Skip to main content
main-content

Tipp

Weitere Artikel dieser Ausgabe durch Wischen aufrufen

28.04.2016 | Basic Science | Ausgabe 7/2016

Graefe's Archive for Clinical and Experimental Ophthalmology 7/2016

Bit1—a potential positive regulator of epithelial–mesenchymal transition in lens epithelial cells

Zeitschrift:
Graefe's Archive for Clinical and Experimental Ophthalmology > Ausgabe 7/2016
Autoren:
Xinhua Wu, Jing Ruan, Bo Ma, Min Luo

Abstract

Purpose

Posterior capsule opacification (PCO) is a common postoperative complication of cataract surgery. Epithelial–mesenchymal transition (EMT) of lens epithelial cells (LECs) is an important initial step of PCO pathogenesis. We have previously shown that Bit1 expresses in rat LECs. In this study, we aim to investigate the role of Bit1 in the EMT of human LECs.

Methods

The expression of Bit1 was firstly detected in human PCO-attached LECs and human lens cell line SRA01/04 by real-time PCR and immunofluorescence staining. The proliferation and migration of Bit1 knockdown SRA01/04 cells were evaluated by cell counting, wound-healing assay, and transwell migration assay. The EMT of LECs was triggered by TGF-β2, and then the effect of Bit1 on EMT with a key biomarker of α-smooth muscle actin (α-SMA) was analyzed by siRNA knockdown assay, and the reversal of EMT was validated by real-time PCR and western blot.

Results

Bit1 was obviously augmented in LECs derived from PCO tissues and Bit1 expressed with high levels in the cytoplasm of cultured SRA01/04 cells. Cell proliferation, invasion, and migration, as well as α-SMA expression, were significantly decreased in Bit1 knockdown SRA01/04 cells. While TGF-β2 elevated Bit1 and α-SMA expression levels in a dose-dependent manner, with peak levels at 10 ng/ml of TGF-β2 treatment, suppression of Bit1 in SRA01/04 cells reversed the EMT process. TGF-β2 induced elevation of α-SMA expression level, as well as migration, and invasion abilities were all suppressed by Bit1 deficiency.

Conclusions

These findings reveal that Bit1 promotes TGF-β2 induced α-SMA expression and acts as an positive regulator of EMT. Suppressing Bit1 inhibits the proliferation, migration, and EMT of LECs. Bit1 may be a potential novel therapeutic target for the prevention and treatment of PCO.

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

★ PREMIUM-INHALT
e.Med Interdisziplinär

Mit e.Med Interdisziplinär erhalten Sie Zugang zu allen CME-Fortbildungen und Premium-Inhalten der Fachzeitschriften, inklusive eines Print-Abos.

Jetzt abonnieren und bis 25. Juni einen 50 € Amazon-Gutschein sichern.

Literatur
Über diesen Artikel

Weitere Artikel der Ausgabe 7/2016

Graefe's Archive for Clinical and Experimental Ophthalmology 7/2016Zur Ausgabe

Neu im Fachgebiet Augenheilkunde

01.06.2018 | themenschwerpunkt | Ausgabe 3/2018

Das Kind in der Kunst

22.05.2018 | Das Frühgeborene | Leitthema | Ausgabe 6/2018

Frühgeborenenretinopathie in welchem Stadium wie behandeln?

Aktueller Wissensstand und Ausblick

17.05.2018 | Das therapeutische und diagnostische Prinzip | Ausgabe 6/2018

Optische Kohärenztomographie bei Pathologien der Haut

18.04.2018 | Leitthema | Ausgabe 5/2018 Open Access

Trabekuläre mikroinvasive Glaukomchirurgie

Verfahren und klinische Ergebnisse