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25.09.2020 | Original Research Paper

Biochanin A attenuates zymosan-induced arthritis in mice similarly to 17-β estradiol: an alternative to hormone replacement therapy?

verfasst von: Franciel Batista Felix, Jessica Maria Dantas Araújo, Elindayane Vieira de Souza, Vanessa Pinho, Enilton Aparecido Camargo, Cristiane Bani Corrêa, Renata Grespan

Erschienen in: Inflammation Research | Ausgabe 12/2020

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Abstract

Objective and design

Biochanin A (BCA), a phytoestrogen, has various pharmacological properties. This study was conducted to compare BCA’s therapeutic property against 17-β estradiol replacement therapy in zymosan-induced arthritis (ZIA) in mice. Additionally, we further investigated in vitro the anti-inflammatory action on neutrophils.

Treatment

Ovariectomized (OVX) and non-OVX mice were pretreated with BCA (1, 3 and 9 mg/kg) or estrogen (50 µg/kg) for 14 days prior to ZIA. Neutrophils were pretreated with BCA (1, 10 and 100 μM) for 1 h prior to phorbol 12-myristate 13-acetate.

Methods

Anti-inflammatory effects of BCA were evaluated by cellular infiltrate, paw edema and cytokine measurement. In vitro, apoptosis was assessed by morphology and flow cytometry. Neutrophil extracellular traps (NET) were determined by fluorescent microscopy and DNA release. Statistical differences were determined by one- or two-way ANOVA.

Results

BCA inhibited neutrophil accumulation, paw edema and proinflammatory cytokine (TNF-α and IFN-γ) and increased anti-inflammatory cytokines (IL-4 and IL-10) in OVX and non-OVX mice, similar to 17-β estradiol replacement therapy. In vitro, BCA increased apoptosis and consequently reduced NETs.

Conclusion

BCA has a notable anti-inflammatory effect, similar to 17-β estradiol, and is especially effective for treatment of ZIA. These results suggest that BCA may be promising for the treatment of postmenopausal arthritis.

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Titel: Biochanin A attenuates zymosan-induced arthritis in mice similarly to 17-β estradiol: an alternative to hormone replacement therapy?
verfasst von: Franciel Batista Felix
Jessica Maria Dantas Araújo
Elindayane Vieira de Souza
Vanessa Pinho
Enilton Aparecido Camargo
Cristiane Bani Corrêa
Renata Grespan
Publikationsdatum: 25.09.2020
Verlag: Springer International Publishing
Erschienen in: Inflammation Research / Ausgabe 12/2020
Print ISSN: 1023-3830
Elektronische ISSN: 1420-908X
DOI: https://doi.org/10.1007/s00011-020-01403-4

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Biochanin A attenuates zymosan-induced arthritis in mice similarly to 17-β estradiol: an alternative to hormone replacement therapy?

Abstract

Objective and design

Treatment

Methods

Results

Conclusion

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Abstract

Objective and design

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Methods

Results

Conclusion

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