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Erschienen in: Archives of Virology 4/2017

19.12.2016 | Annotated Sequence Record

Characterization and complete genome sequence of a panicovirus from Bermuda grass by high-throughput sequencing

verfasst von: Muhammad N. Tahir, Ben Lockhart, Samuel Grinstead, Dimitre Mollov

Erschienen in: Archives of Virology | Ausgabe 4/2017

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Abstract

Bermuda grass samples were examined by transmission electron microscopy and 28-30 nm spherical virus particles were observed. Total RNA from these plants was subjected to high-throughput sequencing (HTS). The nearly full genome sequence of a panicovirus was identified from one HTS scaffold. Sanger sequencing was used to confirm the HTS results and complete the genome sequence of 4404 nt. This virus was provisionally named Bermuda grass latent virus (BGLV). Its predicted open reading frames follow the typical arrangement of the genus Panicovirus. Based on sequence comparisons and phylogenetic analyses BGLV differs from other viruses and therefore taxonomically it is a new member of the genus Panicovirus, family Tombusviridae.
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Metadaten
Titel
Characterization and complete genome sequence of a panicovirus from Bermuda grass by high-throughput sequencing
verfasst von
Muhammad N. Tahir
Ben Lockhart
Samuel Grinstead
Dimitre Mollov
Publikationsdatum
19.12.2016
Verlag
Springer Vienna
Erschienen in
Archives of Virology / Ausgabe 4/2017
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-016-3165-4

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