Skip to main content
main-content

27.02.2019 | Original Article | Ausgabe 4/2019

Comparative Clinical Pathology 4/2019

Clinical observation, acute phase protein levels, and histopathological changes of mammary gland in experimentally infected goats with Staphylococcus aureus

Zeitschrift:
Comparative Clinical Pathology > Ausgabe 4/2019
Autoren:
M. B. Sadiq, R. Mansor, S. S. Syed-Hussain, A. A. Saharee, Z. Zakaria, A. A. Syahirah, I. Bousnane, Z. A. Jaezah Adlina, A. Salleh, W. I. Wan Mohd Sukri, F. Mustaffa-Kamal, S. Z. Ramanoon
Wichtige Hinweise

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Abstract

Staphylococcus aureus (S. aureus) is a major cause of mastitis in small ruminants, and studies on the potential application of acute phase proteins (APPs) in disease biomarkers and understanding the disease mechanism in mastitis affecting small ruminants are limited. Thus, the objective of this study was to determine clinical, histopathological changes and serum concentrations of serum amyloid A (SAA) and haptoglobin (Hp) associated with experimentally induced mastitis using S. aureus. Left mammary quarter in the does was challenged with sterile Ringers buffered salt solution as group 1 (control animals; n = 6) and S. aureus (1.5 × 108 cfu/ml) as group 2 (inoculated does; n = 5) respectively. The animals were monitored at various time intervals for the signs of mastitis and serum concentrations of Hp and SAA. Majority of the animals in the inoculated group showed typical signs of acute mastitis characterized with fever, warm, painful, and swollen mammary gland as early as 24-h post-inoculation (PI). There was significant increased (p < 0.05) in the levels of Hp and SAA in the serum of inoculated animals at 24-h post-inoculation. Histopathological changes in the mammary gland of the inoculated goats included generalized and severe infiltration of the interstitial spaces with polymorphonuclear neutrophils (PMN), severe necrosis of secretory cells, and hyperplastic epithelial and luminal lining. In conclusion, inoculation of S. aureus intramammarily leads to an increment of Hp and SAA at 24-h PI which indicates that both APPs played crucial role as biomarkers in detection and monitoring of S. aureus mastitis in goats.

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

★ PREMIUM-INHALT
e.Med Interdisziplinär

Mit e.Med Interdisziplinär erhalten Sie Zugang zu allen CME-Fortbildungen und Fachzeitschriften auf SpringerMedizin.de. Zusätzlich können Sie eine Zeitschrift Ihrer Wahl in gedruckter Form beziehen – ohne Aufpreis.

Jetzt e.Med bestellen und 100 € sparen!

Literatur
Über diesen Artikel

Weitere Artikel der Ausgabe 4/2019

Comparative Clinical Pathology 4/2019 Zur Ausgabe

Neu im Fachgebiet Pathologie

01.02.2020 | Forensische Begutachtung | CME | Ausgabe 1/2020

Diagnose einer gewaltsamen Erstickung

Teil 1: Reevaluation der Spezifität makroskopischer und histomorphologischer Befunde

27.01.2020 | Schwerpunkt: Lunge | Ausgabe 1/2020

Immunhistochemiebasierte prädiktive Biomarker bei Lungenkarzinomen

27.01.2020 | Schwerpunkt: Lunge | Ausgabe 1/2020

Zytologie der primären Lungenkarzinome