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Erschienen in: Journal of Parasitic Diseases 4/2017

17.07.2017 | Original Article

Characterization of a new isolate of entomopathogenic nematode, Steinernema sangi (Rhabditida, Steinernematidae), and its symbiotic bacteria Xenorhabdus vietnamensis (γ-Proteobacteria) from Mizoram, northeastern India

verfasst von: H. C. Lalramnghaki, Vanlalhlimpuia, Vanramliana, Lalramliana

Erschienen in: Journal of Parasitic Diseases | Ausgabe 4/2017

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Abstract

A survey was conducted to isolate indigenous EPN, specifically from the northeastern part of India, a biodiversity hotspot region, to record the occurrence and their further use as biological control agent. The morphological and molecular analysis (ITS rDNA for Steinernema and 16S rRNA for Xenorhabdus) revealed that the entomopathogenic nematodes isolated from four different habitats and its symbiotic bacteria are conspecific with Steinernema sangi and Xenorhabdus vietnamensis respectively. The phylogenetic analysis based on maximum parsimony (MP) revealed that Steinernema sangi belongs to feltiae-kraussei-oregonense group. The study constitutes the first report of Steinernema sangi and its symbiotic bacteria Xenorhabdus vietnamensis outside the type locality, Vietnam, and in particular from India.
Literatur
Zurück zum Zitat Ali SS, Shaheen A (2011) Steinernema sayeedae sp. n. a heat tolerant EPN from banana Rhizosphere of Koshambhi District, U.P, India. Trends Biosci 4:123–125 Ali SS, Shaheen A (2011) Steinernema sayeedae sp. n. a heat tolerant EPN from banana Rhizosphere of Koshambhi District, U.P, India. Trends Biosci 4:123–125
Zurück zum Zitat Ali SS, Shaheen A, Pervez R, Hussain MA (2005) Steinernema masoodi sp. n. and Steinernema seemae sp. n. (Rhabditida: Steinernematidae). Int J Nematol 15:89–99 Ali SS, Shaheen A, Pervez R, Hussain MA (2005) Steinernema masoodi sp. n. and Steinernema seemae sp. n. (Rhabditida: Steinernematidae). Int J Nematol 15:89–99
Zurück zum Zitat Ali SS, Shaheen A, Asif M, Akhtar MH (2009) Steinernema qazii sp. n. (Nematoda: Rhabditidae: Steinernematidae) from Kanpur, India. Trends Biosci 2:59–64 Ali SS, Shaheen A, Asif M, Akhtar MH (2009) Steinernema qazii sp. n. (Nematoda: Rhabditidae: Steinernematidae) from Kanpur, India. Trends Biosci 2:59–64
Zurück zum Zitat Bhat AH, Istkhar Chaubey AK, Půža V, San-Blas E (2017) First report and comparative study of Steinernema surkhetense (Rhabditida: Steinernematidae) and its symbiont bacteria from subcontinental India. J Nematol 49:92–102CrossRefPubMedPubMedCentral Bhat AH, Istkhar Chaubey AK, Půža V, San-Blas E (2017) First report and comparative study of Steinernema surkhetense (Rhabditida: Steinernematidae) and its symbiont bacteria from subcontinental India. J Nematol 49:92–102CrossRefPubMedPubMedCentral
Zurück zum Zitat Campos-Herrera R, Barbercheck M, Hoy CW, Stock SP (2012) Entomopathogenic nematodes as a model system for advancing the frontiers of ecology. J Nematol 44:162–176PubMedPubMedCentral Campos-Herrera R, Barbercheck M, Hoy CW, Stock SP (2012) Entomopathogenic nematodes as a model system for advancing the frontiers of ecology. J Nematol 44:162–176PubMedPubMedCentral
Zurück zum Zitat Devi G, Mishra H, Bhattacharyya B, Nath DJ (2016) Occurrence of entomopathogenic nematode (Rhabditida: Heterorhabditidae, Steinernematidae) in white grub infested areas of Majuli, Assam, India. J Biopest 9:148–156 Devi G, Mishra H, Bhattacharyya B, Nath DJ (2016) Occurrence of entomopathogenic nematode (Rhabditida: Heterorhabditidae, Steinernematidae) in white grub infested areas of Majuli, Assam, India. J Biopest 9:148–156
Zurück zum Zitat Ganguly S, Singh LK (2000) Steinernema thermophilum sp. n. (Rhabditida: Steinernematidae) from India. Int J Nematol 10:183–191 Ganguly S, Singh LK (2000) Steinernema thermophilum sp. n. (Rhabditida: Steinernematidae) from India. Int J Nematol 10:183–191
Zurück zum Zitat Ganguly S, Singh M, Lal M, Singh LK, Vyas RV, Patel DJ (2002) New record of an entomopathogenic nematode, Steinernema riobrave Cabanillas, Poinar and Raulston, 1994 from Gujarat, India. Indian J Nematol 32:223 Ganguly S, Singh M, Lal M, Singh LK, Vyas RV, Patel DJ (2002) New record of an entomopathogenic nematode, Steinernema riobrave Cabanillas, Poinar and Raulston, 1994 from Gujarat, India. Indian J Nematol 32:223
Zurück zum Zitat Ganguly S, Rathore KS, Sushil K, Singh M (2011) Steinernema meghalayensis sp. n. (Rhabditida:Steinernematidae) from northeastern hilly region of India. Indian J Nematol 41:83–97 Ganguly S, Rathore KS, Sushil K, Singh M (2011) Steinernema meghalayensis sp. n. (Rhabditida:Steinernematidae) from northeastern hilly region of India. Indian J Nematol 41:83–97
Zurück zum Zitat Hazir S, Kaya HK, Stock SP, Keskin N (2003) Entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) for biological control of soil pests. Turk J Biol 27:181–202 Hazir S, Kaya HK, Stock SP, Keskin N (2003) Entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) for biological control of soil pests. Turk J Biol 27:181–202
Zurück zum Zitat Hunt DJ (2007) Overview of taxonomy and systematics. In: Nguyen KB, Hunt DJ (eds) Entomopathogenic nematodes: systematics, phylogeny and bacterial symbionts. Nematology Monographs and Perspectives 5. Brill Publishing, Leiden, pp 27–57. doi:10.1163/ej.9789004152939.i-816.21 CrossRef Hunt DJ (2007) Overview of taxonomy and systematics. In: Nguyen KB, Hunt DJ (eds) Entomopathogenic nematodes: systematics, phylogeny and bacterial symbionts. Nematology Monographs and Perspectives 5. Brill Publishing, Leiden, pp 27–57. doi:10.​1163/​ej.​9789004152939.​i-816.​21 CrossRef
Zurück zum Zitat Hunt DJ, Subbotin SA (2016) Taxonomy and systematics. In: Hunt DJ, Nguyen KB (eds) Advances in entomopathogenic nematode taxonomy and phylogeny. Nematology monographs and perspectives 12. Brill Publishing, Leiden, pp 13–58. doi:10.1163/9789004285347_003 Hunt DJ, Subbotin SA (2016) Taxonomy and systematics. In: Hunt DJ, Nguyen KB (eds) Advances in entomopathogenic nematode taxonomy and phylogeny. Nematology monographs and perspectives 12. Brill Publishing, Leiden, pp 13–58. doi:10.​1163/​9789004285347_​003
Zurück zum Zitat Hussaini SS, Ansari MA, Ahmad W, Subbotin SA (2001) Identification of some Indian populations of Steinernema species (Nematoda) by RFLP analysis of ITS region of rDNA. Int J Nematol 11:73–76 Hussaini SS, Ansari MA, Ahmad W, Subbotin SA (2001) Identification of some Indian populations of Steinernema species (Nematoda) by RFLP analysis of ITS region of rDNA. Int J Nematol 11:73–76
Zurück zum Zitat Joyce SA, Reid A, Driver F, Curran J (1994) Application of polymerase chain reaction (PCR) methods to the identification of entomopathogenic nematodes. In: Burnell AM, Ehlers RU, Masson JP (eds) Proceeding of symposium and workshop, St. Patrick’s College, Maynooth, Co. Kildare, Ireland. European Commission, DGXII, Luxembourg, pp 178–187 Joyce SA, Reid A, Driver F, Curran J (1994) Application of polymerase chain reaction (PCR) methods to the identification of entomopathogenic nematodes. In: Burnell AM, Ehlers RU, Masson JP (eds) Proceeding of symposium and workshop, St. Patrick’s College, Maynooth, Co. Kildare, Ireland. European Commission, DGXII, Luxembourg, pp 178–187
Zurück zum Zitat Kaya HK, Stock SP (1997) Techniques in insect nematology. In: Lacey LA (ed) Manual of techniques in insect pathology. Academic Press, San Diego, pp 281–324CrossRef Kaya HK, Stock SP (1997) Techniques in insect nematology. In: Lacey LA (ed) Manual of techniques in insect pathology. Academic Press, San Diego, pp 281–324CrossRef
Zurück zum Zitat Kaya HK, Aguillera MM, Alumai A, Choo HY, de la Torre M, Fodor A, Ganguly S, Hazir S, Lakatos T, Pye A, Wilson M, Yamanaka S, Yang H, Ehlers RU (2006) Status of entomopathogenic nematodes and their symbiotic bacteria from selected countries or regions of the world. Biol Contr 38:134–155. doi:10.1016/j.biocontrol.2005.11.004 CrossRef Kaya HK, Aguillera MM, Alumai A, Choo HY, de la Torre M, Fodor A, Ganguly S, Hazir S, Lakatos T, Pye A, Wilson M, Yamanaka S, Yang H, Ehlers RU (2006) Status of entomopathogenic nematodes and their symbiotic bacteria from selected countries or regions of the world. Biol Contr 38:134–155. doi:10.​1016/​j.​biocontrol.​2005.​11.​004 CrossRef
Zurück zum Zitat Kerry BR, Hominick WM (2002) Biological control. In: Lee DL (ed) The biology of nematodes. Taylor & Francis, London, pp 483–510CrossRef Kerry BR, Hominick WM (2002) Biological control. In: Lee DL (ed) The biology of nematodes. Taylor & Francis, London, pp 483–510CrossRef
Zurück zum Zitat Kulkarni N, Rizvi AN, Kumar V, Paunikar S, Mishra VK (2012) Morphological and molecular characterization of Steinernema dharanaii sp. n.: a new entomopathogenic nematode from India. Indian J Trop Biodiv 20:107–116 Kulkarni N, Rizvi AN, Kumar V, Paunikar S, Mishra VK (2012) Morphological and molecular characterization of Steinernema dharanaii sp. n.: a new entomopathogenic nematode from India. Indian J Trop Biodiv 20:107–116
Zurück zum Zitat Lalramliana, Yadav AK (2010) Occurrence of entomopathogenic nematodes (Rhabditida: Steinernematidae and Heterorhabditidae) in Meghalaya, NE India. Sci Vis 10:89–100 Lalramliana, Yadav AK (2010) Occurrence of entomopathogenic nematodes (Rhabditida: Steinernematidae and Heterorhabditidae) in Meghalaya, NE India. Sci Vis 10:89–100
Zurück zum Zitat Lalramliana, Yadav AK (2016) Effects of storage temperature on survival and infectivity of three indigenous entomopathogenic nematodes strains (Steinernematidae and Heterorhabditidae) from Meghalaya, India. J Parasit Dis 40:1150–1154. doi:10.1007/s12639-014-0639-8 CrossRefPubMed Lalramliana, Yadav AK (2016) Effects of storage temperature on survival and infectivity of three indigenous entomopathogenic nematodes strains (Steinernematidae and Heterorhabditidae) from Meghalaya, India. J Parasit Dis 40:1150–1154. doi:10.​1007/​s12639-014-0639-8 CrossRefPubMed
Zurück zum Zitat Lalramliana, Shylesha AN, Yadav AK (2005) Effects of temperature and relative humidity on the emergence of infective juveniles of Heterorhabditis indica from Meghalaya, India. Indian J Nematol 35:157–159 Lalramliana, Shylesha AN, Yadav AK (2005) Effects of temperature and relative humidity on the emergence of infective juveniles of Heterorhabditis indica from Meghalaya, India. Indian J Nematol 35:157–159
Zurück zum Zitat Lee MM, Stock SP (2010) A multilocus approach to assessing co-evolutionary relationships between Steinernema spp. (Nematoda: Steinernematidae) and their bacterial symbionts Xenorhabdus spp. (γ-Proteobacteria: Enterobacteriaceae). Syst Parasitol 77:1–12. doi:10.1007/s11230-010-9256-9 CrossRefPubMed Lee MM, Stock SP (2010) A multilocus approach to assessing co-evolutionary relationships between Steinernema spp. (Nematoda: Steinernematidae) and their bacterial symbionts Xenorhabdus spp. (γ-Proteobacteria: Enterobacteriaceae). Syst Parasitol 77:1–12. doi:10.​1007/​s11230-010-9256-9 CrossRefPubMed
Zurück zum Zitat Nguyen KB, Stuart RJ, Andalo V, Gozel U, Rogers ME (2007) Steinernema texanum n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Texas, USA. Nematology 9:379–396. doi:10.1163/156854107781352025 CrossRef Nguyen KB, Stuart RJ, Andalo V, Gozel U, Rogers ME (2007) Steinernema texanum n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Texas, USA. Nematology 9:379–396. doi:10.​1163/​1568541077813520​25 CrossRef
Zurück zum Zitat Nguyen KB, Půža V, Mracek Z (2008) Steinernema cholashanense n. sp. (Rhabditida, Steinernematidae) a new species of entomopathogenic nematode from the province of Sichuan, Chola Shan Mountains, China. J Invertebr Pathol 97:251–264. doi:10.1016/j.jip.2007.06.006 CrossRefPubMed Nguyen KB, Půža V, Mracek Z (2008) Steinernema cholashanense n. sp. (Rhabditida, Steinernematidae) a new species of entomopathogenic nematode from the province of Sichuan, Chola Shan Mountains, China. J Invertebr Pathol 97:251–264. doi:10.​1016/​j.​jip.​2007.​06.​006 CrossRefPubMed
Zurück zum Zitat Pervez R, Ali SS, Asif M (2009) A new species of entomopathogenic nematodes Steinernema mushtaqi sp. n. (Nematoda: Rhabditidae : Steinernematidae) from chickpea rhizosphere, In: Proceedings of international conference on legumes (ICGL), Indian Institute of Pulses Research, Kanpur, February 2009, pp 14–16 Pervez R, Ali SS, Asif M (2009) A new species of entomopathogenic nematodes Steinernema mushtaqi sp. n. (Nematoda: Rhabditidae : Steinernematidae) from chickpea rhizosphere, In: Proceedings of international conference on legumes (ICGL), Indian Institute of Pulses Research, Kanpur, February 2009, pp 14–16
Zurück zum Zitat Phan KL, Nguyen NC, Moens M (2001) Steinernema sangi sp. n. (Rhabditida: Steinernematidae) from Vietnam. Russ J Nematol 9:1–7 Phan KL, Nguyen NC, Moens M (2001) Steinernema sangi sp. n. (Rhabditida: Steinernematidae) from Vietnam. Russ J Nematol 9:1–7
Zurück zum Zitat Poinar GO Jr, Karunakar GK, David H (1992) Heterorhabditis indicus n sp. (Rhabditida: Nematoda) from India: separation of Heterorhabditis spp. by infective juveniles. Fundam Appl Nematol 15:467–472 Poinar GO Jr, Karunakar GK, David H (1992) Heterorhabditis indicus n sp. (Rhabditida: Nematoda) from India: separation of Heterorhabditis spp. by infective juveniles. Fundam Appl Nematol 15:467–472
Zurück zum Zitat Qiu L, Zhao J, Wu Z, Lv Z, Pang Y (2011) Steinernema pui sp. n. (Rhabditida, Steinernematidae), a new entomopathogenic nematode from Yunnan, China. Zootaxa 2767:1–13 Qiu L, Zhao J, Wu Z, Lv Z, Pang Y (2011) Steinernema pui sp. n. (Rhabditida, Steinernematidae), a new entomopathogenic nematode from Yunnan, China. Zootaxa 2767:1–13
Zurück zum Zitat Rolston A, Meade C, Boyle S, Kakouli-Duarte T, Downes M (2009) Intraspecific variation among isolates of the entomopathogenic nematode Steinernema feltiae from Bull Island, Ireland. Nematology 11:439–451. doi:10.1163/156854109X447015 CrossRef Rolston A, Meade C, Boyle S, Kakouli-Duarte T, Downes M (2009) Intraspecific variation among isolates of the entomopathogenic nematode Steinernema feltiae from Bull Island, Ireland. Nematology 11:439–451. doi:10.​1163/​156854109X447015​ CrossRef
Zurück zum Zitat Spiridonov SE, Reid AP, Podrucka K, Subbotin SA, Moens M (2004) Phylogenetic relationships within the genus Steinernema (Nematoda: Rhabditida) as inferred from analyses of sequences of the ITS1-5.8S-ITS2 region of rDNA and morphological features. Nematology 6:547–566. doi:10.1163/1568541042665304 CrossRef Spiridonov SE, Reid AP, Podrucka K, Subbotin SA, Moens M (2004) Phylogenetic relationships within the genus Steinernema (Nematoda: Rhabditida) as inferred from analyses of sequences of the ITS1-5.8S-ITS2 region of rDNA and morphological features. Nematology 6:547–566. doi:10.​1163/​1568541042665304​ CrossRef
Zurück zum Zitat Stock SP, Pryor BM, Kaya HK (1999) Distribution of entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) in natural habitats in California. Biodiv Conserv 8:535–549. doi:10.1023/A:1008827422372 CrossRef Stock SP, Pryor BM, Kaya HK (1999) Distribution of entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) in natural habitats in California. Biodiv Conserv 8:535–549. doi:10.​1023/​A:​1008827422372 CrossRef
Zurück zum Zitat Stock SP, Campbell JF, Nadler SA (2001) Phylogeny of Steinernema Travassos, 1927 (Cephalobina: Steinernematidae) inferred from ribosomal DNA sequences and morphological characters. J Parasitol 87:877–889. doi:10.1645/0022-3395(2001)087[0877:POSTCS]2.0.CO;2 Stock SP, Campbell JF, Nadler SA (2001) Phylogeny of Steinernema Travassos, 1927 (Cephalobina: Steinernematidae) inferred from ribosomal DNA sequences and morphological characters. J Parasitol 87:877–889. doi:10.1645/0022-3395(2001)087[0877:POSTCS]2.0.CO;2
Zurück zum Zitat Stock SP, Al-Banna L, Darwish R, Katbeh A (2008) Diversity and distribution of entomopathogenic nematodes (Nematoda: Steinernematidae, Heterorhabditidae) and their bacterial symbionts (γ-Proteobacteria: Enterobacteriaceae) in Jordan. J Invertebr Pathol 98:228–234. doi:10.1016/j.jip.2008.01.003 CrossRefPubMed Stock SP, Al-Banna L, Darwish R, Katbeh A (2008) Diversity and distribution of entomopathogenic nematodes (Nematoda: Steinernematidae, Heterorhabditidae) and their bacterial symbionts (γ-Proteobacteria: Enterobacteriaceae) in Jordan. J Invertebr Pathol 98:228–234. doi:10.​1016/​j.​jip.​2008.​01.​003 CrossRefPubMed
Zurück zum Zitat Tailliez P, Laroui C, Ginibre N, Paule A, Pages S, Boemare N (2010) Phylogeny of Photorhabdus and Xenorhabdus based on universally conserved protein-coding sequences and implications for the taxonomy of these two genera. Proposal of new taxa: X. vietnamensis sp. nov., P. luminescens subsp. caribbeanensis subsp. nov., P. luminescens subsp. hainanensis subsp. nov., P. temperata subsp. khanii subsp. nov., P. temperata subsp. tasmaniensis subsp. nov., and the reclassification of P. luminescens subsp. thracensis as P. temperata subsp. thracensis comb. nov. Int J Syst Evol Microbiol 60:1921–1937. doi:10.1099/ijs.0.014308-0 CrossRefPubMed Tailliez P, Laroui C, Ginibre N, Paule A, Pages S, Boemare N (2010) Phylogeny of Photorhabdus and Xenorhabdus based on universally conserved protein-coding sequences and implications for the taxonomy of these two genera. Proposal of new taxa: X. vietnamensis sp. nov., P. luminescens subsp. caribbeanensis subsp. nov., P. luminescens subsp. hainanensis subsp. nov., P. temperata subsp. khanii subsp. nov., P. temperata subsp. tasmaniensis subsp. nov., and the reclassification of P. luminescens subsp. thracensis as P. temperata subsp. thracensis comb. nov. Int J Syst Evol Microbiol 60:1921–1937. doi:10.​1099/​ijs.​0.​014308-0 CrossRefPubMed
Zurück zum Zitat Yadav AK, Lalramliana (2012c) Soil moisture effects on the activity of three entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) isolated from Meghalaya, India. J Parasit Dis 36:94–98. doi:10.1007/s12639-011-0076-x CrossRefPubMed Yadav AK, Lalramliana (2012c) Soil moisture effects on the activity of three entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) isolated from Meghalaya, India. J Parasit Dis 36:94–98. doi:10.​1007/​s12639-011-0076-x CrossRefPubMed
Metadaten
Titel
Characterization of a new isolate of entomopathogenic nematode, Steinernema sangi (Rhabditida, Steinernematidae), and its symbiotic bacteria Xenorhabdus vietnamensis (γ-Proteobacteria) from Mizoram, northeastern India
verfasst von
H. C. Lalramnghaki
Vanlalhlimpuia
Vanramliana
Lalramliana
Publikationsdatum
17.07.2017
Verlag
Springer India
Erschienen in
Journal of Parasitic Diseases / Ausgabe 4/2017
Print ISSN: 0971-7196
Elektronische ISSN: 0975-0703
DOI
https://doi.org/10.1007/s12639-017-0945-z

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