Background
Results
Pathogenicity analysis and RT-PCR
NDV strain | COU | HO | Year | PT | Cleavage site112–117 | GT | ICPI | F No. | HN No. |
---|---|---|---|---|---|---|---|---|---|
Clone 30 | USA | CK | 1999 | V | GRQGRL | II | NA | Y18898 | Y18898 |
B1 | USA | CK | 2000 | L | GRQGRL | II | 0.13 | AF309418 | AF309418 |
LaSota | USA | CK | 1950 | L | GRQGRL | II | 0.31 | JF950510 | JF950510 |
Mukteswar | CH | CK | 2010 | L | RRQRRF | III | NA | JF950509 | JF950509 |
Herts/33 | NL | CK | 2004 | V | RRQRRF | IV | 1.99 | AY741404 | AY741404 |
NL/152608/93 | NL | CK | 1993 | V | RRQKRF | VIIa | NA | JN986837 | JN986837 |
chicken/Kudus/018/10 | INA | CK | 2010 | V | RRQKRF | VIIa | NA | HQ697260 | HQ697260 |
chicken/Sweden/97 | SE | CK | 1997 | V | RRQRRF | VIIb | NA | GU585905 | GU585905 |
Sterna/Astr/2755/2001 | RUS | CK | 2001 | V | RRQRRF | VIIb | NA | AY865652 | AY865652 |
TW-99-175 | TW | CK | 1999 | V | RRQKRF | VIIc | 1.73 | EU604057 | EU579448 |
XJ-2/97 | CH | CK | 1997 | V | RRQKRF | VIId | 1.94 | AF458011 | AF458011 |
JS/1/97/Go | CH | GO | 1997 | V | RRQRRF | VIId | 1.84 | AF456435 | AF456429 |
BP01 | CH | PE | 1999 | V | RRQKRF | VIId | NA | JN599167 | JN599167 |
JS/5/06/Go | CH | GO | 2006 | V | RRQKRF | VIId | NA | EF211811 | EF211811 |
HA-14-07-Ch | CH | CK | 2007 | V | RRQKRF | VIId | NA | GQ245787 | GQ245787 |
JSD0812 | CH | DK | 2008 | V | RRQKRF | VIId | NA | GQ849007 | GQ849007 |
sh09 | CH | CK | 2009 | V | RRQKRF | VIId | NA | GU124591 | GU124592 |
chicken/China/JSX1/2010 | CH | CK | 2010 | V | RRQKRF | VIId | NA | JX519467 | JX519467 |
JS-12-11-Ch | CH | CK | 2011 | V | RRQKRF | VIId | NA | JQ013866 | JQ013842 |
Ch/SD672/12 | CH | CK | 2012 | V | RRQKRF | VIId | NA | KC020114 | JX914489 |
NDV/Chicken/TC/1/2011 | CH | CK | 2011 | V | RRQKRF | VIId | 1.78 | KC020314 | KC020305 |
NDV/Chicken/TC/2/2011 | CH | CK | 2011 | V | RRQKRF | VIId | 1.76 | KC020315 | KC020306 |
NDV/Chicken/TC/3/2011 | CH | CK | 2011 | V | RRQKRF | VIId | 1.73 | KC020316 | KC020307 |
NDV/Chicken/TC/4/2011 | CH | CK | 2011 | V | RRQKRF | VIId | 1.75 | KC020317 | KC020308 |
NDV/Chicken/TC/5/2011 | CH | CK | 2011 | V | RRQKRF | VIId | 1.67 | KC020318 | KC020309 |
NDV/Chicken/TC/6/2011 | CH | CK | 2011 | V | RRQKRF | VIId | 1.68 | KC020319 | KC020310 |
NDV/Chicken/TC/7/2011 | CH | CK | 2011 | V | RRQKRF | VIId | 1.67 | KC020320 | KC020311 |
NDV/Chicken/TC/8/2011 | CH | CK | 2011 | V | RRQKRF | VIId | 1.70 | KC020321 | KC020312 |
NDV/Chicken/TC/9/2011 | CH | CK | 2011 | V | RRQKRF | VIId | 1.69 | KC020322 | KC020313 |
PX2/03 | CH | DK | 2003 | V | RRQKRF | VIId | NA | EF175145 | EF175145 |
Hebei/01/2012 | CH | CK | 2012 | V | RRQKRF | VIId | 1.73 | KC542914 | KC542914 |
Shandong/02/2012 | CH | CK | 2012 | V | RRQKRF | VIId | NA | KC542913 | KC542913 |
YZCQ/Liaoning/08 | CH | CK | 2008 | V | RRQKRF | VIId | NA | FJ608351 | FJ608369 |
Beijing/01/2012 | CH | CK | 2012 | V | RRQKRF | VIId | 1.91 | KC542911 | KC542911 |
SD-02-11-Ch | CH | CK | 2011 | V | RRQKRF | VIId | NA | JQ013878 | JQ013849 |
Ch/SD754/12 | CH | CK | 2012 | V | RRQKRF | VIId | NA | JX840452 | JX840450 |
Ch/SD755/12 | CH | CK | 2012 | V | RRQKRF | VIId | NA | JX840453 | JX840451 |
Ch/SD758/12 | CH | CK | 2012 | V | RRQKRF | VIId | NA | JX840455 | JX683723 |
Ch/SD01/13 | CH | CK | 2013 | V | RRQKRF | VIId | NA | KF055273 | KF055274 |
TW/99-156B | TW | CK | 1999 | V | RRQKRF | VIIe | 1.79 | AF234031 | AF400504 |
TW-02-301 | TW | CK | 2002 | V | RRQKRF | VIIe | 1.75 | DQ898538 | EU526302 |
TW-02-302 | TW | CK | 2002 | V | RRQKRF | VIIe | 1.75 | DQ898537 | EU526303 |
TW-04-CB8 | TW | CK | 2004 | V | RRQKRF | VIIe | 1.75 | DQ898528 | AB531987 |
TW/08-02 | TW | CK | 2008 | V | RRQKRF | VIIe | NA | AB512614 | AB531988 |
Nucleotide and amino acid sequence analysis of the F gene of NDVs
strains | Amino acid residues | |||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Clone 30 | M | G | S | R | P | S | T | K | N | P | A | P | M | M | L | T | I | R | V | A | L | V | L | S | C | I | C | P | A | N | S | I | D | G | R | P | L | A | A | A | 40 | |||
B1 | . | . | . | . | . | F | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | L | . | . | . | . | . | . | . | . | F | . | . | . | ||||
LaSota | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | L | . | . | . | . | . | . | . | . | . | . | . | . | ||||
Mukteswar | . | . | P | . | S | . | . | R | I | . | V | . | L | . | . | . | . | . | I | T | . | A | . | . | V | R | Q | T | V | S | . | L | . | . | . | . | . | . | . | . | ||||
Herts/33 | . | . | . | . | S | . | . | R | I | . | V | . | P | . | . | I | . | . | I | V | . | T | . | . | . | . | Q | T | V | S | . | L | . | . | . | . | . | . | . | . | ||||
NL/152608/93 | . | . | . | K | . | . | . | R | T | . | V | . | L | . | . | I | T | . | I | M | . | I | S | . | . | . | . | L | V | S | . | L | . | . | . | . | . | . | . | . | ||||
chicken/Kudus/018/10 | . | . | . | K | . | . | . | R | I | . | V | . | L | . | . | I | T | . | I | M | . | I | . | . | Y | . | . | L | V | S | . | L | . | . | . | . | . | . | . | . | ||||
chicken/Sweden/97 | . | . | . | K | . | . | . | R | I | . | V | . | L | . | . | I | T | . | . | M | . | I | . | . | . | . | R | S | I | S | . | L | . | . | . | . | . | . | . | . | ||||
Sterna/Astr/2755/2001 | . | . | . | K | . | . | . | R | I | L | V | . | L | . | . | I | T | . | . | M | . | I | . | . | . | V | R | S | T | S | . | L | . | . | . | . | . | . | . | . | ||||
TW-99-175 | . | . | . | . | S | F | . | R | I | . | . | . | L | . | . | I | T | . | I | M | . | I | . | . | . | . | . | L | T | S | . | L | . | . | . | . | . | . | . | . | ||||
XJ-2/97 | K | R | I | L | I | T | I | M | I | R | L | T | S | L | ||||||||||||||||||||||||||||||
JS/1/97/Go | . | . | P | K | S | . | . | N | V | . | . | . | L | . | . | . | V | . | I | . | . | A | . | . | . | V | R | L | T | . | . | L | . | . | . | . | . | . | . | . | ||||
BP01 | . | . | . | K | . | . | . | R | I | . | . | . | L | . | . | I | T | . | I | M | . | I | . | G | . | . | R | . | T | S | . | L | . | . | . | . | . | . | . | . | ||||
Guangxi9/2003 | . | . | . | K | . | . | . | R | S | . | . | . | L | . | . | I | . | . | I | M | . | I | . | . | . | . | R | . | T | S | . | L | . | . | . | . | . | . | . | . | ||||
JS/9/05/Go | . | . | P | . | S | . | . | R | I | . | V | . | L | . | . | T | . | . | I | T | . | A | . | . | V | R | Q | T | V | S | . | L | . | . | . | . | . | . | . | . | ||||
HA-14-07-Ch | . | . | . | K | . | . | . | R | I | . | . | . | L | . | . | V | T | . | I | M | . | I | . | G | . | . | R | . | T | S | . | L | . | . | . | . | . | . | . | . | ||||
JSD0812 | . | . | . | K | . | . | . | R | I | . | . | . | L | . | . | I | T | . | T | M | . | I | . | G | . | . | . | . | T | S | . | L | . | . | . | . | . | . | . | . | ||||
sh09 | . | . | . | . | . | . | . | R | I | . | . | . | L | . | . | I | T | Q | I | M | . | I | . | . | . | . | . | . | T | S | . | L | . | . | . | . | . | . | . | . | ||||
chicken/China/JSX1/2010 | . | . | . | K | S | . | . | R | I | . | . | . | P | . | . | V | T | . | I | M | . | I | . | G | . | . | R | . | T | S | . | L | . | . | . | . | . | . | . | . | ||||
JS-12-11-Ch | . | . | . | K | L | . | . | R | I | . | . | . | L | . | . | I | T | . | I | I | . | I | . | . | . | . | R | . | T | S | . | L | . | . | . | . | . | . | . | . | ||||
NDV/Chicken/TC/1/2011 | . | . | . | K | . | . | . | R | I | . | . | . | P | V | . | V | T | . | . | M | . | T | . | . | . | . | R | . | T | S | . | L | . | . | . | . | . | . | . | . | ||||
NDV/Chicken/TC/2/2011 | . | . | . | K | . | . | . | R | I | . | . | . | P | V | . | V | T | . | . | M | . | T | . | . | . | . | R | . | T | S | . | L | . | . | . | . | . | . | . | . | ||||
NDV/Chicken/TC/3/2011 | . | . | . | K | . | . | . | R | I | . | . | . | P | V | . | V | T | . | . | M | . | T | . | . | . | . | R | . | T | S | . | L | . | . | . | . | . | . | . | . | ||||
NDV/Chicken/TC/4/2011 | . | . | . | K | . | . | . | R | I | . | . | . | P | V | . | V | T | . | . | M | . | T | . | . | . | . | R | . | T | S | . | L | . | . | . | . | . | . | . | . | ||||
NDV/Chicken/TC/5/2011 | . | . | . | K | . | . | . | R | I | . | . | . | P | V | . | V | T | . | . | M | . | T | . | . | . | . | R | . | T | S | . | L | . | . | . | . | . | . | . | . | ||||
NDV/Chicken/TC/6/2011 | . | . | . | K | . | . | . | R | I | . | . | . | P | V | . | V | T | . | . | M | . | T | . | . | . | . | R | . | T | S | . | L | . | . | . | . | . | . | . | . | ||||
NDV/Chicken/TC/7/2011 | . | . | . | K | . | . | . | R | I | . | . | . | P | V | . | V | T | . | . | M | . | T | . | . | . | . | R | . | T | S | . | L | . | . | . | . | . | . | . | . | ||||
NDV/Chicken/TC/8/2011 | . | . | . | K | . | . | . | R | I | . | . | . | P | V | . | V | T | . | . | M | . | T | . | . | . | . | R | . | T | S | . | L | . | . | . | . | . | . | . | . | ||||
NDV/Chicken/TC/9/2011 | . | . | . | K | . | . | . | R | I | . | . | . | P | V | . | V | T | . | . | M | . | T | . | . | . | . | R | . | T | S | . | L | . | . | . | . | . | . | . | . | ||||
PX2/03 | . | . | . | K | . | . | . | R | V | . | . | . | L | . | . | I | T | . | I | M | . | I | . | G | . | . | . | S | T | S | . | L | . | . | . | . | . | . | . | . | ||||
Chicken/China/Hebei/02/2012 | . | . | . | K | . | . | . | R | I | . | . | . | L | . | . | I | T | . | I | M | . | I | . | . | . | . | R | L | T | S | . | L | . | . | . | . | . | . | . | . | ||||
Shangdong/02/2012 | . | . | . | K | . | . | . | R | I | . | . | . | L | . | . | I | T | . | I | M | . | T | . | . | . | . | R | L | T | S | . | L | . | . | . | . | . | . | . | . | ||||
YZCQ/Liaoning/08 | . | . | . | K | . | . | . | R | I | . | . | . | L | . | . | I | T | . | I | M | . | I | . | D | . | . | R | . | T | S | . | L | . | . | . | . | . | . | . | . | ||||
Beijing/01/2012 | . | . | . | K | . | . | . | . | I | . | V | . | L | . | . | I | T | . | I | M | . | I | . | N | . | . | R | L | T | S | . | L | . | . | . | . | . | . | . | . | ||||
SD-02-11-Ch | . | . | . | K | L | . | . | R | I | . | . | . | L | . | . | I | T | . | I | M | . | T | . | . | . | . | R | L | T | S | . | L | . | . | . | . | . | . | . | . | ||||
Ch/SD672/12 | . | . | . | K | . | . | . | R | I | . | . | . | L | . | . | I | T | . | I | M | . | T | . | . | . | . | R | . | T | S | . | L | . | . | . | . | . | . | . | . | ||||
Ch/SD754/12 | . | . | . | K | . | . | . | R | I | . | . | . | L | . | . | I | T | . | I | M | . | T | . | . | . | . | R | L | T | S | . | L | . | . | . | . | . | . | . | . | ||||
Ch/SD755/12 | . | . | . | K | . | . | . | R | I | . | . | . | L | . | . | I | T | . | I | M | . | T | . | . | . | . | R | L | T | S | . | L | . | . | . | . | . | . | . | . | ||||
Ch/SD758/12 | . | . | . | K | . | . | . | R | I | . | . | . | L | . | . | I | T | . | I | M | . | T | . | . | . | . | R | L | T | S | . | L | . | . | . | . | . | . | . | . | ||||
Ch/SD01/13 | . | . | . | K | . | . | . | R | I | . | . | . | L | . | . | I | T | . | I | L | . | I | . | N | . | . | R | L | T | S | . | L | . | . | . | . | . | . | . | . | ||||
TW/99-156B | . | . | . | . | S | . | . | R | I | . | . | . | L | . | . | . | T | Q | I | M | . | T | . | . | . | . | R | L | T | S | . | L | . | . | . | . | . | . | . | . | ||||
TW-02-301 | . | . | P | . | S | . | . | R | I | . | . | . | L | . | . | I | T | . | I | M | . | T | F | . | . | . | R | L | T | S | . | L | . | . | . | . | . | . | . | . | ||||
TW-02-302 | . | . | . | . | S | . | . | R | I | . | . | . | L | . | . | I | T | . | I | M | . | T | F | . | . | . | R | L | T | S | . | L | . | . | . | . | . | . | . | . | ||||
TW-04-CB8 | . | . | P | . | S | . | . | R | I | . | . | . | L | . | . | I | T | . | I | M | . | T | . | . | . | . | R | L | T | S | . | L | . | . | . | . | . | . | . | . | ||||
TW/08-02 | . | . | P | Q | T | F | . | R | I | . | . | . | L | . | . | I | T | . | I | M | . | T | F | . | . | T | R | L | . | S | . | L | . | . | . | . | . | . | . | . | ||||
strains | Amino acid residues | |||||||||||||||||||||||||||||||||||||||||||
Clone 30 | G | I | V | V | T | G | D | K | A | V | N | I | Y | T | S | S | Q | T | G | S | I | I | V | K | L | L | P | N | L | P | K | D | K | E | A | C | A | K | A | P | 80 | |||
B1 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | ||||
LaSota | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | ||||
Mukteswar | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | . | . | . | . | . | . | . | . | . | . | ||||
Herts/33 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | . | . | . | . | . | . | . | . | . | . | ||||
NL/152608/93 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | . | . | . | . | . | . | . | . | . | . | ||||
chicken/Kudus/018/10 | . | . | . | . | . | . | . | . | . | . | . | V | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | . | . | . | . | . | . | . | . | . | . | ||||
chicken/Sweden/97 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | . | . | . | . | . | . | . | . | . | . | ||||
Sterna/Astr/2755/2001 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | . | . | . | . | . | . | . | . | . | . | ||||
TW-99-175 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | . | . | . | . | . | . | . | . | . | . | ||||
XJ-2/97 | . | . | . | . | . | . | . | . | . | . | . | V | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | R | . | . | . | . | . | . | . | . | . | ||||
JS/1/97/Go | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | . | . | . | . | . | . | . | . | . | . | ||||
BP01 | . | . | . | . | . | . | . | . | . | . | . | V | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | R | . | . | . | . | . | . | . | . | . | ||||
JS/5/06/Go | . | . | . | . | . | . | . | . | . | . | . | V | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | R | . | . | . | . | . | . | . | . | . | ||||
HA-14-07-Ch | . | . | . | . | . | . | . | . | . | . | . | V | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | R | . | . | . | . | . | . | . | . | . | ||||
JSD0812 | . | . | . | . | . | . | . | . | . | . | . | V | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | R | . | . | . | . | . | . | . | . | . | ||||
sh09 | . | . | . | . | . | . | . | . | . | . | . | V | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | R | . | . | . | . | . | . | . | . | . | ||||
chicken/China/JSX1/2010 | . | . | . | . | . | . | . | . | . | . | . | V | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | R | . | . | . | . | . | . | . | . | . | ||||
JS-12-11-Ch | . | . | . | . | . | . | . | . | . | . | . | V | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | R | . | . | . | . | . | . | . | . | . | ||||
NDV/Chicken/TC/1/2011 | . | . | . | . | . | . | . | . | . | . | S | V | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | R | . | . | . | . | . | . | . | . | . | ||||
NDV/Chicken/TC/2/2011 | . | . | . | . | . | . | . | . | . | . | S | V | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | R | . | . | . | . | . | . | . | . | . | ||||
NDV/Chicken/TC/3/2011 | . | . | . | . | . | . | . | . | . | . | S | V | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | R | . | . | . | . | . | . | . | . | . | ||||
NDV/Chicken/TC/4/2011 | . | . | . | . | . | . | . | . | . | . | S | V | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | R | . | . | . | . | . | . | . | . | . | ||||
NDV/Chicken/TC/5/2011 | . | . | . | . | . | . | . | . | . | . | S | V | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | R | . | . | . | . | . | . | . | . | . | ||||
NDV/Chicken/TC/6/2011 | . | . | . | . | . | . | . | . | . | . | S | V | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | R | . | . | . | . | . | . | . | . | . | ||||
NDV/Chicken/TC/7/2011 | . | . | . | . | . | . | . | . | . | . | S | V | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | R | . | . | . | . | . | . | . | . | . | ||||
NDV/Chicken/TC/8/2011 | . | . | . | . | . | . | . | . | . | . | S | V | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | R | . | . | . | . | . | . | . | . | . | ||||
NDV/Chicken/TC/9/2011 | . | . | . | . | . | . | . | . | . | . | S | V | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | R | . | . | . | . | . | . | . | . | . | ||||
PX2/03 | . | . | . | . | . | . | . | . | . | . | . | V | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | R | . | . | . | . | . | . | . | . | . | . | |||
Chicken/China/Hebei/02/2012 | . | . | . | . | . | . | . | . | . | . | . | V | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | R | . | . | . | . | . | . | . | . | . | . | |||
Shangdong/02/2012 | . | . | . | . | . | . | . | . | . | . | . | V | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | R | . | . | . | . | . | . | R | . | . | . | |||
YZCQ/Liaoning/08 | . | . | . | . | . | . | . | . | . | . | . | V | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | . | . | . | . | . | . | . | . | D | . | . | |||
Beijing/01/2012 | . | . | . | . | . | . | . | . | . | . | . | V | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | R | . | . | . | . | . | . | R | . | . | . | |||
SD-02-11-Ch | . | . | . | . | . | . | . | . | . | . | . | V | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | R | . | . | . | . | . | . | R | . | . | . | |||
Ch/SD672/12 | . | . | . | . | . | . | . | . | . | . | . | V | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | R | . | . | . | . | . | . | . | . | . | ||||
Ch/SD754/12 | . | . | . | . | . | . | . | . | . | . | . | V | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | R | . | . | . | . | . | . | R | . | . | . | |||
Ch/SD755/12 | . | . | . | . | . | . | . | . | . | . | . | V | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | R | . | . | . | . | . | . | R | . | . | . | |||
Ch/SD758/12 | . | . | . | . | . | . | . | . | . | . | . | V | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | R | . | . | . | . | . | . | R | . | . | . | |||
Ch/SD01/13 | . | . | . | . | . | . | . | . | . | . | . | V | . | . | L | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | R | . | . | . | . | . | . | R | . | . | . | |||
TW/99-156B | . | . | . | . | . | . | . | . | . | . | . | V | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | ||||
TW-02-301 | . | . | . | . | . | . | . | . | . | . | S | V | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | . | . | . | . | . | . | . | . | . | . | ||||
TW-02-302 | . | . | . | . | . | . | . | . | . | . | S | V | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | . | . | . | . | . | . | . | . | . | . | ||||
TW-04-CB8 | . | . | . | . | . | . | . | . | . | . | S | V | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | . | . | . | G | . | . | . | . | . | . | ||||
TW/08-02 | . | . | . | . | . | . | . | . | . | . | S | V | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | M | . | . | . | . | . | G | . | . | . | . | . | ||||
strains | Amino acid residues | |||||||||||||||||||||||||||||||||||||||||||
101 | 112 | 117 | 121 | |||||||||||||||||||||||||||||||||||||||||
Clone 30 | L | D | A | Y | N | R | T | L | T | T | L | L | T | P | L | G | D | S | I | R | R | I | Q | E | S | V | T | T | S | G | G | G | R | Q | G | R | L | I | G | A | I | I | G | 123 |
B1 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
LaSota | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
Mukteswar | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | R | . | . | R | . | F | . | . | . | . | . | . | |
Herts/33 | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | R | . | . | R | . | F | . | . | . | . | . | . | |
NL/152608/93 | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | K | . | . | G | . | . | S | . | P | . | . | R | . | . | K | . | F | . | . | . | V | . | . | |
chicken/Kudus/018/10 | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | K | . | . | G | . | . | A | . | . | . | . | R | . | . | K | . | F | . | . | . | V | . | . | |
chicken/Sweden/97 | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | G | . | . | S | . | . | . | . | R | . | . | R | . | F | . | . | . | V | . | . | |
Sterna/Astr/2755/2001 | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | G | . | . | S | . | . | . | . | R | . | . | R | . | F | . | . | . | V | . | . | |
TW-99-175 | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | K | . | . | G | . | . | S | . | . | . | . | R | . | . | K | . | F | . | . | . | V | . | . | |
XJ-2/97 | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | K | . | . | G | . | . | S | . | . | . | . | R | . | . | K | . | F | . | . | . | V | . | . | |
JS/1/97/Go | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | A | . | . | . | . | . | R | . | . | R | . | F | . | . | . | . | . | . | |
BP01 | . | E | . | . | . | . | . | . | . | A | . | . | . | . | . | . | . | . | . | . | K | . | . | G | . | . | S | . | . | . | . | R | . | . | K | . | F | . | . | . | V | . | . | |
JS/5/06/Go | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | K | . | . | G | . | . | S | . | . | . | . | R | . | . | K | . | F | . | . | . | V | . | . | |
HA-14-07-Ch | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | K | . | . | G | . | . | S | . | . | . | . | R | . | . | K | . | F | . | . | . | V | . | . | |
JSD0812 | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | K | . | . | G | . | . | S | . | . | . | . | R | . | . | K | . | F | . | . | . | V | . | . | |
sh09 | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | K | . | . | G | . | . | Y | . | . | . | . | R | . | . | K | . | F | . | . | . | V | . | . | |
chicken/China/JSX1/2010 | . | E | . | . | . | . | . | . | . | A | . | . | . | . | . | . | . | . | . | . | K | . | . | G | . | . | S | . | . | . | . | R | . | . | K | . | F | . | . | . | V | . | . | |
JS-12-11-Ch | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | K | . | . | G | . | . | S | . | . | . | . | R | . | . | K | . | F | . | . | . | V | . | . | |
NDV/Chicken/TC/1/2011 | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | K | . | . | G | . | . | S | . | . | . | . | R | . | . | K | . | F | . | . | . | V | . | . | |
NDV/Chicken/TC/2/2011 | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | K | . | . | G | . | . | S | . | . | . | . | R | . | . | K | . | F | . | . | . | V | . | . | |
NDV/Chicken/TC/3/2011 | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | K | . | . | G | . | . | S | . | . | . | . | R | . | . | K | . | F | . | . | . | V | . | . | |
NDV/Chicken/TC/4/2011 | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | K | . | . | G | . | . | S | . | . | . | . | R | . | . | K | . | F | . | . | . | V | . | . | |
NDV/Chicken/TC/5/2011 | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | K | . | . | G | . | . | S | . | . | . | . | R | . | . | K | . | F | . | . | . | V | . | . | |
NDV/Chicken/TC/6/2011 | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | K | . | . | G | . | . | S | . | . | . | . | R | . | . | K | . | F | . | . | . | V | . | . | |
NDV/Chicken/TC/7/2011 | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | K | . | . | G | . | . | S | . | . | . | . | R | . | . | K | . | F | . | . | . | V | . | . | |
KNDV/Chicken/TC/8/2011 | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | K | . | . | G | . | . | S | . | . | . | . | R | . | . | K | . | F | . | . | . | V | . | . | |
NDV/Chicken/TC/9/2011 | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | K | . | . | G | . | . | S | . | . | . | . | R | . | . | K | . | F | . | . | . | V | . | . | |
PX2/03 | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | K | . | . | G | . | . | S | . | . | G | . | R | . | . | K | . | F | . | . | . | V | . | . | |
Hebei/02/2012 | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | K | . | . | G | . | . | S | . | . | . | . | R | . | . | K | . | F | . | . | . | V | . | . | |
Shangdong/02/2012 | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | K | . | . | G | . | . | S | . | . | . | . | R | . | . | K | . | F | . | . | . | V | . | . | |
YZCQ/Liaoning/08 | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | E | . | . | . | K | . | . | G | . | . | S | . | . | . | . | R | . | . | K | . | F | . | . | . | V | . | . | |
Beijing/01/2012 | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | K | . | . | G | . | . | S | . | . | . | . | R | . | . | K | . | F | . | . | . | V | . | . | |
SD-02-11-Ch | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | K | . | . | G | . | . | S | . | . | . | . | R | . | . | K | . | F | . | . | . | V | . | . | |
Ch/SD672/12 | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | K | . | . | G | . | . | S | . | . | . | . | R | . | . | K | . | F | . | . | . | V | . | . | |
Ch/SD754/12 | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | K | . | . | G | . | . | S | . | . | . | . | R | . | . | K | . | F | . | . | V | . | . | ||
Ch/SD755/12 | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | K | . | . | G | . | . | S | . | . | . | . | R | . | . | K | . | F | . | . | . | V | . | . | |
Ch/SD758/12 | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | K | . | . | G | . | . | S | . | . | . | . | R | . | . | K | . | F | . | . | . | V | . | . | |
Ch/SD01/13 | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | K | . | . | G | . | . | S | . | . | . | . | R | . | . | K | . | F | . | . | . | V | . | . | |
TW/99-156B | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | K | . | . | G | . | . | S | . | . | . | . | R | . | . | K | . | F | . | . | . | V | . | . | |
TW-02-301 | . | E | . | . | . | . | . | . | . | A | . | . | . | . | . | . | . | . | . | . | K | . | . | G | . | . | S | . | . | . | . | R | . | . | K | . | F | . | . | . | V | . | . | |
TW-02-302 | . | E | . | . | . | . | . | . | . | A | . | . | . | . | . | . | . | . | . | . | K | . | . | G | . | . | S | . | . | . | . | R | . | . | K | . | F | . | . | . | V | . | . | |
TW-04-CB8 | . | E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | K | . | . | G | . | . | S | . | . | . | . | R | . | K | K | . | F | . | . | . | V | . | . | |
TW/08-02 | . | E | . | . | . | . | . | . | . | A | . | . | . | . | . | . | . | . | . | . | K | . | . | G | . | . | S | . | . | . | . | R | . | . | K | . | F | . | . | . | V | . | . |
Sequence analysis of NDV isolates of HN gene of NDV
Amino acid residue substitutions at neutralizing epitopes on the HN glycoprotein among Shannxi isolates of NDV
Amino acid residues | ||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Strains | HR-A 74-89 | HR-B 96-110 | ||||||||||||||||||||||||||||||
Clone 30 | L | G | S | N | Q | D | V | V | D | R | I | Y | K | Q | V | A | L | L | N | T | E | T | T | I | M | N | A | I | S | L | ||
B1 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
LaSota | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | K | . | . | . | . | . | . | . | . | . | . | . | . | |
Mukteswar | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | S | I | . | . | . | . | . | . | . | . | |
Herts/33 | . | S | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | S | V | . | . | . | . | . | . | . | . | |
NL/152608/93 | . | S | . | S | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | . | . | S | I | . | . | . | . | . | . | . | . | |
chicken/Kudus/018/10 | . | S | . | S | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | . | . | S | M | . | . | . | . | . | . | . | . | |
chicken/Sweden/97 | . | S | . | S | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | . | . | S | I | . | . | . | . | . | . | . | . | |
Sterna/Astr/2755/2001 | . | . | . | S | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | . | . | S | I | . | . | . | . | . | . | . | . | |
TW-99-175 | . | S | . | S | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | . | . | S | I | . | . | . | . | . | . | . | . | |
XJ-2/97 | . | S | . | S | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | . | . | S | I | . | . | . | . | . | . | . | . | |
JS/1/97/Go | . | S | . | S | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | . | . | S | I | . | . | . | . | . | . | . | . | |
BP01 | . | S | . | S | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | . | . | S | I | . | . | . | . | . | . | . | . | |
JS/5/06/Go | . | S | . | S | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | . | . | S | . | . | . | . | . | . | . | . | . | |
HA-14-07-Ch | . | S | . | S | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | . | . | S | . | . | . | . | . | . | . | . | . | |
JSD0812 | . | S | . | S | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | . | . | S | I | . | . | . | . | . | . | . | . | |
sh09 | . | S | . | S | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | . | . | S | I | . | . | . | . | . | . | . | . | |
chicken/China/JSX1/2010 | . | S | . | S | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | . | . | S | . | . | . | . | . | . | . | . | . | |
JS-12-11-Ch | . | S | . | S | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | . | . | S | I | . | . | . | . | . | . | . | . | |
NDV/Chicken/TC/1/2011 | . | S | . | S | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | . | . | S | . | . | . | . | . | . | . | . | . | |
NDV/Chicken/TC/2/2011 | . | S | . | S | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | . | . | S | . | . | . | . | . | . | . | . | . | |
NDV/Chicken/TC/3/2011 | . | S | . | S | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | . | . | S | . | . | . | . | . | . | . | . | . | |
NDV/Chicken/TC/4/2011 | . | S | . | S | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | . | . | S | . | . | . | . | . | . | . | . | . | |
NDV/Chicken/TC/5/2011 | . | S | . | S | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | . | . | S | . | . | . | . | . | . | . | . | . | |
NDV/Chicken/TC/6/2011 | . | S | . | S | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | . | . | S | . | . | . | . | . | . | . | . | . | |
NDV/Chicken/TC/7/2011 | . | S | . | S | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | . | . | S | . | . | . | . | . | . | . | . | . | |
NDV/Chicken/TC/8/2011 | . | S | . | S | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | . | . | S | . | . | . | . | . | . | . | . | . | |
NDV/Chicken/TC/9/2011 | . | S | . | S | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | . | . | S | . | . | . | . | . | . | . | . | . | |
PX2/03 | . | S | . | S | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | . | . | S | I | . | . | . | . | . | . | . | . | |
Chicken/China/Hebei/02/2012 | . | S | . | S | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | . | . | S | I | . | . | . | . | . | . | . | . | |
Shangdong/02/2012 | . | S | . | G | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | . | . | S | V | . | . | . | . | . | . | . | . | |
YZCQ/Liaoning/08 | . | S | . | S | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | . | . | S | M | . | . | . | . | . | . | . | . | |
Beijing/01/2012 | . | S | . | S | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | . | . | S | V | . | . | . | . | . | . | . | . | |
SD-02-11-Ch | . | S | . | G | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | . | . | S | V | . | . | . | . | . | . | . | . | |
Ch/SD672/12 | . | S | . | G | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | . | . | S | V | . | . | . | . | . | . | . | . | |
Ch/SD754/12 | . | S | . | G | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | . | . | S | V | . | . | . | . | . | . | . | . | |
Ch/SD755/12 | . | S | . | G | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | . | . | S | V | . | . | . | . | . | . | . | . | |
Ch/SD758/12 | . | S | . | S | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | . | . | S | V | . | . | . | . | . | . | . | . | |
Ch/SD01/13 | . | S | . | G | . | . | . | I | . | . | . | . | . | . | . | . | . | . | . | . | . | S | V | . | . | . | . | . | . | . | . | |
TW/99-156B | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | |
TW-02-301 | . | S | . | S | . | . | . | M | . | . | . | . | . | . | . | . | . | . | . | . | . | S | I | . | . | . | . | . | . | . | . | |
TW-02-302 | . | S | . | S | . | . | . | M | . | . | . | . | . | . | . | . | . | . | . | . | . | S | I | . | . | . | . | . | . | . | . | |
TW-04-CB8 | . | S | . | S | . | . | . | M | . | . | . | . | . | . | . | . | . | . | . | . | . | S | I | . | . | . | . | . | . | . | . | |
TW/08-02 | . | S | . | S | . | . | . | M | . | . | . | . | . | . | . | . | . | . | . | . | . | S | I | . | . | . | . | . | . | . | . | |
Amino acid residues | ||||||||||||||||||||||||||||||||
Strains | Site 23 | Site 1 and 14 | Site 12 | Site 2 and 12 | Site 2 | |||||||||||||||||||||||||||
Clone 30 | 193-201 | 345-355 | 494 | 513-521 | 569 | |||||||||||||||||||||||||||
L | S | G | C | R | D | H | S | H | P | D | E | Q | D | Y | Q | I | R | M | A | G | R | I | T | R | V | S | S | S | D | |||
B1 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
LaSota | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
Mukteswar | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | D | . | . | . | . | . | . | . | . | . | G | |
Herts/33 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | D | . | . | . | . | . | . | . | R | . | . | |
NL/152608/93 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | D | . | V | . | . | . | . | . | . | . | . | |
chicken/Kudus/018/10 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | D | . | V | . | . | . | . | . | . | . | N | |
chicken/Sweden/97 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | D | . | V | . | Q | . | . | . | . | . | A | |
Sterna/Astr/2755/2001 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | D | . | V | . | . | . | . | . | . | . | V | |
TW-99-175 | . | . | . | . | . | . | . | . | . | . | . | G | . | . | . | . | . | . | . | . | D | . | V | . | . | . | . | . | . | . | . | |
XJ-2/97 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | D | . | V | . | . | . | . | . | . | . | . | |
JS/1/97/Go | . | . | . | . | . | . | . | . | . | . | . | G | . | . | . | . | . | . | . | . | D | . | V | . | . | . | . | . | . | . | . | |
BP01 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | D | . | V | . | . | . | . | . | . | . | . | |
JS/5/06/Go | . | . | . | . | . | . | . | . | . | . | . | G | . | . | . | . | . | . | . | . | D | . | V | . | . | . | . | . | . | . | . | |
HA-14-07-Ch | . | . | D | . | . | . | . | . | . | . | . | G | . | . | . | . | . | . | . | . | D | . | V | . | . | . | . | . | . | . | . | |
JSD0812 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | D | . | V | . | . | . | . | . | . | . | . | |
sh09 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | D | . | V | . | . | . | . | . | . | . | . | |
chicken/China/JSX1/2010 | . | . | . | . | . | . | . | . | . | . | . | G | . | . | H | . | . | . | . | . | D | . | V | . | . | . | . | . | . | . | . | |
JS-12-11-Ch | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | D | . | V | . | . | . | . | . | . | . | . | |
NDV/Chicken/TC/1/2011 | . | . | . | . | . | . | . | . | . | . | . | R | . | . | H | . | . | . | . | . | D | . | V | . | . | . | N | L | . | . | . | |
NDV/Chicken/TC/2/2011 | . | . | . | . | . | . | . | . | . | . | . | R | . | . | H | . | . | . | . | . | D | . | V | . | . | . | N | L | . | . | . | |
NDV/Chicken/TC/3/2011 | . | . | . | . | . | . | . | . | . | . | . | R | . | . | H | . | . | . | . | . | D | . | V | . | . | . | N | L | . | . | . | |
NDV/Chicken/TC/4/2011 | . | . | . | . | . | . | . | . | . | . | . | R | . | . | H | . | . | . | . | . | D | . | V | . | . | . | N | L | . | . | . | |
NDV/Chicken/TC/5/2011 | . | . | . | . | . | . | . | . | . | . | . | R | . | . | H | . | . | . | . | . | D | . | V | . | . | . | N | L | . | . | . | |
NDV/Chicken/TC/6/2011 | . | . | . | . | . | . | . | . | . | . | . | R | . | . | H | . | . | . | . | . | D | . | V | . | . | . | N | L | . | . | . | |
NDV/Chicken/TC/7/2011 | . | . | . | . | . | . | . | . | . | . | . | R | . | . | H | . | . | . | . | . | D | . | V | . | . | . | N | L | . | . | . | |
NDV/Chicken/TC/8/2011 | . | . | . | . | . | . | . | . | . | . | . | R | . | . | H | . | . | . | . | . | D | . | V | . | . | . | N | L | . | . | . | |
NDV/Chicken/TC/9/2011 | . | . | . | . | . | . | . | . | . | . | . | R | . | . | H | . | . | . | . | . | D | . | V | . | . | . | N | L | . | . | . | |
PX2/03 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | D | . | V | . | . | . | . | . | . | . | . | |
Chicken/China/Hebei/02/2012 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | D | . | V | . | . | . | . | . | . | . | . | |
Shangdong/02/2012 | . | . | . | . | . | . | . | . | . | . | . | K | . | . | . | . | . | . | . | . | D | . | V | . | . | . | . | . | . | . | . | |
YZCQ/Liaoning/08 | . | . | . | . | . | . | . | . | . | . | . | K | . | . | . | . | . | . | . | . | D | . | V | . | . | . | . | . | . | . | . | |
Beijing/01/2012 | . | . | . | . | . | . | . | . | . | . | . | K | . | . | . | . | . | . | . | . | D | . | V | . | . | . | . | . | . | . | . | |
SD-02-11-Ch | . | . | . | . | G | . | . | . | . | . | . | K | . | . | . | . | . | . | . | . | D | . | V | . | . | . | . | . | . | . | . | |
Ch/SD672/12 | . | . | . | . | . | . | . | . | . | . | . | K | . | . | . | . | . | . | . | . | D | . | V | . | . | . | . | . | . | . | . | |
Ch/SD754/12 | . | . | . | . | . | . | . | . | . | . | . | K | . | . | . | . | . | . | . | . | D | . | V | . | . | . | . | . | . | . | . | |
Ch/SD755/12 | . | . | . | . | . | . | . | . | . | . | . | K | . | . | . | . | . | . | . | . | D | . | V | . | . | . | . | . | . | . | . | |
Ch/SD758/12 | . | . | . | . | . | . | . | . | . | . | . | K | . | . | . | . | . | . | . | . | D | . | V | . | . | . | . | . | . | . | . | |
Ch/SD01/13 | . | . | . | . | . | . | . | . | . | . | . | K | . | . | . | . | . | . | . | . | D | . | V | . | . | . | . | . | . | . | . | |
TW/99-156B | - | - | - | - | - | - | - | - | - | . | . | G | H | . | . | . | . | . | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
TW-02-301 | . | . | . | . | K | . | . | L | . | . | . | G | . | . | . | . | T | . | . | . | D | . | V | . | . | . | . | . | . | . | . | |
TW-02-302 | . | . | . | . | K | . | . | . | . | . | . | G | . | . | . | . | T | . | . | . | D | . | V | . | . | . | . | . | . | . | . | |
TW-04-CB8 | . | . | . | . | K | . | . | . | . | . | . | G | . | . | . | . | T | . | . | . | D | . | V | . | . | . | . | . | . | . | . | |
TW/08-02 | . | . | . | . | . | . | . | . | . | . | . | G | . | . | . | . | T | . | . | . | D | . | V | . | . | . | . | . | . | . | . |
Phylogenetic analysis of F and HN genes of NDV
Relatedness (R) values of Shaanxi and LaSota strains
Hemagglutination inhibition assay
Number of chicken | Mean value | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Group | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
Oil- TC/1 | 6a
| 8 | 7 | 6 | 8 | 8 | 8 | 8 | 7 | 6 | 6 | 7 | 7.1 |
Oil-Las | 7 | 7 | 6 | 9 | 8 | 7 | 7 | 7 | 6 | 7 | 9 | 6 | 7.2 |
Live-Las | 7 | 7 | 7 | 8 | 7 | 8 | 6 | 7 | 6 | 6 | 7 | 8 | 7.0 |
Oil-TC/1 plus Live-Las | 8 | 8 | 8 | 9 | 8 | 10 | 8 | 7 | 8 | 6 | 8 | 9 | 8.1 |
Oil-Las plus Live-Las | 8 | 8 | 9 | 10 | 8 | 10 | 8 | 9 | 8 | 8 | 9 | 8 | 8.7 |
Un-inoculated control | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Negative control | 0 | 0 | 0 | 0 | 0 | 0 | - | - | - | - | - | - | 0 |
Neutralization test and cross-protectivity experiments
Serum of chicken | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Vaccine groupa
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
Oil-TC/1 | 128 | 256 | 256 | 64 | 256 | 256 | 256 | 256 | 128 | 64 | 128 | 128 |
Oil-Las | 64 | 64 | 32b
| 64 | 64 | 64 | 64 | 64 | 64 | 64 | 128 | 16b
|
Live-Las | 64 | 64 | 64 | 64 | 64 | 128 | 32b
| 64 | 16b
| 32b
| 64 | 64 |
Oil-TC/1 plus Live-Las | 64 | 64 | 64 | 128 | 256 | 128 | 128 | 128 | 128 | 256 | 256 | 256 |
Oil-Las plus Live-Las | 64 | 64 | 128 | 256 | 128 | 128 | 128 | 128 | 32b
| 64 | 128 | 128 |
NDV vaccine a
| Mortality in each challengeb (%) 7DPC c
| NDV-HI GMTd against LaSota strain 56 DPVe
| 14 DPC |
---|---|---|---|
Oil-TC/1 | 0 | 147 | 1024 |
Oil-Las | 17 | 147 | 1024 |
Live-Las | 25 | 156 | 2048 |
Oil-TC/1 plus Live-Las | 0 | 274 | 2048 |
Oil-Las plus Live-Las | 9 | 362 | 2194 |
Un-inoculated control | 100 | All died | All died |
Frequency of isolation of challenge virus in different vaccine groups
Group | Post-challenge samples (no. positive/total) | |||||||
---|---|---|---|---|---|---|---|---|
Day 2 | Day 4 | Day6 | Day9 | |||||
Oa
| Cb
| O | C | O | C | O | C | |
Oil- TC/1 | 12/12c
| 10/12 | 4/12 | 4/12 | 2/12 | 1/12 | 0 | 0 |
Oil-Las | 12/12 | 11/12 | 10/12 | 8/12 | 7/10 | 6/10 | 3/10 | 2/10 |
Live-Las | 12/12 | 10/12 | 9/12 | 8/12 | 5/10 | 4/10 | 2/9 | 2/9 |
Oil- TC/1plus Live-Las | 12/12 | 10/12 | 3/12* | 3/12* | 1/12 | 0 | 0 | 0 |
Oil-Las plus Live-Las | 12/12 | 11/12 | 8/12 | 7/12 | 5/12 | 3/12 | 2/11 | 1/11 |
Un-inoculated control | 12/12 | 12/12 | 12/12 | 12/12 | NSd
| NS | NS | NS |
Discussion
Conclusions
Materials and methods
Virus isolation and animals
Viral RNA extraction for reverse transcription (RT)-polymerase chain reaction (PCR)
Amino acid sequence and phylogenetic analyses
Hemagglutination inhibition assay
Cross virus-neutralization (VN) assay
Cross-protectivity
Group | Number of chicks | Vaccine dose | Challenged virus strain and dose |
---|---|---|---|
Oil-TC/1 | 12 | 0.4 ml | 106EID50 NDV/TC/1a
|
Oil-Las | 12 | 0.4 ml | 106EID50 NDV/TC/1 |
Live-Las | 12 | 1 dose | 106EID50 NDV/TC/1 |
Oil-TC/1 plus Live-Las | 12 | 0.4 ml/1 dose | 106EID50 NDV/TC/1 |
Oil-Las plus Live-Las | 12 | 0.4 ml/1 dose | 106EID50 NDV/TC/1 |
Un-inoculated control | 12 | PBS | 106EID50 NDV/TC/1 |
Negative control | 6 | PBS | PBS |