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18.03.2021 | Translational Research

Clinical Outcomes and Immune Metrics in Intratumoral Basophil-Enriched Gastric Cancer Patients

verfasst von: Xudong He, PhD, Yifan Cao, MD, Yun Gu, MD, Hanji Fang, MD, Jieti Wang, MD, Xin Liu, PhD, Kunpeng Lv, PhD, Kuan Yu, MD, Yuchao Fei, MD, Chao Lin, MD, Hao Liu, MD, Heng Zhang, MD, He Li, MD, Jiejie Xu, MD, Ruochen Li, MD, Hongyong He, MD

Erschienen in: Annals of Surgical Oncology | Ausgabe 11/2021

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Abstract

Background

Accumulation of basophils has been reported in several malignancies. In gastric cancer, the relation between tumor-infiltrating basophils and patient overall survival and chemotherapeutic responsiveness still remains obscure.

Objective

We aimed to investigate the postoperative prognostic and predictive significance of basophils to survival outcomes and chemotherapeutic responsiveness in resectable gastric cancer.

Methods

The study enrolled two independent patient data sets with 448 gastric cancer patients overall. Basophils were evaluated with the use of immunohistochemistry (IHC) staining, and the correlation with clinicopathological characteristics, survival outcomes, and responsiveness to fluorouracil-based adjuvant chemotherapy (ACT) were investigated. Additionally, IHC was applied to characterize immune contexture in gastric cancer.

Results

In either the discovery or validation data sets, accumulated basophils indicated poorer prognosis, and tumor-infiltrating basophils were identified as an independent adverse prognostic factor by multivariate analysis. Furthermore, tumor-infiltrating basophils determined significantly inferior therapeutic responsiveness to fluorouracil-based ACT in patients with stage III tumors. In addition, the abundance of basophils was correlated with an immunoevasive contexture characterized by M2-polarized macrophage infiltration. Moreover, our findings indicated elevated interleukin-4 expression but decreased interferon-γ expression in the high-basophils subgroup.

Conclusions

Tumor-infiltrating basophils in gastric cancer were identified as an independent adverse prognosticator, and also predicted inferior chemotherapeutic responsiveness, which identified those patients in need of much more individualized postoperative adjuvant therapy and more stringent follow-up. Furthermore, the infiltration of basophils was associated with immunoevasive tumor microenvironment, which might be a potential immunotherapeutic target for gastric cancer.

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Titel: Clinical Outcomes and Immune Metrics in Intratumoral Basophil-Enriched Gastric Cancer Patients
verfasst von: Xudong He, PhD
Yifan Cao, MD
Yun Gu, MD
Hanji Fang, MD
Jieti Wang, MD
Xin Liu, PhD
Kunpeng Lv, PhD
Kuan Yu, MD
Yuchao Fei, MD
Chao Lin, MD
Hao Liu, MD
Heng Zhang, MD
He Li, MD
Jiejie Xu, MD
Ruochen Li, MD
Hongyong He, MD
Publikationsdatum: 18.03.2021
Verlag: Springer International Publishing
Erschienen in: Annals of Surgical Oncology / Ausgabe 11/2021
Print ISSN: 1068-9265
Elektronische ISSN: 1534-4681
DOI: https://doi.org/10.1245/s10434-021-09815-0

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Clinical Outcomes and Immune Metrics in Intratumoral Basophil-Enriched Gastric Cancer Patients