Background
Methods
Animals
Plant material
L-NAME –hypertension induction and treatment
Hemodynamic parameters recording
Blood and organs collection
Biochemical analysis
Statistical analysis
Results
Effects of ethylene acetate extract of Bidens pilosa on arterial blood pressure and heart rate
Parameters | Treatments | ||||
---|---|---|---|---|---|
Vehicle (10 ml/kg) | L-NAME | L-NAME + Bp (75 mg/kg) | L-NAME + Bp (150 mg/kg) | L-NAME + Losartan | |
DBP (mm Hg) | 104.14 ± 7.42 | 158.96 ± 2.29b
| 122.22 ± 8.04β
| 105.28 ± 8.22β
| 123.79 ± 1.32β
|
SBP (mm Hg) | 117.56 ± 8.66 | 180.62 ± 4.08b
| 135.64 ± 8.56β
| 119.52 ± 8.20β
| 137.72 ± 3.20β
|
MABP (mmHg) | 108.61 ± 7.75 | 166.18 ± 2.68b
| 126.69 ± 8.12β
| 110.03 ± 8.19β
| 128.43 ± 1.45β
|
HR (beat /min) | 334.21 ± 10.51 | 311.48 ± 14.14 | 314.02 ± 13.14 | 352.20 ± 15.74 | 345.30 ± 12.82 |
Effects of ethylene acetate extract of Bidens pilosa on lipid profile
Parameters | Treatments | ||||
---|---|---|---|---|---|
Vehicle (10 ml/kg) | L-NAME | L-NAME + Bp (75 mg/kg) | L-NAME +Bp (150 mg/kg) | L-NAME + Los (25 mg/kg) | |
Total Chol (mmol/L) | 1.14 ± 0.10 | 1.22 ± 0.07 | 1.13 ± 0.12 | 1.00 ± 0.14 | 1.18 ± 0.05 |
HDL chol (mmol/L) | 0.91 ± 0.13 | 0.66 ± 0.10a
| 0.83 ± 0.11α
| 0.72 ± 0.16 | 0.78 ± 0.09 |
LDL chol (mmol/L) | 0.24 ± 0.06 | 0.56 ± 0.08a
| 0.30 ± 0.04 | 0.29 ± 0.03β
| 0.40 ± 0.12 |
Triglycerides (mmol/L) | 0.61 ± 0.06 | 1.54 ± 0.05b
| 0.75 ± 0.27α
| 0.33 ± 0.11β
| 0.64 ± 0.22β
|
Atherogenic index (mmol/L) | 0.34 ± 0.01 | 0.67 ± 0.07a
| 0.39 ± 0.08α
| 0.28 ± 0.05β
| 0.51 ± 0.07 |
Effects of ethylene acetate extract of Bidens pilosa on liver and kidney functions
Parameters | Treatments | ||||
---|---|---|---|---|---|
Vehicle (10 ml/kg) | L-NAME (50 mg/kg) | L-NAME + Bp (75 mg/kg) | L-NAME + Bp (150 mg/kg) | L-NAME + Los (25 mg/kg) | |
AST (UI) | 31.03 ± 2.08 | 64.21 ± 6.73b
| 11.11 ± 0.87bβ
| 45.68 ± 5.16aβ
| 12.36 ± 1.07aβ
|
ALT (UI) | 18.51 ± 1.78 | 29.31 ± 2.30b
| 11.75 ± 1.15bβ
| 10.72 ± 0.21bβ
| 17.94 ± 2.86β
|
Creatinin (mg/mL) | 0.80 ± 0.06 | 0.88 ± 0.68 | 0.57 ± 0.06 | 0.63 ± 0.18 | 0.87 ± 0.45 |
Bilirubine (mg/L) | 4.02 ± 1.38 | 5.44 ± 1.707 | 5.63 ± 0.92 | 5.71 ± 1.058 | 8.04 ± 1.79α
|
Proteins (mg/mL) | 81.84 ± 2.57 | 70.76 ± 14.69 | 75.04 ± 14.10 | 85.20 ± 12.79 | 71.32 ± 15.94 |