Erschienen in:
26.05.2016 | Original Article
Characterisation of a novel enterobacteria phage, CAjan, isolated from rat faeces
verfasst von:
Alexander B. Carstens, Witold Kot, Rene Lametsch, Horst Neve, Lars H. Hansen
Erschienen in:
Archives of Virology
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Ausgabe 8/2016
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Abstract
In this study, we describe the isolation and characterisation of the novel enterobacteria phage CAjan. This phage belongs to the order Caudovirales and the family Siphoviridae. The phage possesses a linear, double-stranded DNA genome consisting of 59,670 bp with a G+C content of 44.7 % and 91 predicted open reading frames (ORFs). Putative functions were assigned to 39 of the ORFs (37.4 %). The phage structural genes were furthermore functionally characterised by LC MS/MS. CAjan, together with Escherichia phage Seurat and Escherichia phage slur01, represent a novel and genetically distinct clade of Siphoviridae phages that could be considered to constitute a new phage genus. Despite limited sequence similarity, the phages in this group share a number of other common features, including genome structure and the presence of queuosine biosynthesis genes.