Background
Kawasaki disease(KD) is an acute febrile illness, known as mucocutaneous lymph node syndrome, which mainly occurs in boys under 5 years old [
1]. Cardiovascular manifestations are the main complications of KD such as coronary artery abnormalities, myocarditis, pericarditis, pericardial effusion, valvular dysfunction, left ventricular dysfunction, and arrhythmias [
2]. And KD is the most common cause of coronary artery aneurysms (CAA) in children or young adults and the leading cause of acquired heart disease [
3]. However, treatment of intravenous immunoglobulin (IVIG) plus acetylsalicylic acid (ASA) reduces the prevalence of coronary artery abnormalities from 32 to 50% to approximately 4% [
4]. Although the pathogenesis of KD is still not fully understood, innate and specific immunity are always fully activated during the acute stage of KD, in which neutrophils, CD8 + T cells, dendritic cells, and macrophages are also activated successively in infiltration of artery walls [
5,
6], then leading to the activation of the nuclear transcription factor NF-κB in monocytes macrophages [
7]. This promotes the production of inflammatory cytokines such as IL-6 and TNF-α [
8], further infiltrating vascular endothelium and causing immune activation.
Adipose tissue is not only a simple energy metabolism organ but also an important endocrine organ that could secrete numerous of proinflammatory cytokines such as TNF, IL-6, MCP1, leptin, and others [
9,
10]. Also, it can secrete a series of anti-inflammatory adipokines including the CTRP family and Sfrp5, which play crucial protective roles in the inflammation and atherosclerosis [
11,
12]. Adipokines are these pleiotropic molecules mainly secreted by adipocytes. It demonstrated that classical adipokines leptin, adiponectin and resistin play a major role in energy metabolism, inflammation, obesity, diabetes, cardiovascular disease and autoimmune diseases [
13‐
16]. Recently, it has been conducted that adipokines including adiponectin, leptin, resistin, and visfatin were involved in the acute stage of KD and may participate in its progress of coronary artery lesions [
17‐
19]. Therefore, it interests us that whether the more recently identified chemerin, and omentin, play roles in Kawasaki disease and whether they are associated with lipid metabolism disorders and coronary artery abnormalities of KD. In this study, we focus on the anti-inflammatory and pro-inflammatory effects of chemerin,omentin-1, and adiponectin on the acute phase of KD. Fortunately, it showed that adipokines including chemerin, omentin-1 and adiponectin involved in inflammation of acute KD and might be associated with its lipid metabolism disorders in this study.
Discussion
As recently discovered adipokines, chemerin, omentin-1, and adiponectin play crucial roles in inflammatory response and are closely involved in cardiovascular diseases. Takeshida et al. [
20] reported that adiponectin levels were significantly higher in KD patients; Nozue et al. [
21] also found resistin levels elevated in KD but its concentrations were unlikely to predict the prognosis of the disease in the acute stage; Liu et al. [
22] published data suggesting that leptin participates in the systemic inflammatory response but with controversial results; Kim et al. [
18] found that resistin is significantly higher in KD patients, although it has no prognostic value to predict coronary anomalies in the acute stage.
This study aimed to evaluate the levels of these three adipokines in the acute phase of KD and to investigate the associations between adipokines and coronary artery abnormalities or lipid metabolism disorders of KD. Our research demonstrated that (1) circulating chemerin levels are increased in the acute stage of KD. (2)By contrast, omentin-1 and adiponectin levels are decreased compared with general fever patients in the acute stage of KD. (3)Furthermore, cytokine IL-1β is elevated in patients with acute KD, which is similar to inflammatory cytokines such as TNF-α. On the other hand, IL-1β has a decreasing trend in CAL compared with the NCAL group, which might imply that IL-1β involved in the process of inflammatory infiltration and immune vasculitis in the acute phase of KD. Moreover, (4) we find that circulating omentin-1 is positively correlated with both chemerin and total cholesterol, which suggests that chemerin and omentin-1 are involved in the lipid metabolism disorders in the acute stage of KD.
Chemerin was identified as a cDNA sequence called TIG2 (Tazarotene-induced gene 2) in 1997 firstly [
23], whose expression was up-regulated during the treatment of psoriatic lesion by tazarotene, which is a kind of retinoic acid receptors (RARs). It has been demonstrated that the chemerin as a type of adipokine, whose gene expression and its receptor, chemerin-like receptor1 (CMKLR1), was significantly higher in adipose tissue of obese models through a signal sequence trap in 2007 in the first place [
24].Our study firstly indicated that circulating chemerin levels increased significantly in the acute stage of KD, which were associated with its effects on the early phase of immune response and inflammatory reaction. Chemerin could modulate immune responses through its chemotactic effects and accumulation of antigen-presenting cells including macrophages and dendritic cells at the sites of damage areas [
25,
26]. Several studies have reported that chemerin was closely associated with the inflammatory response related to obesity, metabolic syndrome, rheumatoid arthritis and cancer [
27‐
30]. Chemerin receptor CMKRL1 expressed in human vascular endothelial cells could bind to chemerin, which induces inflammation and angiogenesis processes [
31,
32]. In addition, chemerin was involved in the development of inflammation in cardiovascular disease and atherosclerosis [
33‐
35], and circulating chemerin were associated with soluble ICAM-1 and E-selectin [
36], which provide the greatest evidence regarding endothelial-cell activation that could trigger vascular inflammation. We didn’t find a direct relationship between circulating chemerin and KD with coronary artery abnormalities, or incomplete KD. This may be explained that chemerin was mainly involved in the process of immune regulation and inflammatory activation in acute KD, also might be associated with the inflammatory infiltration of vascular endothelial cells. However, the pathogenesis of chemerin in coronary artery lesions of patients with KD still need to be confirmed further.
Omentin-1 or interlectin-1, a new cDNA expressed specifically in omental adipose tissue, was a new adipocytokine identified by Schäffler et al. [
37]. Omentin has been reported to enhance insulin-mediated glucose-uptake in adipocytes and to activate protein kinase Akt/PKB, which was also named insulin sensitizer [
38,
39]. It is well known that immune vasculitis is the most characteristic pathologic change in KD patients, especially targeting coronary arteries among small and medium vessels. It has been conducted that the omentin-1 levels in synovial joints of patients with rheumatoid arthritis(RA) were lower than those of patients with osteoarthritis(OA) [
38]. Patients with obesity showed decreased omentin-1 levels compared with thin patients and they suggested that omentin-1 levels may be predictive of the metabolic consequences or co-morbidities associated with obesity [
39,
40]. In this study, we found that serum levels of omentin-1 as a kind of anti-inflammatory adipokine decreased in the acute stage of patients with KD, which could be explained that omentin-1 as a protective factor might play vital roles in anti-inflammation and inhibition the activation of endothelial cells at lower levels. What’s more interesting is that we found circulating omentin-1 levels are positively correlated with total cholesterol, which is a crucial biomarker of lipid metabolism. Lower omentin-1 may be beneficial to correct the disorders of lipid metabolism through inhibiting oxLDL-induced foam cell formation and protecting vascular endothelial cells from inflammatory lesions and alleviating vascular injuries of KD [
41]. However, Antonella et al. [
19] reported that serum omentin levels were higher in KD patients than healthy controls. This result is just inconsistent with our results. This difference may be explained through the following three possible reasons. Firstly, omentin has two different subtypes, which might play different roles in the development of KD. In this study, we focus on the correlation between omentin-1 and acute KD. But it is not clear Antonella and his or her colleague studied which subtype or total in their article. Secondly, we found that omentin-1 in the KD group was lower than febrile controls but not than healthy controls. However, Antonella et al. reported that serum omentin levels were significantly higher in KD patients versus healthy controls. Lastly, we have to take individual differences from different regions into account. In short, these controversial results need to be further confirmed by numerous studies.
Adiponectin is the most abundant adipokine secreted by adipocytes, which exhibits multiple physiological functions through combined to its receptors. In vitro and vivo experiments showed that adiponectin treatment attenuates lipopolysaccharide (LPS)-induced expression of TNF-a in cultured macrophages through inhibition of NF-κB signaling and overexpression of adiponectin alleviates progression of atherosclerotic lesions in apolipoprotein E knockout (KO) mice, with an accompanying decrease in TNF-a and SR-A expression [
42,
43]. Here, this research indicates that adiponectin levels reduced significantly in the acute phase of KD, as with omentin-1 levels. The possible pathogenesis might be that adiponectin plays anti-inflammatory roles through blockade of NF-κB signaling and further inhibits the production of proinflammatory cytokines such as TNF-α and MMP-12 [
44,
45]. It is worth noting that TNF-α is still overexpressed in the acute phase of KD might because that it has exceeded the inhibition properties of adiponectin though this still needs to be explored through in vitro and vivo experiments. In addition, we didn’t discover any pieces of evidence to support the correlation between adiponectin and coronary artery abnormalities of KD.
Recently, there were some case reports and a clinical trial reported that IL-1 receptor inhibitors (IL-1RA) improve the pathogenetic condition of KD with the restoration of dilation coronary artery [
46‐
49]. We found that a low level of IL-1β in KD patients accompanied coronary artery lesions(CAL). This suggests that IL-1β is involved in the progress of coronary artery lesions, which implied that IL-1βbecome a new target in the treatment of KD or KD accompanied CAL in the future. This study provides evidence for this conclusion.
Ishwarlal et al. reported that lower levels of omentin-1 and higher levels of chemerin in nascent metabolic syndrome were risk factors for diabetes and cardiovascular disease [
50]. In this study, disorders of lipid metabolism are present in patients with acute KD, which is consistent with the results reported previously [
51]. We also found that serum levels of omentin-1 were positively correlated with both chemerin and total cholesterol, which suggests that chemerin and omentin-1 are directly or indirectly closely related to disorders of lipid metabolism in the acute phase of KD. Adipokines were involved in lipid metabolism in autocrine or paracrine manners and these disorders of lipids metabolism may be associated with inappropriate secretion of omentin-1 and chemerin which suggests that low levels of omentin-1 and chemerin may predict disorders of lipid metabolism, although this still needs to be supported by a larger data sample. Interestingly, it showed that hemoglobin in acute KD was higher than febrile control, which implied that hemoglobin was related to inflammation state in acute KD. Kim et al. [
18]. described that resistin and serum IL-6 were significantly elevated and hemoglobin significantly lower in KD patients with coronary anomalies. Hemoglobin levels were negatively correlated with resistin levels in KD patients. However, it’s a pity that we didn’t find the correlation between hemoglobin and adipokines including chemerin, omentin-1, and adiponectin.
However, our study has several limitations. Firstly, we concentrated on the influences of each adipokine on KD respectively but cannot evaluate the interrelationship among different adipokines. Moreover, we only analyzed levels of adipokines including chemerin, omentin-1 and adiponectin in the acute stage without tracking the changing process of adipokines during the full development of KD and without further studying mechanism of action. Finally, our data are confined to a limited number of samples and concentricity of the region, thus further research with a larger sample and covering multi-centers will be more persuasive.