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Erschienen in:

24.09.2019 | Original Article

Cholesterol 25-hydroxylase suppresses rabies virus infection by inhibiting viral entry

Erschienen in: Archives of Virology | Ausgabe 12/2019

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Abstract

Cholesterol-25-hydroxylase (CH25H) is a reticulum-associated membrane protein that catalyzes the oxidation of cholesterol to 25-hydroxycholesterol (25HC). Recent studies have revealed that CH25H is an interferon-stimulated gene (ISG) that suppresses infection by several viruses. In the present study, we found that overexpression of both human and murine CH25H inhibited rabies virus (RABV) infection in HEK-293T (293T) cells. In contrast, silencing of CH25H enhanced RABV replication in 293T cells, and a catalytic mutant of CH25H lost its ability to inhibit RABV infection. Treatment with the oxysterol 25-hydroxycholesterol (25HC), the product of CH25H, dramatically decreased RABV replication in 293T, BSR and N2a cells by inhibiting viral membrane penetration. These data provide insights into the antiviral function of CH25H against RABV infection, which can potentially be used as a therapeutic agent for rabies.

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Titel: Cholesterol 25-hydroxylase suppresses rabies virus infection by inhibiting viral entry
Publikationsdatum: 24.09.2019
Erschienen in: Archives of Virology / Ausgabe 12/2019
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-019-04415-6

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Cholesterol 25-hydroxylase suppresses rabies virus infection by inhibiting viral entry

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