Background
Methods
Data source and data preprocessing
Cluster analysis and differential analysis
Screening of stable feature genes and key prognostic genes
Functional enrichment analysis
Multivariate survival analysis
Validation of the key prognostic genes using an independent microarray data
Results
Data preprocessing, cluster analysis and differential analysis
Screening of stable feature genes and key prognostic genes
A Cluster | StageIII | StageIV | Total number of samples | Fisher’s exact p-value |
Cluster1 | 144 | 53 | 197 | 4.699e-06 |
Cluster2 | 260 | 31 | 291 | |
B Cluster | G2 | G3 | Total number of samples | Fisher’s exact p-value |
Cluster1 | 10 | 196 | 206 | 8.491e-06 |
Cluster2 | 59 | 279 | 338 |
Gene symbol | Gene Name |
---|---|
AXL
| AXL receptor tyrosine kinase |
EHD2
| EH domain containing 2 |
FOS
| FBJ murine osteosarcoma viral oncogene homolog |
KLF6
| Kruppel-like factor 6 |
MKS1
| Meckel syndrome, type 1 |
PDLIM2
| PDZ and LIM domain 2 |
RAB31
| RAB31, member RAS oncogene family |
TIMP3
| TIMP metallopeptidase inhibitor 3 |
WDR77
| WD repeat domain 77 |
ZFP36
| ZFP36 ring finger protein |
CSF2RB
| colony stimulating factor 2 receptor beta common subunit |
DUSP1
| dual specificity phosphatase 1 |
FMO2
| flavin containing monooxygenase 2 |
GFPT2
| glutamine-fructose-6-phosphate transaminase 2 |
GADD45B
| growth arrest and DNA damage inducible beta |
GAS1
| growth arrest specific 1 |
INHBA
| inhibin beta A |
KIF26B
| kinesin family member 26B |
MMP19
| matrix metallopeptidase 19 |
GALNT10
| polypeptide N-acetylgalactosaminyltransferase 10 |
RGS16
| regulator of G-protein signaling 16 |
RGS2
| regulator of G-protein signaling 2 |
STK3
| serine/threonine kinase 3 |
SLIT3
| slit guidance ligand 3 |
TUBB6
| tubulin beta 6 class V |
Functional enrichment analysis
Term | Description | Gene symbol | Q-value |
---|---|---|---|
GO:0042698 | ovulation cycle |
INHBA, MMP19, SLIT3, AXL
| 0.004227 |
GO:0048608 | reproductive structure development |
INHBA, MMP19, WDR77, STK3, SLIT3, AXL
| 0.004227 |
GO:2000241 | regulation of reproductive process |
INHBA, WDR77, STK3, DUSP1
| 0.004227 |
GO:0061458 | reproductive system development |
INHBA, MMP19, WDR77, STK3, SLIT3, AXL
| 0.004227 |
GO:0046660 | female sex differentiation |
INHBA, MMP19, SLIT3, AXL
| 0.004227 |
GO:0001554 | luteolysis |
MMP19, SLIT3
| 0.004227 |
GO:1901654 | response to ketone |
KLF6, SLIT3, DUSP1, FOS
| 0.009043 |
GO:0097305 | response to alcohol |
INHBA, KLF6, SLIT3, DUSP1, FOS
| 0.012039 |
GO:0022602 | ovulation cycle process |
INHBA, MMP19, SLIT3
| 0.024753 |
GO:0051591 | response to cAMP |
MMP19, DUSP1, FOS
| 0.025054 |
GO:0030728 | ovulation |
INHBA, MMP19
| 0.027454 |
GO:0008585 | female gonad development |
INHBA, MMP19, SLIT3
| 0.027454 |
GO:0046545 | development of primary female sexual characteristics |
INHBA, MMP19, SLIT3
| 0.028787 |
GO:0007548 | sex differentiation |
INHBA, MMP19, SLIT3, AXL
| 0.030429 |