The online version of this article (doi:10.1186/1471-230X-14-157) contains supplementary material, which is available to authorized users.
The authors declare that they have no competing interests.
JJ and XC designed the study and carried out most of the study; JJ and XC wrote the first draft of the manuscript; DC performed the molecular experiments; HM performed the pathology experiments; MJ, YW, and FK analyzed the pathological data; DC, ZJ, and FK collected patients’ clinical information; JJ performed the statistical analysis. All authors read and approved the final manuscript.
Recent studies have focused on the diagnostic and prognostic significance of CD24 and CD44 expression in human cancers. This study aimed to explore changes in CD44 and CD24 expression levels in patients with gastric cancer and to assess their prognostic values.
CD44 and CD24 expression levels were investigated immunohistochemically in tumor samples from 290 patients with non-cardia gastric adenocarcinoma, of whom 77 had paired adjacent normal gastric mucosa. CD24 and CD44 mRNA levels were determined by quantitative polymerase chain reaction in 34 patients. Serum anti-Helicobacter pylori IgG was detected by enzyme-linked immunosorbent assay. Relationships between CD44 and CD24 protein expression levels and tumor parameters were analyzed and their prognostic values were evaluated by Cox proportional hazards models.
CD24 and CD44 expression levels were significantly higher in patients with gastric cancer compared with those in paired controls (45.5% vs. 0.0%, and 61.0% vs. 0.0%, P < 0.001). Among 290 patients, the overall survival rate was significantly higher in CD44(−) compared with CD44(+) patients (log-rank test, P = 0.035). However, there was no significant correlation between CD24 expression and overall survival time (log-rank test, P = 0.115). Multivariate regression analysis indicated that positive CD44 expression (P = 0.029), TNM staging (P < 0.001), and lymphovascular invasion (P = 0.016), but not CD24 expression (P = 0.065), were independent prognostic factors in gastric cancer.
Individual expression of CD44 was associated with poor survival in patients with gastric carcinoma.
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- CD44 but not CD24 expression is related to poor prognosis in non-cardia adenocarcinoma of the stomach
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