Background
Oral squamous cell carcinoma (OSCC) is defined as an invasive epithelial tumor with different degrees of differentiation; it is prone to lymph node metastasis and distant metastasis [
1]. Oral cancer accounts for about 3% of malignant tumors worldwide [
2]. A total of 1.6 million people was diagnosed with head and neck squamous cell carcinoma [
3,
4]. Recent research has shown that the 5-year survival rate of patients with OSCC is about 60%, and it is even lower for patients with advanced OSCC [
2,
5].
Circular RNA (circRNA) is an endogenous, stable, and conserved non-coding RNA molecule that forms a cyclic ring by covalent bonds. circRNA is abundant in the eukaryotic transcriptome. It has a cell type- or developmental stage-specific expression pattern in eukaryotic cells [
6,
7]. The specificity of the circRNA structure determines its specific biological functions, such as functions in sponge adsorption [
8], the regulation of gene transcription [
9], and protein translation [
10]. Relationships between circRNAs and diseases, especially tumors, have recently been reported [
11], including relationships with gastric cancer [
12], breast cancer [
13], and liver cancer [
14]. This provides an important molecular biological basis for understanding the complex development of tumors.
The occurrence and development of OSCC is regulated at the gene level, and gene expression is regulated at the DNA, transcriptional, post-transcriptional, and translation levels in a complex process [
15]. However, little is known about the expression and functions of circRNAs in OSCC. In this experimental study, the correlation between hsa_circ_0055538 and the malignant biological behavior of OSCC was explored, including the potential application of this circRNA in molecular diagnosis and treatment.
Materials and methods
Patients and tissue samples
According to WHO diagnostic criteria, 44 patients with OSCC were admitted to the Department of Oral and Maxillofacial Surgery, Peking University Shenzhen Hospital from 2016 to 2018. All cases were confirmed by histopathology. All patients underwent complete resection of the primary tumor after admission and radical or functional neck dissection according to the condition. Exclusion criteria were as follows: patients receiving radiotherapy, chemotherapy, or biotherapy before surgery and patients with systemic diseases, such as immune system diseases, hyperthyroidism, diabetes, and heart disease. All patients provided informed consent in accordance with the ethical guidelines of Peking University (Protocol No. 37923/2-3-2012). The study was approved by the Ethics Committee of Peking University Health Science Center (IRB00001053-08043).
Cell culture and transfection
The human OSCC cell lines SCC9, SCC15, SCC25, and CAL27 were obtained from the College of Stomatology, Wuhan University (Wuhan, China). Normal oral epithelial keratinocyte (HOK) cells were obtained from the cell bank of the Chinese Academy of Sciences (Shanghai, China). SCC15, SCC25, CAL27, and HOK cell lines were cultured in Dulbecco’s modified Eagle medium (DMEM; Gibco, Grand Island, NY, USA) containing 10% fetal bovine serum (FBS; Gibco). SCC9 cells were cultured in 1:1 DMEM/Ham’s F12 medium containing 10% FBS and 1% P/S. All cells were cultured at 37 °C and 5% CO2 in a humidified atmosphere. The experimental cells were all in the logarithmic growth phase. Transfected siRNA (5′-AAGTCTGCCAAGATGCTGAAT-3′) was synthesized by Guangzhou RiboBio Co. (Guangzhou, China). The cells were incubated with p53 activator tenovin-1 (p53 AT; Selleck, Shanghai, China) at a concentration of 12 μM for 8 h before subsequent experiments.
Lentivirus infection and cell screening
The target cells were inoculated into a 24-well plate at a density of 1 × 10 6 cells/mL, and the cells reached about 50% confluence after 1 day, when the virus infection was performed. Polybrene (10 μg/mL) was added to the medium to enhance the infection efficiency. After inoculating the target cells with a lentivirus vector for 48 h, fresh complete medium containing appropriate concentrations of puromycin (12 μg/mL for SCC9 cells; 10 μg/mL for CAL27 cells) was used to screen stable transfected cell lines. PCR was used to detect the expression of the gene of interest. The lentiviral vector used in the experiment was pHBLV-CMV-crRNA-EF1-GFP-T2A-puro (HanBio Co. Ltd., Shanghai, China) .
qRT-PCR analysis
Total RNA was isolated using an RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. RNase R treatment was performed for 15 min at 37 °C (Epicentre, Madison, WI, USA) at 3 U/mg. PCR was performed using 2 × PCR Master Mix (Thermo Fisher Scientific, Waltham, MA, USA). The ΔΔC
T method was used to calculate the relative expression levels of different genes, and glyceraldehyde 3-phosphate dehydrogenase (
GAPDH) was used as an internal control. Primers for qRT-PCR were as follows:
-
hsa_circ_0055538-F, 5′-AGGCCTGCGAAGGAAACTTA-3′;
-
hsa_circ_0055538-R, 5′-TTGTGGTCGGAGGCCAATTT-3′;
-
GAPDH-F, 5′-TCAAGGCTGAGAACGGGAAG-3′;
-
GAPDH-R, 5′-TCGCCCCACTTGATTTTGGA-3′;
-
p53-F, 5′-TGACACGCTTCCCTGGATTG-3′;
-
p53-R, 5′-TCCGGGGACAGCATCAAATCA-3′;
-
p21-F, 5′-CTCAGAGGAGGCGCCATGT-3′;
-
p21-R, 5′-GCCTCCTCCCAACTCATCCC-3′;
-
cleaved caspase-3-F, 5′-ACCAAAGGCTGTATGCGCTG-3′;
-
cleaved caspase-3-R, 5′-TCACCAGCTCAATTGCAAAGGG-3′;
-
bax-F, 5′-CCCAGAGGCGGGGTTTCA-3′;
-
bax-R, 5′-CAGCTTCTTGGTGGACGCAT-3′;
-
bcl-2-F, 5′-AGTACCTGAACCGGCACCTG-3′;
-
bcl-2-R, 5′-CACAAAGGCATCCCAGCCTC-3′;
-
apaf-1-F, 5′-TGGACACCTTCTTGGACGACA-3′;
-
apaf-1-R, 5′-CTCTGCAATCAGCCACCTTTGA-3′;
-
caspase-3-F, 5′-TTCATTATTCAGGCCTGCCG-3′;
-
caspase-3-R, 5′-GAGCCATCCTTTGAATTTCGC-3′.
-
RMND5A-F, 5′-ACAGCAGTGTTTCTCGGGTT-3′;
-
RMND5A-R, 5′-GTTTGACACTGCCCACTCCA-3′.
Cell Counting Kit-8 (CCK-8) assay
The stably infected cells were uniformly plated in a 96-well plate to ensure 2000 cells/well. Five sub-holes were established simultaneously. Then, 10 µL of CCK-8 liquid (RIBO Biotechnology Co. Ltd., Guangzhou, China) was added dropwise at 24 h, 48 h, 72 h, and 96 h, and incubation was continued for 1 h. Absorbance values at 450 nm and 630 nm were measured using a microplate reader.
5-Ethynyl-2′-deoxyuridine (EdU) incorporation assay
The stably infected cells of interest were seeded in 96-well plates at 4 × 104 cells per well and cultured to the logarithmic growth phase. The cells were treated with EdU solution, Apollo staining reaction solution, and Hoechst33342 reaction solution (Beyotime Biotechnology, Shanghai, China). Cells were observed under an inverted fluorescence microscope, and images were obtained.
Flow cytometry
The stably infected cells of interest were evenly plated into 6-well plates, and the cells were grown to log phase for experiments. Cells were harvested by trypsin digestion without EDTA. The cells were resuspended using annexin-binding buffer, and cells were incubated with annexin V working solution and PI working solution for 15 min (Beyotime Biotechnology). Apoptosis was analyzed by flow cytometry (BD Biosciences, Franklin Lakes, NJ, USA).
Wound healing assay
The stably infected target cells were evenly plated into a 6-well plate, and the cells were grown to 90% confluency on the next day. A 200-μL sterile tip perpendicular to the 6-well plate was used for scratches to ensure a consistent scratch width. Images were obtained at 0 h. The culture was continued in DMEM containing no FBS, and images were obtained after 24 h.
Migration and invasion assays
Migration and invasion assays were performed using Transwell and Matrigel pre-coated Transwell chambers, respectively (Corning Life Sciences, Corning, NY, USA). A target cell resuspension containing no FBS was added to the upper chamber, and DMEM with 10% FBS was added to the lower chamber. After 24 and 48 h of culture, cell fixation, staining, and imaging were performed.
Western blot analysis
The stably infected target cells were evenly plated on 6-well plates, and the cells were harvested when they reached 80% confluency. Total protein was extracted using RIPA lysis buffer, and the protein concentration was determined using BCA working solution (Beyotime Biotechnology). The loading protein buffer was prepared using Loading Buffer. Protein electrophoresis, membrane transfection, blocking, and incubation with primary and secondary antibodies were performed. Chemical color development was conducted using a luminescent liquid (Millipore Sigma, Burlington, MA, USA). A western blot analysis was performed using commercial primary antibodies against the following proteins: GAPDH (1:1000; #5174), p53 (1:1000; #48818), p21 (1:1000; #2947), cleaved caspase-3 (1:1000; #9661), Bax (1:1000; #14796), Bcl-2 (1:1000; #2872), Apaf-1 (1:1000; #8969), and caspase-3 (1:1000; #9662; all from Cell Signaling Technology, Danvers, MA, USA). The secondary antibodies were as follows: horseradish peroxidase-conjugated goat anti-rabbit (1:1000; A0208) and goat anti-mouse (1:1000; A0216; both from Beyotime Biotechnology).
Tumorigenesis and staining
Infected SCC9 cells (1 × 107 cells/100 μL) were injected into 16 4-week-old BALB/c athymic nude mice (Siliake Jingda Experimental Animal Co. Ltd., Hunan, China). Tumor volume, measured weekly, was calculated as V = πAB2/6, where V = tumor volume, A = largest diameter, and B = perpendicular diameter. After 6 weeks, mice were euthanized after the injection of excess anesthetic. Tumor tissue was collected and weighed. Part of the tumor tissue samples was used to extract total protein. The remaining tumor tissue specimens were placed in 10% neutral formalin buffer for 48 h, embedded in paraffin by a conventional embedding method, and sectioned. The paraffin sections were subjected to hematoxylin staining and dewaxing, sealed with a neutral gum, and observed under an inverted microscope.
Image processing and statistical analysis
All images were obtained by wide-field microscopy. Results are presented as mean ± standard error of the mean (SEM) from three independent experiments. All statistical data were analyzed using SPSS 17.0 (IBM, Chicago, IL, USA). Two-tailed Student’s t-tests were used to evaluate difference among groups; P < 0.05 was considered significant.
Discussion
Oral cancer is the sixth most common cancer in the world, and its incidence varies among ecogeographic regions [
16]. Despite recent improvements in treatments and medical management, approximately 540,000 new cases are diagnosed each year and survival rates have not improved significantly. Therefore, OSCC has gradually become a major public health issue. The specificity of the anatomy as well as the malignant biological behavior of OSCC pose serious challenges to its treatment. For early squamous cell carcinoma of the head and neck, good results can be obtained using surgery or radiotherapy [
17]. Unfortunately, 60% of head and neck squamous cell carcinomas are detected at stage III/IV, which means that most patients already have lymph node metastasis or distant metastases. Although surgery plus concurrent radiotherapy and chemotherapy can delay progression, most studies have shown that this strategy does not significantly improve the long-term survival rate [
18].
The formation of circRNAs can occur in any region of the genome; they can range in length from a few hundred to several thousand nucleotides [
19,
20]. CircRNAs have been considered ancient, conserved molecules resulting from abnormal splicing and have been described as “dark matter” in organisms [
21]. In recent years, with the development of high-throughput sequencing technology and improvements in data analysis techniques [
22], this “dark matter” has gradually been characterized and has become a major area of research in studies of non-coding RNA [
23].
Studies have shown that the expression levels of some circRNAs differ significantly between tumor tissues and normal tissues and are associated with clinical features, such as distant metastasis and TNM stage, providing new insight into the pathogenesis of tumors [
24]. Similar to other malignancies, OSCC develops by a complex series of cellular biological processes involving both coding and non-coding genes [
25]. Using a bioinformatics approach, Wang et al. [
26] demonstrated that circDOCK1 regulates BIRC3 expression by functioning as a competing endogenous RNA (ceRNA) and participates in OSCC apoptosis. Chen et al. [
27] showed that circRNA_100290 is up-regulated in OSCC tissues and is co-expressed with CDK6. circRNA_100290 can act as a competitive endogenous RNA and regulates the expression of CDK6 by sponge-adsorbing miR-29b family members, thereby affecting the malignant biological behavior of OSCC [
27]. We obtained microarray circRNA expression profiles from patients with OSCC (n = 8) and controls (n = 8) by high-throughput sequencing [
28] and found that hsa_circ_0055538 is significantly underexpressed in tumor tissues. We performed q-PCR detection and verification using 44 pairs of cancer and adjacent tissue specimens. Using clinical data, we found that the expression level of hsa_circ_0055538 is correlated with the degree of tumor differentiation. The lower the degree of tumor differentiation, the higher the malignancy of the tumor and the worse the long-term survival rate. These results support the potential clinical value of hsa_circ_0055538. We then performed a functional assay using OSCC cell lines. We found that when the OSCC cell lines SCC9 and CAL27 expressed high levels of hsa_circ_0055538, their proliferative capacity, migration ability, and invasion ability were significantly inhibited. Expression of the circRNA also promoted cell apoptosis. These results suggest that changes in the expression level of hsa_circ_0055538 affect the malignant biological behavior of OSCC cell lines. To further explore the mechanism of action of this circRNA in OSCC, we examined target proteins by western blotting.
The
p53 gene is a common tumor suppressor located on chromosome 17p [
29]. It is involved in cell cycle regulation via a variety of pathways and plays an important role in the development of various tumors, including OSCC [
30]. BAX is a water-soluble protein homologous to BCL-2 and promotes apoptosis. The overexpression of BAX can antagonize the protective effect of BCL-2 and cause cell death. It is located downstream of the p53 signaling pathway and is regulated by the
p53 gene [
31]. Apoptotic protease activating factor-1 (Apaf-1) plays an important role in the mitochondrial apoptotic pathway, and its expression is regulated by the
BAX gene [
32]. Apaf-1 ultimately mediates caspase family-related proteins, such as caspase-3, which is generally considered the most important terminal cleavage enzyme in apoptosis [
33]. Our experimental results showed that when hsa_circ_0055538 was overexpressed in SCC9 and CAL27 cells, the expression levels of p53, p21, BAX, Apaf-1, caspase-3, and cleaved caspase-3 increased, while the expression of Bcl-2 decreased. We knocked down hsa_circ_0055538 in SCC9 and CAL27 cells using siRNA and obtained the opposite results. The expression of these genes was also confirmed at the mRNA level. Furthermore, we overexpressed p53 after knocking down hsa_circ_0055538 and performed a CCK-8 assay, wound healing assay, and invasion assay, which showed that the proliferation, migration, and invasion of tumor cells in the experimental group were inhibited compared with those in the control group. These results suggest that the circRNA regulates the malignant biological behavior of OSCC via the p53 signaling pathway and may be involved in the regulation mechanism of the cell cycle. In addition, overexpressing p53 after knocking down hsa_circ_0055538 rescued the phenotype observed with a low level of hsa_circ_0055538. Our results also indicated that overexpression of hsa_circ_0055538 in SCC9 and CAL27 cells decreased the mRNA level of
RMND5A, and vice versa. This suggested that the change of hsa_circ_0055538 expression level may affect the transcription of its parent gene and play a potential role in negative feedback regulation.
To further verify the effect of hsa_circ_0055538 on the tumorigenic ability of OSCC, we performed a tumor-forming experiment using nude mice. The experimental results showed that the tumorigenic ability of tumor cells in vitro was significantly inhibited by the high expression of hsa_circ_0055538. We also detected higher p53 expression in tumor tissues of the experimental group than in the control group. These findings are consistent with the results of the cytology experiment. The above experimental results indicate that the circRNA may regulate the development of OSCC via the p53 signaling pathway. Studies of specific drugs targeting the p53 signaling pathway based on this circRNA are needed [
34].
Authors’ contributions
WS performed the experiments. HY and YS designed the experiments. SS and FW performed the qRT-PCR analysis. WS was a major contributor in writing the manuscript. All authors read and approved the final manuscript.