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Erschienen in: European Journal of Nutrition 6/2017

17.06.2016 | Original Contribution

Benefits of l-alanine or l-arginine supplementation against adiposity and glucose intolerance in monosodium glutamate-induced obesity

verfasst von: Thiago R. Araujo, Israelle N. Freitas, Jean F. Vettorazzi, Thiago M. Batista, Junia C. Santos-Silva, Maria L. Bonfleur, Sandra L. Balbo, Antonio C. Boschero, Everardo M. Carneiro, Rosane A. Ribeiro

Erschienen in: European Journal of Nutrition | Ausgabe 6/2017

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Abstract

Purpose

l-alanine (Ala) and l-arginine (Arg) have been reported to regulate pancreatic β-cell physiology and to prevent body fat accumulation in diet-induced obesity. Here, we assessed growth and adiposity parameters, glucose tolerance, insulin secretion and the expression of insulin and nutrient-regulated proteins in monosodium glutamate (MSG)-obese mice supplemented with either Ala or Arg.

Methods

Male newborn C57Bl/6 mice received a daily subcutaneous injection of MSG or saline solution (CTL group), during the first 6 days of life. From 30 to 90 days of age, MSG and CTL mice received or not 2.55 % Ala (CAla or MArg groups) or 1.51 % Arg-HCl (CArg or MArg groups) in their drinking water.

Results

Adult MSG mice displayed higher adiposity associated with lower phosphorylation of the adipogenic enzyme, ACC, in adipose tissue. Glucose intolerance in MSG mice was linked to lower insulin secretion and to lower expression of IRβ in adipose tissue, as well as AS160 phosphorylation in skeletal muscle. Perigonadal fat depots were smaller in Ala and Arg mice, while retroperitoneal fat pads were decreased by Ala supplementation only. Both Ala and Arg improved fed-state glycemia as well as IRβ and pAS160 content, but only Ala led to improved glucose tolerance and insulin secretion. Adipostatic signals were increased in MAla mice, as indicated by enhanced AMPK phosphorylation and pACC content in fat depots.

Conclusions

Ala supplementation led to more pronounced metabolic improvements compared to Arg, possibly due to suppression of lipogenesis through activation of the AMPK/ACC pathway.
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Metadaten
Titel
Benefits of l-alanine or l-arginine supplementation against adiposity and glucose intolerance in monosodium glutamate-induced obesity
verfasst von
Thiago R. Araujo
Israelle N. Freitas
Jean F. Vettorazzi
Thiago M. Batista
Junia C. Santos-Silva
Maria L. Bonfleur
Sandra L. Balbo
Antonio C. Boschero
Everardo M. Carneiro
Rosane A. Ribeiro
Publikationsdatum
17.06.2016
Verlag
Springer Berlin Heidelberg
Erschienen in
European Journal of Nutrition / Ausgabe 6/2017
Print ISSN: 1436-6207
Elektronische ISSN: 1436-6215
DOI
https://doi.org/10.1007/s00394-016-1245-6

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