Introduction
Colorectal cancer (CRC) is one of the most prevalent cancer types worldwide and leads to the third death of cancer-related patients. The major cause of deaths for CRC patients is distant organ metastasis especially liver metastasis [
1]. Colorectal cancer has the propensity to colonize the liver and leads to a poor prognosis in the patient. Although metastasis-related genes have been mostly clarified in CRC [
2], metastasis is a complex process and has not been fully understood.
The epithelial-mesenchymal transition (EMT) is a dynamic cellular process that is essential for the development of metastatic disease [
3]. During EMT, tumor cells with epithelial characteristics transition to those with mesenchymal characteristics through modulation of cell polarity and adhesion [
4] which contributes to cancer progression [
5]. The destruction of cadherin junction especially the downregulation of epithelial cadherin (E-cadherin) has been regarded as an important marker of EMT. The importance of cadherin in EMT has been verified in many types of human tumor [
6,
7]. In epithelial tumor, immunostaining to the E-cadherin protein demonstrates intimate relation between decrease of this protein and tumor invasiveness [
6]. Prominent regulation of E-cadherin is related to series of transcription factors such as Snail family which has a common structure of zinc finger and consists of Snail 1 (Snail), Snail 2 (Slug), Snail 3 (Smuc), and the basic helix-loop-helix (bHLH) transcription factor twist and E12/E47. Regulation factors above can bind to the E-boxes of E-cadherin promoter and thus suppress the transcription of E-cadherin in response to upstream signaling [
8]. Recently, it has been accepted that EMT is an important and complex phenomenon which determines the aggressiveness of colon cancer [
9]. Thus, to elucidate the molecular mechanisms of EMT may favor the progression of colon cancer research.
Cadherin-12 (CDH12) is a subtype of neural cadherin (N-cadherin) that belongs to cadherin family which is a flock of membrane-spanning Ca
2+-dependent homophilic adhesion receptors [
4]. CDH12 is located in chromosome 5 p14.3 and codes peptide with 88-kD molecular mass including 794 amino acid residues. CDH12 is ubiquitously expressed in a wide range of cell types. Relative studies verified that CDH12 plays a significant role in the progression of non-small-cell lung cancer and salivary adenoid cystic carcinoma [
10,
11]. However, role of CDH12 in colon cancer is still largely unknown. In this article, we presented the clinical significance of CDH12 in CRC patients’ specimens and elucidated its preminent influence on colon cancer cell proliferation as well as invasion. In addition, we found that CDH12 promotes CRC cell metastasis by promoting EMT via targeting transcriptional factor Snail. Moreover, monocyte chemotactic protein 1 (MCP1) is able to modulate CDH12 expression through induction of MCP1-induced protein (MCPIP). These findings may partially reveal possible molecular mechanisms of CDH12 promoting migration and invasion in CRC cells.
Patients, materials, and methods
Clinical specimen microarray and immunohistological analysis
Seventy-eight pairs of CRC tissue and adjacent normal tissue were included in our study. All the patients were diagnosed specifically as CRC and treated with laparoscopic surgery in Minimally Invasive Surgery Center, Ruijin Hospital, Shanghai Jiaotong University, from 2010 to 2011. The collection of specimens was authorized by Ethical Committee of Ruijin Hospital. All the patients who had received preoperative treatment such as radiation or chemotherapy, those who had lesions such as regional or organ unresectable metastasis, were excluded. The patients include 43 males and 35 females. Pathological staging of CRC tumor was performed in accordance to the TNM classification provided by World Health Organization [
12]. Fresh specimens were immediately fixed by 10 % formaldehyde after dissection and embedded with paraffin. Tissue microarrays were manufactured by Outdo Biotechnology Corporation. Immunohistological staining of tissue microarray was performed as the following: after being dewaxed at 60 °C and hydrated gradiently, the microarray was antigen retrieved by microwaving in citrate buffer (10 mM citric acid, pH 6.0), blocked in 5 % animal serum, and stained for 2 h at 37 °C by primary antibody. Secondary biotinylated anti-rabbit antibody was used to stain the microarray for 10 min at 37 °C. Microarrays were visualized by DAB, and the nucleus was counterstained by hematoxylin. Microscope was used to acquire pictures which were analyzed by two pathologists independently. Staining results were evaluated following the scoring criteria described by Shimazui T et al. [
13,
14] as we have provided previously. Expression of CDH12 was scored as positive (+), heterogeneous (+/−), or uniformly negative (−) when the CDH12 was uniformly expressed, heterogeneously expressed, or not expressed in the tissue, respectively.
Cell culture
Human CRC cell lines used in our study were purchased from American Type Culture Collection. The cell lines were preserved by Shanghai Digestive Surgery Institute. SW620 and SW1116 cell lines were cultured by Leibovitz’s L-15 medium, and HCT116 was cultured by McCoy’s 5A medium in 37 °C, 5 % CO2 incubator for use.
Protein extraction and Western blot analysis
Total protein was extracted using RIPA (Solarbio) at a 70–80 % confluence of cells. Protein lysates were reacted for 30 min on the ice and centrifuged for 15 min at 13,000 rpm. The supernatant was collected for Western blot analysis. The concentration of protein lysates was tested via bicinchoninic acid (BCA) reagent using BCA Protein Assay Kit (Thermo Scientific) by measuring optical density (OD) 562. Western blot analysis was performed as previous description. In this experiment, primary antibodies were rabbit polyclonal anti-CDH12 antibody (Abcam), 1:100; rabbit polyclonal anti-EMT marker antibodies (including E-cadherin, N-cadherin, beta-catenin (β-catenin), Snail, and Slug), 1:1000 (Cell Signaling Technology); and mouse polyclonal anti-GAPDH antibody, 1:5000. Fluorescence-labeled secondary antibody was used to visualize results by incubating for 2 h at room temperature. LI-COR Odyssey infrared fluorescence scanner was used to capture the images.
Short hairpin RNA transfection
Green fluorescent protein (GFP)-labeled short hairpin RNA (shRNA) targeting CDH12 was purchased from Gene-Pharma Corporation. Of the cells, 3 × 10
5 was seeded into each chamber 6-well plate 1 day before transfection experiment. Lipofectamine
™ 2000 (Invitrogen Corporation) was used to mediate shRNA against CDH12 into cells after 70 % confluence. According to transfection instruction, shRNA targeting non-homologous gene to CDH12 was performed as control. Forty-eight hours after transfection, cells were harvested and the protein levels of the targeted genes were assessed by Western blot. Antibiotics G418 was used to screen stable CDH12 expression cell clones. Interfering effect was evaluated by Western blot. Targeting site is listed below.
-
shCDH12-1:5′-CACCGCTGGGCAACAATTCTCCTTTTCAAGAGAAGGAGAATTGTTGCCCAGCTTTTTG-3′ (sense sequence);
-
shCDH12-2:5′-CACCGCGCAGTATAATTTCTCCATACTCGAGTATGGAGAAATTATACTGCGCTTTTTG-3′ (sense sequence);
-
shCDH12-3:5′-CACCCGGTCACATTTCCAACGTGTTCTCGAGAACACGTTGGAAATGTGACCGTTTTTG-3′ (sense sequence);
-
Negative control 5′-CACCGTTCTCCGAACGTGTCACGTCAAGAGATTACGTGACACGT TCGGAGAATTTTTTG-3′ (sense sequence).
Lentivirus stable transfection
The construction of lentivirals with plasmids containing GFP and CDH12 total gene sequences or negative control (NC) gene sequences marking with anti-antibiotic gene were purchased from Novobio Corporation. Lentivirus transfection was performed according to the manufacturer’s instruction. CDH12 protein expressions were examined by Western blot.
Cell viability assay
About 2000 cells were seeded in each well of 96-well plate and cultured in 37 °C, 5 % CO2 incubator. Three groups (shCDH12 group, shNC group, and Mock group for SW620/shCDH12 and CDH12 group, Vector group, and Blank group for HCT116/CDH12) were designed with six copies each group. The viability of cells was detected at 0, 24, 48, 72, 96, and 120 h. At the test point, 10-μL Cell Counting Kit-8 (CCK-8) was added into each well and the plate was incubated at 37 °C for 2 h followed by OD detection using spectrophotometer.
Plate clone formation assay was performed as the following: cells were resuspended to get the single-cell suspension, and then, cells were seeded in 6-well plates at a density of 1500 cells per well. After macroclones (larger than 5 mm) were formed, cell clones were fixed with methanol and stained with 1 % crystal violet.
Cell invasion and migration assays
Cell invasion and migration assays were performed by using transwell chamber (8 mm, 24-well format; Corning) which was coated with or without diluted Matrigel (BD Biosciences). Assays were performed as the following: 200-μL serum-free medium containing 3 × 105 cells was placed in the upper chamber and 600-μL culture medium with 10 % serum was added into the lower chamber as attractant. The chamber was cultured in 37 °C, 5 % CO2 incubator for 24 h. Cotton swab was used to remove reduced cells in the top chamber, and then, the chambers were fixed with methanol. Cells outside the inserts were stained with 1 % crystal violet for 30 min. Cells in five random fields of cells were photographed under microscope, and the results are calculated as means ± SD.
Immunofluorescence assay
For immunofluorescence assays, 40,000 cells were cultured in each cell of EZ slides (Millicell EZ SLIDE 8-well glass, Millipore). After being incubated for 24 h, each well was fixed in 4 % paraformaldehyde for 15 min. Cells were permeabilized with 0.1 % Triton X-100 for 15 min at room temperature, washed with phosphate-buffered saline (PBS) for three times, and blocked with PBS containing 5 % (w/v) bovine serum albumin (BSA) 1 h at room temperature. Cells were treated with anti-E-cadherin antibody (1:50, Abcam) and anti-β-catenin antibody (1:50, Abcam) and incubated overnight at 4 °C. A negative control (without primary antibody) was included on every slide. In the second day, each well were washed with PBS and incubated with iFluor™ 594 goat anti-mouse antibody (AAT Bioquest, USA) for 2 h at 37 °C. After being washed with PBS, diamidino-2-phenylindole (DAPI; Santa Cruz) was used to counterstain nucleus. Results were obtained from fluorescence microscopy (Olympus, Japan).
Nude mouse experiment
Animal experiment obeyed the ethical code and recommendation issued by Chinese Animal Community. Four-week-old male BALB/C nude mice were purchased from the Institute of Zoology, Chinese Academy of Sciences of Shanghai. In this experiment, 10 nude mice were included in each group. Of shCDH12, shNC, and Mock, 2 × 107 SW620 cells/ml were injected into nude oxter, and 2 × 107 HCT116 cells/ml of CDH12, Vector, and Blank were injected into nude oxter. After 30-day feeding, nude mice were killed by cervical decapitation and cancer nodules were excised to perform further morphological analysis. The size of tumor was calculated as the following: a*b^2/2; among this formula, “a” represents the long diameter of tumor and “b” represents the short diameter of tumor.
Statistical analysis
All the statistical analyses were performed by SAS 8.0 statistical software. The difference of CDH12 expression between tumor tissue and adjacent normal tissue was examined by Cochran-Maantel-Terpstra test. Chi-squared test and Fisher exact probability method were used to analyze the relationship between CDH12 expression level and clinical features. Survival probabilities were calculated using Kaplan-Meier method; differences between two groups were determined using the log-rank test. Multiple comparisons were performed by one-way analysis of variance. P < 0.05 was considered to be statistically significant.
Discussion
Carcinogenesis of colorectal cancer is a multistep process including complicated molecular biological events influencing cell proliferation stability and metastasis potential [
20]. Distant metastasis, especially liver metastasis, rather than the primary tumor from which these lesions originate, is responsible to the poor prognosis and high mortality of CRC patients [
21‐
23]. More than 50–60 % CRC patients suffer from metastasis; liver is the most susceptible organ in this metastasis process, and more than 80–90 % liver metastasis are unresectable lesions [
24,
25]. In our present study, we demonstrated that CDH12 acts as an oncogene in colorectal cancer cell invasion and migration. Moreover, this positive process is achieved by promoting EMT-targeting Snail.
CDH12 is a subtype of N-cadherin family expressing 88-kD protein. CDH12 can promote the migration and invasion abilities of salivary adenoid cystic carcinoma [
11]. Farzana et al. reported an unusual expression of CDH12 in CRC tissue [
26]. In our study, we performed immunohistochemistry staining in tissue microarray of 78 cases and further found that CDH12 expression was significantly increased in CRC tissues compared to adjacent normal tissue. In statistical analysis of clinical materials, we found that CDH12 high expression was associated with invasion depth of tumor tissue and lymph node lesions, independent of age, gender, tumor site, tumor histology, and tumor size. In addition, high expression of CDH12 predicted poor prognosis of CRC patients because these patients has a poorer OS and DFS compared with low CDH12 expression patients. These findings indicate the oncogenic role of CDH12 in CRC which is consistent with the findings previously.
To clearly reveal the role of CDH12 in CRC cell proliferation, we performed CCK-8 proliferation assay and clone formation assay in SW620 and HCT116 cells. We observed that downregulation of CDH12 significantly suppresses SW620 cell proliferation ability and ectopic expression of CDH12 promotes cell proliferation in HCT116. In addition, clone colony has a significant decrease/increase in SW620/shCDH12 and HCT116/CDH12 cell lines. Comparing with control group, our results showed that enforcing/repressing CDH12 on CRC cells can promote/suppress tumorigenicity in nude mice. Accordingly, our findings were supportive to promoting proliferation effect of CDH12.
To further verify the function of CDH12 in CRC cells, we performed transwell assay. Results showed suppressive role of downregulated CDH12 in CRC cell migration and invasion. Consistently, we also observed that enforcing CDH12 could promote CRC cell migration and invasion. These data indicate partially the important role of CDH12 in CRC cell metastasis which was in agreement with the findings in salivary adenoid cystic carcinoma [
6]. Considering the important role of EMT in cancer cell invasion into vessels, during EMT, a tumor cell with epithelial characteristic transitions to tumor cell with mesenchymal characteristics through modulation of cell polarity and adhesion [
3,
27], we investigated the influence of CDH12 in EMT markers. In our study, we found that CDH12 downregulation promoted the expression of epithelial marker E-cadherin and decreased the expression of mesenchymal markers Vimentin and N-cadherin; the similar results were also observed in CDH12 high-expression cell line, indicating that CDH12 could promote EMT. Moreover, we found significant change in the expression of Snail which functions in the process of downregulation of E-cadherin. The DNA-binding site of Snail is able to match the two E-boxes in E-cadherin promoters which exert the effect on downregulation of E-cadherin [
28], and such a downregulation may have a causal role in cancer migration [
29]. Accumulating evidences demonstrate the reverse correlation between Snail and E-cadherin in both human hepatocellular carcinoma (HCC) cells and tissues [
30,
31], melanoma cells [
32], and squamous cell carcinomas [
33]. In our study, we also found this reverse correlation between Snail and E-cadherin. This indicates partially, but not completely, that CDH12 might promote EMT by activating transcriptional factor Snail.
MCP1 is a 76 amino acid peptide which acts as a key CC chemokine in tumor microenvironment. It can recruit monocytes and macrophages to inflammatory sites and regulate their activities [
34]. Yoshimua et al. reported that stromal cell-derived MCP1 in primary tumors promoted metastasis of breast cancer cells to the lungs [
35]. MCPIP, which was originally found in human monocytes after treatment with MCP1, has been verified that it is able to combine with CDH12 gene and enhanced endothelial cell apoptosis, proliferation, and migration [
19]. Therefore, we detected the expressions of MCPIP and CDH12 after stimulating of MCP1 and found that MCP1 can induce MCPIP and CDH12 expressions. The elevated level of CDH12 in CRC cell is consistent with MCPIP, and the downregulation of MCPIP can also decrease the level of CDH12 protein. That means that MCP1 may act as an extracellular modulated molecule and is able to induce the expression of MCPIP by binding to relative receptor. MCPIP combines to the target CDH12 gene and promote the transcription of CDH12. Taken together, our study suggest that CDH12 might contribute to CRC cell metastasis via promoting EMT, and this process was accomplished by activating transcriptional factor Snail. Moreover, MCP1 may act as an upstream modulated molecule of CDH12.
In conclusion, we present convincing evidence showing that CDH12 is a prognostic factor in CRC patients and plays an oncogenic role in CRC cells and tumorgenicity in nude mice. Through activating transcriptional factor Snail, CDH12 regulates E-cadherin expression and promote the emergence of EMT which contributes to the metastasis of CRC cells. However, the role of CDH12 in CRC cell proliferation has not been fully understood and our findings only partially unveil the molecular mechanisms of CDH12 in EMT. Therefore, future studies are required to elucidate the function of CDH12 in CRC.
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