Background
Methods
Tissue specimens
Immunohistochemical staining procedure
Histopathological evaluation
Immunohistological evaluation
Statistical analysis
Results
Evaluation of atherosclerotic lesions
Aortic atherosclerosis | ||||||
---|---|---|---|---|---|---|
NO | I-II | III-IV | V-VI | |||
Total | 6 | 11 | 4 | 19 | ||
IHC score | n(%) | n(%) | n(%) | n(%) | r and p values | |
Periaortic fat chemerin expression
|
0
| 1(2.5) | 2(5) | 0(0) | 0(0) | r=0.824, p < 0.001a
|
1
| 5(12.5) | 5(12.5) | 0(0) | 0(0) | ||
2
| 0(0) | 4(10) | 1(2.5) | 4(10) | ||
3
| 0(0) | 0(0) | 3(7.5) | 15(37.5) | ||
Aortic VSMC chemerin expression
|
0
| 0(0) | 0(0) | 0(0) | 0(0) | r=0.626, p < 0.001a
|
1
| 3(7.5) | 4(10) | 1(2.5) | 0(0) | ||
2
| 3(7.5) | 7(17.5) | 1(2.5) | 9(22.5) | ||
3
| 0(0) | 0(0) | 2(5) | 10(25) | ||
Aortic foam cell chemerin expression
|
0
| -b
| 7(20.6) | 0(0) | 0(0) | r=0.670, p < 0.001a
|
1
| - | 0(0) | 0(0) | 0(0) | ||
2
| - | 1(2.9) | 0(0) | 1(2.9) | ||
3
| - | 3(8.8) | 4(11.8) | 18(52.9) | ||
Aortic VSMC CMKLR1 expression
|
0
| 6(15) | 8(20) | 3(7.5) | 13(32.5) | r=0.189, p=0.243 |
1
| 0(0) | 1(2.5) | 1(2.5) | 3(7.5) | ||
2
| 0(0) | 2(5) | 0(0) | 3(7.5) | ||
3
| 0(0) | 0(0) | 0(0) | 0(0) | ||
Aortic foam cell CMKLR1 expression
|
0
| -b
| 4(11.8) | 0(0) | 0(0) | r=0.424, p=0.012c
|
1
| - | 1(2.9) | 2(5.9) | 3(8.8) | ||
2
| - | 4(11.8) | 2(5.9) | 7(20.6) | ||
3
| - | 2(5.9) | 0(0) | 9(26.5) |
Coronary atherosclerosis | ||||||
---|---|---|---|---|---|---|
NO | I-II | III-IV | V-VI | |||
Total | 3 | 10 | 9 | 18 | ||
IHC score | n(%) | n(%) | n(%) | n(%) | r and p values | |
Pericoronary fat chemerin expression
|
0
| 0(0) | 0(0) | 0(0) | 0(0) | r=0.333, p=0.036a
|
1
| 1(2.5) | 4(10) | 2(5) | 0(0) | ||
2
| 1(2.5) | 3(7.5) | 3(7.5) | 8(20) | ||
3
| 1(2.5) | 3(7.5) | 4(10) | 10(25) | ||
Coronary VSMC chemerin expression
|
0
| 0(0) | 0(0) | 0(0) | 0(0) | r=0.059, p=0.717 |
1
| 1(2.5) | 1(2.5) | 0(0) | 1(2.5) | ||
2
| 1(2.5) | 7(17.5) | 7(17.5) | 13(32.5) | ||
3
| 1(2.5) | 2(5) | 2(5) | 4(10) | ||
Coronary foam cell chemerin expression
|
0
| -b
| 2(5.4) | 1(2.7) | 0(0) | r=0.405, p=0.013a
|
1
| - | 4(10.8) | 3(8.1) | 2(5.4) | ||
2
| - | 2(5.4) | 4(10.8) | 10(27) | ||
3
| - | 2(5.4) | 1(2.7) | 6(16.2) | ||
Coronary VSMC CMKLR1 expression
|
0
| 3(7.5) | 6(15) | 4(10) | 12(30) | r=0.005, p=0.977 |
1
| 0(0) | 0(0) | 3(7.5) | 1(2.5) | ||
2
| 0(0) | 2(5) | 2(5) | 4(10) | ||
3
| 0(0) | 2(5) | 0(0) | 1(2.5) | ||
Coronary foam cell CMKLR1 expression
|
0
| -b
| 6(16.2) | 3(8.1) | 4(10.8) | r=0.317, p=0.056 |
1
| - | 2(5.4) | 1(2.7) | 4(10.8) | ||
2
| - | 2(5.4) | 3(8.1) | 7(18.9) | ||
3
| - | 0(0) | 2(5.4) | 3(8.1) |
Chemerin and CMKLR1 expression in periaortic adipose tissue, aortic vascular smooth muscle cells and foam cells in aortic atherosclerotic lesions
Chemerin and CMKLR1 expression in pericoronary adipose tissue, coronary vascular smooth muscle cells and foam cells in coronary atherosclerotic lesions
Chemerin and CMKLR1 expression in association with atherosclerosis
Expression of chemerin and CMKLR1 in association with age and clinical parameters
Age group | |||||||
---|---|---|---|---|---|---|---|
0-20 | 21-40 | 41-60 | 61-80 | 80+ | |||
IHC score | n(%) | n(%) | n(%) | n(%) | n(%) | r and p values | |
Total | 5(12.5) | 13(32.5) | 12(30) | 9(22.5) | 1(2.5) | ||
Periaortic fat chemerin expression
|
0
| 2(5) | 1(2.5) | 0(0) | 0(0) | 0(0) | r=0.664, p < 0.001a
|
1
| 2(5) | 7(17.5) | 1(2.5) | 0(0) | 0(0) | ||
2
| 1(2.5) | 1(2.5) | 6(15) | 1(2.5) | 0(0) | ||
3
| 0(0) | 4(10) | 5(12.5) | 8(20) | 1(2.5) | ||
Total | 5(12.5) | 13(32.5) | 12(30) | 9(22.5) | 1(2.5) | ||
Aortic VSMC chemerin expression
|
0
| 0(0) | 0(0) | 0(0) | 0(0) | 0(0) | r=0.430, p=0.006a
|
1
| 1(2.5) | 6(15) | 1(2.5) | 0(0) | 0(0) | ||
2
| 4(10) | 5(12.5) | 5(12.5) | 6(15) | 0(0) | ||
3
| 0(0) | 2(5) | 6(15) | 3(7.5) | 1(2.5) | ||
Total | 3(8.8) | 9(26.4) | 12(35.3) | 9(26.4) | 1(2.9) | ||
Aortic foam cell chemerin expression
b
|
0
| 2(5.9) | 4(11.8) | 1(2.9) | 0(0) | 0(0) | r=0.554, p=0.001a
|
1
| 0(0) | 0(0) | 0(0) | 0(0) | 0(0) | ||
2
| 0(0) | 1(2.9) | 1(2.9) | 0(0) | 0(0) | ||
3
| 1(2.9) | 4(11.8) | 10(29.4) | 9(26.5) | 1(2.9) | ||
Total | 3(8.8) | 9(26.4) | 12(35.3) | 9(26.4) | 1(2.9) | ||
Aortic foam cell CMKLR1 expression
b
|
0
| 2(5.9) | 2(5.9) | 0(0) | 0(0) | 0(0) | r=0.433, p=0.01c
|
1
| 0(0) | 1(2.9) | 4(11.8) | 1(2.9) | 0(0) | ||
2
| 0(0) | 5(14.7) | 5(14.7) | 3(8.8) | 0(0) | ||
3
| 1(2.9) | 1(2.9) | 3(8.8) | 5(14.7) | 1(2.9) |
Total | No hypertension | Hypertension | ||
---|---|---|---|---|
20 | 20 | |||
IHC score | n(%) | n(%) | p value | |
Periaortic fat chemerin expression
|
0
| 3(7.5) | 0(0) | p=0.044a
|
1
| 7(17.5) | 3(7.5) | ||
2
| 3(7.5) | 6(15) | ||
3
| 7(17.5) | 11(27.5) | ||
Aortic VSMC chemerin expression
|
0
| 0(0) | 0(0) | p=0.021a
|
1
| 5(12.5) | 3(7.5) | ||
2
| 13(32.5) | 7(17.5) | ||
3
| 2(5) | 10(25) | ||
Other cause
|
Acute coronary occlusion
| |||
Total
|
30
|
10
| ||
IHC score
|
n(%)
|
n(%)
|
p value
| |
Total | 27(72.9) | 10(27) | ||
Coronary foam cell chemerin expression
b
|
0
| 3(8.1) | 0(0) | p=0.018a
|
1
| 8(21.6) | 1(2.7) | ||
2
| 12(32.4) | 4(10.8) | ||
3
| 4(10.8) | 5(13.5) |