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01.11.2010 | Research | Ausgabe 1/2010 Open Access

Gut Pathogens 1/2010

Campylobacter jejuni induces transcytosis of commensal bacteria across the intestinal epithelium through M-like cells

Zeitschrift:
Gut Pathogens > Ausgabe 1/2010
Autoren:
Lisa D Kalischuk, Frances Leggett, G Douglas Inglis
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1757-4749-2-14) contains supplementary material, which is available to authorized users.

Competing interests

The authors declare that they have no competing interests.

Authors' contributions

LKT participated in the design of the study, performed experiments, conducted data analysis, and drafted the manuscript. FL conducted confocal microscopy and image analysis. GDI participated in the design of the study and edited the manuscript. All authors read and approved the final manuscript.

Abstract

Background

Recent epidemiological analyses have implicated acute Campylobacter enteritis as a factor that may incite or exacerbate inflammatory bowel disease (IBD) in susceptible individuals. We have demonstrated previously that C. jejuni disrupts the intestinal barrier function by rapidly inducing epithelial translocation of non-invasive commensal bacteria via a transcellular lipid raft-mediated mechanism ('transcytosis'). To further characterize this mechanism, the aim of this current study was to elucidate whether C. jejuni utilizes M cells to facilitate transcytosis of commensal intestinal bacteria.

Results

C. jejuni induced translocation of non-invasive E. coli across confluent Caco-2 epithelial monolayers in the absence of disrupted transepithelial electrical resistance or increased permeability to a 3 kDa dextran probe. C. jejuni-infected monolayers displayed increased numbers of cells expressing the M cell-specific marker, galectin-9, reduced numbers of enterocytes that stained with the absorptive enterocyte marker, Ulex europaeus agglutinin-1, and reduced activities of enzymes typically associated with absorptive enterocytes (namely alkaline phosphatase, lactase, and sucrase). Furthermore, in Campylobacter-infected monolayers, E. coli were observed to be internalized specifically within epithelial cells displaying M-like cell characteristics.

Conclusion

These data indicate that C. jejuni may utilize M cells to promote transcytosis of non-invasive bacteria across the intact intestinal epithelial barrier. This mechanism may contribute to the inflammatory immune responses against commensal intestinal bacteria commonly observed in IBD patients.
Zusatzmaterial
Authors’ original file for figure 1
13099_2010_40_MOESM1_ESM.tiff
Authors’ original file for figure 2
13099_2010_40_MOESM2_ESM.tiff
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