Background
Methods
Tissue samples
Detection of mRNA expression levels
UGT1A1 polymorphism analysis
Statistical analysis
Results
Patient characteristics
Characteristic | Cases, n (%) |
---|---|
Gender | |
Male | 76 (77.6) |
Female | 22 (22.4) |
Age | |
< 57 years | 41 (41.8) |
> 57 years | 57 (58.2) |
Family history | |
Yes | 5 (5.1) |
No | 93 (94.9) |
Alcohol history | |
Yes | 32 (32.7) |
No | 66 (67.3) |
Histological type | |
Tubular adenocarcinoma | 75 (76.5) |
Non-tubular adenocarcinoma | 23 (23.5) |
Differentiation | |
Undifferentiated | 15 (15.3) |
Poor | 60 (61.2) |
Moderate | 22 (22.4) |
Well | 1 (1.0) |
Growth patterns | |
Expansive | 2 (2.0) |
Invasive | 71 (72.4) |
Expansive + invasive | 25 (25.5) |
Depth of invasion | |
T1 | 14 (14.3) |
T2 | 5 (5.1) |
T3 | 3 (3.1) |
T4 | 76 (77.6) |
Lymph node metastasis | |
N0 | 52 (53.1) |
N1 | 32 (32.7) |
N2 | 11 (11.2) |
Undetermined | 3 (3.1) |
Distant metastasis | |
M0 | 86 (87.8) |
M1 | 12 (12.2) |
TNM staging | |
I | 18 (18.4) |
IIA-IIC | 29 (29.6) |
IIIA-IIIC | 39 (39.8) |
IVA-IVB | 12 (12.2) |
Gene expression and their relationships with clinical characteristics
Clinical features | ERCC1 | BRCA1 | TYMS | RRM1 | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Low | Middle | High | Low | Middle | High | Low | Middle | High | Low | Middle | High | |
Gender | ||||||||||||
Male | 11 (14.5) | 46 (60.5) | 19 (25.0) | 6 (7.9) | 39 (51.3) | 31 (40.8) | 24 (31.6) | 36 (47.4) | 16 (21.1) | 45 (31.6) | 25 (31.6) | 6 (31.6) |
Female | 6 (27.3) | 8 (36.4) | 8 (36.4) | 3 (13.6) | 11 (50.0) | 8 (36.4) | 6 (27.3) | 9 (40.9) | 7 (31.8) | 7 (31.6) | 11 (31.6) | 4 (31.6) |
χ
2, P
| 4.219, .219 | 0.702, 0.704 | 1.101, 0.577 | 5.541, 0.063 | ||||||||
Age | ||||||||||||
< 57 years | 7 (17.1) | 19 (46.3) | 15 (36.6) | 6 (14.6) | 22 (53.7) | 13 (31.7) | 18 (43.9) | 14 (34.1) | 9 (22.0) | 20 (48.8) | 19 (46.3) | 2 (4.9) |
> 57 years | 10 (17.5) | 35 (61.4) | 12 (21.1) | 3 (5.3) | 28 (49.1) | 26 (45.6) | 12 (21.1) | 31 (54.4) | 14 (24.6) | 32 (56.1) | 17 (29.8) | 8 (14.0) |
χ
2, P
| 3.073, 0.215 | 3.535, 0.171 | 6.264, 0.044
| 3.974, 0.137 | ||||||||
Family history | ||||||||||||
Yes | 0 (0.0) | 3 (60.0) | 2 (40.0) | 1 (20.0) | 3 (60.0) | 1 (20.0) | 2 (40.0) | 2 (40.0) | 1 (20.0) | 2 (40.0) | 3 (60.0) | 0 (0.0) |
No | 17 (18.3) | 51 (54.8) | 25 (26.9) | 8 (8.6) | 47 (50.5) | 38 (40.9) | 28 (30.1) | 43 (46.2) | 22 (23.7) | 50 (53.8) | 33 (35.5) | 10 (10.8) |
χ
2, P
| 2.065, 0.356 | 1.219, 0.544 | 0.209, 0.901 | 1.889, 0.389 | ||||||||
Alcohol history | ||||||||||||
Yes | 6 (18.8) | 18 (56.3) | 8 (25.0) | 2 (6.3) | 15 (46.9) | 15 (46.9) | 7 (21.9) | 17 (53.1) | 8 (25.0) | 15 (46.9) | 14 (43.8) | 3 (9.4) |
No | 11 (16.7) | 36 (54.5) | 19 (28.8) | 7 (10.6) | 35 (53.0) | 24 (36.4) | 23 (34.8) | 28 (42.4) | 15 (22.7) | 37 (56.1) | 22 (33.3) | 7 (10.6) |
χ
2, P
| 0.178, 0.915 | 1.204, 0.548 | 1.770, 0.413 | 1.011, 0.603 | ||||||||
Histological type | ||||||||||||
Tubular adenocarcinoma | 14 (18.7) | 40 (53.3) | 21 (28.0) | 6 (8.0) | 37 (49.3) | 32 (42.7) | 20 (26.7) | 36 (48.0) | 19 (25.3) | 40 (53.3) | 26 (34.7) | 9 (12.0) |
Non-tubular adenocarcinoma | 3 (13.0) | 14 (60.9) | 6 (26.1) | 3 (13.0) | 13 (56.5) | 7 (30.4) | 10 (43.5) | 9 (39.1) | 4 (17.4) | 12 (52.2) | 10 (43.5) | 1 (4.3) |
χ
2, P
| 0.526, 0.769 | 1.328, 0.515 | 2.400, 0.301 | 1.387, 0.500 | ||||||||
Differentiation | ||||||||||||
Undifferentiated | 2 (13.3) | 8 (53.3) | 5 (33.3) | 2 (13.3) | 8 (53.3) | 5 (33.3) | 7 (46.7) | 5 (33.3) | 3 (20.0) | 7 (46.7) | 8 (53.3) | 0 (0.0) |
Poor | 10 (16.7) | 33 (55.0) | 17 (28.3) | 4 (6.7) | 32 (53.3) | 24 (40.0) | 15 (25.0) | 31 (51.7) | 14 (23.3) | 31 (51.7) | 23 (38.3) | 6 (10.0) |
Moderate | 4 (18.2) | 13 (59.1) | 5 (22.7) | 3 (13.6) | 10 (45.5) | 9 (40.9) | 8 (36.4) | 9 (40.9) | 5 (22.7) | 14 (63.6) | 5 (22.7) | 3 (13.6) |
Well | 1 (100.0) | 0 (0.0) | 0 (0.0) | 0 (0.0) | 0 (0.0) | 1 (100.0) | 0 (0.0) | 0 (0.0) | 1 (100.0) | 0 (0.0) | 0 (0.0) | 1 (100.0) |
χ
2, P
| 4.132, 0.659 | 3.350, 0.764 | 6.067, 0.416 | 10.825, 0.094 | ||||||||
Growth patterns | ||||||||||||
Expansive | 0 (0.0) | 0 (0.0) | 2 (100.0) | 0 (0.0) | 0 (0.0) | 2 (100.0) | 2 (100.0) | 0 (0.0) | 0 (0.0) | 1 (50.0) | 1 (50.0) | 0 (0.0) |
Invasive | 11 (15.5) | 41 (57.7) | 19 (26.8) | 7 (9.9) | 34 (47.9) | 30 (42.3) | 18 (25.4) | 33 (46.5) | 20 (28.2) | 34 (47.9) | 30 (42.3) | 7 (9.9) |
Expansive + invasive | 6 (24.0) | 13 (52.0) | 6 (24.0) | 2 (8.0) | 16 (64.0) | 7 (28.0) | 10 (40.0) | 12 (48.0) | 3 (12.0) | 17 (68.0) | 5 (20.0) | 3 (12.0) |
χ
2, P
| 6.141 | 0.189 | 5.744 | 0.219 | 8.438 | 0.077 | 4.765 | 0.312 | ||||
Depth of invasion | ||||||||||||
T1 | 2 (14.3) | 5 (35.7) | 7 (50.0) | 1 (7.1) | 8 (57.1) | 5 (35.7) | 7 (50.0) | 4 (28.6) | 3 (21.4) | 9 (64.3) | 4 (28.6) | 1 (7.1) |
T2 | 2 (40.0) | 2 (40.0) | 1 (20.0) | 0 (0.0) | 2 (40.0) | 3 (60.0) | 2 (40.0) | 2 (40.0) | 1 (20.0) | 2 (40.0) | 2 (40.0) | 1 (20.0) |
T3 | 1 (33.3) | 2 (66.6) | 0 (0.0) | 0 (0.0) | 0 (0.0) | 3 (100.0) | 0 (0.0) | 1 (33.3) | 2 (66.6) | 2 (66.6) | 0 (0.0) | 1 (33.3) |
T4 | 12 (15.8) | 45 (59.2) | 19 (25.0) | 8 (10.5) | 40 (52.6) | 28 (36.8) | 21 (27.6) | 38 (50.0) | 17 (22.4) | 39 (51.3) | 30 (39.5) | 7 (9.2) |
χ
2, P
| 7.241 | 0.299 | 7.599 | 0.269 | 6.797 | 0.340 | 4.839 | 0.621 | ||||
Lymph node metastasis | ||||||||||||
N0 | 10 (19.2) | 28 (53.8) | 14 (26.9) | 3 (5.8) | 30 (57.7) | 19 (36.5) | 18 (34.6) | 22 (42.3) | 12 (23.1) | 29 (55.8) | 19 (36.5) | 4 (7.7) |
N1 | 5 (15.6) | 18 (56.3) | 9 (28.1) | 3 (9.4) | 14 (43.8) | 15 (46.9) | 8 (25.0) | 15 (46.9) | 9 (28.1) | 14 (43.8) | 15 (46.9) | 3 (9.4) |
N2 | 1 (9.1) | 7 (63.6) | 3 (27.3) | 2 (18.2) | 6 (54.5) | 3 (27.3) | 3 (27.3) | 7 (63.6) | 1 (9.1) | 6 (54.5) | 2 (18.2) | 3 (27.3) |
Undetermined | 1 (33.3) | 1 (33.3) | 1 (33.3) | 1 (33.3) | 0 (0.0) | 2 (66.7) | 1 (33.3) | 1 (33.3) | 1 (33.3) | 3 (100.0) | 0 (0.0) | 0 (0.0) |
χ
2, P
| 1.538 | 0.957 | 7.979 | 0.240 | 3.262 | 0.775 | 9.043 | 0.172 | ||||
Distant metastasis | ||||||||||||
M0 | 15 (17.4) | 48 (55.8) | 23 (26.7) | 7 (8.1) | 45 (52.3) | 34 (39.5) | 28 (32.6) | 38 (44.2) | 20 (23.3) | 44 (51.2) | 33 (38.4) | 9 (10.5) |
M1 | 2 (16.7) | 6 (50.0) | 4 (33.3) | 2 (16.7) | 5 (41.7) | 5 (41.7) | 2 (16.7) | 7 (58.3) | 3 (25.0) | 8 (66.7) | 3 (25.0) | 1 (8.3) |
χ
2, P
| 0.227, 0.893 | 0.954, 0.621 | 1.460, 0.482 | 1.065, 0.587 | ||||||||
TNM staging | ||||||||||||
I | 4 (22.2) | 6 (33.3) | 8 (44.4) | 1 (5.6) | 10 (55.6) | 7 (38.9) | 9 (50.0) | 5 (27.8) | 4 (22.2) | 11 (61.1) | 5 (27.8) | 2 (11.1) |
IIA-IIC | 5 (17.2) | 19 (65.5) | 5 (17.2) | 2 (6.9) | 16 (55.2) | 11 (37.9) | 7 (24.1) | 15 (51.7) | 7 (24.1) | 16 (55.2) | 11 (37.9) | 2 (6.9) |
IIIA-IIIC | 6 (15.4) | 23 (59.0) | 10 (25.6) | 4 (10.3) | 19 (48.7) | 16 (41.0) | 12 (30.8) | 18 (46.2) | 9 (23.1) | 17 (43.6) | 17 (43.6) | 5 (12.8) |
IVA-IVB | 2 (16.7) | 6 (50.0) | 4 (33.3) | 2 (16.7) | 5 (41.7) | 5 (41.7) | 2 (16.7) | 7 (58.3) | 3 (25.0) | 8 (66.7) | 3 (25.0) | 3 (8.3) |
χ
2, P
| 5.876, 0.437 | 1.598, 0.953 | 5.308, 0.505 | 3.393, 0.758 |
Clinical features | TUBB3 | STMN1 | TOP2A | ||||||
---|---|---|---|---|---|---|---|---|---|
Low | Middle | High | Low | Middle | High | Low | Middle | High | |
Gender | |||||||||
Male | 22 (28.9) | 41 (53.9) | 13 (17.1) | 27 (35.5) | 36 (47.4) | 13 (17.1) | 9 (11.8) | 45 (59.2) | 22 (28.9) |
Female | 13 (59.1) | 8 (36.4) | 1 (4.5) | 7 (31.8) | 12 (54.5) | 3 (13.6) | 5 (22.7) | 11 (80.0) | 6 (27.3) |
χ
2, P
| 7.280, 0.026
| 0.373, 0.830 | 1.685, 0.431 | ||||||
Age | |||||||||
< 57 years | 21 (51.2) | 16 (39.0) | 4 (9.8) | 19 (46.3) | 19 (46.3) | 3 (7.3) | 8 (19.5) | 24 (58.5) | 9 (22.0) |
> 57 years | 14 (24.6) | 33 (57.9) | 10 (17.5) | 15 (26.3) | 29 (50.9) | 13 (22.8) | 6 (10.5) | 32 (56.1) | 19 (33.3) |
χ
2, P
| 7.464, 0.024
| 6.361, 0.042
| 2.453, 0.293 | ||||||
Family history | |||||||||
Yes | 4 (80.0) | 0 (0.0) | 1 (20.0) | 2 (40.0) | 3 (60.0) | 0 (0.0) | 2 (40.0) | 2 (40.0) | 1 (20.0) |
No | 31 (33.3) | 49 (52.7) | 13 (14.0) | 32 (34.4) | 45 (48.4) | 16 (17.2) | 12 (12.9) | 54 (58.1) | 27 (29.0) |
χ
2, P
| 7.414, 0.025
| 1.839, 0.399 | 2.128, 0.345 | ||||||
Alcohol history | |||||||||
Yes | 10 (31.3) | 17 (53.1) | 5 (15.6) | 12 (37.5) | 13 (40.6) | 7 (21.9) | 3 (9.4) | 18 (56.3) | 11 (34.4) |
No | 25 (37.9) | 32 (48.5) | 9 (13.6) | 22 (33.3) | 35 (53.0) | 9 (13.6) | 11 (16.7) | 38 (57.6) | 17 (25.8) |
0.418, 0.812 | 1.681, 0.432 | 1.369, 0.504 | |||||||
Histological type | |||||||||
Tubular adenocarcinoma | 29 (38.7) | 34 (45.3) | 12 (16.0) | 23 (30.7) | 38 (50.7) | 14 (18.7) | 10 (13.3) | 40 (53.3) | 25 (33.3) |
Non-tubular adenocarcinoma | 6 (26.1) | 15 (65.2) | 2 (8.7) | 11 (47.8) | 10 (43.5) | 2 (8.7) | 4 (17.4) | 16 (69.6) | 3 (13.0) |
χ
2, P
| 2.829, 0.243 | 0.751, 0.253 | 3.551, 0.169 | ||||||
Differentiation | |||||||||
Undifferentiated | 5 (33.3) | 10 (66.7) | 0 (0.0) | 8 (53.3) | 6 (40.0) | 1 (6.7) | 3 (20.0) | 9 (60.0) | 3 (20.0) |
Poor | 20 (33.3) | 29 (48.3) | 11 (18.3) | 19 (31.7) | 32 (53.3) | 9 (15.0) | 7 (11.7) | 34 (56.7) | 19 (31.7) |
Moderate | 9 (40.9) | 10 (45.5) | 3 (13.6) | 7 (31.8) | 10 (45.5) | 5 (22.7) | 4 (18.2) | 13 (59.1) | 5 (22.7) |
Well | 1 (100.0) | 0 (0.0) | 0 (0.0) | 0 (0.0) | 0 (0.0) | 1 (100.0) | 0 (0.0) | 0 (0.0) | 1 (100.0) |
χ
2, P
| 8.144, 0.228 | 7.341, 0.290 | 4.279, 0.639 | ||||||
Growth patterns | |||||||||
Expansive | 1 (50.0) | 1 (50.0) | 0 (0.0) | 2 (100.0) | 0 (0.0) | 0 (0.0) | 1 (50.0) | 1 (50.0) | 0 (0.0) |
Invasive | 24 (33.8) | 34 (47.9) | 13 (18.3) | 21 (29.6) | 36 (50.7) | 14 (19.7) | 9 (12.7) | 42 (59.2) | 20 (28.2) |
Expansive + invasive | 10 (40.0) | 14 (56.0) | 1 (4.0) | 10 (40.0) | 11 (44.0) | 12 (48.0) | 4 (16.0) | 13 (52.0) | 8 (32.0) |
χ
2, P
| 4.443, 0.349 | 7.200, 0.126 | 2.694, 0.610 | ||||||
Metastasis | |||||||||
Yes | 14 (26.9) | 29 (55.8) | 9 (17.3) | 16 (30.8) | 26 (50.0) | 10 (19.2) | 5 (9.6) | 30 (57.7) | 17 (32.7) |
No | 21 (45.7) | 20 (43.5) | 5 (10.9) | 18 (39.1) | 22 (47.8) | 6 (13.0) | 9 (19.6) | 26 (56.5) | 11 (23.9) |
χ
2, P
| 3.843, 0.146 | 1.088, 0.581 | 2.356, 0.308 | ||||||
Depth of invasion | |||||||||
T1 | 6 (42.9) | 8 (57.1) | 0 (0.0) | 5 (35.7) | 8 (57.1) | 1 (7.1) | 5 (35.7) | 5 (35.7) | 4 (28.6) |
T2 | 2 (40.0) | 2 (40.0) | 1 (20.0) | 1 (20.0) | 2 (40.0) | 2 (40.0) | 0 (0.0) | 4 (80.0) | 1 (20.0) |
T3 | 1 (33.3) | 1 (33.3) | 1 (33.3) | 0 (0.0) | 1 (33.3) | 2 (66.6) | 0 (0.0) | 1 (33.3) | 2 (66.6) |
T4 | 26 (34.2) | 38 (50.0) | 12 (15.8) | 28 (36.8) | 37 (48.7) | 11 (14.5) | 9 (11.8) | 46 (60.5) | 21 (27.6) |
χ
2, P
| 5.469, 0.485 | 7.900, 0.246 | 9.263, 0.159 | ||||||
Lymph node metastasis | |||||||||
N0 | 22 (42.3) | 25 (48.1) | 5 (9.6) | 20 (38.5) | 26 (50.0) | 6 (11.5) | 9 (17.3) | 31 (59.6) | 12 (23.1) |
N1 | 6 (18.8) | 19 (59.4) | 7 (21.9) | 10 (31.3) | 14 (43.8) | 8 (25.0) | 4 (12.8) | 17 (53.1) | 11 (34.4) |
N2 | 5 (45.5) | 4 (36.4) | 2 (18.2) | 4 (36.4) | 5 (45.5) | 2 (18.2) | 0 (0.0) | 8 (72.7) | 3 (27.3) |
Undetermined | 2 (66.7) | 1 (33.3) | 0 (0.0) | 0 (0.0) | 3 (100.0) | 0 (0.0) | 1 (33.3) | 0 (0.0) | 2 (66.7) |
χ
2, P
| 8.812, 0.184 | 6.938, 0.327 | 10.139, 0.119 | ||||||
Distant metastasis | |||||||||
M0 | 31 (36.0) | 41 (51.2) | 11 (12.8) | 33 (38.4) | 39 (45.3) | 14 (16.3) | 12 (14.0) | 51 (59.3) | 23 (26.7) |
M1 | 4 (33.3) | 5 (41.7) | 3 (25.0) | 1 (8.3 | 9 (75.0) | 2 (16.7) | 2 (16.7) | 5 (41.7) | 5 (41.7) |
χ
2, P
| 1.148, 0.563 | 5.461, 0.065 | 1.410, 0.494 | ||||||
TNM staging | |||||||||
I | 8 (44.4) | 9 (50.0) | 1 (5.6) | 6 (33.3) | 9 (50.0) | 3 (16.7) | 5 (27.8) | 8 (44.4) | 5 (27.8) |
IIA-IIC | 11 (37.9) | 15 (51.7) | 3 (10.3) | 11 (37.9) | 15 (51.7) | 3 (10.3) | 3 (10.3) | 20 (69.0) | 6 (20.7) |
IIIA-IIIC | 12 (30.8) | 20 (51.3) | 7 (17.9) | 16 (41.0) | 15 (38.5) | 8 (20.5) | 4 (10.3) | 23 (59.0) | 12 (30.8) |
IVA-IVB | 4 (33.3) | 5 (41.7) | 3 (25.0) | 1 (8.3) | 9 (75.0) | 2 (16.7) | 2 (16.7) | 5 (41.7) | 5 (41.7) |
χ
2, P
| 3.629, 0.727 | 7.539, 0.274 | 5.869, 0.438 |
UGT1A1 polymorphisms and their relationships with clinical characteristics
Clinical features | *28 | −3156 GA | 211GA | ||||||
---|---|---|---|---|---|---|---|---|---|
TA 6/6 | TA 6/7 | TA 7/7 | GG | GA | AA | GG | GA | AA | |
Gender | |||||||||
Male | 64 (84.2) | 10 (13.2) | 2 (2.6) | 64 (84.2) | 10 (13.2) | 2 (2.6) | 49 (64.5) | 23 (30.3) | 4 (5.3) |
Female | 17 (77.3) | 5 (22.7) | 0 (0.0) | 17 (77.3) | 5 (22.7) | 0 (0.0) | 13 (59.1) | 9 (40.9) | 0 (0.0) |
χ
2, P
| 2.046, 0.359 | 2.046, 0.359 | 2.674, 0.263 | ||||||
Age | |||||||||
< 57 years | 33 (80.5) | 8 (19.5) | 0 (0.0) | 33 (80.5) | 8 (19.5) | 0 (0.0) | 26 (63.4) | 14 (34.1) | 1 (2.4) |
> 57 years | 48 (84.2) | 7 (12.3) | 2 (3.5) | 48 (84.2) | 7 (12.3) | 2 (3.5) | 36 (63.2) | 18 (31.6) | 3 (5.3) |
χ
2, P
| 3.009, 0.222 | 3.009, 0.222 | 0.544, 0.762 | ||||||
Family history | |||||||||
Yes | 4 (80.0) | 1 (20.0) | 0 (0.0) | 4 (80.0) | 1 (20.0) | 0 (0.0) | 2 (40.0) | 2 (40.0) | 1 (20.0) |
No | 77 (82.8) | 14 (15.1) | 2 (2.2) | 77 (82.8) | 14 (15.1) | 2 (2.2) | 60 (64.5) | 30 (62.3) | 3 (3.2) |
χ
2, P
| 0.283, 0.868 | 0.283, 0.868 | 2.364, 0.307 | ||||||
Alcohol history | |||||||||
Yes | 29 (90.6) | 3 (9.4) | 0 (0.0) | 29 (90.6) | 3 (9.4) | 0 (0.0) | 21 (65.6) | 9 (28.1) | 2 (6.3) |
No | 52 (78.8) | 12 (18.2) | 2 (3.0) | 52 (78.8) | 12 (18.2) | 2 (3.0) | 41 (62.1) | 23 (34.8) | 2 (3.0) |
χ
2, P
| 3.132, 0.209 | 3.132, 0.209 | 0.861, 0.650 | ||||||
Histological type | |||||||||
Tubular adenocarcinoma | 60 (80.0) | 13 (17.3) | 2 (2.7) | 60 (80.0) | 13 (17.3) | 2 (2.7) | 54 (72.0) | 19 (25.3) | 2 (2.7) |
Non-tubular adenocarcinoma | 21 (91.3) | 2 (8.7) | 0 (0.0) | 21 (91.3) | 2 (8.7) | 0 (0.0) | 8 (34.8) | 13 (56.5) | 2 (8.7) |
χ
2, P
| 2.308, 0.315 | 2.308, 0.315 | 10.339, 0.006
| ||||||
Differentiation | |||||||||
Undifferentiated | 13 (86.7) | 2 (13.3) | 0 (0.0) | 13 (86.7) | 2 (13.3) | 0 (0.0) | 6 (40.0) | 7 (46.7) | 2 (13.3) |
Poor | 51 (85.0) | 8 (13.3) | 1 (1.7) | 51 (85.0) | 8 (13.3) | 1 (1.7) | 41 (68.3) | 18 (30.0) | 1 (1.7) |
Moderate | 17 (77.3) | 4 (18.2) | 1 (4.5) | 17 (77.3) | 4 (18.2) | 1 (4.5) | 14 (63.6) | 7 (31.8) | 1 (4.5) |
Well | 0 (0.0) | 1 (100.0) | 0 (0.0) | 0 (0.0) | 1 (100.0) | 0 (0.0) | 1 (100.0) | 0 (0.0) | 0 (0.0) |
χ
2, P
| 5.368, 0.498 | 5.368, 0.498 | 6.642, 0.355 | ||||||
Growth patterns | |||||||||
Expansive | 2 (100.0) | 0 (0.0) | 0 (0.0) | 2 (100.0) | 0 (0.0) | 0 (0.0) | 1 (50.0) | 1 (50.0) | 0 (0.0) |
Invasive | 56 (78.9) | 13 (18.3) | 2 (2.8) | 56 (78.9) | 13 (18.3) | 2 (2.8) | 47 (66.2) | 21 (29.6) | 3 (4.2) |
Expansive + invasive | 23 (92.0) | 2 (8.0) | 0 (0.0) | 23 (92.0) | 2 (8.0) | 0 (0.0) | 14 (56.0) | 10 (40.0) | 1 (4.0) |
χ
2, P
| 3.800, 0.434 | 3.800, 0.434 | 1.295, 0.862 | ||||||
χ
2, P
| 1.239, 0.538 | 1.239, 0.538 | 2.959, 0.228 | ||||||
Depth of invasion | |||||||||
T1 | 11 (78.6) | 1 (7.1) | 2 (14.2) | 11 (78.6) | 1 (7.1) | 2 (14.2) | 11 (78.6) | 3 (21.4) | 0 (0.0) |
T2 | 2 (40.0) | 3 (60.0) | 0 (0.0) | 2 (40.0) | 3 (60.0) | 0 (0.0) | 4 (80.0) | 1 (20.0) | 0 (0.0) |
T3 | 2 (66.7) | 1 (33.3) | 0 (0.0) | 2 (66.7) | 1 (33.3) | 0 (0.0) | 3 (100.0) | 0 (0.0) | 0 (0.0) |
T4 | 66 (86.8) | 10 (13.2) | 0 (0.0) | 66 (86.8) | 10 (13.2) | 0 (0.0) | 44 (57.9) | 28 (36.8) | 4 (5.3) |
χ
2, P
| 14.637, 0.023
| 14.637, 0.023
| 6.870, 0.333 | ||||||
Lymph node metastasis | |||||||||
N0 | 41 (78.8) | 10 (19.2) | 1 (1.9) | 41 (78.8) | 10 (19.2) | 1 (1.9) | 35 (67.3) | 16 (30.8) | 1 (1.9) |
N1 | 26 (81.3) | 5 (15.6) | 1 (3.1) | 26 (81.3) | 5 (15.6) | 1 (3.1) | 21 (65.6) | 9 (28.1) | 2 (6.3) |
N2 | 11 (100) | 0 (0.0) | 0 (0.0) | 11 (100) | 0 (0.0) | 0 (0.0) | 3 (27.3) | 7 (63.6) | 1 (9.1) |
Undetermined | 3 (100.0) | 0 (0.0) | 0 (0.0) | 3 (100.0) | 0 (0.0) | 0 (0.0) | 3 (100.0) | 0 (0.0) | 0 (0.0) |
χ
2, P
| 6.082, 0.414 | 6.082, 0.414 | 10.126, 0.119 | ||||||
Distant metastasis | |||||||||
M0 | 69 (80.2) | 15 (17.4) | 2 (2.3) | 69 (80.2) | 15 (17.4) | 2 (2.3) | 57 (66.3) | 25 (29.1) | 4 (4.7) |
M1 | 12 (100) | 0 (0.0) | 0 (0.0) | 12 (100) | 0 (0.0) | 0 (0.0) | 5 (41.7) | 7 (58.3) | 0 (0.0) |
χ
2, P
| 4.912, 0.086 | 4.912, 0.086 | 4.485, 0.106 | ||||||
TNM staging | |||||||||
I | 13 (72.2) | 3 (16.7) | 2 (11.1) | 13 (72.2) | 3 (16.7) | 2 (11.1) | 14 (77.8) | 4 (22.2) | 0 (0.0) |
IIA-IIC | 23 (79.3) | 6 (20.7) | 0 (0.0) | 23 (79.3) | 6 (20.7) | 0 (0.0) | 22 (75.9) | 6 (20.7) | 1 (3.4) |
IIIA-IIIC | 33 (84.6) | 6 (15.4) | 0 (0.0) | 33 (84.6) | 6 (15.4) | 0 (0.0) | 21 (53.8) | 15 (38.5) | 3 (7.7) |
IVA-IVB | 12 (100) | 0 (0.0) | 0 (0.0) | 12 (100) | 0 (0.0) | 0 (0.0) | 5 (41.7) | 7 (58.3) | 0 (0.0) |
χ
2, P
| 11.682, 0.069 | 11.682, 0.069 | 10.771, 0.096 |
Clinical features | Combination | |||||
---|---|---|---|---|---|---|
TA6/6 −3156GG, 211GG | TA6/6, −3156GG, 211GA | TA6/7, −3156GA, 211GG | TA6/7, −3156GA, 211GA | TA6/6, −3156GG, 211AA | TA7/7, −3156AA, 211GG | |
Gender | ||||||
Male | 39 (51.3) | 21 (27.6) | 8 (10.5) | 2 (2.6) | 4 (5.3) | 2 (2.6) |
Female | 8 (36.4) | 9 (40.9) | 5 (22.7) | 0 (0.0) | 0 (0.0) | 0 (0.0) |
χ
2, P
| 7.516, 0.185 | |||||
Age | ||||||
< 57 years | 19 (46.3) | 13 (31.7) | 7 (17.1) | 1 (2.4) | 1 (2.4) | 0 (0.0) |
> 57 years | 28 (49.1) | 17 (29.8) | 6 (10.5) | 1 (1.8) | 3 (5.3) | 2 (3.5) |
χ
2, P
| 3.541, 0.617 | |||||
Family history | ||||||
Yes | 1 (20.0) | 2 (40.0) | 1 (20.0) | 0 (0.0) | 1 (20.0) | 0 (0.0) |
No | 46 (49.5) | 28 (30.1) | 12 (12.9) | 2 (2.2) | 3 (3.2) | 2 (2.2) |
χ
2, P
| 3.571, 0.613 | |||||
Alcohol history | ||||||
Yes | 18 (56.3) | 9 (28.1) | 3 (9.4) | 0 (0.0) | 2 (6.3) | 0 (0.0) |
No | 29 (43.9) | 21 (31.8) | 10 (15.2) | 2 (3.0) | 2 (3.0) | 2 (3.0) |
χ
2, P
| 7.516, 0.185 | |||||
Histological type | ||||||
Tubular adenocarcinoma | 40 (53.3) | 18 (24.0) | 12 (16.0) | 1 (1.3) | 2 (2.7) | 2 (2.7) |
Non-tubular adenocarcinoma | 7 (30.4) | 12 (52.2) | 1 (4.3) | 1 (4.3) | 2 (8.7) | 0 (0.0) |
χ
2, P
| 11.488, 0.043
| |||||
Differentiation | ||||||
Undifferentiated | 5 (33.3) | 6 (40.0) | 1 (6.7) | 1 (6.7) | 2 (13.3) | 0 (0.0) |
Low | 32 (53.3) | 18 (30.0) | 8 (13.3) | 0 (0.0) | 1 (1.7) | 1 (1.7) |
Moderate | 10 (45.5) | 6 (27.3) | 3 (13.6) | 1 (4.5) | 1 (4.5) | 1 (4.5) |
High | 0 (0.0) | 0 (0.0) | 1 (100.0) | 0 (0.0) | 0 (0.0) | 0 (0.0) |
χ
2, P
| 14.419, 0.494 | |||||
Growth patterns | ||||||
Expansive | 1 (50.0) | 1 (50.0) | 0 (0.0) | 0 (0.0) | 0 (0.0) | 0 (0.0) |
Invasive | 34 (47.9) | 19 (26.8) | 11 (15.5) | 2 (2.8) | 3 (4.2) | 2 (2.8) |
Expansive + invasive | 12 (48.0) | 10 (40.0) | 2 (8.0) | 0 (0.0) | 1 (4.0) | 0 (0.0) |
χ
2, P
| 5.361, 0.866 | |||||
Metastasis | ||||||
Yes | 23 (44.2) | 19 (36.5) | 5 (9.6) | 1 (1.9) | 3 (5.8) | 1 (1.9) |
No | 24 (52.2) | 11 (23.9) | 8 (17.4) | 1 (2.2) | 1 (2.2) | 1 (2.2) |
χ
2, P
| 3.558, 0.615 | |||||
Depth of invasion | ||||||
T1 | 8 (57.1) | 3 (21.4) | 1 (7.1) | 0 (0.0) | 0 (0.0) | 2 (14.3) |
T2 | 1 (20.0) | 1 (20.0) | 3 (60.0) | 0 (0.0) | 0 (0.0) | 0 (0.0) |
T3 | 2 (66.7) | 0 (0.0) | 1 (33.3) | 0 (0.0) | 0 (0.0) | 0 (0.0) |
T4 | 36 (47.4) | 26 (34.2) | 8 (10.5) | 2 (2.6) | 4 (5.3) | 0 (0.0) |
χ
2, P
| 21.061, 0.135 | |||||
Lymph node metastasis | ||||||
N0 | 25 (48.1) | 15 (28.8) | 9 (17.3) | 1 (1.9) | 1 (1.9) | 1 (1.9) |
N1 | 16 (50.0) | 8 (25.0) | 4 (12.5) | 1 (3.1) | 2 (6.3) | 1 (3.1) |
N2 | 3 (27.3) | 7 (63.6) | 0 (0.0) | 0 (0.0) | 1 (9.1) | 0 (0.0) |
Undetermined | 3 (100.0) | 0 (0.0) | 0 (0.0) | 0 (0.0) | 0 (0.0) | 0 (0.0) |
χ
2, P
| 15.280, 0.431 | |||||
Distant metastasis | ||||||
M0 | 42 (48.8) | 23 (26.7) | 13 (15.1) | 2 (2.3) | 4 (4.7) | 2 (2.3) |
M1 | 5 (41.7) | 7 (58.3) | 0 (0.0) | 0 (0.0) | 0 (0.0) | 0 (0.0) |
χ
2, P
| 8.417, 0.135 | |||||
TNM staging | ||||||
I | 9 (50.0) | 4 (22.2) | 3 (16.7) | 0 (0.0) | 0 (0.0) | 2 (11.1) |
IIA-IIC | 16 (55.2) | 6 (20.7) | 6 (20.7) | 0 (0.0) | 1 (3.4) | 0 (0.0) |
IIIA-IIIC | 17 (43.6) | 13 (33.3) | 4 (10.3) | 2 (5.1) | 3 (7.7) | 0 (0.0) |
IVA-IVB | 5 (41.7) | 7 (58.3) | 0 (0.0) | 0 (2.6) | 0 (5.3) | 0 (0.0) |
χ
2, P
| 23.131, 0.081 |