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27.02.2020 | Original Article

Blood-based gene expression profile of oxidative stress and antioxidant genes for identifying surrogate markers of liver tissue injury in chronic hepatitis C patients

verfasst von: Muhammad Shahid, Muhammad Idrees, Azeem Mehmood Butt, Syed Mohsin Raza, Iram Amin, Afza Rasul, Samia Afzal

Erschienen in: Archives of Virology | Ausgabe 4/2020

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Abstract

Oxidative stress is the process by which reactive molecules and free radicals are formed in cells. In this study, we report the blood-based gene expression profile of oxidative stress and antioxidant genes for identifying surrogate markers of liver tissue in chronic hepatitis C (CHC) patients by using real-time PCR. A total of 144 untreated patients diagnosed with CHC having genotype 3a and 20 healthy controls were selected for the present study. Liver biopsy staging and grading of CHC patients were performed using the METAVIR score. Total RNA was extracted from liver tissue and blood samples, followed by cDNA synthesis and real-time PCR. The relative expression of genes was calculated using the ΔΔCt method. The expression profile of 84 genes associated with oxidative stress and antioxidants was determined in liver tissue and blood samples. In liver tissue, 46 differentially expressed genes (upregulated, 27; downregulated, 19) were identified in CHC patients compared to normal samples. In blood, 61 genes (upregulated, 51; downregulated; 10) were significantly expressed in CHC patients. A comparison of gene expression in liver and whole blood showed that 20 genes were expressed in a similar manner in the liver and blood. The expression levels of commonly expressed liver and blood-based genes were also correlated with clinical factors in CHC patients. A receiver operating curve (ROC) analysis of oxidative stress genes (ALB, CAT, DHCR24, GPX7, PRDX5, and MBL2) showed that infections in patients with CHC can be distinguished from healthy controls. In conclusion, blood-based gene expression can reflect the behavior of oxidative stress genes in liver tissue, and this blood-based gene expression study in CHC patients explores new blood-based non-invasive biomarkers that represent liver damage.

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Adinolfi LE, Gambardella M, Andreana A, Tripodi M-f, Utili R, Ruggiero G (2001) Steatosis accelerates the progression of liver damage of chronic hepatitis C patients and correlates with specific HCV genotype and visceral obesity. Hepatology 33:1358–1364PubMed

Aleksunes LM, Manautou JE (2007) Emerging role of Nrf2 in protecting against hepatic and gastrointestinal disease. Toxicologic pathology 35:459–473PubMed

Anzola M (2004) Hepatocellular carcinoma: role of hepatitis B and hepatitis C viruses proteins in hepatocarcinogenesis. J Viral Hepat 11:383–393PubMed

Aziz H, Gil ML, Waheed Y, Adeeb U, Raza A, Bilal I, Athar MA (2011) Evaluation of prognostic factors for peg interferon alfa-2b plus ribavirin treatment on HCV infected patients in Pakistan. Infect Genet Evol 11:640–645PubMed

Bièche I, Asselah T, Laurendeau I, Vidaud D, Degot C, Paradis V, Bedossa P, Valla D-C, Marcellin P, Vidaud M (2005) Molecular profiling of early stage liver fibrosis in patients with chronic hepatitis C virus infection. Virology 332:130–144PubMed

Bisceglie AMD (1997) Hepatitis C and hepatocellular carcinoma. Hepatology 26:34S–38SPubMed

Blackham S, Baillie A, Al-Hababi F, Remlinger K, You S, Hamatake R, McGarvey MJ (2010) Gene expression profiling indicates the roles of host oxidative stress, apoptosis, lipid metabolism, and intracellular transport genes in the replication of hepatitis C virus. J Virol 84:5404–5414PubMedPubMedCentral

Brault C, Lévy P, Duponchel S, Michelet M, Sallé A, Pécheur E-I, Plissonnier M-L, Parent R, Véricel E, Ivanov AV (2016) Glutathione peroxidase 4 is reversibly induced by HCV to control lipid peroxidation and to increase virion infectivity. Gut 65:144–154PubMed

Brigelius-Flohé R, Maiorino M (2013) Glutathione peroxidases. Biochim Biophys Acta 1830:3289–3303PubMed

10.

Brown CO, Schibler J, Fitzgerald MP, Singh N, Salem K, Zhan F, Goel A (2013) Scavenger receptor class A member 3 (SCARA3) in disease progression and therapy resistance in multiple myeloma. Leuk Res 37:963–969PubMedPubMedCentral

11.

Brown KS, Keogh MJ, Tagiuri N, Grainge MJ, Presanis JS, Ryder SD, Irving WL, Ball JK, Sim RB, Hickling TP (2007) Severe fibrosis in hepatitis C virus-infected patients is associated with increased activity of the mannan-binding lectin (MBL)/MBL-associated serine protease 1 (MASP-1) complex. Clin Exp Immunol 147:90–98PubMedPubMedCentral

12.

Brown KS, Keogh MJ, Owsianka AM, Adair R, Patel AH, Arnold JN, Ball JK, Sim RB, Tarr AW, Hickling TP (2010) Specific interaction of hepatitis C virus glycoproteins with mannan binding lectin inhibits virus entry. Protein Cell 1:664–674PubMedPubMedCentral

13.

Bruhn MA, Pearson RB, Hannan RD, Sheppard KE (2010) Second AKT: the rise of SGK in cancer signalling. Growth Factors 28:394–408PubMed

14.

Burmester T, Ebner B, Weich B, Hankeln T (2002) Cytoglobin: a novel globin type ubiquitously expressed invertebrate tissues. Mol Biol Evol 19:416–421PubMed

15.

Cardin R (2001) DNA oxidative damage in leukocytes with the severity of HCV-related liver disease: validation in an open population study. J Heptol 35:587–592

16.

Casaril M, Gabrielli GB, Dusi S, Nicoli N, Bellisola G, Corrocher R (1985) Decreased activity of liver glutathione peroxidase in human hepatocellular carcinoma. Eur J Cancer 21:941–944

17.

Chen X, Cheung ST, So S, Fan ST, Barry C, Higgins J, Lai K-M, Ji J, Dudoit S, Ng IOL (2002) Gene expression patterns in human liver cancers. Mol Biol Cell 13:1929–1939PubMedPubMedCentral

18.

Cohen G, Dembiec D, Marcus J (1970) Measurement of catalase activity in tissue extracts. Anal Biochem 34:30–38PubMed

19.

Crameri A, Biondi E, Kuehnle K, Lütjohann D, Thelen KM, Perga S, Dotti CG, Nitsch RM, Ledesma MD, Mohajeri MH (2006) The role of seladin-1/DHCR24 in cholesterol biosynthesis, APP processing and Aβ generation in vivo. EMBO J 25:432–443PubMedPubMedCentral

20.

Culotta VC, Klomp LW, Strain J, Casareno RLB, Krems B, Gitlin JD (1997) The copper chaperone for superoxide dismutase. J Biol Chem 272:23469–23472PubMed

21.

d’Avigdor W, Budzinska M, Lee M, Lam R, Kench J, Stapelberg M, McLennan S, Farrell G, George J, McCaughan G (2019) Virus genotype-dependent transcriptional alterations in lipid metabolism and inflammation pathways in the hepatitis C virus-infected liver. Sci Rep 9:1–12

22.

Deng W, Wang Y, Liu Z, Cheng H, Xue Y (2014) HemI: a toolkit for illustrating heatmaps. PLoS One 9:e111988PubMedPubMedCentral

23.

DeWitte-Orr SJ, Collins SE, Bauer CMT, Bowdish DM, Mossman KL (2010) An accessory to the ‘Trinity’: SR-As are essential pathogen sensors of extracellular dsRNA, mediating entry and leading to subsequent type I IFN responses. PLoS Pathog 6:e1000829PubMedPubMedCentral

24.

Dirksen K, Spee B, Penning LC, van den Ingh TS, Burgener IA, Watson AL, Koerkamp MG, Rothuizen J, van Steenbeek FG, Fieten H (2017) Gene expression patterns in the progression of canine copper-associated chronic hepatitis. PLoS One 12:e0176826PubMedPubMedCentral

25.

Erhardt A, Hassan M, Heintges T, Häussinger D (2002) Hepatitis C virus core protein induces cell proliferation and activates ERK, JNK, and p38 MAP kinases together with the MAP kinase phosphatase MKP-1 in a HepG2 Tet-Off cell line. Virology 292:272–284PubMed

26.

Estrabaud E, Appourchaux K, Bieche I, Carrat F, Lapalus M, Lada O, Martinot-Peignoux M, Boyer N, Marcellin P, Vidaud M (2015) IFI35, mir-99a and HCV genotype to predict sustained virological response to pegylated-interferon plus ribavirin in chronic hepatitis C. PLoS One 10:e0121395PubMedPubMedCentral

27.

Eun HS, Cho SY, Lee BS, Seong IO, Kim KH (2018) Profiling cytochrome P450 family 4 gene expression in human hepatocellular carcinoma. Mol Med Rep 18:4865–4876PubMedPubMedCentral

28.

Farinati F, Cardin R, Bortolami M, Burra P, Russo FP, Rugge M, Guido M, Sergio A, Naccarato R (2007) Hepatitis C virus: from oxygen free radicals to hepatocellular carcinoma. J Viral Hepat 14:821–829PubMed

29.

Firestone G, Giampaolo J, O’Keeffe B (2003) Stimulus-dependent regulation of serum and glucocorticoid inducible protein kinase (SGK) transcription, subcellular localization and enzymatic activity. Cell Physiol Biochem 13:1–12PubMed

30.

Fried MW, Shiffman ML, Reddy KR, Smith C, Marinos G, Gonçales FL Jr, Häussinger D, Diago M, Carosi G, Dhumeaux D (2002) Peginterferon alfa-2a plus ribavirin for chronic hepatitis C virus infection. N Engl J Med 347:975–982PubMed

31.

Gong G, Waris G, Tanveer R, Siddiqui A (2001) Human hepatitis C virus NS5A protein alters intracellular calcium levels, induces oxidative stress, and activates STAT-3 and NF-κB. Proc Natl Acad Sci 98:9599–9604PubMed

32.

Guerriero E, Capone F, Accardo M, Sorice A, Costantini M, Colonna G, Castello G, Costantini S (2015) GPX4 and GPX7 over-expression in human hepatocellular carcinoma tissues. Eur J Histochem 59(4):2540. https://doi.org/10.4081/ejh.2015.2540 CrossRefPubMedPubMedCentral

33.

Guo X, Shin VY, Cho CH (2001) Modulation of heme oxygenase in tissue injury and its implication in protection against gastrointestinal diseases. Life Sci 69:3113–3119PubMed

34.

Halligan KE, Jourd’heuil FL, Jourd’heuil D (2009) Cytoglobin is expressed in the vasculature and regulates cell respiration and proliferation via nitric oxide dioxygenation. J Biol Chem 284(13):8539–8547. https://doi.org/10.1074/jbc.M808231200 CrossRefPubMedPubMedCentral

35.

Han H-J, Tokino T, Nakamura Y (1998) CSR, a scavenger receptor-like protein with a protective role against cellular damage caused by UV irradiation and oxidative stress. Hum Mol Genet 7:1039–1046PubMed

36.

Idrees M, Riazuddin S (2008) Frequency distribution of hepatitis C virus genotypes in different geographical regions of Pakistan and their possible routes of transmission. BMC Infect Dis 8:69PubMedPubMedCentral

37.

Idrees M, Rafique S, Rehman I-u, Akbar H, Yousaf MZ, Butt S, Awan Z, Manzoor S, Akram M, Aftab M (2009) Hepatitis C virus genotype 3a infection and hepatocellular carcinoma: Pakistan experience. World J Gastroenterol 15:5080PubMedPubMedCentral

38.

Immenschuh S, Ramadori G (2000) Gene regulation of heme oxygenase-1 as a therapeutic target. Biochem Pharmacol 60:1121–1128PubMed

39.

Jian WU, Karlsson K, Danielsson A (1997) Effects of vitamins E, C and catalase on bromobenzene-and hydrogen peroxide-induced intracellular oxidation and DNA single-strand breakage in Hep G2 cells. J Hepatol 26:669–677

40.

Jiang L, Hu G, Chen F, Du X, Liu B, Liu C (2017) CSR1 suppresses tumor growth and metastasis of human hepatocellular carcinoma via inhibition of HPIP. Eur Rev Med Pharmacol Sci 21:3813–3820PubMed

41.

Jin D-Y, Chae HZ, Rhee SG, Jeang K-T (1997) Regulatory role for a novel human thioredoxin peroxidase in NF-κB activation. J Biol Chem 272:30952–30961PubMed

42.

Kanzok SM, Fechner A, Bauer H, Ulschmid JK, Müller H-M, Botella-Munoz J, Schneuwly S, Schirmer RH, Becker K (2001) Substitution of the thioredoxin system for glutathione reductase in Drosophila melanogaster. Science 291:643–646PubMed

43.

Kedjouar B, De Médina P, Oulad-Abdelghani M, Payré B, Silvente-Poirot S, Favre G, Faye J-C, Poirot M (2004) Molecular characterization of the microsomal tamoxifen binding site. J Biol Chem 279:34048–34061PubMed

44.

Kobayashi T, Cohen P (1999) Activation of serum-and glucocorticoid-regulated protein kinase by agonists that activate phosphatidylinositide 3-kinase is mediated by 3-phosphoinositide-dependent protein kinase-1 (PDK1) and PDK2. Biochem J 339:319–328PubMedPubMedCentral

45.

Kundu TK, Velayutham M, Zweier JL (2012) Aldehyde oxidase functions as a superoxide generating NADH oxidase: an important redox regulated pathway of cellular oxygen radical formation. Biochemistry 51:2930–2939PubMedPubMedCentral

46.

Larrea E, Beloqui O, Muñoz-Navas M-A, Ma-P Civeira, Prieto J (1998) Superoxide dismutase in patients with chronic hepatitis C virus infection. Free Radic Biol Med 24:1235–1241PubMed

47.

Li D, Chen XQ, Li W-J, Yang Y-H, Wang J-Z, Yu ACH (2007) Cytoglobin up-regulated by hydrogen peroxide plays a protective role in oxidative stress. Neurochem Res 32:1375–1380PubMed

48.

Li L, Frei B (2009) Prolonged exposure to LPS increases iron, heme, and p22phox levels and NADPH oxidase activity in human aortic endothelial cells: inhibition by desferrioxamine. Arterioscler Thromb Vasc Biol 29:732–738PubMedPubMedCentral

49.

Liu J, Zhang G, Lv Y, Zhang X, Ying C, Yang S, Kong X, Yu Y (2017) SGK2 promotes hepatocellular carcinoma progression and mediates GSK-3β/β-catenin signaling in HCC cells. Tumor Biol 39:1010428317700408

50.

Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25:402–408PubMed

51.

Loffing J, Flores SY, Staub O (2006) Sgk kinases and their role in epithelial transport. Annu Rev Physiol 68:461–490PubMed

52.

Lozano-Sepulveda SA, Bautista-Osorio E, Merino-Mascorro JA, Varela-Rey M, Muñoz-Espinosa LE, Cordero-Perez P, Martinez-Chantar ML, Rivas-Estilla AM (2016) S-adenosyl-L-methionine modifies antioxidant-enzymes, glutathione-biosynthesis and methionine adenosyltransferases-1/2 in hepatitis C virus-expressing cells. World J Gastroenterol 22:3746PubMedPubMedCentral

53.

Lu M-Y, Huang C-I, Hsieh M-Y, Hsieh T-J, Hsi E, Tsai P-C, Tsai Y-S, Lin C-C, Hsieh M-H, Liang P-C (2016) Dynamics of PBMC gene expression in hepatitis C virus genotype 1-infected patients during combined peginterferon/ribavirin therapy. Oncotarget 7:61325PubMedPubMedCentral

54.

Lu X, Kambe F, Cao X, Kozaki Y, Kaji T, Ishii T, Seo H (2008) 3β-Hydroxysteroid-Δ24 reductase is a hydrogen peroxide scavenger, protecting cells from oxidative stress-induced apoptosis. Endocrinology 149:3267–3273PubMed

55.

Machida K, Cheng KTH, Lai C-K, Jeng K-S, Sung VMH, Lai MMC (2006) Hepatitis C virus triggers mitochondrial permeability transition with production of reactive oxygen species, leading to DNA damage and STAT3 activation. J Virol 80:7199–7207PubMedPubMedCentral

56.

Marques VB, Nascimento TB, Ribeiro RF Jr, Broseghini-Filho GB, Rossi EM, Graceli JB, dos Santos L (2015) Chronic iron overload in rats increases vascular reactivity by increasing oxidative stress and reducing nitric oxide bioavailability. Life Sci 143:89–97PubMed

57.

Minutolo A, Conti B, Grelli S, Viscomi C, Labbadia G, Balsano C (2014) Lymphocytes as liver damage mirror of HCV related adipogenesis deregulation. PLoS One 9:e92343PubMedPubMedCentral

58.

Moore KJ, Kunjathoor VV, Koehn SL, Manning JJ, Tseng AA, Silver JM, McKee M, Freeman MW (2005) Loss of receptor-mediated lipid uptake via scavenger receptor A or CD36 pathways does not ameliorate atherosclerosis in hyperlipidemic mice. J Clin Investig 115:2192–2201PubMed

59.

Moriya K, Nakagawa K, Santa T, Shintani Y, Fujie H, Miyoshi H, Tsutsumi T, Miyazawa T, Ishibashi K, Horie T (2001) Oxidative stress in the absence of inflammation in a mouse model for hepatitis C virus-associated hepatocarcinogenesis. Cancer Res 61:4365–4370PubMed

60.

Motohashi H, Yamamoto M (2004) Nrf2–Keap1 defines a physiologically important stress response mechanism. Trends Mol Med 10:549–557PubMed

61.

Nishimura T, Kohara M, Izumi K, Kasama Y, Hirata Y, Huang Y, Shuda M, Mukaidani C, Takano T, Tokunaga Y (2009) Hepatitis C virus impairs P53 via persistent over-expression of 3β-hydroxysterol δ24-reductase. J Biol Chem 284(52):36442–36452. https://doi.org/10.1074/jbc.M109.043232 CrossRefPubMedPubMedCentral

62.

Okuda M, Li K, Beard MR, Showalter LA, Scholle F, Lemon SM, Weinman SA (2002) Mitochondrial injury, oxidative stress, and antioxidant gene expression are induced by hepatitis C virus core protein. Gastroenterology 122:366–375PubMed

63.

Pallast S, Arai K, Wang X, Lo EH, Van Leyen K (2009) 12/15-Lipoxygenase targets neuronal mitochondria under oxidative stress. J Neurochem 111:882–889PubMedPubMedCentral

64.

Paracha UZ, Fatima K, Alqahtani M, Chaudhary A, Abuzenadah A, Damanhouri G, Qadri I (2013) Oxidative stress and hepatitis C virus. Virol J 10:251PubMedPubMedCentral

65.

Petit J-M, Minello A, Duvillard L, Jooste V, Monier S, Texier V, Bour J-B, Poussier A, Gambert P, Verges B (2007) Cell surface expression of LDL receptor in chronic hepatitis C: correlation with viral load. Am J Physiol Endocrinol Metab 293:E416–E420PubMed

66.

Rehermann B, Nascimbeni M (2005) Immunology of hepatitis B virus and hepatitis C virus infection. Nat Rev Immunol 5:215PubMed

67.

Reyes GR (2002) The nonstructural NS5A protein of hepatitis C virus: an expanding, multifunctional role in enhancing hepatitis C virus pathogenesis. J Biomed Sci 9:187–197PubMed

68.

Robinson LC, Marchant JS (2008) Enhanced Ca 2+ leak from ER Ca 2+ stores induced by hepatitis C NS5A protein. Biochem Biophys Res Commun 368:593–599PubMedPubMedCentral

69.

Saito M, Kohara M, Tsukiyama-Kohara K (2012) Hepatitis C virus promotes expression of the 3β-hydroxysterol δ24-reductase through Sp1. J Med Virol 84:733–746PubMed

70.

Santos CXC, Tanaka LY, Wosniak J Jr, Laurindo FRM (2009) Mechanisms and implications of reactive oxygen species generation during the unfolded protein response: roles of endoplasmic reticulum oxidoreductases, mitochondrial electron transport, and NADPH oxidase. Antioxid Redox Signal 11:2409–2427PubMed

71.

Seeff LB (2002) Natural history of chronic hepatitis C. Hepatology 36:S35–S46PubMed

72.

Shackel NA, McGuinness PH, Abbott CA, Gorrell MD, McCaughan GW (2002) Insights into the pathobiology of hepatitis C virus-associated cirrhosis: analysis of intrahepatic differential gene expression. Am J Pathol 160:641–654PubMedPubMedCentral

73.

Shao R-X, Hoshida Y, Otsuka M, Kato N, Tateishi R, Teratani T, Shiina S, Taniguchi H, Moriyama M, Kawabe T (2005) Hepatic gene expression profiles associated with fibrosis progression and hepatocarcinogenesis in hepatitis C patients. World J Gastroenterol 11:1995PubMedPubMedCentral

74.

Smith MW, Yue ZN, Korth MJ, Do HA, Boix L, Fausto N, Bruix J, Carithers RL, Katze MG (2003) Hepatitis C virus and liver disease: global transcriptional profiling and identification of potential markers. Hepatology 38:1458–1467PubMed

75.

Smith MW, Walters K-A, Korth MJ, Fitzgibbon M, Proll S, Thompson JC, Yeh MM, Shuhart MC, Furlong JC, Cox PP, Thomas DL, Phillips JD, Kushner JP, Fausto N, Carithers RL, Katze MG (2006) Gene expression patterns that correlate with hepatitis C and early progression to fibrosis in liver transplant recipients. Gastroenterology 130:179–187PubMed

76.

Tajima S, Ikeda Y, Sawada K, Yamano N, Horinouchi Y, Kihira Y, Ishizawa K, Izawa-Ishizawa Y, Kawazoe K, Tomita S (2011) Iron reduction by deferoxamine leads to amelioration of adiposity via the regulation of oxidative stress and inflammation in obese and type 2 diabetes KKAy mice. Am J Physiol Endocrinol Metab 302:E77–E86PubMed

77.

Tessier M, Woodgett JR (2006) Serum and glucocorticoid-regulated protein kinases: variations on a theme. J Cell Biochem 98:1391–1407PubMed

78.

Tong W-Y, Nagano-Fujii M, Hidajat R, Deng L, Takigawa Y, Hotta H (2002) Physical interaction between hepatitis C virus NS4B protein and CREB-RP/ATF6β. Biochem Biophys Res Commun 299:366–372PubMed

79.

Vendemiale G, Grattagliano I, Portincasa P, Serviddio G, Palasciamo G, Altomare E (2001) Oxidative stress in symptom-free HCV carriers: relation with ALT flare-up. Europ J Clin Investig 31:54–63

80.

Wang T, Weinman SA (2006) Causes and consequences of mitochondrial reactive oxygen species generation in hepatitis C. J Gastroenterol Hepatol 21:S34–S37PubMed

81.

Waris G, Tardif KD, Siddiqui A (2002) Endoplasmic reticulum (ER) stress: hepatitis C virus induces an ER-nucleus signal transduction pathway and activates NF-κB and STAT-3. Biochem Pharmacol 64:1425–1430PubMed

82.

Xu X-M, Yuan G-J, Deng J-J, Guo H-T, Xiang M, Yang F, Ge W, Chen S-Y (2012) Inhibition of 12-lipoxygenase reduces proliferation and induces apoptosis of hepatocellular carcinoma cells in vitro and in vivo. Hepatobiliary Pancreat Dis Int 11:193–202PubMed

83.

Yang M, Luna L, Sørbø JG, Alseth I, Johansen RF, Backe PH, Danbolt NC, Eide L, Bjørås M (2014) Human OXR1 maintains mitochondrial DNA integrity and counteracts hydrogen peroxide-induced oxidative stress by regulating antioxidant pathways involving p21. Free Radic Biol Med 77:41–48PubMed

84.

Yang M, Lin X, Rowe A, Rognes T, Eide L, Bjørås M (2015) Transcriptome analysis of human OXR1 depleted cells reveals its role in regulating the p53 signaling pathway. Sci Rep 5:17409PubMedPubMedCentral

85.

Yoon SY, Kim J-M, Oh J-H, Jeon Y-J, Lee D-S, Kim JH, Choi JY, Ahn BM, Kim S, Yoo H-S (2006) Gene expression profiling of human HBV-and/or HCV-associated hepatocellular carcinoma cells using expressed sequence tags. Int J Oncol 29:315–327PubMed

86.

Zein NN (2000) Clinical Significance of Hepatitis C Virus Genotypes. Clin Microbiol Rev 13:223–235PubMedPubMedCentral

Titel: Blood-based gene expression profile of oxidative stress and antioxidant genes for identifying surrogate markers of liver tissue injury in chronic hepatitis C patients
verfasst von: Muhammad Shahid
Muhammad Idrees
Azeem Mehmood Butt
Syed Mohsin Raza
Iram Amin
Afza Rasul
Samia Afzal
Publikationsdatum: 27.02.2020
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 4/2020
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-020-04564-z

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Blood-based gene expression profile of oxidative stress and antioxidant genes for identifying surrogate markers of liver tissue injury in chronic hepatitis C patients

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