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Erschienen in: Cancer Immunology, Immunotherapy 8/2015

01.08.2015 | Original Article

CD163+CD14+ macrophages, a potential immune biomarker for malignant pleural effusion

verfasst von: Fei Wang, Li Yang, Qun Gao, Lan Huang, Liping Wang, Jing Wang, Shengdian Wang, Bin Zhang, Yi Zhang

Erschienen in: Cancer Immunology, Immunotherapy | Ausgabe 8/2015

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Abstract

Background

Malignant pleural effusion (MPE) is a common complication caused by malignant diseases. However, subjectivity, poor sensitivity, and substantial false-negative rates of cytology assay hamper accurate MPE diagnosis. The aim of this study was to assess whether CD163+CD14+ tumor-associated macrophages (TAMs) could be used as a biomarker for enabling sensitive and specific MPE diagnosis.

Methods

Pleural effusion samples and peripheral blood samples were collected from 50 MPE patients and 50 non-malignant pleural effusion (NMPE) patients, respectively. Flow cytometry was performed to analyze cell phenotypes, and RT-qPCR was used to detect cytokine expression in these monocytes and macrophages. A blinded validation study (n = 40) was subsequently performed to confirm the significance of CD163+CD14+ TAMs in MPE diagnosis. Student’s t test, rank sum test, and receiver operating characteristic curve analysis were used for statistical analysis.

Results

Notably, CD163+CD14+ cell frequency in MPE was remarkably higher than that in NMPE (P < 0.001). In a blinded validation study, a sensitivity of 78.9 % and a specificity of 100 % were obtained with CD163+CD14+ TAMs as a MPE biomarker. In total (n = 140), by using a cutoff level of 3.65 %, CD163+CD14+ cells had a sensitivity of 81.2 % and a specificity of 100 % for MPE diagnosis. Notably, MPE diagnosis by estimating CD163+CD14+ cells in pleural effusion could be obtained one week earlier than that obtained by cytological examination.

Conclusions

CD163+CD14+ macrophages could be potentially used as an immune diagnostic marker for MPE and has better assay sensitivity than that of cytological analysis.
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Metadaten
Titel
CD163+CD14+ macrophages, a potential immune biomarker for malignant pleural effusion
verfasst von
Fei Wang
Li Yang
Qun Gao
Lan Huang
Liping Wang
Jing Wang
Shengdian Wang
Bin Zhang
Yi Zhang
Publikationsdatum
01.08.2015
Verlag
Springer Berlin Heidelberg
Erschienen in
Cancer Immunology, Immunotherapy / Ausgabe 8/2015
Print ISSN: 0340-7004
Elektronische ISSN: 1432-0851
DOI
https://doi.org/10.1007/s00262-015-1701-9

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