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Erschienen in: Medical Microbiology and Immunology 2/2011

01.05.2011 | Original Investigation

Characterisation of the epitope for a herpes simplex virus glycoprotein B-specific monoclonal antibody with high protective capacity

verfasst von: Martin P. Däumer, Beate Schneider, Doris M. Giesen, Sheriff Aziz, Rolf Kaiser, Bernd Kupfer, Karl E. Schneweis, Jens Schneider-Mergener, Ulrich Reineke, Bertfried Matz, Anna M. Eis-Hübinger

Erschienen in: Medical Microbiology and Immunology | Ausgabe 2/2011

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Abstract

Monoclonal antibody (MAb) 2c, specific for glycoprotein B of herpes simplex virus (HSV), had been shown to mediate clearance of infection from the mucous membranes of mice, thereby completely inhibiting mucocutaneous inflammation and lethality, even in mice depleted of both CD4+ and CD8+ cells. Additionally, ganglionic infection was highly restricted. In vitro, MAb 2c exhibits a potent complement-independent neutralising activity against HSV type 1 and 2, completely inhibits the viral cell-to-cell spread as well as the syncytium formation induced by syncytial HSV strains (Eis-Hübinger et al. in Intervirology 32:351–360, 1991; Eis-Hübinger et al. in J Gen Virol 74:379–385, 1993). Here, we describe the mapping of the epitope for MAb 2c. The antibody was found to recognise a discontinuous epitope comprised of the HSV type 1 glycoprotein B residues 299 to 305 and one or more additional discontinuous regions that can be mimicked by the sequence FEDF. Identification of the epitope was confirmed by loss of antibody binding to mutated glycoprotein B with replacement of the epitopic key residues, expressed in COS-1 cells. Similarly, MAb 2c was not able to neutralise HSV mutants with altered key residues, and MAb 2c was ineffective in mice inoculated with such mutants. Interestingly, identification and fine-mapping of the discontinuous epitope was not achieved by binding studies with truncated glycoprotein B variants expressed in COS cells but by peptide scanning with synthetic overlapping peptides and peptide key motif analysis. Reactivity of MAb 2c was immensely increased towards a peptide composed of the glycoprotein B residues 299 to 305, a glycine linker, and a C-terminal FEDF motif. If it could be demonstrated that antibodies of the specificity and bioactivity of MAb 2c can be induced by the epitope or a peptide mimicking the epitope, strategies for active immunisation might be conceivable.
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Metadaten
Titel
Characterisation of the epitope for a herpes simplex virus glycoprotein B-specific monoclonal antibody with high protective capacity
verfasst von
Martin P. Däumer
Beate Schneider
Doris M. Giesen
Sheriff Aziz
Rolf Kaiser
Bernd Kupfer
Karl E. Schneweis
Jens Schneider-Mergener
Ulrich Reineke
Bertfried Matz
Anna M. Eis-Hübinger
Publikationsdatum
01.05.2011
Verlag
Springer-Verlag
Erschienen in
Medical Microbiology and Immunology / Ausgabe 2/2011
Print ISSN: 0300-8584
Elektronische ISSN: 1432-1831
DOI
https://doi.org/10.1007/s00430-010-0174-x

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