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Erschienen in: Medical Oncology 5/2020

01.05.2020 | Original Paper

Cellular apoptosis susceptibility protein (CAS) suppresses the proliferation of breast cancer cells by upregulated cyp24a1

verfasst von: Mei Ye, Ruigang Han, Jianwu Shi, Xunda Wang, Allan Z. Zhao, Fanghong Li, Hao Chen

Erschienen in: Medical Oncology | Ausgabe 5/2020

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Abstract

Breast cancer is the most common cancer in women. Although several studies demonstrated cellular apoptosis susceptibility protein (CAS) involved in the development of breast cancer, the underlying mechanisms of CAS regulating cell processes in the breast cancer remain elusive. In the present study, we explored the possible mechanism of CAS in contributing to the cell proliferation in the breast cancer cell line MCF-7. Knockdown of CAS led to the reduction of cell viability and proliferation. Furthermore, cell cycle was arrested in G0/G1 phase after knocking down CAS with the decrease of cyclinD1. In addition, RNA-seq analysis for the CAS knockdown cells demonstrated that total eleven genes were significantly altered (Fold changes > 2). Of note, the expression of cyp24a1 was dramatically increased in the shCAS cells compared to that of shNC cells as well as confirmed by quantitative real-time polymerase chain reaction (qPCR). These observations clarified the previous conflicting results on the cell fates of the breast cells regulated by CAS and provide new insight into the role of CAS in the development of breast cancer.
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Literatur
1.
Zurück zum Zitat Bray F, et al. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2018;68(6):394–424. Bray F, et al. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2018;68(6):394–424.
2.
Zurück zum Zitat Zhan C, Zhang XY, Pang D. High expression of CSE1L is associated with poor prognosis in breast cancer. Int J Clin Exp Pathol. 2016;9(11):11788–94. Zhan C, Zhang XY, Pang D. High expression of CSE1L is associated with poor prognosis in breast cancer. Int J Clin Exp Pathol. 2016;9(11):11788–94.
3.
Zurück zum Zitat Yuksel UM, et al. Does CSE1L overexpression affect distant metastasis development in breast cancer? Oncol Res Treat. 2015;38(9):431–4.PubMed Yuksel UM, et al. Does CSE1L overexpression affect distant metastasis development in breast cancer? Oncol Res Treat. 2015;38(9):431–4.PubMed
4.
Zurück zum Zitat Lee WR, et al. CSE1L Links cAMP/PKA and Ras/ERK pathways and regulates the expressions and phosphorylations of ERK1/2, CREB, and MITF in melanoma cells. Mol Carcinog. 2016;55(11):1542–52.PubMed Lee WR, et al. CSE1L Links cAMP/PKA and Ras/ERK pathways and regulates the expressions and phosphorylations of ERK1/2, CREB, and MITF in melanoma cells. Mol Carcinog. 2016;55(11):1542–52.PubMed
5.
Zurück zum Zitat Cheng DD, et al. CSE1L interaction with MSH6 promotes osteosarcoma progression and predicts poor patient survival. Sci Rep. 2017;7(1):1–13. Cheng DD, et al. CSE1L interaction with MSH6 promotes osteosarcoma progression and predicts poor patient survival. Sci Rep. 2017;7(1):1–13.
6.
Zurück zum Zitat Qin LX, Tang ZY. The prognostic molecular markers in hepatocellular carcinoma. World J Gastroenterol. 2002;8(3):385–92.PubMedPubMedCentral Qin LX, Tang ZY. The prognostic molecular markers in hepatocellular carcinoma. World J Gastroenterol. 2002;8(3):385–92.PubMedPubMedCentral
7.
Zurück zum Zitat Shiraki K, et al. Cellular apoptosis susceptibility protein and proliferation in human hepatocellular carcinoma. Int J Mol Med. 2006;18(1):77–81.PubMed Shiraki K, et al. Cellular apoptosis susceptibility protein and proliferation in human hepatocellular carcinoma. Int J Mol Med. 2006;18(1):77–81.PubMed
8.
Zurück zum Zitat Winkler J, et al. Cellular apoptosis susceptibility (CAS) is linked to integrin β1 and required for tumor cell migration and invasion in hepatocellular carcinoma (HCC). Oncotarget. 2016;7(16):22883–92.PubMedPubMedCentral Winkler J, et al. Cellular apoptosis susceptibility (CAS) is linked to integrin β1 and required for tumor cell migration and invasion in hepatocellular carcinoma (HCC). Oncotarget. 2016;7(16):22883–92.PubMedPubMedCentral
9.
Zurück zum Zitat Liu GM, et al. Identification of a six-gene signature predicting overall survival for hepatocellular carcinoma. Cancer Cell Int. 2019;19:138.PubMedPubMedCentral Liu GM, et al. Identification of a six-gene signature predicting overall survival for hepatocellular carcinoma. Cancer Cell Int. 2019;19:138.PubMedPubMedCentral
10.
Zurück zum Zitat Pimiento JM, et al. Knockdown of cse1l gene in colorectal cancer reduces tumorigenesis in vitro. Am J Pathol. 2016;186(10):2761–8.PubMedPubMedCentral Pimiento JM, et al. Knockdown of cse1l gene in colorectal cancer reduces tumorigenesis in vitro. Am J Pathol. 2016;186(10):2761–8.PubMedPubMedCentral
11.
Zurück zum Zitat Jiang MC, et al. CSE1L modulates Ras-induced cancer cell invasion: correlation of K-Ras mutation and CSE1L expression in colorectal cancer progression. Am J Surg. 2013;206(3):418–27.PubMed Jiang MC, et al. CSE1L modulates Ras-induced cancer cell invasion: correlation of K-Ras mutation and CSE1L expression in colorectal cancer progression. Am J Surg. 2013;206(3):418–27.PubMed
12.
Zurück zum Zitat Tai CJ, Su TC, Jiang MC. Correlations between cytoplasmic CSE1L in neoplastic colorectal glands and depth of tumor penetration and cancer stage. J Transl Med. 2013;11:29.PubMedPubMedCentral Tai CJ, Su TC, Jiang MC. Correlations between cytoplasmic CSE1L in neoplastic colorectal glands and depth of tumor penetration and cancer stage. J Transl Med. 2013;11:29.PubMedPubMedCentral
13.
Zurück zum Zitat Tsao TY, et al. Function of CSE1L/CAS in the secretion of HT-29 human colorectal cells and its expression in human colon. Mol Cell Biochem. 2009;327(1–2):163–70.PubMed Tsao TY, et al. Function of CSE1L/CAS in the secretion of HT-29 human colorectal cells and its expression in human colon. Mol Cell Biochem. 2009;327(1–2):163–70.PubMed
14.
Zurück zum Zitat Sillars-Hardebol AH, et al. TPX2 and AURKA promote 20q amplicon-driven colorectal adenoma to carcinoma progression. Gut. 2012;61(11):1568–75.PubMed Sillars-Hardebol AH, et al. TPX2 and AURKA promote 20q amplicon-driven colorectal adenoma to carcinoma progression. Gut. 2012;61(11):1568–75.PubMed
15.
Zurück zum Zitat Li KK, et al. MIR-137 suppresses growth and invasion, is downregulated in oligodendroglial tumors and targets CSE1L. Brain Pathol. 2013;23(4):426–39.PubMed Li KK, et al. MIR-137 suppresses growth and invasion, is downregulated in oligodendroglial tumors and targets CSE1L. Brain Pathol. 2013;23(4):426–39.PubMed
16.
17.
Zurück zum Zitat Liu J, et al. Expression of cellular apoptosis susceptibility (CAS) in the human testis and testicular germ cell tumors. Med Oncol. 2019;36(7):61.PubMed Liu J, et al. Expression of cellular apoptosis susceptibility (CAS) in the human testis and testicular germ cell tumors. Med Oncol. 2019;36(7):61.PubMed
18.
Zurück zum Zitat Lorenzato A, et al. The cellular apoptosis susceptibility CAS/CSE1L gene protects ovarian cancer cells from death by suppressing RASSF1C. FASEB J. 2012;26(6):2446–56.PubMed Lorenzato A, et al. The cellular apoptosis susceptibility CAS/CSE1L gene protects ovarian cancer cells from death by suppressing RASSF1C. FASEB J. 2012;26(6):2446–56.PubMed
19.
Zurück zum Zitat Dong Q, et al. Roles of the CSE1L-mediated nuclear import pathway in epigenetic silencing. Proc Natl Acad Sci USA. 2018;115(17):E4013–E40224022.PubMed Dong Q, et al. Roles of the CSE1L-mediated nuclear import pathway in epigenetic silencing. Proc Natl Acad Sci USA. 2018;115(17):E4013–E40224022.PubMed
20.
Zurück zum Zitat Kutay U, et al. Export of importin alpha from the nucleus is mediated by a specific nuclear transport factor. Cell. 1997;90(6):1061–71.PubMed Kutay U, et al. Export of importin alpha from the nucleus is mediated by a specific nuclear transport factor. Cell. 1997;90(6):1061–71.PubMed
21.
Zurück zum Zitat Ewings KE, Ryan KM. Hzf and hCAS/CSE1L: making the right choice in p53-mediated tumour suppression. Cell Res. 2007;17(10):829–31.PubMed Ewings KE, Ryan KM. Hzf and hCAS/CSE1L: making the right choice in p53-mediated tumour suppression. Cell Res. 2007;17(10):829–31.PubMed
22.
Zurück zum Zitat Tanaka T, et al. hCAS/CSE1L associates with chromatin and regulates expression of select p53 target genes. Cell. 2007;130(4):638–50.PubMed Tanaka T, et al. hCAS/CSE1L associates with chromatin and regulates expression of select p53 target genes. Cell. 2007;130(4):638–50.PubMed
23.
Zurück zum Zitat Zhu JH, et al. Suppression of cellular apoptosis susceptibility (CSE1L) inhibits proliferation and induces apoptosis in colorectal cancer cells. Asian Pac J Cancer Prev. 2013;14(2):1017–21.PubMed Zhu JH, et al. Suppression of cellular apoptosis susceptibility (CSE1L) inhibits proliferation and induces apoptosis in colorectal cancer cells. Asian Pac J Cancer Prev. 2013;14(2):1017–21.PubMed
24.
Zurück zum Zitat Liao CF, et al. CSE1L, a novel microvesicle membrane protein, mediates Ras-triggered microvesicle generation and metastasis of tumor cells. Mol Med. 2012;18:1269–80.PubMedPubMedCentral Liao CF, et al. CSE1L, a novel microvesicle membrane protein, mediates Ras-triggered microvesicle generation and metastasis of tumor cells. Mol Med. 2012;18:1269–80.PubMedPubMedCentral
25.
Zurück zum Zitat Xu R, et al. Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. Methods. 2015;87:11–25.PubMed Xu R, et al. Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. Methods. 2015;87:11–25.PubMed
26.
Zurück zum Zitat Tai CJ, et al. Cellular apoptosis susceptibility (CSE1L/CAS) protein in cancer metastasis and chemotherapeutic drug-induced apoptosis. J Exp Clin Cancer Res. 2010;29(1):110.PubMedPubMedCentral Tai CJ, et al. Cellular apoptosis susceptibility (CSE1L/CAS) protein in cancer metastasis and chemotherapeutic drug-induced apoptosis. J Exp Clin Cancer Res. 2010;29(1):110.PubMedPubMedCentral
27.
Zurück zum Zitat Bera TK, et al. Cse1l is essential for early embryonic growth and development. Mol Cell Biol. 2001;21(20):7020–4.PubMedPubMedCentral Bera TK, et al. Cse1l is essential for early embryonic growth and development. Mol Cell Biol. 2001;21(20):7020–4.PubMedPubMedCentral
28.
Zurück zum Zitat Yuksel UM, et al. The relationship between CSE1L expression and axillary lymph node metastasis in breast cancer. Tumori. 2015;101(2):194–8.PubMed Yuksel UM, et al. The relationship between CSE1L expression and axillary lymph node metastasis in breast cancer. Tumori. 2015;101(2):194–8.PubMed
29.
Zurück zum Zitat Liu C, et al. CSE1L participates in regulating cell mitosis in human seminoma. Cell Prolif. 2019;52(2):e12549.PubMed Liu C, et al. CSE1L participates in regulating cell mitosis in human seminoma. Cell Prolif. 2019;52(2):e12549.PubMed
30.
Zurück zum Zitat Li Y, et al. CSE1L silence inhibits the growth and metastasis in gastric cancer by repressing GPNMB via positively regulating transcription factor MITF. J Cell Physiol. 2020;235(3):2071–9.PubMed Li Y, et al. CSE1L silence inhibits the growth and metastasis in gastric cancer by repressing GPNMB via positively regulating transcription factor MITF. J Cell Physiol. 2020;235(3):2071–9.PubMed
31.
Zurück zum Zitat Liao CF, et al. CSE1L/CAS, the cellular apoptosis susceptibility protein, enhances invasion and metastasis but not proliferation of cancer cells. J Exp Clin Cancer Res. 2008;27:15.PubMedPubMedCentral Liao CF, et al. CSE1L/CAS, the cellular apoptosis susceptibility protein, enhances invasion and metastasis but not proliferation of cancer cells. J Exp Clin Cancer Res. 2008;27:15.PubMedPubMedCentral
32.
Zurück zum Zitat Tang Z, et al. GEPIA: a web server for cancer and normal gene expression profiling and interactive analyses. Nucleic Acids Res. 2017;45(W1):W98–W102.PubMedPubMedCentral Tang Z, et al. GEPIA: a web server for cancer and normal gene expression profiling and interactive analyses. Nucleic Acids Res. 2017;45(W1):W98–W102.PubMedPubMedCentral
33.
Zurück zum Zitat Gyorffy B, Schafer R. Meta-analysis of gene expression profiles related to relapse-free survival in 1,079 breast cancer patients. Breast Cancer Res Treat. 2009;118(3):433–41.PubMed Gyorffy B, Schafer R. Meta-analysis of gene expression profiles related to relapse-free survival in 1,079 breast cancer patients. Breast Cancer Res Treat. 2009;118(3):433–41.PubMed
34.
Zurück zum Zitat Gyorffy B, et al. An online survival analysis tool to rapidly assess the effect of 22,277 genes on breast cancer prognosis using microarray data of 1,809 patients. Breast Cancer Res Treat. 2010;123(3):725–31.PubMed Gyorffy B, et al. An online survival analysis tool to rapidly assess the effect of 22,277 genes on breast cancer prognosis using microarray data of 1,809 patients. Breast Cancer Res Treat. 2010;123(3):725–31.PubMed
35.
Zurück zum Zitat Mihaly Z, et al. A meta-analysis of gene expression-based biomarkers predicting outcome after tamoxifen treatment in breast cancer. Breast Cancer Res Treat. 2013;140(2):219–32.PubMed Mihaly Z, et al. A meta-analysis of gene expression-based biomarkers predicting outcome after tamoxifen treatment in breast cancer. Breast Cancer Res Treat. 2013;140(2):219–32.PubMed
36.
Zurück zum Zitat Chandrashekar DS, et al. UALCAN: a portal for facilitating tumor subgroup gene expression and survival analyses. Neoplasia. 2017;19(8):649–58.PubMedPubMedCentral Chandrashekar DS, et al. UALCAN: a portal for facilitating tumor subgroup gene expression and survival analyses. Neoplasia. 2017;19(8):649–58.PubMedPubMedCentral
37.
Zurück zum Zitat Tai CJ, et al. Increased cellular apoptosis susceptibility (CSE1L/CAS) protein expression promotes protrusion extension and enhances migration of MCF-7 breast cancer cells. Exp Cell Res. 2010;316(17):2969–81.PubMed Tai CJ, et al. Increased cellular apoptosis susceptibility (CSE1L/CAS) protein expression promotes protrusion extension and enhances migration of MCF-7 breast cancer cells. Exp Cell Res. 2010;316(17):2969–81.PubMed
38.
Zurück zum Zitat Monian P, Jiang X. The cellular apoptosis susceptibility protein (CAS) promotes tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis and cell proliferation. J Biol Chem. 2016;291(5):2379–88.PubMed Monian P, Jiang X. The cellular apoptosis susceptibility protein (CAS) promotes tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis and cell proliferation. J Biol Chem. 2016;291(5):2379–88.PubMed
39.
Zurück zum Zitat Baldin V, et al. Cyclin D1 is a nuclear protein required for cell cycle progression in G1. Genes Dev. 1993;7(5):812–21.PubMed Baldin V, et al. Cyclin D1 is a nuclear protein required for cell cycle progression in G1. Genes Dev. 1993;7(5):812–21.PubMed
40.
Zurück zum Zitat Shivakumar L, et al. The RASSF1A tumor suppressor blocks cell cycle progression and inhibits cyclin D1 accumulation. Mol Cell Biol. 2002;22(12):4309–18.PubMedPubMedCentral Shivakumar L, et al. The RASSF1A tumor suppressor blocks cell cycle progression and inhibits cyclin D1 accumulation. Mol Cell Biol. 2002;22(12):4309–18.PubMedPubMedCentral
41.
Zurück zum Zitat Behrens P, et al. Implication of the proliferation and apoptosis associated CSE1L/CAS gene for breast cancer development. Anticancer Res. 2001;21(4A):2413–7.PubMed Behrens P, et al. Implication of the proliferation and apoptosis associated CSE1L/CAS gene for breast cancer development. Anticancer Res. 2001;21(4A):2413–7.PubMed
42.
Zurück zum Zitat Tung MC, et al. Higher prevalence of secretory CSE1L/CAS in sera of patients with metastatic cancer. Cancer Epidemiol Biomarkers Prev. 2009;18(5):1570–7.PubMed Tung MC, et al. Higher prevalence of secretory CSE1L/CAS in sera of patients with metastatic cancer. Cancer Epidemiol Biomarkers Prev. 2009;18(5):1570–7.PubMed
43.
Zurück zum Zitat Ma S, et al. LncRNA BANCR promotes tumorigenesis and enhances adriamycin resistance in colorectal cancer. Aging. 2018;10(8):2062–78.PubMedPubMedCentral Ma S, et al. LncRNA BANCR promotes tumorigenesis and enhances adriamycin resistance in colorectal cancer. Aging. 2018;10(8):2062–78.PubMedPubMedCentral
44.
Zurück zum Zitat Luo W, et al. 24-Hydroxylase in cancer: impact on vitamin D-based anticancer therapeutics. J Steroid Biochem Mol Biol. 2013;136:252–7.PubMed Luo W, et al. 24-Hydroxylase in cancer: impact on vitamin D-based anticancer therapeutics. J Steroid Biochem Mol Biol. 2013;136:252–7.PubMed
45.
Zurück zum Zitat Horvath HC, et al. The candidate oncogene CYP24A1: a potential biomarker for colorectal tumorigenesis. J Histochem Cytochem. 2010;58(3):277–85.PubMedPubMedCentral Horvath HC, et al. The candidate oncogene CYP24A1: a potential biomarker for colorectal tumorigenesis. J Histochem Cytochem. 2010;58(3):277–85.PubMedPubMedCentral
46.
Zurück zum Zitat Chen G, et al. CYP24A1 is an independent prognostic marker of survival in patients with lung adenocarcinoma. Clin Cancer Res. 2011;17(4):817–26.PubMed Chen G, et al. CYP24A1 is an independent prognostic marker of survival in patients with lung adenocarcinoma. Clin Cancer Res. 2011;17(4):817–26.PubMed
47.
Zurück zum Zitat Sakakia T, et al. CYP24A1 as a potential target for cancer therapy. Anticancer Agents Med Chem. 2014;14:97–108. Sakakia T, et al. CYP24A1 as a potential target for cancer therapy. Anticancer Agents Med Chem. 2014;14:97–108.
48.
Zurück zum Zitat Zhalehjoo N, Shakiba Y, Panjehpour M. Gene expression profiles of CYP24A1 and CYP27B1 in malignant and normal breast tissues. Mol Med Rep. 2017;15(1):467–73.PubMed Zhalehjoo N, Shakiba Y, Panjehpour M. Gene expression profiles of CYP24A1 and CYP27B1 in malignant and normal breast tissues. Mol Med Rep. 2017;15(1):467–73.PubMed
49.
Zurück zum Zitat Osanai M, Lee GH. CYP24A1-induced vitamin D insufficiency promotes breast cancer growth. Oncol Rep. 2016;36(5):2755–62.PubMed Osanai M, Lee GH. CYP24A1-induced vitamin D insufficiency promotes breast cancer growth. Oncol Rep. 2016;36(5):2755–62.PubMed
50.
Zurück zum Zitat Tourigny A, et al. CYP24A1 exacerbated activity during diabetes contributes to kidney tubular apoptosis via caspase-3 increased expression and activation. PLoS ONE. 2012;7(10):e48652.PubMedPubMedCentral Tourigny A, et al. CYP24A1 exacerbated activity during diabetes contributes to kidney tubular apoptosis via caspase-3 increased expression and activation. PLoS ONE. 2012;7(10):e48652.PubMedPubMedCentral
51.
Zurück zum Zitat Mohri T, et al. MicroRNA regulates human vitamin D receptor. Int J Cancer. 2009;125(6):1328–33.PubMed Mohri T, et al. MicroRNA regulates human vitamin D receptor. Int J Cancer. 2009;125(6):1328–33.PubMed
Metadaten
Titel
Cellular apoptosis susceptibility protein (CAS) suppresses the proliferation of breast cancer cells by upregulated cyp24a1
verfasst von
Mei Ye
Ruigang Han
Jianwu Shi
Xunda Wang
Allan Z. Zhao
Fanghong Li
Hao Chen
Publikationsdatum
01.05.2020
Verlag
Springer US
Erschienen in
Medical Oncology / Ausgabe 5/2020
Print ISSN: 1357-0560
Elektronische ISSN: 1559-131X
DOI
https://doi.org/10.1007/s12032-020-01366-w

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