The non-tuberculous mycobacteria include those mycobacterium species that are not members of the Mycobacterium tuberculosis complex, the causative agent of pulmonary tuberculosis and Mycobacterium leprae. In Zambia, Non-tuberculous Mycobacteria are gaining recognition as pathogens of public health significance. However, there is scanty information on the isolation and speciation of these organisms for better patient management, consequently reducing the burden of these infections. Given the above information, the thrust of this study was to isolate and characterize NTM from humans and water in Namwala district of Zambia.
This was a cross-sectional study were 153 individuals with suspected TB were sampled from four health facilities in Namwala district, sputum samples were also collected. Additionally, 149 water samples were collected from different water drinking sources such as Tap water, Borehole water, rivers, wells and streams. Standard TB culture methods were employed to isolate Non-tuberculous Mycobacteria and later 16S–23S internal transcribed spacer region Sequencing was employed to characterize NTM.
Seven (7, 4.6%) NTM species were identified from humans with M. arupense (3, 42.9%) being the most common organism, while twenty three (23, 15.4%) NTM were identified from water with the common species being Mycobacterium gordonae (5, 21.7%). Mycobacterium avium and Mycobacterium fortuitum were both identified from human and water samples.
This study has shown the isolation of NTM species from humans and water. The isolation of NTM from drinking water sources could signify a public health risk to humans.
Akbar A, Derakhshaninezhad Z, Mirsaeidi M. Nontuberculous mycobacteria in Middle East : current situation and future challenges. Int J Mycobacteriol. 2015;12:1–11.
Asiimwe BB, Bagyenzi GB, Ssengooba W, Mumbowa F, Wajja A, Mayanja-kiiza H, Wbudeya E, Kallenius G, Joloba M. Species and genotypic diversity of non-tuberculous mycobacteria isolated from children investigated for pulmonary tuberculosis in rural Uganda. BMC Infect Dis. 2013;13:88. CrossRefPubMedPubMedCentral
Chanda-kapata P, Kapata N, Klinkenberg E, Mulenga L, Tembo M, Katemangwe P, Sunkutu V, Mwaba P, Grobusch M. Non-tuberculous mycobacteria ( NTM ) in Zambia: prevalence, clinical, radiological and microbiological characteristics. BMC Infect Dis. 2015;15:1264–6. CrossRef
Hongbao M, Young J, Shen C. RNA, DNA and protein isolation using TRI Zol reagent. Nat Sci. 2008;6(3):66–75.
Jarzembowski A, Young MB. Nontuberculous Mycobacterial infections. Arch Pathol Lab Med. 2008;132:1333–41. PubMed
LeDantec C, Duguet J, Mentiel A, Dumoutier D, Vincent V. Occurrence of Mycobacteria in water treatment lines and in water distribution systems. Appl Environ Microbiol. 2002;68:5318–25. CrossRef
Malama S, Muma J, Munyeme M, Mbulo G, Muwonge A, Shamputa IC, Djonne B, Godfroid J, Johansen TB. Isolation and Molecular Characterization of Mycobacterium tuberculosis from Humans and Cattle in Namwala District, Zambia. EcoHealth journal (International Association for Ecology and Health). 2014. doi: https://doi.org/10.1007/s10393-014-0940-0.
Munyeme M, Muma JB, Samui KL, Skjerve E, Nambota AM, Phiri IG, Rigouts L, Tryland M. Prevalence of bovine tuberculosis and animal level risk factors for indigenous cattle under different grazing strategies in the livestock/wildlife interface areas of Zambia. Tropl Anim Health Prod. 2009;41:345–52. CrossRef
Masaki T, Ohkusu K, Hata H, Fujiwara N, Lihara H, Yama-Noda M, Nhung PH, Hayashi A, Kawamura Y, Ezaki T. Mycobacterium kumamotonense sp. Nov. recovered from clinical specimen and the first isolation report of Mycobacterium arupense in Japan : novel slowly growing, Nonchromogenic clinical isolates related to Mycobacterium terrae Complex, Microbiolofy. Immunology. 2006;50:889–97.
Neonakis I, Gitti Z, Kontos F, Spandidos D. Mycobacterium aruprnse pulmonary infection: antibiotic resistance and restriction fragment length polymorphism analysis. Indian J Med Microbiol. 2008;28:173–6. CrossRef
Panagiotou M, Papaioannai AI, Kostitas K, Paraskeya M, Velentza E, Kanellopoulou M, Filaditaki. The Epidemiology of Pulmonary Nontuberculous Mycobacteria: Data from a General Hospital in Athens, Greece, 2007–2013, Pulmonary Medicine. 2014;2014:1–9.
Puthalapanttu S, Metersky M. Mycobacterium Nebraskense as a cause of nudular pulmonary disease. Conn Med. 2011;75:527–9.
Slany M, Svobodora J, Ettlova A, Slana I, Mrlik V, Pavlik I. Mycobacterium arupense among the isolates of non-tuberculous mycobacteria from human, animal and environmental samples. Vet Med. 2010;55:369–76.
Todorova TT, Kaludova V, Tsankova G, Ermenlieva N. A pulmonary infection caused by Mycobacterium peregrinum - a case report. J IMAB. 2015;21:1000–2. CrossRef
Tsai T, Lai C, Tsai I, Chang C, Hsiao P. Tenosynovitis caused by Mycobacterium arupense in a patient with diabetes mellitus. Clin Infect Dis. 2008;47:860–1. CrossRef
Velayati AA, Farnia P, Mozatari M, Mehdi M. Nontuberculous Mycobacteria isolation from clinical and environmental samples in Iran: twenty years of surveillance. Biomed Res Int. 2015;2015:1–10. CrossRef
- Characterization of non-tuberculous mycobacterium from humans and water in an Agropastoral area in Zambia
John Bwalya Muma
- BioMed Central
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