The online version of this article (doi:10.1186/1477-7819-10-198) contains supplementary material, which is available to authorized users.
The authors declare that they have no competing interests.
RD and PK wrote the article. RT, SB, RM and VKS contributed to the concept, study design, data analysis, interpretation of results and approved the final manuscript.
The etiopathogenesis of gallbladder cancer is still unknown. Both environmental and patient factors have been incriminated in its cause. That it is found in pockets of epidemiological distribution raises an issue of genetic changes associated with it. The aim of this study was to find out the chromosomal changes in gallbladder cancer.
Lymphocyte cell culture was carried out on blood of gallbladder cancer patients to determine chromosomal banding abnormalities. Native PAGE was also evaluated to analyze lactate dehydrogenase (LDH), superoxide dismutase (SOD) and catalase enzyme activity from the same blood of gallbladder cancer patients.
Out of 30 gallbladder cancer patients, 4 male showed breakage on the long arm of chromosome 1 while only one male patient showed the translocation from the long arm of chromosome 4 to the long arm of chromosome 6 in a male patient.
The aberrations found in our study may suggest underlying genetic predisposition for the development of gallbladder cancer. They can act as a marker for gallbladder cancer, which needs further study.
Pathak S, Berry KK, Burke TW, Baker VV: Identification of primary chromosome abnormalities in a patient with endometrial carcinoma: analysis of tumor biopsy and lymphocyte cultures. Int J Oncol. 1995, 7: 765-772. PubMed
Mishra R, Shukla SP: Immunochemical detection of serum LDH1: an indigenous method in diagnosis of myocardial infraction. Curr Sci. 1999, 76: 587-590.
Schottenfeld D, Fraumeni JF: Cancer Epidemiology and Prevention. 2006, New York: Oxford University Press USA CrossRef
Pathak S, Dhaliwal MK: Cytogenetic lesions in renal cell carcinoma. Cancer Bull. 1989, 41: 324-329.
Pathak S, Hopwood VL, Hortobagyi GN, Jackson GL, Hughes JI, Melillo D: Chromosome anomalies in human breast cancer. Evidence for specific involvement of 1q region in lymphocyte cultures. Anticancer Res. 1991, 11: 1055-1060. PubMed
Ghayee HK, Dinney CPN, Pathak S: Do lymphocytes contain chromosomal lesions that are also stable markers in cancer cells? Lymphocyte and tumor cell karyotyping in a melanoma patient. Int J Oncol. 1997, 11: 681-684. PubMed
- Chromosomal structural analysis in carcinoma of the gallbladder
- BioMed Central
Neu im Fachgebiet Chirurgie
Mail Icon II