2(100) | 0 | 1(100) | 0 | 18(78.3) | 5(21.7) |
C | 5(55.6) | 4(44.4) | 4(66.7) | 2(33.3) | 3(100) | 0 | 2(100) | 0 | 1(50) | 1(50) | 1(100) | 0 | 16(69.6) | 7(30.4) |
CIP | 7(77.8) | 2(22.2) | 5(83.3) | 1(16.7) | 3(100) | 0 | 2(100) | 0 | 2(100) | 0 | 1(100) | 0 | 20(87.0) | 3(13) |
CXT | 7(77.8) | 2(22.2) | 5(83.3) | 1(16.7) | 3(100) | 0 | 2(100) | 0 | 2(100) | 0 | 1(100) | 0 | 20(87.0) | 3(13) |
CRO | 6(66.7) | 3(33.3) | 5(83.3) | 1(16.7) | 2(66.7) | 1(33.3) | 1(50) | 1(50) | 2(100) | 0 | 1(100) | 0 | 17(73.9) | 6(26.1) |
CFP | 7(77.8) | 2(22.2) | 6(100) | 0 | 3(100) | 0 | 2(100) | 0 | 2(100) | 0 | 1(100) | 0 | 21(91.3) | 2(8.7) |
CN | 6(66.7) | 3(33.3) | 4(66.7) | 2(33.3) | 3(100) | 0 | 2(100) | 0 | 2(100) | 0 | 1(100) | 0 | 18(78.3) | 5(21.7) |
AMP | 0 | 9(100) | 3(50) | 3(50) | 2 (66.7) | 1(33.3) | 2(100) | 0 | 2 (100) | 0 | 1(100) | 10(43.5) | 13(56.5) | |
AML | 0 | 9(100) | 3(50) | 3(50) | 2(66.7) | 1(33.3) | 2(100) | 0 | 2(100) | 0 | 1(100) | 0 | 10(43.5) | 13(56.5) |
AMC | 8(88.9) | 1(11.1) | 5(83.3) | 1(16.7) | 2(66.7) | 1(33.3) | 2(100) | 0 | 2(100) | 0 | 1(100) | 0 | 20(87.0) | 3(13.0) |
Patterns of multidrug resistance among bacterial isolates
Types of bacteriaisolates | Total bacteria n (%) | Antimicrobial resistance pattern | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
R0 n (%) | R1 n (%) | R2 n (%) | R3 n (%) | R4 n (%) | R5 n (%) | R6 n (%) | R7 n (%) | R8 n (%) | ||
S.aureus
| 96(50.3) | 0 | 0 | 3(3.1) | 2(2.1) | 26(27.1) | 19(19.8) | 15(15.6) | 11(11.5) | 20(20.8) |
CoNS*
| 64(33.5) | 1(1.6) | 1(1.6) | 2(3.1) | 2(3.1) | 8(12.5) | 20(31.3) | 4(6.3) | 2(3.1) | 24(37.5) |
S.pyogn
| 8(4.2) | 2(25) | 5(62.5) | 1(12.5) | 0 | 0 | 0 | 0 | 0 | 0 |
Klebsella spp.
| 9(4.7) | 0 | 0 | 1(11.1) | 1(11.1) | 2(22.2) | 2(22.2) | 2(22.2) | 0 | 1(11.1) |
E.coli
| 6(3.1) | 2(33.3) | 1(16.7) | 1(16.7) | 0 | 2(33.3) | 0 | 0 | 0 | |
Entrobacter spp. | 3(1.6) | 0 | 0 | 2(66.7) | 1(33.3) | 0 | 0 | 0 | 0 | 0 |
Serratia spp. | 2(1) | 1(50) | 1(50) | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Proteus spp. | 2(1) | 1(50) | 0 | 0 | 1(50) | 0 | 0 | 0 | 0 | 0 |
Citrobacter spp. | 1(0.5) | 1(100) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Total | 191(61.2) | 8(4.2) | 7(3.7) | 10(5.2) | 8(4.2) | 36(18.8) | 43(22.5) | 21(11) | 13(6.8) | 45(23.6) |