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01.12.2015 | Research article | Ausgabe 1/2015 Open Access

BMC Musculoskeletal Disorders 1/2015

Characterization of multinucleated giant cells in synovium and subchondral bone in knee osteoarthritis and rheumatoid arthritis

Zeitschrift:
BMC Musculoskeletal Disorders > Ausgabe 1/2015
Autoren:
Iván Prieto-Potin, Raquel Largo, Jorge A Roman-Blas, Gabriel Herrero-Beaumont, David A Walsh
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​s12891-015-0664-5) contains supplementary material, which is available to authorized users.

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

IP-P conceived of the study, participated in its design, acquisition of data, performed statistical analysis and interpretation of data, and drafted the manuscript. RL and JAR-B participated in the interpretation of data and helped to draft the manuscript. GH-B conceived of the study and design, and helped to draft the manuscript. DAW conceived of the study, design and coordination, interpretation of data and helped to draft the manuscript. All authors read and approved the final manuscript.

Abstract

Background

Multinucleated giant cells have been noticed in diverse arthritic conditions since their first description in rheumatoid synovium. However, their role in the pathogenesis of osteoarthritis (OA) or rheumatoid arthritis (RA) still remains broadly unknown. We aimed to study the presence and characteristics of multinucleated giant cells (MGC) both in synovium and in subchondral bone tissues of patients with OA or RA.

Methods

Knee synovial and subchondral bone samples were from age-matched patients undergoing total joint replacement for OA or RA, or non-arthritic post mortem (PM) controls. OA synovium was stratified by histological inflammation grade using index tissue sections. Synovitis was assessed by Krenn score. Histological studies employed specific antibodies against macrophage markers or cathepsin K, or TRAP enzymatic assay.

Results

Inflamed OA and RA synovia displayed more multinucleated giant cells than did non-inflamed OA and PM synovia. There was a significant association between MGC numbers and synovitis severity. A TRAP negative/cathepsin K negative Langhans-like subtype was predominant in OA, whereas both Langhans-like and TRAP-positive/cathepsin K-negative foreign-body-like subtypes were most commonly detected in RA. Plasma-like and foam-like subtypes also were observed in OA and RA synovia, and the latter was found surrounding adipocytes. TRAP positive/cathepsin K positive osteoclasts were only identified adjacent to subchondral bone surfaces. TRAP positive osteoclasts were significantly increased in subchondral bone in OA and RA compared to PM controls.

Conclusions

Multinucleated giant cells are associated with synovitis severity, and subchondral osteoclast numbers are increased in OA, as well as in RA. Further research targeting multinucleated giant cells is warranted to elucidate their contributions to the symptoms and joint damage associated with arthritis.
Zusatzmaterial
Additional file 1: Morphological MGC foam subtype and CD68 positive cells surrounding fat cells in OA and in RA. A and B. MGCs displaying a foam-like subtype were identified near to and surrounding fat cells in inflamed synovia from patients with either OA (A) or RA (B). Haematoxylin and eosin staining. Scale bar = 20 μm. Open arrows indicate foam-like MGC and A = adipocyte. C and D. Multiple mononuclear CD68 positive cells were found in a crown-like structure encircling adipocyte cells in both OA (C) and RA (D). Immunohistochemistry for CD68, using eosin contrast. Scale bar = 100 μm. (TIFF 3238 kb)
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Literatur
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