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22.05.2018 | Original Article

Cell differentiation in cardiac myxomas: confocal microscopy and gene expression analysis after laser capture microdissection

verfasst von: Angela Pucci, Claudia Mattioli, Marco Matteucci, Daniele Lorenzini, Francesca Panvini, Simone Pacini, Chiara Ippolito, Michele Celiento, Andrea De Martino, Amelio Dolfi, Beatrice Belgio, Uberto Bortolotti, Fulvio Basolo, Giovanni Bartoloni

Erschienen in: Heart and Vessels | Ausgabe 11/2018

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Abstract

Cardiac myxomas are rare tumors with a heterogeneous cell population including properly neoplastic (lepidic), endothelial and smooth muscle cells. The assessment of neoplastic (lepidic) cell differentiation pattern is rather difficult using conventional light microscopy immunohistochemistry and/or whole tissue extracts for mRNA analyses. In a preliminary study, we investigated 20 formalin-fixed and paraffin-embedded cardiac myxomas by means of conventional immunohistochemistry; in 10/20 cases, cell differentiation was also analyzed by real-time RT-PCR after laser capture microdissection of the neoplastic cells, whereas calretinin and endothelial antigen CD31 immunoreactivity was localized in 4/10 cases by double immunofluorescence confocal microscopy. Gene expression analyses of α-smooth muscle actin, endothelial CD31 antigen, alpha-cardiac actin, matrix metalloprotease-2 (MMP2) and tissue inhibitor of matrix metalloprotease-1 (TIMP1) was performed on cDNA obtained from either microdissected neoplastic cells or whole tumor sections. We found very little or absent CD31 and α-Smooth Muscle Actin expression in the microdissected cells as compared to the whole tumors, whereas TIMP1 and MMP2 genes were highly expressed in both ones, greater levels being found in patients with embolic phenomena. α-Cardiac Actin was not detected. Confocal microscopy disclosed two different signals corresponding to calretinin-positive myxoma cells and to endothelial CD31-positive cells, respectively. In conclusion, the neoplastic (lepidic) cells showed a distinct gene expression pattern and no consistent overlapping with endothelial and smooth muscle cells or cardiac myocytes; the expression of TIMP1 and MMP2 might be related to clinical presentation; larger series studies using also systematic transcriptome analysis might be useful to confirm the present results.
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Metadaten
Titel
Cell differentiation in cardiac myxomas: confocal microscopy and gene expression analysis after laser capture microdissection
verfasst von
Angela Pucci
Claudia Mattioli
Marco Matteucci
Daniele Lorenzini
Francesca Panvini
Simone Pacini
Chiara Ippolito
Michele Celiento
Andrea De Martino
Amelio Dolfi
Beatrice Belgio
Uberto Bortolotti
Fulvio Basolo
Giovanni Bartoloni
Publikationsdatum
22.05.2018
Verlag
Springer Japan
Erschienen in
Heart and Vessels / Ausgabe 11/2018
Print ISSN: 0910-8327
Elektronische ISSN: 1615-2573
DOI
https://doi.org/10.1007/s00380-018-1189-2

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