Background
Methods
β-lactamase producing N. gonorrhoeaeisolates
Antimicrobial susceptibility testing
β-lactamase hydrolysis assay
Plasmid DNA extraction
Genomic DNA extraction
Plasmid typing
Sequencing of the blaTEMgene
Molecular epidemiological characterisation using Neisseria gonorrhoeaemultiantigen sequence typing (NG-MAST)
Results
Antimicrobial susceptibility of β-lactamase producing N. gonorrhoeaeisolates (n = 139) from 2000 to 2011
β-lactamase plasmid types and blaTEMalleles
β-lactamase encoding gene (No. of isolates) | β-lactamase producing plasmid types (%) | Number different NG-MAST STs | Year (WHO Region) of isolation |
---|---|---|---|
bla
TEM-1 (104) | African (85.6), Asian (14.4) | 93 | 2000-2011 (All Regions
a
) |
bla
TEM-135 (30) | Rio/Toronto (86.7), Asian (10), African (3.3), | 29 | 2000-2009 (All Regions
a
) |
bla
TEM-P14S
b
(2) | African (100) | 2 | 2008 (European) |
bla
TEM-P14T
b
(1) | Asian (100) | 1 | 2003 (European) |
bla
TEM-E110K (1) | African (100) | 1 | 2003 (African) |
bla
TEM-G228S (1) | African (100) | 1 | 2009 (Western Pacific) |
bla
TEM-135
c
| Rio/Toronto | 1984 (American) | |
bla
TEM-1
c
| African | Late-1970s (American) | |
bla
TEM-1
c
| Asian | Late-1970s (American) |