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Journal of the Association for Research in Otolaryngology

Erschienen in:

12.09.2016 | Research Article

BODIPY-Conjugated Xyloside Primes Fluorescent Glycosaminoglycans in the Inner Ear of Opsanus tau

verfasst von: Holly A. Holman, Vy M. Tran, Mausam Kalita, Lynn N. Nguyen, Sailaja Arungundram, Balagurunathan Kuberan, Richard D. Rabbitt

Erschienen in: Journal of the Association for Research in Otolaryngology | Ausgabe 6/2016

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Abstract

We report on a new xyloside conjugated to BODIPY, BX and its utility to prime fluorescent glycosaminoglycans (BX-GAGs) within the inner ear in vivo. When BX is administered directly into the endolymphatic space of the oyster toadfish (Opsanus tau) inner ear, fluorescent BX-GAGs are primed and become visible in the sensory epithelia of the semicircular canals, utricle, and saccule. Confocal and 2-photon microscopy of vestibular organs fixed 4 h following BX treatment, reveal BX-GAGs constituting glycocalyces that envelop hair cell kinocilium, nerve fibers, and capillaries. In the presence of GAG-specific enzymes, the BX-GAG signals are diminished, suggesting that chondroitin sulfates are the primary GAGs primed by BX. Results are consistent with similar click-xylosides in CHO cell lines, where the xyloside enters the Golgi and preferentially initiates chondroitin sulfate B production. Introduction of BX produces a temporary block of hair cell mechanoelectrical transduction (MET) currents in the crista, reduction in background discharge rate of afferent neurons, and a reduction in sensitivity to physiological stimulation. A six-degree-of-freedom pharmacokinetic mathematical model has been applied to interpret the time course and spatial distribution of BX and BX-GAGs. Results demonstrate a new optical approach to study GAG biology in the inner ear, for tracking synthesis and localization in real time.

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Titel: BODIPY-Conjugated Xyloside Primes Fluorescent Glycosaminoglycans in the Inner Ear of Opsanus tau
verfasst von: Holly A. Holman
Vy M. Tran
Mausam Kalita
Lynn N. Nguyen
Sailaja Arungundram
Balagurunathan Kuberan
Richard D. Rabbitt
Publikationsdatum: 12.09.2016
Verlag: Springer US
Erschienen in: Journal of the Association for Research in Otolaryngology / Ausgabe 6/2016
Print ISSN: 1525-3961
Elektronische ISSN: 1438-7573
DOI: https://doi.org/10.1007/s10162-016-0585-5

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