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Erschienen in: Clinical and Experimental Nephrology 3/2011

01.06.2011 | Original Article

Cisplatin induces Sirt1 in association with histone deacetylation and increased Werner syndrome protein in the kidney

verfasst von: Yukitoshi Sakao, Akihiko Kato, Takayuki Tsuji, Hideo Yasuda, Akashi Togawa, Yoshihide Fujigaki, Tomoaki Kahyo, Mitsutoshi Setou, Akira Hishida

Erschienen in: Clinical and Experimental Nephrology | Ausgabe 3/2011

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Abstract

Background

Sirt1, a mammalian homolog of silent information regulator 2 (Sir2), is the founding member of class III histone deacetylase (HDAC).

Methods

In this study, we examined whether Sirt1 is involved in the modification of acetylated histone H3, acetylated p53 and Werner syndrome protein (WRN), which is stabilized by Sirt1-mediated deacetylation, in cisplatin (CDDP)-induced acute renal failure (ARF) in rats.

Results

Administration of CDDP (5 mg/kg body weight) caused an increase in the Sirt1 protein level by 6 h; this increase peaked at day 5 and declined until day 14. Sirt1 was induced to a greater extent in rats with severe ARF. In contrast, HDAC3 and HDAC5 were not induced within 24 h after CDDP administration. The level of acetylated histone H3 in the kidney decreased early, i.e., at 6 h, and was minimal at day 5, after which the level gradually increased by day 14. CDDP marginally induced acetylated p53 within 24 h after administration. Increased WRN also became evident at 6 h, and continued to be upregulated until day 5, accompanied by an increase in proliferating cell nuclear antigen (PCNA). Transfection of Sirt1 to human embryonic kidney 293 cells mitigated the CDDP-induced cellular damage.

Conclusions

These findings collectively suggest that CDDP increases the level of Sirt1 protein in the kidneys in association with histone H3 deacetylation and increased WRN and PCNA production. The induced Sirt1 may work defensively to mitigate CDDP-induced tubular damage by inactivating core histone transcriptionally, and by repairing DNA damage.
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Metadaten
Titel
Cisplatin induces Sirt1 in association with histone deacetylation and increased Werner syndrome protein in the kidney
verfasst von
Yukitoshi Sakao
Akihiko Kato
Takayuki Tsuji
Hideo Yasuda
Akashi Togawa
Yoshihide Fujigaki
Tomoaki Kahyo
Mitsutoshi Setou
Akira Hishida
Publikationsdatum
01.06.2011
Verlag
Springer Japan
Erschienen in
Clinical and Experimental Nephrology / Ausgabe 3/2011
Print ISSN: 1342-1751
Elektronische ISSN: 1437-7799
DOI
https://doi.org/10.1007/s10157-011-0421-5

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