Introduction
Sex chromosome abnormalities (SCAs) - Turner syndrome (TS [45,X]), Klinefelter syndrome (KS [47,XXY]), Triple X syndrome (Triple X, [47,XXX]) and Double Y syndrome (Double Y,[47,XYY]) - are estimated to affect approximately 1 per 400 births [
1]. A number of representative cytogenetic surveys were conducted years ago on newborns in various countries. Previously, we pooled data from these surveys in order to estimate the prevalence of TS, KS, Triple X and Double Y [
2‐
5] which were; TS: 50 per 100,000 newborn females [24 TS among 48,744 newborn females] [
6‐
10]; KS: 152 per 100,000 newborn males [84 KS among 55,212 newborn males] [
6,
7,
10‐
13]; Triple X: 84 per 100,000 newborn females [62 Triple X among 73,990 newborn females] [
4]; 4) Double Y: 98 per 100,000 newborn males [51 Double Y among 52,004 newborn males] [
7,
10,
12,
14,
15]. Estimates are, however, subject to much uncertainty.
The presence of a SCA may affect individuals at multiple organ levels, but the range of affection is very wide. Abnormal and delayed puberty as well as infertility are, however, distinctive features in individuals affected by TS or KS [
16,
17]. Previously, we and others, have reported an almost four-fold increased mortality [
3‐
5,
18,
19] as well as increased morbidity associated to a wide range of diseases [
18,
20] in individuals affected by SCAs compared to age and sex-matched controls. Further, we have reported a reduced socioeconomic status in individuals affected by a SCA with lower education (except for TS), increased risk of being retired from the labor market, lower income, and reduced likelihood of living in a relationship [
21‐
24].
Delayed or even non-diagnosis of SCAs is common. Individuals not diagnosed in infancy often do not receive a diagnosis until years after relevant medical therapy should have been initiated to alleviate symptoms [
4,
5,
25‐
27]. Further, delayed diagnosis prevents timely screening and intervention for common associated health problems as well as learning and behavioral disabilities [
28,
29]. Thus, health related and social consequences of non-diagnosis and delayed diagnosis are a continuous concern.
In the present study we aimed to investigate change over time in incidence, prevalence, and age at diagnosis in a national cohorts of SCAs.
Material and methods
Registries and cases
The Danish Civil Registration System was established in 1968 and since then all persons residing in Denmark have been assigned a unique 10-digit civil personal registration (CPR) number. The last digit in the CPR number allows identification of the persons’ officially registered sex (odd = male; even = female). Further, the CPR number allows accurate matching of data from different data sources [
30]. The Danish health care system is a public tax funded system ensuring all citizens free and equal health care access.
The Danish Cytogenetic Central Registry (DCCR) was established in 1968, and holds data on all pre-and postnatal karyotypes performed in Denmark since 1961. Only postnatally diagnosed individuals or prenatally diagnosed individuals, subsequently postnatally confirmed, are included in the present study. Data in the DCCR includes: 1) CPR number; 2) karyotype; 3) date of birth; and 4) date of karyotyping. The DCCR holds no information on phenotype or reasons for performing a karyotype. Information concerning degrees of mosaicism is not available.
The DCCR was searched for all cases diagnosed with a karyotype compatible with TS, KS, Triple X or Double Y during 1961–2014. The distribution of accepted karyotypes for each syndrome are presented in Table
1. We considered females with a 45,X mosaic composition to have a phenotype most consistent with TS and these cases were thus included in the TS group. Females with more than three X chromosomes (e.g. 48,XXXX) and males with more than two Y chromosomes (e.g. 48,XYYY) were included in the Triple X and Double Y group, respectively. Cases with an autosomal aneuploidy were included in their respective group of SCA. Data were retrieved from the DCCR in October 2015.
Table 1
Distribution of karyotypes among Turner syndrome, Klinefelter syndrome, Triple X and Double Y syndrome
Turner syndrome | 1156 |
45,X | 422 |
45,X/46,XX | 287 |
Karyotypes containing an isochromosome: 45,X/46,I(X) and equivalents | 117 |
Karyotypes containing Y chromosome material: 45,X/46,XY; and equivalents | 47 |
Other | 283 |
Klinefelter syndrome | 1235 |
47,XXY | 1080 |
47,XXY/46,XY | 78 |
47,XXY/46,XX/46,XY; 46,XY/47,XXY/48,XXXY; and equivalents | 32 |
Karyotypes with an autosomal aneuplodi: 48,XXY,+ 18; 48,XXY,+ 21 | 5 |
Other | 40 |
Triple X syndrome | 197 |
47,XXX | 104 |
47,XXX/46,XX | 58 |
48,XXXX; 49,XXXXX | 10 |
Karyotypes with an autosomal aneuplodi: 48,XXX + 18; 48,XXX + 21; and quivalents | 10 |
Other | 15 |
Double Y syndrome | 287 |
47,XYY | 206 |
47,XYY/46,XY | 26 |
Karyotypes containing an isochromosome: 46,XY/47,XY,+I(Yq); 45,X/47,XY,+I(Y)/46,XY; and equivalents | 6 |
48,XXYY; 48,XYYY; and equivalents | 35 |
Other | 14 |
The Causes of Death Registry holds information on all deaths since 1970 including date of death and primary and auxiliary cause of death [
31]. We retrieved data on all deceased cases during 1970 to December 31, 2014. Further, from the Civil Registration system complete data on migrations in and out of Denmark were retrieved.
Statistics Denmark is a state institution under the Ministry of Economic Affairs and the Interior and collects data on the Danish population. From Statistics Denmark (
https://www.dst.dk/en), annually data concerning the number of females and males living in Denmark were retrieved. Likewise was annual data on the Danish birth cohorts.
Statistics
We distinguish between population-based prevalence and prevalence among newborns. In the following, we solely use the term “prevalence” when describing the prevalence of SCAs among newborns. All cases were incident the year they were diagnosed and prevalent the year they were born.
The population-based prevalence was estimated as the annual number of cases being alive in Denmark each year during the study period (1970–2014). 1970 was chosen as start of the observation period to avoid confounding from a run-in phase of the DCCR. We defined cases diagnosed prior to 1970 to be prevalent in 1970. Deceased cases were excluded the first year following death. Emigrated cases were excluded the first year following emigration, if emigration was not followed by subsequent immigration. A population-based prevalence was also estimated using the expected prevalence of the individual SCAs. Linear extrapolation were used to estimate when the observed and the expected population-based prevalence equals each other.
Incidence was estimated as the average number of diagnosed cases per million females (TS and Triple X) or males (KS and Double Y) in the background population each year during the study period. Cases diagnosed prior to 1970 were not included in this analysis.
Prevalence was estimated as the average number of cases being born per 100,000 newborn females (TS and Triple X) or males (KS and Double Y) in the background population. Cases were clustered according to 5-year calendar time periods, and the number of diagnosed cases per 5 years was divided by the sum of their five respective birth cohorts. Data on the Danish birth cohorts are available since 1901, thus observation periods started in 1901, and cases born prior to 1901 (TS: n = 5; KS: n = 11; Triple X: n = 2; and Double Y: n = 1) were thus not included in this analysis. All observation periods ended in 2014.
Time trend in incidence was analyzed using Poisson regression. Time trend in age at diagnosis during the study period was analyzed using linear regression. Differences in age at diagnosis among subgroups within one syndrome or among different syndromes were analyzed using Kruskal-Wallis test. P-values < 0.05 was considered statistically significant. Analyzes were made in StataCorp 13.1 and 15.1 for Windows.
Discussion
This population based study shows that the previously estimated prevalence of 50 TS per 100,000 may be an underestimate as we here present data showing a prevalence of 59 TS per 100,000 newborn females, corresponding to 1 per 1700. The karyotypic composition of the TS population is seemingly changing as the incidence of 45,X TS is decreasing and the incidence of TS with other karyotypes is increasing. Among KS and Double Y incidence is increasing as well, whereas it is stable among Triple X. Non-diagnosis remains extensive among KS, Triple X and Double Y. All TS eventually become diagnosed, although with a considerable delay.
The prevalence of the four forms of SCAs varied with a similar pattern during the study period. The marked increase in prevalence observed during the 1960’ties to the 1990’ties, possibly reflects that affected individuals from these birth cohorts were either born or reached puberty or fertil age at a time when karyotyping had become an established diagnostic procedure. However, comparing the maxiumum average prevalence with the expected prevalence of SCAs, the majority of KS (62%), Triple X (87%) and Double Y (82%) remain undiagnosed. In 2003, we reported that approximately 75% of expected KS was undiagnosed [
2]. Although more with KS are diagnosed presently, we consider this far from satisfactory. In contrast, the average prevalence of TS exceeded the expected prevalence.
The population-based prevalence was steadily increasing for all SCAs during the study period caused by the build-up of the DCCR with continuing recruitment exceeding the rate of exit, a phenomenon known from other rare conditions as well [
32]. The population-based prevalence will be stable when recruitment equals exit (death or emigration). Owing to increased mortality as well as increased rates of induced abortions among SCAs [
33,
34] it will though remain lower than expected even with complete diagnosis of SCAs. However, it is difficult to ascertain how much prevalence is affected hereby. Only approximately 5% of pregnant women in Denmark have a prenatal karyotype performed [
35], but they are of course selected as high-risk patients based on triple screening and non-invasive prenatal testing.
The incidence among all TS was stable during the study period. Interestingly, the incidence was steeply decreasing for 45,X TS and increasing for TS with other karyotypes. If this development continues it seems likely that 45,X TS are becoming extinct. Between 2004 and 2006, Denmark instituted a free prenatal screening program for Down syndrome (DS), in which over 90% of pregnant women in Denmark participate, and previously we have reported that approximately 40% of expected TS fetuses are detected by the DS screening [
34]. The high induced abortion rate among prenatally detected 45,X TS fetuses likely contribute to the decreasing incidence of 45,X TS. Induced abortion among TS fetuses with other karyotypes are less pronounced [
34], and a generally more favorable phenotype among these TS [
36,
37] may prevent early diagnosis. A likely explanation for the increase in incidence among TS with other karyotypes may be that fertility treatment has become more common and thus more are diagnosed owing to fertility problems.
Late diagnosis as well as non-diagnosis of SCAs have been a continuous concern [
25‐
27,
38] since SCAs are associated with increased morbidity and mortality [
3,
5,
20,
23,
39], learning and/or behavioral disabilities [
40‐
45] as well as reduced socioeconomic outcomes. Although there is lack of evidence regarding the influence of age at diagnosis on long-term outcomes, we believe that early diagnosis will provide better overall long-term outcome in SCAs by providing an opportunity for timely intervention against associated health problems as well as against learning and behavioral problems. Regrettably, the present study shows that delay in diagnosis remains a major problem (Fig.
5). Increased vigilance among health care professionals as well as a more liberal access to diagnostic tools was however hypothesized as having contributed to a decreasing diagnostic delay. As previously suggested for both TS and KS, neonatal screening programs would allow complete diagnosis of SCAs without diagnostic delay. Population-based, neonatal screening can be considered for conditions which have: 1) the magnitude to be an important health problem with a latent and early asymptomatic stage; and 2) has a well-understood natural history for which there are accepted treatments with associated facilities for diagnoses and treatment [
46]. We consider these requirements fulfilled for SCAs. Due to the rarity of the syndromes it will, however, take a long time to demonstrate associations between early diagnosis, continuous specialized care and improved long-term outcomes.
The broad clinical spectrum observed among SCAs, ranging from overt to minimal or no apparent clinical features and only subtle symptoms [
17,
47,
48] likely relates to the non-diagnosis and the delayed diagnosis. The fate of individuals with SCAs escaping diagnosis remains an enigma. Possibly, undiagnosed individuals experience a spectrum of similar challenges as those diagnosed, yet they might remain undiagnosed due to reluctance of seeking medical advice or due to lack of attention from health professionals. Based on our clinical experience, we believe that individuals diagnosed late in life more or less struggle with similar problems as those being diagnosed early in life. Recent data from UK among TS and Triple X support this hypothesis [
36]. Here a large number of presumably undiagnosed 45,X and 47,XXX females were detected approximately about 250,000 examined females, presenting with typical features for both of these syndromes.
Conclusions
The karyotypic composition of TS is changing with less 45,X and more mosaic TS being diagnosed, possibly due to increased frequency of legal abortions among 45,X TS. TS have a higher prevalence than previously anticipated as observed in 1 per 1700 newborn females. The majority of KS, Triple X and Double Y remain undiagnosed despite an increase in diagnostic activity among KS and Double Y. Delayed diagnosis is a continuous problem among all SCAs, and no change over time has been observed. We consider a newborn screening program as the only opportunity to reduce both diagnostic delay as well as non-diagnosis and with a potential to improve health and socioeconomics among individuals affected by SCAs.
Acknowledgments
Data manager, Jan Hansen, from the DCCR is sincerely thanked for identifying patients in the DCCR.