nach oben

Urolithiasis

Erschienen in:

06.03.2018 | Original Paper

Calcifying nanoparticles induce cytotoxicity mediated by ROS-JNK signaling pathways

verfasst von: Jihua Wu, Zhiwei Tao, Yaoliang Deng, Quan Liu, Yunlong Liu, Xiaofeng Guan, Xiang wang

Erschienen in: Urolithiasis | Ausgabe 2/2019

Einloggen, um Zugang zu erhalten

Abstract

Calcifying nanoparticles (CNPs) play an important role in kidney stone formation, but the mechanism(s) are unclear. CNPs were isolated and cultured from midstream urine of patients with kidney stones. CNP morphology and characteristics were examined by electron microscopy and electrophoresis analysis. Chemical composition was analyzed using energy-dispersive X-ray microanalysis and Western blotting. Human renal proximal convoluted tubule cell (HK-2) cultures were exposed to CNPs for 0, 12 and 72 h, and production of reactive oxygen species (ROS), mitochondrial membrane potential and apoptosis levels were evaluated. CNPs isolated from patients showed classical morphology, the size range of CNPs were 15–500 nm and negative charge; they were found to contain fetuin-A. Exposure of HK-2 cells to CNPs induced ROS production, decreased mitochondrial membrane potential and decreased cell viability. Transmission electron microscopy showed that CNPs can enter the cell by phagocytosis, and micrographs revealed signs of apoptosis and autophagy. CNPs increased the proportion of apoptotic cells, down-regulated Bcl-2 expression and up-regulated Bax expression. CNPs also up-regulated expression of LC3-B, Beclin-1and p-JNK.CNPs are phagocytosed by HK-2 cells, leading to autophagy, apoptosis and ROS production, in part through activation of JNK signaling pathways. ROS and JNK pathways may contribute to CNP-induced cell injury and kidney stone formation.

Wang WJ, Fan JY, Huang GF, Li J, Zhu X, Tian Y, Su L(2017) Prevalence of kidney stones in mainland China: a systematic review. Sci Rep 7:41630CrossRefPubMedPubMedCentral

Romero V, Akpinar H, Assimos DG (2010) Kidney stones: a global picture of prevalence, incidence, and associated risk factors. Rev Urol 12:e86–e96PubMedPubMedCentral

Sutherland JW, Parks JH, Coe FL (1985) Recurrence after a single renal stone in a community practice. Miner Electrolyte Metab 11(4):267–269PubMed

Randall A (1937) The origin and growth of renal calculi. Ann Surg 105(6):1009–1027CrossRefPubMedPubMedCentral

Çiftçioglu N, Kajander EO (1998) Interaction of nanobacteria with cultured mammalian cells. Pathophysiology 4:259–270CrossRef

Cisar JO, Xu DQ, Thompson J, Swaim W, Hu L, Dennis J (2000) An alternative interpretation of nanobacteria-induced biomineralization. PNAS 97(21):11511–11515CrossRefPubMedPubMedCentral

Miller VM, Rodgers G, Charlesworth JA, Kirkland B, Severson SR, Rasmussen TE, Yagubyan M, Rodgers JC, Cockerill FR 3rd, Folk RL, Rzewuska-Lech E, Kumar V, Farell-Baril G, Lieske JC (2004) Evidence of nanobacterial-like structures in calcified human arteries and cardiac valves. Am J Physiol Heart Circ Physiol 287(3):H1115–24. https://doi.org/10.1152/ajpheart.00075.2004 CrossRefPubMed

Martel J, Peng HH, Young D, Wu CY, Young JD (2014) Of nanobacteria, nanoparticles, biofilms and their role in health and disease: facts, fancy and future. Nanomedicine 9(4):483–499CrossRefPubMed

Ciftcioglu N, Vejdani K, Lee O, Mathew G, Aho KM, Kajander EO, McKay DS, Jones JA, Stoller ML (2008) Association between Randall’s plaque and calcifying nanoparticles. Int J Nanomed 3(1):105–115CrossRef

10.

Zhang MJ, Liu SN, Xu G, Guo YN, Fu JN, Zhang DC (2014) Cytotoxicity and apoptosis induced by nanobacteria in human breast cancer cells. Int J Nanomed 9:265–271. https://doi.org/10.2147/IJN.S54906 CrossRef

11.

Zhang M, Yang J, Shu J, Fu C, Liu S, Xu G, Zhang D (2014) Cytotoxicity induced by nanobacteria and nanohydroxyapatites in human choriocarcinoma cells. Nanoscale Res Lett 9(1):616. https://doi.org/10.1186/1556-276X-9-616 CrossRefPubMedPubMedCentral

12.

Wong T-Y, Wu C-Y, Martel J (2015) Detection and characterization of mineralo-organic nanoparticles in human kidneys. Sci Rep 5:15272CrossRefPubMedPubMedCentral

13.

Miller C, Kennington L, Cooney R, Kohjimoto Y, Cao LC, Honeyman T, Pullman J, Jonassen J, Scheid C (2000) Oxalate toxicity in renal epithelial cells: characteristics of apoptosis and necrosis. Toxicol Appl Pharmacol 162(2):132–141. https://doi.org/10.1006/taap.1999.8835 CrossRefPubMed

14.

Kajander EO, Kuronen I, Akerman KK (1997) Nanobacteria from blood: the smallest culturable autonomously replicating agent on earth. SPIE Proc 3111:420–428CrossRef

15.

Trinchieri A (1996) Epidemiology of urolithiasis. Arch Ital Urol Androl 68(4):203–249PubMed

16.

Evan AP, Lingeman JE, Coe FL, Parks JH, Bledsoe SB (2003) Randall’s plaque of patients with nephrolithiasis begins in basement membranes of thin loops of Henle. J Clin Investig 111(5):607–616. https://doi.org/10.1172/JCI200317038 CrossRefPubMedPubMedCentral

17.

Evan AP (2010) Physiopathology and etiology of stone formation in the kidney and the urinary tract. Pediatr Nephrol 25(5):831–841CrossRefPubMed

18.

Smirnov GV, Smirnov DG (2008) Nannobacteria: a new ecological threat. Contemp Probl Ecol 1:111–114

19.

Kajander EO, Ciftcioglu N (1998) Nanobacteria: an alternative mechanism for pathogenic intra- and extracellular calcification and stone formation. Proc Natl Acad Sci USA 95(14):8274–8279CrossRefPubMedPubMedCentral

20.

Kajander EO, Ciftcioglu N, Aho K, Garcia-Cuerpo E (2003) Characteristics of nanobacteria and their possible role in stone formation. Urol Res 31(2):47–54. https://doi.org/10.1007/s00240-003-0304-7 CrossRefPubMed

21.

Akerman KK, Kuikka JT, Cificioglu N (1997) Radio labeling and in vivo distribution of nanobacteria in rabbit. Proc SPIE 13:436–442CrossRef

22.

Coe FL, Evan A, Worcester E (2005) Kidney stone disease. J Clin Invest 115(10):2598–2608. https://doi.org/10.1172/JCI26662 CrossRefPubMedPubMedCentral

23.

Grases F, March JG, Conte A, Costa-Bauza A (1993) New aspects on the composition, structure and origin of calcium oxalate monohydrate calculi. Eur Urol 24(3):381–386CrossRefPubMed

24.

Abraham PA, Smith CL (1987) Evaluation of factors involved in calcium stone formation. Miner Electrolyte Metab 13(3):201–208PubMed

25.

Ciftcioglu N, Haddad RS, Golden DC, Morrison DR, McKay DS (2005) A potential cause for kidney stone formation during space flights: enhanced growth of nanobacteria in microgravity. Kidney Int 67(2):483–491. https://doi.org/10.1111/j.1523-1755.2005.67105.x CrossRefPubMed

26.

Raoult D, Drancourt M, Azza S, Nappez C, Guieu R, Rolain JM, Fourquet P, Campagna B, La Scola B, Mege JL, Mansuelle P, Lechevalier E, Berland Y, Gorvel JP, Renesto P (2008) Nanobacteria are mineralo fetuin complexes. PLoS Pathog 4(2):e41. https://doi.org/10.1371/journal.ppat.0040041 CrossRefPubMedPubMedCentral

27.

Chabriere E, Gonzalez D, Azza S, Durand P, Shiekh FA, Moal V, Baudoin JP, Pagnier I, Raoult D (2014) Fetuin is the key for nanon self-propagation. Microb Pathog 73:25–30. https://doi.org/10.1016/j.micpath.2014.05.003 CrossRefPubMed

28.

Verkoelen CF, van der Boom BG, Houtsmuller AB, Schroder FH, Romijn JC (1998) Increased calcium oxalate monohydrate crystal binding to injured renal tubular epithelial cells in culture. Am J Physiol 274(5 Pt 2):F958–965PubMed

29.

Choi AM, Ryter SW, Levine B (2013) Autophagy in human health and disease. N Engl J Med 368(7):651–662. https://doi.org/10.1056/NEJMra1205406 CrossRefPubMed

30.

Scherz-Shouval R, Elazar Z (2011) Regulation of autophagy by ROS: physiology and pathology. Trends Biochem Sci 36(1):30–38. https://doi.org/10.1016/j.tibs.2010.07.007 CrossRefPubMed

31.

Ki YW, Park JH, Lee JE, Shin IC, Koh HC (2013) JNK and p38 MAPK regulate oxidative stress and the inflammatory response in chlorpyrifos-induced apoptosis. Toxicol Lett 218(3):235–245. https://doi.org/10.1016/j.toxlet.2013.02.003 CrossRefPubMed

32.

Salazar M, Carracedo A, Salanueva IJ, Hernandez-Tiedra S, Lorente M, Egia A, Vazquez P, Blazquez C, Torres S, Garcia S, Nowak J, Fimia GM, Piacentini M, Cecconi F, Pandolfi PP, Gonzalez-Feria L, Iovanna JL, Guzman M, Boya P, Velasco G (2009) Cannabinoid action induces autophagy-mediated cell death through stimulation of ER stress in human glioma cells. J Clin Invest 119(5):1359–1372CrossRefPubMedPubMedCentral

33.

Pan X, Zhang X, Sun H, Zhang J, Yan M, Zhang H (2013) Autophagy inhibition promotes 5-fluorouraci-induced apoptosis by stimulating ROS formation in human non-small cell lung cancer A549 cells. PLoS One 8(2):e56679. https://doi.org/10.1371/journal.pone.0056679 CrossRefPubMedPubMedCentral

34.

Eisenberg-Lerner A, Bialik S, Simon HU, Kimchi A (2009) Life and death partners: apoptosis, autophagy and the cross-talk between them. Cell Death Differ 16(7):966–975. https://doi.org/10.1038/cdd.2009.33 CrossRefPubMed

35.

Nikoletopoulou V, Markaki M, Palikaras K, Tavernarakis N (2013) Crosstalk between apoptosis, necrosis and autophagy. Biochim Biophys Acta 1833(12):3448–3459. https://doi.org/10.1016/j.bbamcr.2013.06.001 CrossRefPubMed

36.

Moretti L, Cha YI, Niermann KJ, Lu B (2007) Switch between apoptosis and autophagy: radiation-induced endoplasmic reticulum stress? Cell Cycle 6(7):793–798. https://doi.org/10.4161/cc.6.7.4036 CrossRefPubMed

37.

Wong CH, Iskandar KB, Yadav SK, Hirpara JL, Loh T, Pervaiz S (2010) Simultaneous induction of non-canonical autophagy and apoptosis in cancer cells by ROS-dependent ERK and JNK activation. PLoS One 5(4):e9996. https://doi.org/10.1371/journal.pone.0009996 CrossRefPubMedPubMedCentral

38.

Wei Y, Pattingre S, Sinha S, Bassik M, Levine B (2008) JNK1-mediated phosphorylation of Bcl-2 regulates starvation-induced autophagy. Mol Cell 30(6):678–688. https://doi.org/10.1016/j.molcel.2008.06.001 CrossRefPubMedPubMedCentral

39.

Kim EM, Yang HS, Kang SW, Ho JN, Lee SB, Um HD (2008) Amplification of the gamma-irradiation-induced cell death pathway by reactive oxygen species in human U937 cells. Cell Signal 20(5):916–924. https://doi.org/10.1016/j.cellsig.2008.01.002 CrossRefPubMed

Titel: Calcifying nanoparticles induce cytotoxicity mediated by ROS-JNK signaling pathways
verfasst von: Jihua Wu
Zhiwei Tao
Yaoliang Deng
Quan Liu
Yunlong Liu
Xiaofeng Guan
Xiang wang
Publikationsdatum: 06.03.2018
Verlag: Springer Berlin Heidelberg
Erschienen in: Urolithiasis / Ausgabe 2/2019
Print ISSN: 2194-7228
Elektronische ISSN: 2194-7236
DOI: https://doi.org/10.1007/s00240-018-1048-8

Neu im Fachgebiet Urologie

22.04.2024 | Prostatakarzinom | Nachrichten

Update Urologie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Calcifying nanoparticles induce cytotoxicity mediated by ROS-JNK signaling pathways

Abstract

Neu im Fachgebiet Urologie

Stufenschema weist Prostatakarzinom zuverlässig nach

Harnwegsinfektprophylaxe: Es geht auch ohne Antibiotika

Varikozelen bei Jugendlichen: Das Für und Wider der Operation

Ureterstriktur: Innovative OP-Technik bewährt sich

Update Urologie

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 2/2019

Electron probe micro-analysis reveals the complexity of mineral deposition mechanisms in urinary stones

Journey of a cystinuric patient with a long-term follow-up from a medical stone clinic: necessity to be SaFER (stone and fragments entirely removed)

In vitro feasibility of next generation non-linear beamforming ultrasound methods to characterize and size kidney stones

Polymorphisms of the VDR gene in patients with nephrolithiasis in a Han Chinese population

Percutaneous nephrolithotomy versus retrograde intrarenal surgery for pediatric patients with upper urinary stones: a systematic review and meta-analysis

Neu im Fachgebiet Urologie

Stufenschema weist Prostatakarzinom zuverlässig nach

Harnwegsinfektprophylaxe: Es geht auch ohne Antibiotika

Varikozelen bei Jugendlichen: Das Für und Wider der Operation

Ureterstriktur: Innovative OP-Technik bewährt sich

Update Urologie